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The map label for this gene is brnQ [H]

Identifier: 52143505

GI number: 52143505

Start: 1852324

End: 1853682

Strand: Direct

Name: brnQ [H]

Synonym: BCZK1729

Alternate gene names: 52143505

Gene position: 1852324-1853682 (Clockwise)

Preceding gene: 52143506

Following gene: 52143503

Centisome position: 34.94

GC content: 36.28

Gene sequence:

>1359_bases
ATGACGAATAAAGTACCGTTTTCGTTCATAGTTGTTATTGGTTTAATGTTATTTGCACTATTTTTTGGGGCAGGAAATTT
AATATTCCCAGCAATGCTTGGTCAATCGGCAGGAGAGAATGTGTGGATTGCTAATGCTGGATTTTTAGTAACAGGTGTAG
GTTTACCGTTGCTAGGGGTATTAGCATTTGGAATTTCAGGTAAAGAAGATTTACAATCGTTAGCAAGTCGTGTACATCCA
GTGTTCGGCATTGTATTTACGACAGTTTTATATTTAGCGATTGGTCCACTATTCGCTATTCCGAGAACAGGCAGCGTTTC
TTATGAAATTGGTATGAAGCCTTTTGTACCAGAAGCTATGGGTTCTTCAGCACTCATTCTTTTTACAATTATATTCTTTA
GCATTACTTGTTTTTTTTCGCTAAATCCCGCGAAAATTGTCGATATTGTAGGAAAAATCTTAACGCCAATTAAATTAACG
TTTATTGGGATTTTAGTAGTATTTGCTTTTATTCATCCAATGGGAGAAATGCAAGCACCTGCAGAGGCATATACATCACA
TGCGTTCTTTAAAGGGTTCCAAGAAGGTTACTTAACAATGGATACGTTAGCATCATTCGTATTCGGTATTATCATCATCA
ATGCGATTAAAGAAAAGGGCGCAAAAACAAAAAAAGACATTATGATTGTTTGTGCAAAAGCAACATTGATTGCTGCATCT
ATTTTAGCAATGATCTATTCAGCACTTTCATTTATGGGAGCTTCAAGTGTTGCAAAACTTGGTCATTTAGATAATGGTGG
TGCAGTATTAGCAAAAGTTTCAAACTATTATTTTGGGTCATACGGCGGAATTATATTAGGGATTATGGTTACAGCAGCAT
GTTTAACGACTAGTGTAGGACTTGTTTCAGCGTGTTCTTCATTCTTCCATAAACTATTCCCGAATGTTTCGTATAAAAAG
ATAGCAATCATCTTATCAGTGTTCAGTGCAGTTGTTGCGAATGTAGGTTTAACGCAATTAATCGCAATTTCTGTTCCTGT
ATTAATCGCGATTTATCCATTAGCAATCGTCTTAATTTTCCTAACGCTCCTTCATCCATTGTTTAAAGGACGAGCAGAAG
TGTATCAAGGTAGCTTGTTAATTACATTTATTGTAAGTGTGTTCGATGGATTGAATGCAGCTGGTATGAAGTTAGCAGTA
GTAAATGATTTTCTTACTAAAATACTTCCAATGCATGAAGTTGGGTTAGGTTGGATTTTACCCGCACTTATTGGCGGATT
GATCGGGTACGTCATTAGCATTGTGAAAGCAAAAAATCAAGTTCAACCAGCAGCTAGTGCGGATAAGAAAATAGGTTAA

Upstream 100 bases:

>100_bases
TAATGAGTTCAAGAATAGTTGATACTCAAGTTAAATATTCAGAAAATTCAATTAGTTAAAAATGGAAATGACATAGGTTA
TTTTGAATGGAGGTTATATT

Downstream 100 bases:

>100_bases
ATAAAAAAGTTATAGAACTTGTTTCTATAACTTTTTTTGTTACTATTAATAATGAGAGAAAATAAAGTAGTAGGGGAAAT
AAAATGAAAAAAACAATTGA

Product: branched-chain amino acid transport system carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MTNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVGLPLLGVLAFGISGKEDLQSLASRVHP
VFGIVFTTVLYLAIGPLFAIPRTGSVSYEIGMKPFVPEAMGSSALILFTIIFFSITCFFSLNPAKIVDIVGKILTPIKLT
FIGILVVFAFIHPMGEMQAPAEAYTSHAFFKGFQEGYLTMDTLASFVFGIIIINAIKEKGAKTKKDIMIVCAKATLIAAS
ILAMIYSALSFMGASSVAKLGHLDNGGAVLAKVSNYYFGSYGGIILGIMVTAACLTTSVGLVSACSSFFHKLFPNVSYKK
IAIILSVFSAVVANVGLTQLIAISVPVLIAIYPLAIVLIFLTLLHPLFKGRAEVYQGSLLITFIVSVFDGLNAAGMKLAV
VNDFLTKILPMHEVGLGWILPALIGGLIGYVISIVKAKNQVQPAASADKKIG

Sequences:

>Translated_452_residues
MTNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVGLPLLGVLAFGISGKEDLQSLASRVHP
VFGIVFTTVLYLAIGPLFAIPRTGSVSYEIGMKPFVPEAMGSSALILFTIIFFSITCFFSLNPAKIVDIVGKILTPIKLT
FIGILVVFAFIHPMGEMQAPAEAYTSHAFFKGFQEGYLTMDTLASFVFGIIIINAIKEKGAKTKKDIMIVCAKATLIAAS
ILAMIYSALSFMGASSVAKLGHLDNGGAVLAKVSNYYFGSYGGIILGIMVTAACLTTSVGLVSACSSFFHKLFPNVSYKK
IAIILSVFSAVVANVGLTQLIAISVPVLIAIYPLAIVLIFLTLLHPLFKGRAEVYQGSLLITFIVSVFDGLNAAGMKLAV
VNDFLTKILPMHEVGLGWILPALIGGLIGYVISIVKAKNQVQPAASADKKIG
>Mature_451_residues
TNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVGLPLLGVLAFGISGKEDLQSLASRVHPV
FGIVFTTVLYLAIGPLFAIPRTGSVSYEIGMKPFVPEAMGSSALILFTIIFFSITCFFSLNPAKIVDIVGKILTPIKLTF
IGILVVFAFIHPMGEMQAPAEAYTSHAFFKGFQEGYLTMDTLASFVFGIIIINAIKEKGAKTKKDIMIVCAKATLIAASI
LAMIYSALSFMGASSVAKLGHLDNGGAVLAKVSNYYFGSYGGIILGIMVTAACLTTSVGLVSACSSFFHKLFPNVSYKKI
AIILSVFSAVVANVGLTQLIAISVPVLIAIYPLAIVLIFLTLLHPLFKGRAEVYQGSLLITFIVSVFDGLNAAGMKLAVV
NDFLTKILPMHEVGLGWILPALIGGLIGYVISIVKAKNQVQPAASADKKIG

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=429, Percent_Identity=40.7925407925408, Blast_Score=302, Evalue=4e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 47873; Mature: 47742

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVGLPLLGV
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHH
LAFGISGKEDLQSLASRVHPVFGIVFTTVLYLAIGPLFAIPRTGSVSYEIGMKPFVPEAM
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHC
GSSALILFTIIFFSITCFFSLNPAKIVDIVGKILTPIKLTFIGILVVFAFIHPMGEMQAP
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AEAYTSHAFFKGFQEGYLTMDTLASFVFGIIIINAIKEKGAKTKKDIMIVCAKATLIAAS
HHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHH
ILAMIYSALSFMGASSVAKLGHLDNGGAVLAKVSNYYFGSYGGIILGIMVTAACLTTSVG
HHHHHHHHHHHHCHHHHHHHCCCCCCCCEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LVSACSSFFHKLFPNVSYKKIAIILSVFSAVVANVGLTQLIAISVPVLIAIYPLAIVLIF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LTLLHPLFKGRAEVYQGSLLITFIVSVFDGLNAAGMKLAVVNDFLTKILPMHEVGLGWIL
HHHHHHHHCCHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
PALIGGLIGYVISIVKAKNQVQPAASADKKIG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVGLPLLGV
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHH
LAFGISGKEDLQSLASRVHPVFGIVFTTVLYLAIGPLFAIPRTGSVSYEIGMKPFVPEAM
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHC
GSSALILFTIIFFSITCFFSLNPAKIVDIVGKILTPIKLTFIGILVVFAFIHPMGEMQAP
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AEAYTSHAFFKGFQEGYLTMDTLASFVFGIIIINAIKEKGAKTKKDIMIVCAKATLIAAS
HHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHH
ILAMIYSALSFMGASSVAKLGHLDNGGAVLAKVSNYYFGSYGGIILGIMVTAACLTTSVG
HHHHHHHHHHHHCHHHHHHHCCCCCCCCEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LVSACSSFFHKLFPNVSYKKIAIILSVFSAVVANVGLTQLIAISVPVLIAIYPLAIVLIF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LTLLHPLFKGRAEVYQGSLLITFIVSVFDGLNAAGMKLAVVNDFLTKILPMHEVGLGWIL
HHHHHHHHCCHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
PALIGGLIGYVISIVKAKNQVQPAASADKKIG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]