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The map label for this gene is gatA [H]

Identifier: 52143496

GI number: 52143496

Start: 1862130

End: 1863740

Strand: Direct

Name: gatA [H]

Synonym: BCZK1739

Alternate gene names: 52143496

Gene position: 1862130-1863740 (Clockwise)

Preceding gene: 52143495

Following gene: 52143494

Centisome position: 35.13

GC content: 35.69

Gene sequence:

>1611_bases
ATGAAGAAATGGGTAAAGGTAACGCTGTCTATCACTGGAGGTATTGTCTTATTAGCTTGTGCCGGCGGATATTACGTATA
TAAAAATTATTTTCCAAAGGAACCTGAGCGTATTGTATATGATAAAGAAAGAGTGCTACAACCGATACATAATCAGCTTA
AAGGGGTCAATATAGAAAATGTAAAGATAAAAGAAAAAGAAGTTGTAAATGCGACTGTAGATGAGCTTCAAAAAATGATT
GATGATGGAAAATTATCATATGAAGAATTAACGAGTATTTATCTCTTTAGAATACAAGAACATGATCAAAATGGAATAAC
ATTAAATTCTGTTACAGAGATAAATCCTAATGCGATGGAAGAAGCAAGAAAGTTAGATCAAGAGCGAGGTAGAAACAAAA
AGTCGAATTTATATGGTATTCCTGTCGTTGTAAAAGATAATGTACAAACGGCAAAAGTGATGCCGACTAGTGCAGGAACT
TATGTATTAAAAGATTGGATTGCAGATCAAGACGCAACAATTGTGAAGCAGTTGAAAGAAGAAGGGGCATTTGTTTTAGG
AAAAGCGAATATGTCTGAGTGGGCGAATTATTTATCTTTTACAATGCCAAGCGGGTATAGCGGGAAGAAAGGACAAAATT
TAAATCCGTACGATCCGATTACATTCGATACTTCAGGATCGAGTTCAGGATCTGCTACAGTAGTTGCAGCAGATTTTGCA
CCGCTTGCAGTCGGAACAGAAACGACAGGATCGATTGTAGCACCCGCAGCGCAGCAATCAGTAGTTGGGTTACGTCCATC
TTTAGGTATGGTAAGTAGAACAGGAATTATTCCGTTAGCTGAAACTCTTGATACAGCAGGACCTATGGCAAGAACGGTAA
AAGATGCAGCAACGTTATTTAATGCAATGATAGGTTATGACGAAAAAGATGTTATGACAGAAAAAGTGAAAGATAAAGAA
AGAATTGATTATACAAAGGATTTATCAATAGATGGATTAAAAGGGAAAAAGATAGGGCTTCTTTTTTCTGTAGACCAACA
AGATGAAAATAGAAAAGCAGTAGCAGAAAAGATTAGAAAAGACCTTCAAGATGCAGGTGCGATATTAACTGATTATATTC
AGTTAAACAATGGGGGTGTAGATAATTTACAAACATTAGAATATGAATTCAAGCATAATGTAAACGACTATTTTTCACAG
CAAAAAAATGTGCCGGTAAAATCGTTAGAAGAGATTATAGCGTTTAATAAAAAGGATAGTAAAAGACGAATAAAATACGG
ACAAACATTAATTGAGGAGTCTGAAAAATCTGCAATAACGAAAGATGAGTTTGAAAAAGTAGTGCAAACGAGTCAAGAAA
ATGCAAAAAAAGAGCTAGATAGATATTTAGTAGAAAAGGGTTTAGACGCTTTAGTTATGATTAACAACGAGGAAGTTCTT
CTATCCGCTGTAGCTGGCTATCCAGAATTAGCGGTGCCAGCAGGATATGATAACAATGGAGAGCCAGTAGGTGCAGTATT
TGTCGGGAAGCAATTCAGTGAGAAGGAACTGTTCAATATTGGATATGCGTATGAACAGCAGTCTAAAAATAGAAAACCAC
CTAAACTGTAA

Upstream 100 bases:

>100_bases
AATTCTTCATTTTTTCATTTAGCATTTTTATTAAAGAGAGTTATAAAGATAACTCTCTTTTTCAATACAATTGAGCAACT
GTTAGAGTGGAGGAAGCAAC

Downstream 100 bases:

>100_bases
AAGGTTAAACGAAAAAAAGAGCTCAACTGAAGTAAGTAGGTGGGCTAATATAAATAGAATCTAACAATATAAAAAGAAAA
AAAGGAATCTTTCTAATTAG

Product: amidase

Products: ADP; phosphate; Gln-tRNA(Gln); L-glutamate

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MKKWVKVTLSITGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGVNIENVKIKEKEVVNATVDELQKMI
DDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAMEEARKLDQERGRNKKSNLYGIPVVVKDNVQTAKVMPTSAGT
YVLKDWIADQDATIVKQLKEEGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYDPITFDTSGSSSGSATVVAADFA
PLAVGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLFNAMIGYDEKDVMTEKVKDKE
RIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRKDLQDAGAILTDYIQLNNGGVDNLQTLEYEFKHNVNDYFSQ
QKNVPVKSLEEIIAFNKKDSKRRIKYGQTLIEESEKSAITKDEFEKVVQTSQENAKKELDRYLVEKGLDALVMINNEEVL
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFSEKELFNIGYAYEQQSKNRKPPKL

Sequences:

>Translated_536_residues
MKKWVKVTLSITGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGVNIENVKIKEKEVVNATVDELQKMI
DDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAMEEARKLDQERGRNKKSNLYGIPVVVKDNVQTAKVMPTSAGT
YVLKDWIADQDATIVKQLKEEGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYDPITFDTSGSSSGSATVVAADFA
PLAVGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLFNAMIGYDEKDVMTEKVKDKE
RIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRKDLQDAGAILTDYIQLNNGGVDNLQTLEYEFKHNVNDYFSQ
QKNVPVKSLEEIIAFNKKDSKRRIKYGQTLIEESEKSAITKDEFEKVVQTSQENAKKELDRYLVEKGLDALVMINNEEVL
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFSEKELFNIGYAYEQQSKNRKPPKL
>Mature_536_residues
MKKWVKVTLSITGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGVNIENVKIKEKEVVNATVDELQKMI
DDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAMEEARKLDQERGRNKKSNLYGIPVVVKDNVQTAKVMPTSAGT
YVLKDWIADQDATIVKQLKEEGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYDPITFDTSGSSSGSATVVAADFA
PLAVGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLFNAMIGYDEKDVMTEKVKDKE
RIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRKDLQDAGAILTDYIQLNNGGVDNLQTLEYEFKHNVNDYFSQ
QKNVPVKSLEEIIAFNKKDSKRRIKYGQTLIEESEKSAITKDEFEKVVQTSQENAKKELDRYLVEKGLDALVMINNEEVL
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFSEKELFNIGYAYEQQSKNRKPPKL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=258, Percent_Identity=32.9457364341085, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI195972892, Length=247, Percent_Identity=32.7935222672065, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17543272, Length=462, Percent_Identity=24.4588744588745, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17537465, Length=519, Percent_Identity=22.3506743737958, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=256, Percent_Identity=25.390625, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=221, Percent_Identity=35.2941176470588, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319685, Length=177, Percent_Identity=33.8983050847458, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648113, Length=507, Percent_Identity=25.6410256410256, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24644968, Length=516, Percent_Identity=22.6744186046512, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356731, Length=277, Percent_Identity=24.5487364620939, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI161078093, Length=513, Percent_Identity=23.5867446393762, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.7

Molecular weight: Translated: 59537; Mature: 59537

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWVKVTLSITGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGVNIEN
CCCEEEEEEEECCCEEEEEECCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCC
VKIKEKEVVNATVDELQKMIDDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAME
EEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEEEEECCCCCEEEECEECCCCHHHH
EARKLDQERGRNKKSNLYGIPVVVKDNVQTAKVMPTSAGTYVLKDWIADQDATIVKQLKE
HHHHHHHHHCCCCCCCEEEEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHH
EGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYDPITFDTSGSSSGSATVVAADFA
CCCEEEECCCHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCC
PLAVGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLF
CEEECCCCCCCEECCCHHHHHHCCCCCCCCHHCCCCCCHHHHHHCCCHHHHHHHHHHHHH
NAMIGYDEKDVMTEKVKDKERIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRK
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
DLQDAGAILTDYIQLNNGGVDNLQTLEYEFKHNVNDYFSQQKNVPVKSLEEIIAFNKKDS
HHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCCCCC
KRRIKYGQTLIEESEKSAITKDEFEKVVQTSQENAKKELDRYLVEKGLDALVMINNEEVL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEE
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFSEKELFNIGYAYEQQSKNRKPPKL
EHHHCCCCCEECCCCCCCCCCEEEEEEECCCCCCHHHHHCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKWVKVTLSITGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGVNIEN
CCCEEEEEEEECCCEEEEEECCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCC
VKIKEKEVVNATVDELQKMIDDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAME
EEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEEEEECCCCCEEEECEECCCCHHHH
EARKLDQERGRNKKSNLYGIPVVVKDNVQTAKVMPTSAGTYVLKDWIADQDATIVKQLKE
HHHHHHHHHCCCCCCCEEEEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHH
EGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYDPITFDTSGSSSGSATVVAADFA
CCCEEEECCCHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCC
PLAVGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLF
CEEECCCCCCCEECCCHHHHHHCCCCCCCCHHCCCCCCHHHHHHCCCHHHHHHHHHHHHH
NAMIGYDEKDVMTEKVKDKERIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRK
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
DLQDAGAILTDYIQLNNGGVDNLQTLEYEFKHNVNDYFSQQKNVPVKSLEEIIAFNKKDS
HHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCCCCC
KRRIKYGQTLIEESEKSAITKDEFEKVVQTSQENAKKELDRYLVEKGLDALVMINNEEVL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEE
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFSEKELFNIGYAYEQQSKNRKPPKL
EHHHCCCCCEECCCCCCCCCCEEEEEEECCCCCCHHHHHCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Glu-tRNA(Gln); L-glutamine

Specific reaction: ATP + Glu-tRNA(Gln) + L-glutamine = ADP + phosphate + Gln-tRNA(Gln) + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]