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The map label for this gene is cls [H]

Identifier: 52143372

GI number: 52143372

Start: 1984921

End: 1986024

Strand: Direct

Name: cls [H]

Synonym: BCZK1860

Alternate gene names: 52143372

Gene position: 1984921-1986024 (Clockwise)

Preceding gene: 52143376

Following gene: 52143373

Centisome position: 37.44

GC content: 34.69

Gene sequence:

>1104_bases
ATGGCTAGTGAATATGAAGTAGGACAAGTTAGATTAGGTGAATTTCAACTGTATACGAACGGTGAAGAGTTATATACGAA
TTTATTTGAGGATATACGTAATGCGGAGAAGTACATATATATCCATTTTTATATTGTAGGTAAAGATGAAATAAGCAAAG
AGTTTTTGCAGTTGCTCGAGAAAAAGGCATCGGGTGGAGTAGAAGTGAAATTGTCAGTCGATCGAATAGGTGGATATAAG
CTGAAGAAAAAGGTAATTCGTCAACTACAAAGCAATGGTGTCCAGTTTACGTTTAGTAAAAAACCTAAATTGAAACATTT
ATTCTATTCGTTGCATCAGCGAAATCATAGACGAATTGTTACGATAGACGGAAAAGTATCATATATCGGCGGCTTTAACA
TCGGAAAGGAATACCTTGGACAAGATCCGAAATTTGGCCCGTGGCGTGATTATCATGTGCGAGTTAAGGGGAATGGTGCC
ACGGATATGGAAAGAAAATTTGCTGCTGACTGGAAAGAAGATACGGGAGAAAAAATGCCTGTACATGAAAGTCTACCTGC
AATTGGGAATGTAAAATATCAATATTTATTTTCAGATGGAAAAGGGTTATGGGAAAAATATGGAGCGTTTTTAAAGCAGG
CTAAAAAGTCTTTAATTATTGCTACACCATATTTTGTACCAAGTAAAGAAATGATGAAAGAGTTAAAAGGTGCATTAAAC
CGCGGTGTCAATGTGAAAATTCTCGTACCATTTAAAAGTGATGCAGTATTGTTAAAACAAGCTGCCTATCCGTATTTGAG
AGAGATGAACCATGCTGGAGCGGAGATTTATCAATATCGGAATGGTTTTTTTCATGGGAAAGTAACAATCATTGATGGAG
AAATTGTTGATATAGGTACAGCGAATTTTGATAATAGAAGTTTTTATTTAAATTGTGAATCTAACTGTGTCATATATGAT
AGAACAGTTGTTGCTGACGTATGGGATCGATTAAAAGAAGACTTCCATAAATCAAAAAGATTTTCGGAAGAAGATTTTGA
GAAAATTAGTAAATGGGATTGGTTTTTAGCAAGAATAGCAAATGTTATAGCATCATATTTATAG

Upstream 100 bases:

>100_bases
TGGAGAAAGGAAAATATCCATGAAACACTTTTTCGTTGTAATCCTTTGTGTAATAGGTGTATTCATTTGGATGAACATCG
ATGTGGAAATGGGGAAAAAA

Downstream 100 bases:

>100_bases
CATATAAACAAAAAGTAGGGAGGCACTTTCATGGATTGTATGAAAGAATTGATGAGGTATAGTTATATGCTTATATATAA
GGTTGGGGAGTATACAGGAC

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MASEYEVGQVRLGEFQLYTNGEELYTNLFEDIRNAEKYIYIHFYIVGKDEISKEFLQLLEKKASGGVEVKLSVDRIGGYK
LKKKVIRQLQSNGVQFTFSKKPKLKHLFYSLHQRNHRRIVTIDGKVSYIGGFNIGKEYLGQDPKFGPWRDYHVRVKGNGA
TDMERKFAADWKEDTGEKMPVHESLPAIGNVKYQYLFSDGKGLWEKYGAFLKQAKKSLIIATPYFVPSKEMMKELKGALN
RGVNVKILVPFKSDAVLLKQAAYPYLREMNHAGAEIYQYRNGFFHGKVTIIDGEIVDIGTANFDNRSFYLNCESNCVIYD
RTVVADVWDRLKEDFHKSKRFSEEDFEKISKWDWFLARIANVIASYL

Sequences:

>Translated_367_residues
MASEYEVGQVRLGEFQLYTNGEELYTNLFEDIRNAEKYIYIHFYIVGKDEISKEFLQLLEKKASGGVEVKLSVDRIGGYK
LKKKVIRQLQSNGVQFTFSKKPKLKHLFYSLHQRNHRRIVTIDGKVSYIGGFNIGKEYLGQDPKFGPWRDYHVRVKGNGA
TDMERKFAADWKEDTGEKMPVHESLPAIGNVKYQYLFSDGKGLWEKYGAFLKQAKKSLIIATPYFVPSKEMMKELKGALN
RGVNVKILVPFKSDAVLLKQAAYPYLREMNHAGAEIYQYRNGFFHGKVTIIDGEIVDIGTANFDNRSFYLNCESNCVIYD
RTVVADVWDRLKEDFHKSKRFSEEDFEKISKWDWFLARIANVIASYL
>Mature_366_residues
ASEYEVGQVRLGEFQLYTNGEELYTNLFEDIRNAEKYIYIHFYIVGKDEISKEFLQLLEKKASGGVEVKLSVDRIGGYKL
KKKVIRQLQSNGVQFTFSKKPKLKHLFYSLHQRNHRRIVTIDGKVSYIGGFNIGKEYLGQDPKFGPWRDYHVRVKGNGAT
DMERKFAADWKEDTGEKMPVHESLPAIGNVKYQYLFSDGKGLWEKYGAFLKQAKKSLIIATPYFVPSKEMMKELKGALNR
GVNVKILVPFKSDAVLLKQAAYPYLREMNHAGAEIYQYRNGFFHGKVTIIDGEIVDIGTANFDNRSFYLNCESNCVIYDR
TVVADVWDRLKEDFHKSKRFSEEDFEKISKWDWFLARIANVIASYL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=350, Percent_Identity=28.8571428571429, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1787007, Length=320, Percent_Identity=27.1875, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 42528; Mature: 42397

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASEYEVGQVRLGEFQLYTNGEELYTNLFEDIRNAEKYIYIHFYIVGKDEISKEFLQLLE
CCCCCCCCEEEECEEEEEECHHHHHHHHHHHHCCCCEEEEEEEEEECCHHHHHHHHHHHH
KKASGGVEVKLSVDRIGGYKLKKKVIRQLQSNGVQFTFSKKPKLKHLFYSLHQRNHRRIV
HHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCCCCEEE
TIDGKVSYIGGFNIGKEYLGQDPKFGPWRDYHVRVKGNGATDMERKFAADWKEDTGEKMP
EECCEEEEEECCCCCHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHCCCHHCCCCCCC
VHESLPAIGNVKYQYLFSDGKGLWEKYGAFLKQAKKSLIIATPYFVPSKEMMKELKGALN
CHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHC
RGVNVKILVPFKSDAVLLKQAAYPYLREMNHAGAEIYQYRNGFFHGKVTIIDGEIVDIGT
CCCEEEEEEEECCCHHHHHHHCCHHHHHHCCCHHHHHHHHCCEEEEEEEEEECEEEEECC
ANFDNRSFYLNCESNCVIYDRTVVADVWDRLKEDFHKSKRFSEEDFEKISKWDWFLARIA
CCCCCCEEEEEECCCEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
NVIASYL
HHHHHCC
>Mature Secondary Structure 
ASEYEVGQVRLGEFQLYTNGEELYTNLFEDIRNAEKYIYIHFYIVGKDEISKEFLQLLE
CCCCCCCEEEECEEEEEECHHHHHHHHHHHHCCCCEEEEEEEEEECCHHHHHHHHHHHH
KKASGGVEVKLSVDRIGGYKLKKKVIRQLQSNGVQFTFSKKPKLKHLFYSLHQRNHRRIV
HHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCCCCEEE
TIDGKVSYIGGFNIGKEYLGQDPKFGPWRDYHVRVKGNGATDMERKFAADWKEDTGEKMP
EECCEEEEEECCCCCHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHCCCHHCCCCCCC
VHESLPAIGNVKYQYLFSDGKGLWEKYGAFLKQAKKSLIIATPYFVPSKEMMKELKGALN
CHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHC
RGVNVKILVPFKSDAVLLKQAAYPYLREMNHAGAEIYQYRNGFFHGKVTIIDGEIVDIGT
CCCEEEEEEEECCCHHHHHHHCCHHHHHHCCCHHHHHHHHCCEEEEEEEEEECEEEEECC
ANFDNRSFYLNCESNCVIYDRTVVADVWDRLKEDFHKSKRFSEEDFEKISKWDWFLARIA
CCCCCCEEEEEECCCEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
NVIASYL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]