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The map label for this gene is gatA [H]

Identifier: 52143359

GI number: 52143359

Start: 1998065

End: 1999540

Strand: Direct

Name: gatA [H]

Synonym: BCZK1877

Alternate gene names: 52143359

Gene position: 1998065-1999540 (Clockwise)

Preceding gene: 52143361

Following gene: 52143357

Centisome position: 37.69

GC content: 35.37

Gene sequence:

>1476_bases
ATGAAGATGCAGTTCAATACACTATTACAAAAAGAATTAACAATTCATGATATACAAAAAGCGATGGAAGATGAAAAGTT
AACTTCAAAAGAATTAGTTATGTATTATCTTCATAGAGTTGCAAAGTATGATCAAGACGGACCGAAAATTAATTCTATTT
TAGAAATAAATCCAGACGCTATTTTTATTGCAGAAGCGTTAGATCATGAAAGAAAGATTAAAGGTGTAAGAGGTCCATTA
CATGGCATACCGGTTTTACTTAAAGACAATATTGAAACGAACGACTCTATGCATACAAGCGCAGGTACGATTGCTCTAGA
ACAAAATATAAGTAGTGAAGATGCATTTCTCGTTACGAAGCTGAGAGAAGCAGGAGCAGTGATACTCGGGAAAACAAATA
TGACAGAATTAGCAAATGCAATGTCTTTTGAAATGTGGGCTGGATATAGTGCAAGAGGTGGTCAAACAATCAATCCTTAC
GGTACAGGCGACGATGATATGATTGTTGGTGGCTCAAGTACAGGATCTGCGATAGCAGTTGCTTCTAACTTTACAGTAGT
ATCAGTTGGAACAGAAACGGATGGTTCTATTTTGAGTCCAGCTGTTCAAAATTCTGTAGTAGGTATTAAACCGACTGTTG
GTTTAATTAGTCGTAGAGGTATTATTCCGTTCACATATTCGCAAGATACAGCCGGTCCATTTGCTAGAACCGTAACAGAT
GCAGCAATTTTACTAGGGAGTTTAACTGGTATAGATGAGAAAGATGTAGCTACTCATAAAAGTGAAGGTATAGCAGAACA
TGATTATACAAAATATCTTGATGTTAATGGATTACATGGAGCAAAGATTGGTGTATATAGTAATGCACCAAAAGATTATT
ATGAAACTGGTGAGTATGATGAAAAATTGTTTAAGGAAACGATTGAAGTATTGCGTAGTGAGGGAGCGACAGTAGTAGAA
GGTATTGATATTCCTTCTTTTCATAGAGAATGGAGCTGGGGAGTATCGCTCTATGAATTAAAGCATAGTTTAGATAACTA
TCTTTCGAAATTACCTTCTACTATGCCAGTGCATTCTATTTCAGAGTTAATGGAGTTTAATGAAAATATAGCAGAAAGAG
CTCTGAAATATGGACAAACTAAGTTAGAGAGAAGAAAAGATTTTCCTAATACGTTAAGAAATCCAGAATATTTACATGCA
AGATTAGAAGATATATATTTTTCGCAAGAACAAGGTATTGATTTTGCACTGAAAAAATATAATCTTGATGCGATATTGTT
CCCTTCTTATATAGGTTCTACTATATGTGCGAAAGCAGGTTATCCGTCTATAGCAATACCTGCAGGATATATGGAAGACG
GGAGACCCTTTGGAATTACTATTGCAAGTACTGCATTTAGTGAAGGGACATTAATTAAACTAGCATACGCTTTTGAACAA
GCGACAAAGCATAGGGAAATCCCAGGATTGTCATAA

Upstream 100 bases:

>100_bases
ATTCTTATAGAGGGATTGTAGTTGTATATGTGAGGAGCGGAGAAAATGAAAAAGTAATAGAGATATGCTATCAATAAAGG
GTTATAGAAGGAGAATGAAA

Downstream 100 bases:

>100_bases
ATAGAATATACAAAAAAGGACAGGAATCCCTGTCCTTTTTGCTTTATTGATTACATTGAAATATCTTTCTGATTTGTTTT
ATGACATTTTAATAGGCTAA

Product: amidase

Products: ADP; phosphate; Gln-tRNA(Gln); L-glutamate

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKMQFNTLLQKELTIHDIQKAMEDEKLTSKELVMYYLHRVAKYDQDGPKINSILEINPDAIFIAEALDHERKIKGVRGPL
HGIPVLLKDNIETNDSMHTSAGTIALEQNISSEDAFLVTKLREAGAVILGKTNMTELANAMSFEMWAGYSARGGQTINPY
GTGDDDMIVGGSSTGSAIAVASNFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
AAILLGSLTGIDEKDVATHKSEGIAEHDYTKYLDVNGLHGAKIGVYSNAPKDYYETGEYDEKLFKETIEVLRSEGATVVE
GIDIPSFHREWSWGVSLYELKHSLDNYLSKLPSTMPVHSISELMEFNENIAERALKYGQTKLERRKDFPNTLRNPEYLHA
RLEDIYFSQEQGIDFALKKYNLDAILFPSYIGSTICAKAGYPSIAIPAGYMEDGRPFGITIASTAFSEGTLIKLAYAFEQ
ATKHREIPGLS

Sequences:

>Translated_491_residues
MKMQFNTLLQKELTIHDIQKAMEDEKLTSKELVMYYLHRVAKYDQDGPKINSILEINPDAIFIAEALDHERKIKGVRGPL
HGIPVLLKDNIETNDSMHTSAGTIALEQNISSEDAFLVTKLREAGAVILGKTNMTELANAMSFEMWAGYSARGGQTINPY
GTGDDDMIVGGSSTGSAIAVASNFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
AAILLGSLTGIDEKDVATHKSEGIAEHDYTKYLDVNGLHGAKIGVYSNAPKDYYETGEYDEKLFKETIEVLRSEGATVVE
GIDIPSFHREWSWGVSLYELKHSLDNYLSKLPSTMPVHSISELMEFNENIAERALKYGQTKLERRKDFPNTLRNPEYLHA
RLEDIYFSQEQGIDFALKKYNLDAILFPSYIGSTICAKAGYPSIAIPAGYMEDGRPFGITIASTAFSEGTLIKLAYAFEQ
ATKHREIPGLS
>Mature_491_residues
MKMQFNTLLQKELTIHDIQKAMEDEKLTSKELVMYYLHRVAKYDQDGPKINSILEINPDAIFIAEALDHERKIKGVRGPL
HGIPVLLKDNIETNDSMHTSAGTIALEQNISSEDAFLVTKLREAGAVILGKTNMTELANAMSFEMWAGYSARGGQTINPY
GTGDDDMIVGGSSTGSAIAVASNFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
AAILLGSLTGIDEKDVATHKSEGIAEHDYTKYLDVNGLHGAKIGVYSNAPKDYYETGEYDEKLFKETIEVLRSEGATVVE
GIDIPSFHREWSWGVSLYELKHSLDNYLSKLPSTMPVHSISELMEFNENIAERALKYGQTKLERRKDFPNTLRNPEYLHA
RLEDIYFSQEQGIDFALKKYNLDAILFPSYIGSTICAKAGYPSIAIPAGYMEDGRPFGITIASTAFSEGTLIKLAYAFEQ
ATKHREIPGLS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=525, Percent_Identity=26.0952380952381, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI195972892, Length=251, Percent_Identity=29.4820717131474, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=460, Percent_Identity=25.2173913043478, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17537465, Length=504, Percent_Identity=23.2142857142857, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=258, Percent_Identity=27.906976744186, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=415, Percent_Identity=27.710843373494, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323950, Length=209, Percent_Identity=37.799043062201, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320448, Length=260, Percent_Identity=25.7692307692308, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24648113, Length=509, Percent_Identity=27.1119842829077, Blast_Score=122, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24648435, Length=256, Percent_Identity=30.078125, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24648437, Length=256, Percent_Identity=30.078125, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI45550774, Length=256, Percent_Identity=30.078125, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24644968, Length=519, Percent_Identity=23.121387283237, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21356731, Length=253, Percent_Identity=29.6442687747036, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24652985, Length=502, Percent_Identity=23.7051792828685, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19922090, Length=502, Percent_Identity=23.7051792828685, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24652981, Length=502, Percent_Identity=23.7051792828685, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24652983, Length=502, Percent_Identity=23.7051792828685, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648441, Length=176, Percent_Identity=32.3863636363636, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648439, Length=176, Percent_Identity=32.3863636363636, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.7

Molecular weight: Translated: 54034; Mature: 54034

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMQFNTLLQKELTIHDIQKAMEDEKLTSKELVMYYLHRVAKYDQDGPKINSILEINPDA
CCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCE
IFIAEALDHERKIKGVRGPLHGIPVLLKDNIETNDSMHTSAGTIALEQNISSEDAFLVTK
EEEEHHHCHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCCCHHHHEE
LREAGAVILGKTNMTELANAMSFEMWAGYSARGGQTINPYGTGDDDMIVGGSSTGSAIAV
HHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCEEEECCCCCCEEEE
ASNFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
ECCEEEEEECCCCCCCHHCHHHHCCCEECCCCHHHHHCCCCCEEEECCCCCCCHHHHHHH
AAILLGSLTGIDEKDVATHKSEGIAEHDYTKYLDVNGLHGAKIGVYSNAPKDYYETGEYD
HHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHEEECCCCCCCEEEECCCCCHHHHCCCCHH
EKLFKETIEVLRSEGATVVEGIDIPSFHREWSWGVSLYELKHSLDNYLSKLPSTMPVHSI
HHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCHHHH
SELMEFNENIAERALKYGQTKLERRKDFPNTLRNPEYLHARLEDIYFSQEQGIDFALKKY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHCCHHCCCEEEEEEE
NLDAILFPSYIGSTICAKAGYPSIAIPAGYMEDGRPFGITIASTAFSEGTLIKLAYAFEQ
CCCEEECHHHHHHHHHHCCCCCCEECCCCCCCCCCEEEEEEEECCCCCCCEEEEHHHHHH
ATKHREIPGLS
HHHHCCCCCCC
>Mature Secondary Structure
MKMQFNTLLQKELTIHDIQKAMEDEKLTSKELVMYYLHRVAKYDQDGPKINSILEINPDA
CCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCE
IFIAEALDHERKIKGVRGPLHGIPVLLKDNIETNDSMHTSAGTIALEQNISSEDAFLVTK
EEEEHHHCHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCCCHHHHEE
LREAGAVILGKTNMTELANAMSFEMWAGYSARGGQTINPYGTGDDDMIVGGSSTGSAIAV
HHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCEEEECCCCCCEEEE
ASNFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
ECCEEEEEECCCCCCCHHCHHHHCCCEECCCCHHHHHCCCCCEEEECCCCCCCHHHHHHH
AAILLGSLTGIDEKDVATHKSEGIAEHDYTKYLDVNGLHGAKIGVYSNAPKDYYETGEYD
HHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHEEECCCCCCCEEEECCCCCHHHHCCCCHH
EKLFKETIEVLRSEGATVVEGIDIPSFHREWSWGVSLYELKHSLDNYLSKLPSTMPVHSI
HHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCHHHH
SELMEFNENIAERALKYGQTKLERRKDFPNTLRNPEYLHARLEDIYFSQEQGIDFALKKY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHCCHHCCCEEEEEEE
NLDAILFPSYIGSTICAKAGYPSIAIPAGYMEDGRPFGITIASTAFSEGTLIKLAYAFEQ
CCCEEECHHHHHHHHHHCCCCCCEECCCCCCCCCCEEEEEEEECCCCCCCEEEEHHHHHH
ATKHREIPGLS
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Glu-tRNA(Gln); L-glutamine

Specific reaction: ATP + Glu-tRNA(Gln) + L-glutamine = ADP + phosphate + Gln-tRNA(Gln) + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA