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The map label for this gene is rrmA [C]

Identifier: 52143325

GI number: 52143325

Start: 2025414

End: 2026124

Strand: Direct

Name: rrmA [C]

Synonym: BCZK1913

Alternate gene names: 52143325

Gene position: 2025414-2026124 (Clockwise)

Preceding gene: 52143327

Following gene: 52143323

Centisome position: 38.21

GC content: 36.71

Gene sequence:

>711_bases
ATGACGAAATTTAATTGGCATGAATCAGCAGAAAAGAAATGGGATAGTAGTGCAGAGTTTTGGAATCAAAATAGTCAGGA
GATGTGGGACAGCGGGAGTAGGAGTACAATTATTCCGTTTTTTGAACAGTACGTAAAGAAAGAAGCCGAGGTGCTCGATG
TTGGTTGTGGTGATGGATACGGTACATATAAATTAAGTCGCACGGGATATAAAGCAGTTGGAGTGGATATATCAGAAGTG
ATGATTCAAAAAGGGAAGGAGCGCGGAGAAGGACCTGATTTATCTTTTATAAAAGGAGATCTTTCCTCCTTACCATTTGA
AAATGAACAATTTGAAGCAATTATGGCAATTAATTCTTTAGAATGGACCGAGGAGCCATTACGAGCATTAAATGAGATAA
AGCGTGTTCTAAAAAGTGATGGATATGCATGTATTGCAATTTTAGGACCGACAGCAAAGCCAAGAGAGAACAGTTATCCT
CGTTTATACGGCAAAGACGTTGTATGTAATACGATGATGCCTTGGGAATTTGAACAGTTAGTAAAAGAACAAGGTTTTAA
AGTTGTAGATGGTATCGGTGTATATAAGCGCGGAGTAAATGAGAAGATGTTAGGTCAACTATCTACAGATTTACAACAAT
CGTTAACGTTCTTATGGGTATTTATGTTAAAAAAGCATAAAGAAATGAAAGAATTTTTAGGAGGTAAATAA

Upstream 100 bases:

>100_bases
TCCTTCATTAGCATGTACATTTCCTAATGAAGATAAAACAGGAATTTTGGAAAATGATGTAGAATGAAATGAGTGATAAA
GCGTGAAGGTAGGGGAATGT

Downstream 100 bases:

>100_bases
GTGCAGAAAATACAAAAAACTGATACAATATCATCAGAACCAATTAAAGGGGGACTAGGGTATATGACAACTACTACAAC
AGTTAAATCCGATATTGAAA

Product: methyltransferase

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MTKFNWHESAEKKWDSSAEFWNQNSQEMWDSGSRSTIIPFFEQYVKKEAEVLDVGCGDGYGTYKLSRTGYKAVGVDISEV
MIQKGKERGEGPDLSFIKGDLSSLPFENEQFEAIMAINSLEWTEEPLRALNEIKRVLKSDGYACIAILGPTAKPRENSYP
RLYGKDVVCNTMMPWEFEQLVKEQGFKVVDGIGVYKRGVNEKMLGQLSTDLQQSLTFLWVFMLKKHKEMKEFLGGK

Sequences:

>Translated_236_residues
MTKFNWHESAEKKWDSSAEFWNQNSQEMWDSGSRSTIIPFFEQYVKKEAEVLDVGCGDGYGTYKLSRTGYKAVGVDISEV
MIQKGKERGEGPDLSFIKGDLSSLPFENEQFEAIMAINSLEWTEEPLRALNEIKRVLKSDGYACIAILGPTAKPRENSYP
RLYGKDVVCNTMMPWEFEQLVKEQGFKVVDGIGVYKRGVNEKMLGQLSTDLQQSLTFLWVFMLKKHKEMKEFLGGK
>Mature_235_residues
TKFNWHESAEKKWDSSAEFWNQNSQEMWDSGSRSTIIPFFEQYVKKEAEVLDVGCGDGYGTYKLSRTGYKAVGVDISEVM
IQKGKERGEGPDLSFIKGDLSSLPFENEQFEAIMAINSLEWTEEPLRALNEIKRVLKSDGYACIAILGPTAKPRENSYPR
LYGKDVVCNTMMPWEFEQLVKEQGFKVVDGIGVYKRGVNEKMLGQLSTDLQQSLTFLWVFMLKKHKEMKEFLGGK

Specific function: Catalyzes the methylation of the lipid moiety of the intermediate compounds phthiotriol and glycosylated phenolphthiotriol dimycoserosates to form phthiocerol dimycocerosates (DIM A) and glycosylated phenolphthiocerol dimycocerosates (PGL) [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Phthiotriol/phenolphthiotriol dimycocerosates methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 26986; Mature: 26855

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFNWHESAEKKWDSSAEFWNQNSQEMWDSGSRSTIIPFFEQYVKKEAEVLDVGCGDGY
CCCCCCCHHHHHHCCCCHHHHCCCCHHHHCCCCCCEEHHHHHHHHHHHHHEEECCCCCCC
GTYKLSRTGYKAVGVDISEVMIQKGKERGEGPDLSFIKGDLSSLPFENEQFEAIMAINSL
CCEEECCCCCEEECCCHHHHHHHHCCCCCCCCCHHHHHCHHHHCCCCCHHHHHHHHHHCC
EWTEEPLRALNEIKRVLKSDGYACIAILGPTAKPRENSYPRLYGKDVVCNTMMPWEFEQL
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCHHHHHH
VKEQGFKVVDGIGVYKRGVNEKMLGQLSTDLQQSLTFLWVFMLKKHKEMKEFLGGK
HHHCCCEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKFNWHESAEKKWDSSAEFWNQNSQEMWDSGSRSTIIPFFEQYVKKEAEVLDVGCGDGY
CCCCCCHHHHHHCCCCHHHHCCCCHHHHCCCCCCEEHHHHHHHHHHHHHEEECCCCCCC
GTYKLSRTGYKAVGVDISEVMIQKGKERGEGPDLSFIKGDLSSLPFENEQFEAIMAINSL
CCEEECCCCCEEECCCHHHHHHHHCCCCCCCCCHHHHHCHHHHCCCCCHHHHHHHHHHCC
EWTEEPLRALNEIKRVLKSDGYACIAILGPTAKPRENSYPRLYGKDVVCNTMMPWEFEQL
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCHHHHHH
VKEQGFKVVDGIGVYKRGVNEKMLGQLSTDLQQSLTFLWVFMLKKHKEMKEFLGGK
HHHCCCEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]