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The map label for this gene is acpD [H]

Identifier: 52143214

GI number: 52143214

Start: 2146746

End: 2147381

Strand: Direct

Name: acpD [H]

Synonym: BCZK2023

Alternate gene names: 52143214

Gene position: 2146746-2147381 (Clockwise)

Preceding gene: 52143215

Following gene: 52143213

Centisome position: 40.5

GC content: 37.74

Gene sequence:

>636_bases
ATGACAAAAGTACTATTCATTACAGCAAATCCAAATTCAGCAGAAGGTTCATTCGGAATGGCAGTAGGGGAAGCTTTCAT
CGAAGCTTATAAAAATGAACATCCACAAGACGAAGTTGTAACAATTGATTTATTCAACACTACAGTACCAGCAATCGATG
CAGATGTATTCGCTGCTTGGGGTAAATTTGCAGCAGGTGAAGGCTTTGAGACTTTAACTGAAGCTCAACAACAAAAAGTT
GCAGCAATGAACACAAACTTAGAAACATTTATGAATGCAGATCGTTATGTATTCGTAACTCCAATGTGGAACTTTAGCTA
CCCACCAGTAGTAAAAGCATACTTAGATAACGTAGCAATCGCAGGTAAAACATTCAAATATACTGAAAATGGTCCAGTTG
GCTTATTAGAAGGTAAAAAAGCACTTCACATTCAAGCGACAGGTGGCGTATATTCTGAAGGAGCATACGCAGCTGTAGAC
TTCGGACGCAATCACTTGAAAACTGTATTAGGATTTGTCGGTGTAAATGATACTGAATATATTGCAGTTGAAGGTATGAA
TGCAAACCCTGAAAAAGCACAAGAAATTAAAGAAGCAGCAATTGCTAATGCTCGTGAATTAGCAAAACGTTTCTAA

Upstream 100 bases:

>100_bases
GAACGTAACTTTTTATATTATACTTACATAAGGTAAGTTATTTACTTTCGAATTATAAATCTCAAATTAAAGATAAAATG
TAGAAAAGGGAGAGAATACA

Downstream 100 bases:

>100_bases
TATATCATACTTAAAAATGTCCAAACACTTGTTGTTTGGACATTTTTTCTTCTTTTTCCTTCGTTTGTCTAGTATAATGA
AGGTCGGAATGATAATAGGT

Product: azoreductase

Products: NAD+; NADP+; reduced acceptor

Alternate protein names: Azo-dye reductase 2; FMN-dependent NADH-azo compound oxidoreductase 2 [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTKVLFITANPNSAEGSFGMAVGEAFIEAYKNEHPQDEVVTIDLFNTTVPAIDADVFAAWGKFAAGEGFETLTEAQQQKV
AAMNTNLETFMNADRYVFVTPMWNFSYPPVVKAYLDNVAIAGKTFKYTENGPVGLLEGKKALHIQATGGVYSEGAYAAVD
FGRNHLKTVLGFVGVNDTEYIAVEGMNANPEKAQEIKEAAIANARELAKRF

Sequences:

>Translated_211_residues
MTKVLFITANPNSAEGSFGMAVGEAFIEAYKNEHPQDEVVTIDLFNTTVPAIDADVFAAWGKFAAGEGFETLTEAQQQKV
AAMNTNLETFMNADRYVFVTPMWNFSYPPVVKAYLDNVAIAGKTFKYTENGPVGLLEGKKALHIQATGGVYSEGAYAAVD
FGRNHLKTVLGFVGVNDTEYIAVEGMNANPEKAQEIKEAAIANARELAKRF
>Mature_210_residues
TKVLFITANPNSAEGSFGMAVGEAFIEAYKNEHPQDEVVTIDLFNTTVPAIDADVFAAWGKFAAGEGFETLTEAQQQKVA
AMNTNLETFMNADRYVFVTPMWNFSYPPVVKAYLDNVAIAGKTFKYTENGPVGLLEGKKALHIQATGGVYSEGAYAAVDF
GRNHLKTVLGFVGVNDTEYIAVEGMNANPEKAQEIKEAAIANARELAKRF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=204, Percent_Identity=34.8039215686275, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 1.6.99.2

Molecular weight: Translated: 22933; Mature: 22802

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVLFITANPNSAEGSFGMAVGEAFIEAYKNEHPQDEVVTIDLFNTTVPAIDADVFAAW
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHH
GKFAAGEGFETLTEAQQQKVAAMNTNLETFMNADRYVFVTPMWNFSYPPVVKAYLDNVAI
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCEEE
AGKTFKYTENGPVGLLEGKKALHIQATGGVYSEGAYAAVDFGRNHLKTVLGFVGVNDTEY
CCCEEEECCCCCEEEEECCEEEEEEECCCEECCCCEEEECCCHHHHHHHHHHHCCCCCCE
IAVEGMNANPEKAQEIKEAAIANARELAKRF
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKVLFITANPNSAEGSFGMAVGEAFIEAYKNEHPQDEVVTIDLFNTTVPAIDADVFAAW
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHH
GKFAAGEGFETLTEAQQQKVAAMNTNLETFMNADRYVFVTPMWNFSYPPVVKAYLDNVAI
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCEEE
AGKTFKYTENGPVGLLEGKKALHIQATGGVYSEGAYAAVDFGRNHLKTVLGFVGVNDTEY
CCCEEEECCCCCEEEEECCEEEEEEECCCEECCCCEEEECCCHHHHHHHHHHHCCCCCCE
IAVEGMNANPEKAQEIKEAAIANARELAKRF
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; NADPH; H+; acceptor

Specific reaction: NAD(P)H + H+ + acceptor = NAD(P)+ + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721630 [H]