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The map label for this gene is prsA [H]

Identifier: 52143135

GI number: 52143135

Start: 2220687

End: 2221538

Strand: Direct

Name: prsA [H]

Synonym: BCZK2101

Alternate gene names: 52143135

Gene position: 2220687-2221538 (Clockwise)

Preceding gene: 52143138

Following gene: 52143136

Centisome position: 41.89

GC content: 32.39

Gene sequence:

>852_bases
TTGAAAAAGAAAAAGCTATTTTTAGGAACAATTATTTCATGCGTAGTACTGGCATTATCTGCATGTGGTTCCTCAGACAA
CGTAGTAACATCAAAAGTAGGAAATATTACAGAGAAAGAATTAAGTAAAGAATTAAGACAAAAATATGGGGAAAGTACTT
TATACCAAATGGTGTTAAGTAAGGCGTTGCTAGATAAATATAAAGTTTCAGATGAGGAAGCAAAAAAACAAGTAGAAGAA
GCAAAAGATAAAATGGGTGACAACTTCAAATCGACTTTAGAGCAAGTTGGATTGAAAAACGAAGATGAATTAAAAGAAAA
AATGAAGCCAGAAATTGCATTTGAGAAAGCGATTAAAGCGACTGTTACAGAAAAAGATGTAAAAGATAACTATAAACCAG
AAATGAAGGTAAGTCACATTTTAATAAAAGATGAAAAGACTGCTAAAGAAGTGAAAGAGAAAGTAAGTAATGGTGAAGAC
TTTGCGGCTTTAGCGAAGCAATACTCAGAGGATACTGGTTCAAAGGAACAAGGCGGAGAAATAACTGGTTTTGCTCCAGG
GCAAACGGTGAAGGAATTTGAGGAAGCTGCATATAAATTAGATGCAGGACAAGTAAGTGAGCCAGTAAAAACAACTTATG
GTTACCATATTATTAAAGTGACAGATAAAAAAGAATTGAAGCCATATGATGAAGTAAAAGATCAAATTCGTAAAGATTTA
GAACAGCAAAGGCTGCAAGATACAACAGGCAAATGGAAACAGCAAGTAGTCAATGATTTATTGAAAGATGCTGATATTAA
AGTGAATGATAAAGACTTTAAAAATACATTTGAATTTCTAGAAAAGAAATAA

Upstream 100 bases:

>100_bases
GTTTTTTCAAATGAAATGAATTACATGTAGAAAACACATTGTTTACACAAGAACATTGTATAGTTATTCTTGTATAATAA
TAAAAGTGAGGTATTTTGAA

Downstream 100 bases:

>100_bases
TGACTGTAATTAGAAAAGGGAACCTTTAAAGGTTCCCTTTTCTAGTTAGTGTGTGAGTTATAAATAGATAGTAAGACAAT
ACAATATTACATATAAATAT

Product: peptidylprolyl isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKKKKLFLGTIISCVVLALSACGSSDNVVTSKVGNITEKELSKELRQKYGESTLYQMVLSKALLDKYKVSDEEAKKQVEE
AKDKMGDNFKSTLEQVGLKNEDELKEKMKPEIAFEKAIKATVTEKDVKDNYKPEMKVSHILIKDEKTAKEVKEKVSNGED
FAALAKQYSEDTGSKEQGGEITGFAPGQTVKEFEEAAYKLDAGQVSEPVKTTYGYHIIKVTDKKELKPYDEVKDQIRKDL
EQQRLQDTTGKWKQQVVNDLLKDADIKVNDKDFKNTFEFLEKK

Sequences:

>Translated_283_residues
MKKKKLFLGTIISCVVLALSACGSSDNVVTSKVGNITEKELSKELRQKYGESTLYQMVLSKALLDKYKVSDEEAKKQVEE
AKDKMGDNFKSTLEQVGLKNEDELKEKMKPEIAFEKAIKATVTEKDVKDNYKPEMKVSHILIKDEKTAKEVKEKVSNGED
FAALAKQYSEDTGSKEQGGEITGFAPGQTVKEFEEAAYKLDAGQVSEPVKTTYGYHIIKVTDKKELKPYDEVKDQIRKDL
EQQRLQDTTGKWKQQVVNDLLKDADIKVNDKDFKNTFEFLEKK
>Mature_283_residues
MKKKKLFLGTIISCVVLALSACGSSDNVVTSKVGNITEKELSKELRQKYGESTLYQMVLSKALLDKYKVSDEEAKKQVEE
AKDKMGDNFKSTLEQVGLKNEDELKEKMKPEIAFEKAIKATVTEKDVKDNYKPEMKVSHILIKDEKTAKEVKEKVSNGED
FAALAKQYSEDTGSKEQGGEITGFAPGQTVKEFEEAAYKLDAGQVSEPVKTTYGYHIIKVTDKKELKPYDEVKDQIRKDL
EQQRLQDTTGKWKQQVVNDLLKDADIKVNDKDFKNTFEFLEKK

Specific function: Plays a major role in protein secretion by helping the post-translocational extracellular folding of several secreted proteins. Important for the secretion of the protective antigen. The three psrA proteins in this organism show different but overlapping

COG id: COG0760

COG function: function code O; Parvulin-like peptidyl-prolyl isomerase

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PpiC domain [H]

Homologues:

Organism=Escherichia coli, GI1786238, Length=133, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790211, Length=82, Percent_Identity=40.2439024390244, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023059
- InterPro:   IPR000297
- InterPro:   IPR008880 [H]

Pfam domain/function: PF00639 Rotamase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 32099; Mature: 32099

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50198 PPIC_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKKLFLGTIISCVVLALSACGSSDNVVTSKVGNITEKELSKELRQKYGESTLYQMVLS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHH
KALLDKYKVSDEEAKKQVEEAKDKMGDNFKSTLEQVGLKNEDELKEKMKPEIAFEKAIKA
HHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHH
TVTEKDVKDNYKPEMKVSHILIKDEKTAKEVKEKVSNGEDFAALAKQYSEDTGSKEQGGE
HHHHHHHHCCCCCCHHHHHEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
ITGFAPGQTVKEFEEAAYKLDAGQVSEPVKTTYGYHIIKVTDKKELKPYDEVKDQIRKDL
EECCCCCHHHHHHHHHHHHCCCHHCCCCHHHHCCEEEEEECCCHHCCCHHHHHHHHHHHH
EQQRLQDTTGKWKQQVVNDLLKDADIKVNDKDFKNTFEFLEKK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKKKLFLGTIISCVVLALSACGSSDNVVTSKVGNITEKELSKELRQKYGESTLYQMVLS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHH
KALLDKYKVSDEEAKKQVEEAKDKMGDNFKSTLEQVGLKNEDELKEKMKPEIAFEKAIKA
HHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHH
TVTEKDVKDNYKPEMKVSHILIKDEKTAKEVKEKVSNGEDFAALAKQYSEDTGSKEQGGE
HHHHHHHHCCCCCCHHHHHEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
ITGFAPGQTVKEFEEAAYKLDAGQVSEPVKTTYGYHIIKVTDKKELKPYDEVKDQIRKDL
EECCCCCHHHHHHHHHHHHCCCHHCCCCHHHHCCEEEEEECCCHHCCCHHHHHHHHHHHH
EQQRLQDTTGKWKQQVVNDLLKDADIKVNDKDFKNTFEFLEKK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12721629; 12606539 [H]