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The map label for this gene is thrS [H]

Identifier: 52143093

GI number: 52143093

Start: 2273566

End: 2275485

Strand: Direct

Name: thrS [H]

Synonym: BCZK2145

Alternate gene names: 52143093

Gene position: 2273566-2275485 (Clockwise)

Preceding gene: 52143096

Following gene: 52143092

Centisome position: 42.89

GC content: 34.53

Gene sequence:

>1920_bases
ATGAAAGAACAGATGATTGAAATTAATTTTCCAGATGGTAGCGTGAAAGAATTTGTAAAAGGAATTACTTTAGAAGAAAT
TGCGGGATCCATTAGCAATAGTTTAAAAAAGAAAGCAGTCGCTGGGAAAGTAAATGATGAGTTATATGATTTACGCCGTA
ATATTGAAGAAAATGCGGAAGTAGAAATCATTACTATAGATTCAAATGAAGGAGTAGAAATTGCAAGACACTCCGCGGCA
CATATTTTAGCGCAAGCTGTAAAAAGATTGTATGGTGATATAAATCTTGGAGTAGGGCCTGTTATTGAAAATGGATTTTA
TTATGATATGGATCTTCTAAGTAGTGTAAACGTAGAAGATTTACGAAAAATCGAAAAAGAAATGAAAAATATTATAAAAG
AAAATGTAAAAATAGAACGAGTTGAGGTTTCTCGAGAAGAAGCGGCAAAACTATTTCAAGAAATGAATGATCGTTTGAAA
TTAGAACTTTTAGAAGCAATTCCTGATGAGGAAAGTGTAACACTATATAAGCAAGGTGAATTTGTAGATTTATGTAGAGG
GCCACATTTACCATCTACAGGTTATTTGAAAGCATTTCAATTAACTCACGTTTCCGGTGCATATTGGCGAGGAGATAGTA
ATAATCAAGTGCTTCAGCGTATATATGGTGTTGCATTCTCTTCCCAGAAAGCATTAGAAGAGTATTTACATTTCGTTGAA
GAAGCAGCCAAAAGAAACCATCGTAAGTTAGGCAATGAACTGGAGTTATTTATGTTTTCTGAAGAGGCTCCGGGAATGCC
GTTTTATTTACCGAAAGGCCAAATTATTCGTAATGAATTAGAAGCTTTTTTAAGAGAAATTCAAAAAGAATATAATTATC
AAGAAGTACGCACTCCATTCATGATGAACCAAGAAGTATGGGAAAGATCAGGGCACTGGGGACATTATAAAGATAATATG
TATTTCTCAGAAGTGGATAATAAAAGTTTTGCATTAAAACCAATGAACTGCCCAGGGCATATGCTAATGTTCAAAAATAA
ATTGCATTCTTATCGCGAATTACCGATTCGTATGTGTGAGTTTGGTCAAGTACATCGCCATGAATTTAGTGGGGCGTTAA
ACGGATTATTAAGAGTACGTACTTTCTGCCAAGATGATGCACATTTATTTGTAACTCCAGAACAAATTGAAGATGAAATC
AAATCTGTAATGGCGCAAATCGATTATGTATATAAAACTTTTGGATTTGAATATGAAGTAGAACTTTCTACTCGTCCAGA
AGATTCAATGGGTGATGATAAGTTATGGGAGCAAGCAGAAGCGTCATTAGAAAATGTATTACAATCGCTAAATTATAAAT
ATAGACTAAATGAAGGCGACGGTGCATTTTACGGACCGAAAATTGACTTCCATATTAAAGACGCTTTAAATAGAAGTCAT
CAGTGCGGAACAATTCAGCTTGATTTCCAAATGCCAGAGAAATTTGATTTAAATTATATAGATGAGAAGAATGAAAAGAG
AAGACCAGTTGTCATTCACAGGGCAGTATTAGGATCTTTAGATCGTTTCTTAGCAATATTAATTGAGCACTTTGGAGGTG
CATTCCCGGCATGGATAGCGCCAGTTCAAGTGAAAGTCATTCCAGTTTCGAATGCAGTCCATGTACAATACGCAGATGAG
GTTGCAGATAAATTAGCACAAGCTGGTGTTCGTGTCGAACGAGATATGCGAGATGAAAAATTAGGATATAAAATAAGAGA
AGCACAAATGCAAAAAGTTCCTTATGTTCTTGTTATTGGTGATAAGGAAATGGAAAACGGAGCTGTAAATGTGCGTAAAT
ATGGGGAAGAAAAATCTGATGTAATTGAACTAGATATGTTTGTGGAAAGTATAAAAGAGGAAATAAATAATCGAAAATAG

Upstream 100 bases:

>100_bases
ATGTTTGTGATGGGAATCTGGGTGGAACCACGGGTATAAGCACGCTCGTCCCTATTGTAGGGATGAGTGTGCTTTTTATT
ATGAAATGGAGAGATGAAAA

Downstream 100 bases:

>100_bases
ATTTTAAAAAGAAAACACATCAATTCAAAAATGGTGTGTTTTCTTTTTTACCTTACAAAAATGTAAGATAAATGTAAGCG
AAATCGAAGGAACGAGTTGT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MKEQMIEINFPDGSVKEFVKGITLEEIAGSISNSLKKKAVAGKVNDELYDLRRNIEENAEVEIITIDSNEGVEIARHSAA
HILAQAVKRLYGDINLGVGPVIENGFYYDMDLLSSVNVEDLRKIEKEMKNIIKENVKIERVEVSREEAAKLFQEMNDRLK
LELLEAIPDEESVTLYKQGEFVDLCRGPHLPSTGYLKAFQLTHVSGAYWRGDSNNQVLQRIYGVAFSSQKALEEYLHFVE
EAAKRNHRKLGNELELFMFSEEAPGMPFYLPKGQIIRNELEAFLREIQKEYNYQEVRTPFMMNQEVWERSGHWGHYKDNM
YFSEVDNKSFALKPMNCPGHMLMFKNKLHSYRELPIRMCEFGQVHRHEFSGALNGLLRVRTFCQDDAHLFVTPEQIEDEI
KSVMAQIDYVYKTFGFEYEVELSTRPEDSMGDDKLWEQAEASLENVLQSLNYKYRLNEGDGAFYGPKIDFHIKDALNRSH
QCGTIQLDFQMPEKFDLNYIDEKNEKRRPVVIHRAVLGSLDRFLAILIEHFGGAFPAWIAPVQVKVIPVSNAVHVQYADE
VADKLAQAGVRVERDMRDEKLGYKIREAQMQKVPYVLVIGDKEMENGAVNVRKYGEEKSDVIELDMFVESIKEEINNRK

Sequences:

>Translated_639_residues
MKEQMIEINFPDGSVKEFVKGITLEEIAGSISNSLKKKAVAGKVNDELYDLRRNIEENAEVEIITIDSNEGVEIARHSAA
HILAQAVKRLYGDINLGVGPVIENGFYYDMDLLSSVNVEDLRKIEKEMKNIIKENVKIERVEVSREEAAKLFQEMNDRLK
LELLEAIPDEESVTLYKQGEFVDLCRGPHLPSTGYLKAFQLTHVSGAYWRGDSNNQVLQRIYGVAFSSQKALEEYLHFVE
EAAKRNHRKLGNELELFMFSEEAPGMPFYLPKGQIIRNELEAFLREIQKEYNYQEVRTPFMMNQEVWERSGHWGHYKDNM
YFSEVDNKSFALKPMNCPGHMLMFKNKLHSYRELPIRMCEFGQVHRHEFSGALNGLLRVRTFCQDDAHLFVTPEQIEDEI
KSVMAQIDYVYKTFGFEYEVELSTRPEDSMGDDKLWEQAEASLENVLQSLNYKYRLNEGDGAFYGPKIDFHIKDALNRSH
QCGTIQLDFQMPEKFDLNYIDEKNEKRRPVVIHRAVLGSLDRFLAILIEHFGGAFPAWIAPVQVKVIPVSNAVHVQYADE
VADKLAQAGVRVERDMRDEKLGYKIREAQMQKVPYVLVIGDKEMENGAVNVRKYGEEKSDVIELDMFVESIKEEINNRK
>Mature_639_residues
MKEQMIEINFPDGSVKEFVKGITLEEIAGSISNSLKKKAVAGKVNDELYDLRRNIEENAEVEIITIDSNEGVEIARHSAA
HILAQAVKRLYGDINLGVGPVIENGFYYDMDLLSSVNVEDLRKIEKEMKNIIKENVKIERVEVSREEAAKLFQEMNDRLK
LELLEAIPDEESVTLYKQGEFVDLCRGPHLPSTGYLKAFQLTHVSGAYWRGDSNNQVLQRIYGVAFSSQKALEEYLHFVE
EAAKRNHRKLGNELELFMFSEEAPGMPFYLPKGQIIRNELEAFLREIQKEYNYQEVRTPFMMNQEVWERSGHWGHYKDNM
YFSEVDNKSFALKPMNCPGHMLMFKNKLHSYRELPIRMCEFGQVHRHEFSGALNGLLRVRTFCQDDAHLFVTPEQIEDEI
KSVMAQIDYVYKTFGFEYEVELSTRPEDSMGDDKLWEQAEASLENVLQSLNYKYRLNEGDGAFYGPKIDFHIKDALNRSH
QCGTIQLDFQMPEKFDLNYIDEKNEKRRPVVIHRAVLGSLDRFLAILIEHFGGAFPAWIAPVQVKVIPVSNAVHVQYADE
VADKLAQAGVRVERDMRDEKLGYKIREAQMQKVPYVLVIGDKEMENGAVNVRKYGEEKSDVIELDMFVESIKEEINNRK

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=611, Percent_Identity=45.4991816693944, Blast_Score=555, Evalue=1e-158,
Organism=Homo sapiens, GI38570111, Length=637, Percent_Identity=43.6420722135008, Blast_Score=555, Evalue=1e-158,
Organism=Homo sapiens, GI20070344, Length=656, Percent_Identity=39.4817073170732, Blast_Score=481, Evalue=1e-136,
Organism=Escherichia coli, GI1788013, Length=636, Percent_Identity=46.5408805031447, Blast_Score=630, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71984192, Length=631, Percent_Identity=44.5324881141046, Blast_Score=558, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI71984184, Length=631, Percent_Identity=44.5324881141046, Blast_Score=558, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6322112, Length=627, Percent_Identity=44.1786283891547, Blast_Score=549, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6322654, Length=396, Percent_Identity=41.1616161616162, Blast_Score=320, Evalue=5e-88,
Organism=Drosophila melanogaster, GI24583839, Length=642, Percent_Identity=44.2367601246106, Blast_Score=561, Evalue=1e-160,
Organism=Drosophila melanogaster, GI24583842, Length=642, Percent_Identity=44.2367601246106, Blast_Score=560, Evalue=1e-160,
Organism=Drosophila melanogaster, GI28574141, Length=642, Percent_Identity=44.2367601246106, Blast_Score=560, Evalue=1e-160,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73845; Mature: 73845

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEQMIEINFPDGSVKEFVKGITLEEIAGSISNSLKKKAVAGKVNDELYDLRRNIEENAE
CCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
VEIITIDSNEGVEIARHSAAHILAQAVKRLYGDINLGVGPVIENGFYYDMDLLSSVNVED
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECHHHHHCCCHHH
LRKIEKEMKNIIKENVKIERVEVSREEAAKLFQEMNDRLKLELLEAIPDEESVTLYKQGE
HHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
FVDLCRGPHLPSTGYLKAFQLTHVSGAYWRGDSNNQVLQRIYGVAFSSQKALEEYLHFVE
EEEHHCCCCCCCCCCEEEEEEEECCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
EAAKRNHRKLGNELELFMFSEEAPGMPFYLPKGQIIRNELEAFLREIQKEYNYQEVRTPF
HHHHHHHHHCCCCEEEEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHHCCCH
MMNQEVWERSGHWGHYKDNMYFSEVDNKSFALKPMNCPGHMLMFKNKLHSYRELPIRMCE
HCCHHHHHCCCCCCCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHH
FGQVHRHEFSGALNGLLRVRTFCQDDAHLFVTPEQIEDEIKSVMAQIDYVYKTFGFEYEV
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ELSTRPEDSMGDDKLWEQAEASLENVLQSLNYKYRLNEGDGAFYGPKIDFHIKDALNRSH
EECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCEEEEEEHHHCCCCC
QCGTIQLDFQMPEKFDLNYIDEKNEKRRPVVIHRAVLGSLDRFLAILIEHFGGAFPAWIA
CCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHCC
PVQVKVIPVSNAVHVQYADEVADKLAQAGVRVERDMRDEKLGYKIREAQMQKVPYVLVIG
CEEEEEEECCCEEEEEEHHHHHHHHHHCCCHHHHHCCHHHHCCHHHHHHHHHCCEEEEEC
DKEMENGAVNVRKYGEEKSDVIELDMFVESIKEEINNRK
CCCCCCCCEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKEQMIEINFPDGSVKEFVKGITLEEIAGSISNSLKKKAVAGKVNDELYDLRRNIEENAE
CCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
VEIITIDSNEGVEIARHSAAHILAQAVKRLYGDINLGVGPVIENGFYYDMDLLSSVNVED
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECHHHHHCCCHHH
LRKIEKEMKNIIKENVKIERVEVSREEAAKLFQEMNDRLKLELLEAIPDEESVTLYKQGE
HHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
FVDLCRGPHLPSTGYLKAFQLTHVSGAYWRGDSNNQVLQRIYGVAFSSQKALEEYLHFVE
EEEHHCCCCCCCCCCEEEEEEEECCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
EAAKRNHRKLGNELELFMFSEEAPGMPFYLPKGQIIRNELEAFLREIQKEYNYQEVRTPF
HHHHHHHHHCCCCEEEEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHHCCCH
MMNQEVWERSGHWGHYKDNMYFSEVDNKSFALKPMNCPGHMLMFKNKLHSYRELPIRMCE
HCCHHHHHCCCCCCCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHH
FGQVHRHEFSGALNGLLRVRTFCQDDAHLFVTPEQIEDEIKSVMAQIDYVYKTFGFEYEV
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ELSTRPEDSMGDDKLWEQAEASLENVLQSLNYKYRLNEGDGAFYGPKIDFHIKDALNRSH
EECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCEEEEEEHHHCCCCC
QCGTIQLDFQMPEKFDLNYIDEKNEKRRPVVIHRAVLGSLDRFLAILIEHFGGAFPAWIA
CCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHCC
PVQVKVIPVSNAVHVQYADEVADKLAQAGVRVERDMRDEKLGYKIREAQMQKVPYVLVIG
CEEEEEEECCCEEEEEEHHHHHHHHHHCCCHHHHHCCHHHHCCHHHHHHHHHCCEEEEEC
DKEMENGAVNVRKYGEEKSDVIELDMFVESIKEEINNRK
CCCCCCCCEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2115870; 9384377; 9353933; 1379177 [H]