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The map label for this gene is ydaP [H]

Identifier: 52143085

GI number: 52143085

Start: 2283150

End: 2284850

Strand: Reverse

Name: ydaP [H]

Synonym: BCZK2152

Alternate gene names: 52143085

Gene position: 2284850-2283150 (Counterclockwise)

Preceding gene: 52143086

Following gene: 52143097

Centisome position: 43.1

GC content: 38.57

Gene sequence:

>1701_bases
ATGTTTGGGAAAACAGCCGGTGAAGCACTAGTTGACCTATTAATTGACTGGGGCGTAGAACACATTTACGGAATGCCTGG
AGATTCTATCAATTCTATAATTGAAGCTTTAAGAAAAAAACAAGATAAAATCAAATTTATTCAAGTACGCCATGAAGAAG
CTGGCGCACTAGCAGCAGCTGCCTATGCTAAGTTAACAGGAAAACTCGGCGTTTGCATGGCAATCGCAGGGCCCGGTGCT
ATACATTTATTAAATGGTTTATATGATGCGAAACTCGATAAAGCCCCTGTTCTAGCAATTTCAGGTCAAGTAGAAACCGA
TTTACTCGGTACGGATTTTTTCCAAGAAGTAAACTTAGAAAGATTGTTCGATGATGTCGCGGTTTATAACCAGCGCATTA
TGTCGGCAGAACAGCTACCTGCTGTCGTGAATCAAGCAATTCGAATGGCTTATACGAAAAAAGGTGTATCTGTTCTTACA
ATTCCGGATGATGTTCCGAAATTCGAAGTAAAGAATGGTGCTCGTGTTACAAATACTTCTTTTACATTACCTGAGTTATT
TCCGCAAGAAGAAGATTTACATGCTGCCATACTCGCAATAAATGAAGCGAAAAAGCCTGTTATTTTAGCTGGAAAAGGTG
CGAAACATGCAAGAGAATCTTTACTTACATTTGCAGAACACATTGGTGCTCCAATCGTACTTACATTACCAGCTAAAGGG
ATTATCCCTGATGAACATCCACTTTGTATCGGTGGGTTAGGATTAATTGGAACGAAACCTTCTTATGATGCAATGAAACA
TGCAGATACTTTAATTATGGTTGGTACTTCCTATCCTTTCACCGGTTTCTTACCTGAAAAAGCAAAAACAATTCATATCG
ATACTGATCCTGCCCAAATCGGAAAACGGTATGTAACAGATATCGGCTTAGCTGGTGATGCTGATAAAACATTAAAATGG
TTAATTGAGAATGTAGAAAAACATGAGGACCACTCCTTCTTAGAGCATCATCAAGAAATGATGAAAAAATGGGAAGAAAA
ATTACACAATCAAGAAGACGATTCTTCTACTCCTATTAAGCCACAACGCGTTATGCATGCACTGCAACATGTAGCTCATG
ATGACGCAATATTATCAGTGGATGTCGGAAATGTAACAGTATGGACAGCAAGGCACTTCCGTATGACAAATCAACAATTT
ATCATTTCTAGCTGGCTTGCAACTCTTGGCTGCGGTTTACCTGGTGCACTTGCTGGAAAAATTGCTTTCCCGGATAAACA
AGTATTTGCGGTTTGTGGAGACGGGGGTTTTGGAATGACAATGAACGATTTCGTAACAGCTGTTAAATATAAACTACCAA
TCATTGTCGTCGTTTTAAACAATAATAAAATTGCCATGATTAAATTCGAACAAGAAGTTATGGGTAATATTGAATTTGGT
ACAGACTTAACAAATCCTGATTTTGCAAAATACGCAGAGGCGTGCGGAGGAATTGGATACCGTGTTGAGAATGTAGATGA
ACTACTTCCTGCTTTCGAAAGTGCGGTAAAACAAAATAGTCCGTGTATTATTGATGTAGTTGTAGATGTAAATGAAGCAC
CAATGCCAGCAAAAATCACCTTTGGTCAAGCCGCTGGTTATACGAAGCATATGATTAAAGAATTATTTGAAGAAGGTAAG
ATTGATTTACCACCACTTTAA

Upstream 100 bases:

>100_bases
TTCGCTGGTCATCATTAAAGATAATAATATTATCATTTGTATATTTTTCCTTTTTCTCCAAAACATAATAGTATCTACCT
AATTATAAGGAGGAAAAATT

Downstream 100 bases:

>100_bases
ACGAAAAAAGATAGCTGAAAACGATTTGTCTTCAGCTATCTTTTTTTATTTTTGTATCTCTCTATAAAAAGAAAAGTCAT
TCGTAGTTGGTTGTACTTGT

Product: pyruvate oxidase

Products: acetyl phosphate; CO2; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MFGKTAGEALVDLLIDWGVEHIYGMPGDSINSIIEALRKKQDKIKFIQVRHEEAGALAAAAYAKLTGKLGVCMAIAGPGA
IHLLNGLYDAKLDKAPVLAISGQVETDLLGTDFFQEVNLERLFDDVAVYNQRIMSAEQLPAVVNQAIRMAYTKKGVSVLT
IPDDVPKFEVKNGARVTNTSFTLPELFPQEEDLHAAILAINEAKKPVILAGKGAKHARESLLTFAEHIGAPIVLTLPAKG
IIPDEHPLCIGGLGLIGTKPSYDAMKHADTLIMVGTSYPFTGFLPEKAKTIHIDTDPAQIGKRYVTDIGLAGDADKTLKW
LIENVEKHEDHSFLEHHQEMMKKWEEKLHNQEDDSSTPIKPQRVMHALQHVAHDDAILSVDVGNVTVWTARHFRMTNQQF
IISSWLATLGCGLPGALAGKIAFPDKQVFAVCGDGGFGMTMNDFVTAVKYKLPIIVVVLNNNKIAMIKFEQEVMGNIEFG
TDLTNPDFAKYAEACGGIGYRVENVDELLPAFESAVKQNSPCIIDVVVDVNEAPMPAKITFGQAAGYTKHMIKELFEEGK
IDLPPL

Sequences:

>Translated_566_residues
MFGKTAGEALVDLLIDWGVEHIYGMPGDSINSIIEALRKKQDKIKFIQVRHEEAGALAAAAYAKLTGKLGVCMAIAGPGA
IHLLNGLYDAKLDKAPVLAISGQVETDLLGTDFFQEVNLERLFDDVAVYNQRIMSAEQLPAVVNQAIRMAYTKKGVSVLT
IPDDVPKFEVKNGARVTNTSFTLPELFPQEEDLHAAILAINEAKKPVILAGKGAKHARESLLTFAEHIGAPIVLTLPAKG
IIPDEHPLCIGGLGLIGTKPSYDAMKHADTLIMVGTSYPFTGFLPEKAKTIHIDTDPAQIGKRYVTDIGLAGDADKTLKW
LIENVEKHEDHSFLEHHQEMMKKWEEKLHNQEDDSSTPIKPQRVMHALQHVAHDDAILSVDVGNVTVWTARHFRMTNQQF
IISSWLATLGCGLPGALAGKIAFPDKQVFAVCGDGGFGMTMNDFVTAVKYKLPIIVVVLNNNKIAMIKFEQEVMGNIEFG
TDLTNPDFAKYAEACGGIGYRVENVDELLPAFESAVKQNSPCIIDVVVDVNEAPMPAKITFGQAAGYTKHMIKELFEEGK
IDLPPL
>Mature_566_residues
MFGKTAGEALVDLLIDWGVEHIYGMPGDSINSIIEALRKKQDKIKFIQVRHEEAGALAAAAYAKLTGKLGVCMAIAGPGA
IHLLNGLYDAKLDKAPVLAISGQVETDLLGTDFFQEVNLERLFDDVAVYNQRIMSAEQLPAVVNQAIRMAYTKKGVSVLT
IPDDVPKFEVKNGARVTNTSFTLPELFPQEEDLHAAILAINEAKKPVILAGKGAKHARESLLTFAEHIGAPIVLTLPAKG
IIPDEHPLCIGGLGLIGTKPSYDAMKHADTLIMVGTSYPFTGFLPEKAKTIHIDTDPAQIGKRYVTDIGLAGDADKTLKW
LIENVEKHEDHSFLEHHQEMMKKWEEKLHNQEDDSSTPIKPQRVMHALQHVAHDDAILSVDVGNVTVWTARHFRMTNQQF
IISSWLATLGCGLPGALAGKIAFPDKQVFAVCGDGGFGMTMNDFVTAVKYKLPIIVVVLNNNKIAMIKFEQEVMGNIEFG
TDLTNPDFAKYAEACGGIGYRVENVDELLPAFESAVKQNSPCIIDVVVDVNEAPMPAKITFGQAAGYTKHMIKELFEEGK
IDLPPL

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=552, Percent_Identity=27.1739130434783, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI21361361, Length=584, Percent_Identity=23.2876712328767, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI1787096, Length=554, Percent_Identity=35.5595667870036, Blast_Score=356, Evalue=3e-99,
Organism=Escherichia coli, GI87081685, Length=570, Percent_Identity=30.3508771929825, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1790104, Length=546, Percent_Identity=29.6703296703297, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1786717, Length=556, Percent_Identity=26.4388489208633, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1788716, Length=554, Percent_Identity=25.2707581227437, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17531299, Length=548, Percent_Identity=25, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=548, Percent_Identity=25, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=568, Percent_Identity=25.5281690140845, Blast_Score=136, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=560, Percent_Identity=29.2857142857143, Blast_Score=225, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6320816, Length=558, Percent_Identity=24.0143369175627, Blast_Score=130, Evalue=6e-31,
Organism=Drosophila melanogaster, GI19922626, Length=571, Percent_Identity=26.2697022767075, Blast_Score=172, Evalue=8e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.3.3

Molecular weight: Translated: 61837; Mature: 61837

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGKTAGEALVDLLIDWGVEHIYGMPGDSINSIIEALRKKQDKIKFIQVRHEEAGALAAA
CCCCHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEECHHHCHHHHH
AYAKLTGKLGVCMAIAGPGAIHLLNGLYDAKLDKAPVLAISGQVETDLLGTDFFQEVNLE
HHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHCHHHHHHCCHH
RLFDDVAVYNQRIMSAEQLPAVVNQAIRMAYTKKGVSVLTIPDDVPKFEVKNGARVTNTS
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCEEECCC
FTLPELFPQEEDLHAAILAINEAKKPVILAGKGAKHARESLLTFAEHIGAPIVLTLPAKG
CCCHHHCCCCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCC
IIPDEHPLCIGGLGLIGTKPSYDAMKHADTLIMVGTSYPFTGFLPEKAKTIHIDTDPAQI
CCCCCCCEEEECCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHH
GKRYVTDIGLAGDADKTLKWLIENVEKHEDHSFLEHHQEMMKKWEEKLHNQEDDSSTPIK
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PQRVMHALQHVAHDDAILSVDVGNVTVWTARHFRMTNQQFIISSWLATLGCGLPGALAGK
HHHHHHHHHHHCCCCCEEEEECCCEEEEEEEEEEECCCHHHHHHHHHHHCCCCCCHHCCC
IAFPDKQVFAVCGDGGFGMTMNDFVTAVKYKLPIIVVVLNNNKIAMIKFEQEVMGNIEFG
EECCCCEEEEEECCCCCCCCHHHHHHHHEEECCEEEEEECCCEEEEEEEHHHHCCCCCCC
TDLTNPDFAKYAEACGGIGYRVENVDELLPAFESAVKQNSPCIIDVVVDVNEAPMPAKIT
CCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEE
FGQAAGYTKHMIKELFEEGKIDLPPL
CCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MFGKTAGEALVDLLIDWGVEHIYGMPGDSINSIIEALRKKQDKIKFIQVRHEEAGALAAA
CCCCHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEECHHHCHHHHH
AYAKLTGKLGVCMAIAGPGAIHLLNGLYDAKLDKAPVLAISGQVETDLLGTDFFQEVNLE
HHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHCHHHHHHCCHH
RLFDDVAVYNQRIMSAEQLPAVVNQAIRMAYTKKGVSVLTIPDDVPKFEVKNGARVTNTS
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCEEECCC
FTLPELFPQEEDLHAAILAINEAKKPVILAGKGAKHARESLLTFAEHIGAPIVLTLPAKG
CCCHHHCCCCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCC
IIPDEHPLCIGGLGLIGTKPSYDAMKHADTLIMVGTSYPFTGFLPEKAKTIHIDTDPAQI
CCCCCCCEEEECCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHH
GKRYVTDIGLAGDADKTLKWLIENVEKHEDHSFLEHHQEMMKKWEEKLHNQEDDSSTPIK
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PQRVMHALQHVAHDDAILSVDVGNVTVWTARHFRMTNQQFIISSWLATLGCGLPGALAGK
HHHHHHHHHHHCCCCCEEEEECCCEEEEEEEEEEECCCHHHHHHHHHHHCCCCCCHHCCC
IAFPDKQVFAVCGDGGFGMTMNDFVTAVKYKLPIIVVVLNNNKIAMIKFEQEVMGNIEFG
EECCCCEEEEEECCCCCCCCHHHHHHHHEEECCEEEEEECCCEEEEEEEHHHHCCCCCCC
TDLTNPDFAKYAEACGGIGYRVENVDELLPAFESAVKQNSPCIIDVVVDVNEAPMPAKIT
CCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEE
FGQAAGYTKHMIKELFEEGKIDLPPL
CCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; phosphate; O2; H2O

Specific reaction: pyruvate + phosphate + O2 + H2O = acetyl phosphate + CO2 + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]