Click here to switch to the map view.

The map label for this gene is cwlJ [H]

Identifier: 52142898

GI number: 52142898

Start: 2473467

End: 2473895

Strand: Reverse

Name: cwlJ [H]

Synonym: BCZK2341

Alternate gene names: 52142898

Gene position: 2473895-2473467 (Counterclockwise)

Preceding gene: 52142894

Following gene: 52142900

Centisome position: 46.67

GC content: 43.36

Gene sequence:

>429_bases
ATGCCCCTTATTCCATATAACGAAAGCGATGTTGATTTACTCGCTCGATTAATACGTGCTGAAGCTGAAGGCGAAGGTCG
ACAAGGTGAACAACTTGTTGGCGGCGTAGTAGTAAACCGTGTATTTTGTGATTGCTTAGATTTTAAACAACTTCGTTCTG
TACGTGATGCTGTATATCAAAGCCCCGGTGGATTCGAGGCAGTGCAATACGGATATTTTTATCAGCGTGCTCGTGAATCA
GAAAAAGAAATCGCACGAAAAGTTTTACAAGGTGAGTGGCGCTGGCCAGCAAGATGGGCACTTTGGTATTTCCGTCCAGT
TGCAGCTTGTCCGCCCGAATGGTATAACCAGCCATTTGTAGGACAGTTTAAAAGTCATTGTTTTTATGAACCTAGTGGTA
ATGAATGTCCGAAATTGTATTCACGGTAG

Upstream 100 bases:

>100_bases
ACAAGCTCCTCTTGAAATGTTAATTTTATGTAAAAAAGCACCAAGCTTTTTTAAAAACATACACTCTTTACTATAACAGA
AACAGAAAGGTGGTATCCCT

Downstream 100 bases:

>100_bases
TAAAATTTGCAACGCAAGTACCTAGAAAATTGGGTACTTGCGTTGCTTTTAACTTTGTTTTAGAAATCTAATAACGCGAT
GCTTTTTTCTATACCTACCA

Product: cell wall hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 141

Protein sequence:

>142_residues
MPLIPYNESDVDLLARLIRAEAEGEGRQGEQLVGGVVVNRVFCDCLDFKQLRSVRDAVYQSPGGFEAVQYGYFYQRARES
EKEIARKVLQGEWRWPARWALWYFRPVAACPPEWYNQPFVGQFKSHCFYEPSGNECPKLYSR

Sequences:

>Translated_142_residues
MPLIPYNESDVDLLARLIRAEAEGEGRQGEQLVGGVVVNRVFCDCLDFKQLRSVRDAVYQSPGGFEAVQYGYFYQRARES
EKEIARKVLQGEWRWPARWALWYFRPVAACPPEWYNQPFVGQFKSHCFYEPSGNECPKLYSR
>Mature_141_residues
PLIPYNESDVDLLARLIRAEAEGEGRQGEQLVGGVVVNRVFCDCLDFKQLRSVRDAVYQSPGGFEAVQYGYFYQRARESE
KEIARKVLQGEWRWPARWALWYFRPVAACPPEWYNQPFVGQFKSHCFYEPSGNECPKLYSR

Specific function: Probable spore cortex-lytic enzyme involved in the depolymerization of cortex peptidoglycan during germination [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CwlJ family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105 [H]

Pfam domain/function: PF07486 Hydrolase_2 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 16565; Mature: 16434

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLIPYNESDVDLLARLIRAEAEGEGRQGEQLVGGVVVNRVFCDCLDFKQLRSVRDAVYQ
CCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHC
SPGGFEAVQYGYFYQRARESEKEIARKVLQGEWRWPARWALWYFRPVAACPPEWYNQPFV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCC
GQFKSHCFYEPSGNECPKLYSR
HHHHHCEEECCCCCCCCCCCCC
>Mature Secondary Structure 
PLIPYNESDVDLLARLIRAEAEGEGRQGEQLVGGVVVNRVFCDCLDFKQLRSVRDAVYQ
CCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHC
SPGGFEAVQYGYFYQRARESEKEIARKVLQGEWRWPARWALWYFRPVAACPPEWYNQPFV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCC
GQFKSHCFYEPSGNECPKLYSR
HHHHHCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377; 9515903; 11466292; 11807087; 12177332 [H]