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The map label for this gene is acoA [H]

Identifier: 52142736

GI number: 52142736

Start: 2636477

End: 2637475

Strand: Reverse

Name: acoA [H]

Synonym: BCZK2505

Alternate gene names: 52142736

Gene position: 2637475-2636477 (Counterclockwise)

Preceding gene: 52142733

Following gene: 52142735

Centisome position: 49.76

GC content: 38.54

Gene sequence:

>999_bases
ATGTTAAAAACAACTGAAAGTAAAGGGAATGGAATTACGAAAGAACAAGCTCGCTGGATGTACGAAAAAATGTTAGAGAT
TCGTAAGTTTGAAGATAAGGTGCATGAATTATTCGCGCAAGGCGTTCTGCCAGGTTTCGTTCATTTATACGCTGGGGAAG
AAGCGGTAGCAGTTGGTGTATGTGCTCACTTAACGGATAGTGACAGTATTACAAGTACGCATAGAGGCCATGGGCATTGT
ATCGCAAAAGGTTGTGATTTAAACGGTATGATGGCTGAACTTTTCGGGAAAGCGACAGGATTATGTAAAGGGAAAGGCGG
ATCCATGCATATTGCTGATTTAGATAAAGGAATGCTTGGTGCAAATGGTATTGTAGGCGGTGGTTTCCCGCTTGCTTGCG
GCTCGGCATTAACAGCTAAATATAAAGGAACAAAAGATGTAAGTGTTTGTTTCTTCGGTGACGGAGCAAATAATGAAGGT
ACGTTTCATGAAGGGGTTAACTTAGCGGCGATTTGGAAGTTACCAGTTATTTTTATCGCTGAAAATAACGGTTACGGTGA
AGCAACGACATTTGAATATGCTTCAAGTTGTGATTCTATTGCGGATCGTGCAAAAGCTTATAACATTCCAGGTGTTCAAG
TGGATGGAAAAGATCTGCTAGCTGTATATAAAGCTGCTGAAGAAGCAGTAGAACGTGCGCGTAATGGCGATGGTCCAACA
ATAATTGAATGTATGACATATCGTAATTACGGTCATTTCGAAGGTGAAGCACAAACGTATAAAACTTCAGAAGAAAAAGA
AGAACATTTAAACGAAAAAGATGCAATTGTGAATTTCCGTAAGCATTTAATTCATGAAGCTTTATTAACTGAGTCTGAAC
TTGTGGATATGGAAAAGGCAGTAGATGATGCAGTACAAAAATCAATTGAATTTAGTGAAAATAGTCCATATCCAGAGGAT
GAAGAATTATTAAAAGATGTATACGTTTCTTACAAATAA

Upstream 100 bases:

>100_bases
TTTATTTTCAACATAGAGAGAAAACCTATTAAATCAAGGATTAGAATTTGGCACGGTACTTGCAATATAAAAGATGAACA
AGAAAATAGGAGGGCTGAAC

Downstream 100 bases:

>100_bases
AAGGAGGGTGAAACAGAATGACTAGAACAGTAAGTATGTCAACTGCAATTAATGAAGCGATGAAAATCTCAATGCGCCGT
GATGAAAATGTAATTTTAAT

Product: acetoin dehydrogenase (TPP-dependent) E1 component alpha subunit

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MLKTTESKGNGITKEQARWMYEKMLEIRKFEDKVHELFAQGVLPGFVHLYAGEEAVAVGVCAHLTDSDSITSTHRGHGHC
IAKGCDLNGMMAELFGKATGLCKGKGGSMHIADLDKGMLGANGIVGGGFPLACGSALTAKYKGTKDVSVCFFGDGANNEG
TFHEGVNLAAIWKLPVIFIAENNGYGEATTFEYASSCDSIADRAKAYNIPGVQVDGKDLLAVYKAAEEAVERARNGDGPT
IIECMTYRNYGHFEGEAQTYKTSEEKEEHLNEKDAIVNFRKHLIHEALLTESELVDMEKAVDDAVQKSIEFSENSPYPED
EELLKDVYVSYK

Sequences:

>Translated_332_residues
MLKTTESKGNGITKEQARWMYEKMLEIRKFEDKVHELFAQGVLPGFVHLYAGEEAVAVGVCAHLTDSDSITSTHRGHGHC
IAKGCDLNGMMAELFGKATGLCKGKGGSMHIADLDKGMLGANGIVGGGFPLACGSALTAKYKGTKDVSVCFFGDGANNEG
TFHEGVNLAAIWKLPVIFIAENNGYGEATTFEYASSCDSIADRAKAYNIPGVQVDGKDLLAVYKAAEEAVERARNGDGPT
IIECMTYRNYGHFEGEAQTYKTSEEKEEHLNEKDAIVNFRKHLIHEALLTESELVDMEKAVDDAVQKSIEFSENSPYPED
EELLKDVYVSYK
>Mature_332_residues
MLKTTESKGNGITKEQARWMYEKMLEIRKFEDKVHELFAQGVLPGFVHLYAGEEAVAVGVCAHLTDSDSITSTHRGHGHC
IAKGCDLNGMMAELFGKATGLCKGKGGSMHIADLDKGMLGANGIVGGGFPLACGSALTAKYKGTKDVSVCFFGDGANNEG
TFHEGVNLAAIWKLPVIFIAENNGYGEATTFEYASSCDSIADRAKAYNIPGVQVDGKDLLAVYKAAEEAVERARNGDGPT
IIECMTYRNYGHFEGEAQTYKTSEEKEEHLNEKDAIVNFRKHLIHEALLTESELVDMEKAVDDAVQKSIEFSENSPYPED
EELLKDVYVSYK

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=324, Percent_Identity=38.5802469135802, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI4505685, Length=320, Percent_Identity=37.1875, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI291084742, Length=320, Percent_Identity=37.1875, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI291084744, Length=327, Percent_Identity=36.3914373088685, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI291084757, Length=320, Percent_Identity=32.8125, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI11386135, Length=319, Percent_Identity=30.0940438871473, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI258645172, Length=319, Percent_Identity=30.0940438871473, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32564172, Length=318, Percent_Identity=38.3647798742138, Blast_Score=221, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17536047, Length=318, Percent_Identity=38.3647798742138, Blast_Score=221, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI86563355, Length=311, Percent_Identity=30.5466237942122, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI86563357, Length=311, Percent_Identity=30.5466237942122, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321026, Length=313, Percent_Identity=37.3801916932907, Blast_Score=213, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24639744, Length=318, Percent_Identity=39.3081761006289, Blast_Score=215, Evalue=3e-56,
Organism=Drosophila melanogaster, GI28571106, Length=318, Percent_Identity=39.3081761006289, Blast_Score=215, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24639740, Length=318, Percent_Identity=39.3081761006289, Blast_Score=215, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24639748, Length=322, Percent_Identity=39.4409937888199, Blast_Score=207, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24639746, Length=306, Percent_Identity=39.2156862745098, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI21355903, Length=326, Percent_Identity=26.9938650306748, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 36216; Mature: 36216

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTTESKGNGITKEQARWMYEKMLEIRKFEDKVHELFAQGVLPGFVHLYAGEEAVAVGV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEECCCCEEEEEE
CAHLTDSDSITSTHRGHGHCIAKGCDLNGMMAELFGKATGLCKGKGGSMHIADLDKGMLG
EEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEECCCCCEEEEECCCCCCC
ANGIVGGGFPLACGSALTAKYKGTKDVSVCFFGDGANNEGTFHEGVNLAAIWKLPVIFIA
CCCCCCCCCHHHCCCHHEECCCCCCCEEEEEEECCCCCCCCHHCCCCEEEEEECCEEEEE
ENNGYGEATTFEYASSCDSIADRAKAYNIPGVQVDGKDLLAVYKAAEEAVERARNGDGPT
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCE
IIECMTYRNYGHFEGEAQTYKTSEEKEEHLNEKDAIVNFRKHLIHEALLTESELVDMEKA
EEEEEEECCCCCCCCCCHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDDAVQKSIEFSENSPYPEDEELLKDVYVSYK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLKTTESKGNGITKEQARWMYEKMLEIRKFEDKVHELFAQGVLPGFVHLYAGEEAVAVGV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEECCCCEEEEEE
CAHLTDSDSITSTHRGHGHCIAKGCDLNGMMAELFGKATGLCKGKGGSMHIADLDKGMLG
EEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEECCCCCEEEEECCCCCCC
ANGIVGGGFPLACGSALTAKYKGTKDVSVCFFGDGANNEGTFHEGVNLAAIWKLPVIFIA
CCCCCCCCCHHHCCCHHEECCCCCCCEEEEEEECCCCCCCCHHCCCCEEEEEECCEEEEE
ENNGYGEATTFEYASSCDSIADRAKAYNIPGVQVDGKDLLAVYKAAEEAVERARNGDGPT
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCE
IIECMTYRNYGHFEGEAQTYKTSEEKEEHLNEKDAIVNFRKHLIHEALLTESELVDMEKA
EEEEEEECCCCCCCCCCHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDDAVQKSIEFSENSPYPEDEELLKDVYVSYK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]