Click here to switch to the map view.

The map label for this gene is gspS [H]

Identifier: 52142590

GI number: 52142590

Start: 2761937

End: 2763184

Strand: Reverse

Name: gspS [H]

Synonym: BCZK2652

Alternate gene names: 52142590

Gene position: 2763184-2761937 (Counterclockwise)

Preceding gene: 52142589

Following gene: 52142591

Centisome position: 52.13

GC content: 34.21

Gene sequence:

>1248_bases
ATGTATACAGAACAAGAACAGAAAGAGTATATGAAAGTATGGTTTTCACTTGCAGAAGAGGCTGGTCGGAATGGATTTAC
TTGGCCGAGTCTATTAGAAAATGAAGAATGGAATCAATATATGGCAACAGGTATGTATCGTATGCCAGTAAAAACATATA
CTGCTATTTCAAAAGCGACAGAAGAAATTATGTATGTGCTATATAGAACGTATCAATACATTCTCAATACGTCAAAAGAT
TTTCAAAAACTAGGATTTCCAGCTGAAACGTGGGAAATTGCGAGAATGAAACATACTGGTTTGTTTTCATATTTTACAAG
GTTTGACTTTATCGTAAATGGTGAAGATATAAAGTTAATAGAAGTAAATTGTGATACACCAACAGGTTATTTAGAACCGT
CTGTCGCAAATGAAGTGTTATGTCGTTATCACGATGTGAATCATCCAAATCATATAGAAGAGCATATTGTGCAGGCGTGG
GAACAAATTAAACATGATTATAGTATAGATCCTAGAGAAACGATTTATTTTACGAGTTATGATTGGCATGATGAAGACCA
TCAAACGGTTCAATTTTTAAGAAGCTATTGCTTAGATCAGTCAACGGATTATATAGGTATACAAGATATCGTCGTGGCAG
ATGATGGTATATATACGCCAAATGGTGAGAGAATTCATTATTTATATAGACTATATCCAATTGAATATTTAGTTTCAGAC
GCTGATAAAAGCGGGAAAAGAATTGGACTCCAGTTTTTAGATCATATAGCGCAAGGCAGAGTGAAAATCATTAATCCACC
AGCTGCTTTTCTTATGCAAAATAAAAGTGTACTCGCATTAATTTGGCAACTTTACGAAGAAGAAGTTTTCTTTAAGGAAG
AGGAGCGAGGAATCATTCAGAAATACTTCCTACCAACATATTTTACAAATAAACCGTTTATAGAAAGAAATAAATCCTAT
GTTTCTAAACCTTTATACGGTCGTGAAGGCGGAGGAGTATCTATATACGAAAATAATGAACTATTAGCTGAAGATAAAAC
AGAGTATTACTTTGAACAGCGGAAAATATATCAGCAATACATAGAAATGCCAGACTATACAATTGACACGTGGGACGGTC
CGTATACTGGTAAACTATTAATTGGTTCACACTGTATTAGCGGGAGAGCTGCTGGTTTATTTTTACGTGTAGGTGAGAAA
ATAACAGGAAATTTATCGATGTTTACTGGAGTTACAATTGAAGGATAA

Upstream 100 bases:

>100_bases
AATCATCTACGACACAAACGCCGAAAGTGAATTCTGGTTCTAGTGGAATTGGGAACGCGAAAGCGCCAGCAGGATCCTAA
AATGAAAGGATAATGGAGTA

Downstream 100 bases:

>100_bases
ATGAAACAAAGAGCTTATCATTTTTGGTGAGCTCTTTGTTTGCATATAGTGTTTTTATGTTTTTGATGTAACGCCTTCAT
TTTTTGCATAGATAAAAACG

Product: glutathionylspermidine synthetase

Products: N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine; ADP; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MYTEQEQKEYMKVWFSLAEEAGRNGFTWPSLLENEEWNQYMATGMYRMPVKTYTAISKATEEIMYVLYRTYQYILNTSKD
FQKLGFPAETWEIARMKHTGLFSYFTRFDFIVNGEDIKLIEVNCDTPTGYLEPSVANEVLCRYHDVNHPNHIEEHIVQAW
EQIKHDYSIDPRETIYFTSYDWHDEDHQTVQFLRSYCLDQSTDYIGIQDIVVADDGIYTPNGERIHYLYRLYPIEYLVSD
ADKSGKRIGLQFLDHIAQGRVKIINPPAAFLMQNKSVLALIWQLYEEEVFFKEEERGIIQKYFLPTYFTNKPFIERNKSY
VSKPLYGREGGGVSIYENNELLAEDKTEYYFEQRKIYQQYIEMPDYTIDTWDGPYTGKLLIGSHCISGRAAGLFLRVGEK
ITGNLSMFTGVTIEG

Sequences:

>Translated_415_residues
MYTEQEQKEYMKVWFSLAEEAGRNGFTWPSLLENEEWNQYMATGMYRMPVKTYTAISKATEEIMYVLYRTYQYILNTSKD
FQKLGFPAETWEIARMKHTGLFSYFTRFDFIVNGEDIKLIEVNCDTPTGYLEPSVANEVLCRYHDVNHPNHIEEHIVQAW
EQIKHDYSIDPRETIYFTSYDWHDEDHQTVQFLRSYCLDQSTDYIGIQDIVVADDGIYTPNGERIHYLYRLYPIEYLVSD
ADKSGKRIGLQFLDHIAQGRVKIINPPAAFLMQNKSVLALIWQLYEEEVFFKEEERGIIQKYFLPTYFTNKPFIERNKSY
VSKPLYGREGGGVSIYENNELLAEDKTEYYFEQRKIYQQYIEMPDYTIDTWDGPYTGKLLIGSHCISGRAAGLFLRVGEK
ITGNLSMFTGVTIEG
>Mature_415_residues
MYTEQEQKEYMKVWFSLAEEAGRNGFTWPSLLENEEWNQYMATGMYRMPVKTYTAISKATEEIMYVLYRTYQYILNTSKD
FQKLGFPAETWEIARMKHTGLFSYFTRFDFIVNGEDIKLIEVNCDTPTGYLEPSVANEVLCRYHDVNHPNHIEEHIVQAW
EQIKHDYSIDPRETIYFTSYDWHDEDHQTVQFLRSYCLDQSTDYIGIQDIVVADDGIYTPNGERIHYLYRLYPIEYLVSD
ADKSGKRIGLQFLDHIAQGRVKIINPPAAFLMQNKSVLALIWQLYEEEVFFKEEERGIIQKYFLPTYFTNKPFIERNKSY
VSKPLYGREGGGVSIYENNELLAEDKTEYYFEQRKIYQQYIEMPDYTIDTWDGPYTGKLLIGSHCISGRAAGLFLRVGEK
ITGNLSMFTGVTIEG

Specific function: Unknown

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To the N-terminal of E.coli gsp and to E.coli ygiC [H]

Homologues:

Organism=Escherichia coli, GI1790629, Length=419, Percent_Identity=26.0143198090692, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789416, Length=406, Percent_Identity=23.3990147783251, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005494
- InterPro:   IPR016185 [H]

Pfam domain/function: PF03738 GSP_synth [H]

EC number: 6.3.1.8

Molecular weight: Translated: 48789; Mature: 48789

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTEQEQKEYMKVWFSLAEEAGRNGFTWPSLLENEEWNQYMATGMYRMPVKTYTAISKAT
CCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCCEEECCHHHHHHHHHHH
EEIMYVLYRTYQYILNTSKDFQKLGFPAETWEIARMKHTGLFSYFTRFDFIVNGEDIKLI
HHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEE
EVNCDTPTGYLEPSVANEVLCRYHDVNHPNHIEEHIVQAWEQIKHDYSIDPRETIYFTSY
EEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
DWHDEDHQTVQFLRSYCLDQSTDYIGIQDIVVADDGIYTPNGERIHYLYRLYPIEYLVSD
CCCCCHHHHHHHHHHHHCCCCCCEECCEEEEEECCCEECCCCCEEEEEEEEHHHHHHHHC
ADKSGKRIGLQFLDHIAQGRVKIINPPAAFLMQNKSVLALIWQLYEEEVFFKEEERGIIQ
CCCCCHHHHHHHHHHHHCCCEEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHH
KYFLPTYFTNKPFIERNKSYVSKPLYGREGGGVSIYENNELLAEDKTEYYFEQRKIYQQY
HHHCCCCCCCCCHHHCCHHHHCCCCCCCCCCCEEEEECCCEECCCHHHHHHHHHHHHHHH
IEMPDYTIDTWDGPYTGKLLIGSHCISGRAAGLFLRVGEKITGNLSMFTGVTIEG
HCCCCCEEECCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MYTEQEQKEYMKVWFSLAEEAGRNGFTWPSLLENEEWNQYMATGMYRMPVKTYTAISKAT
CCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCCEEECCHHHHHHHHHHH
EEIMYVLYRTYQYILNTSKDFQKLGFPAETWEIARMKHTGLFSYFTRFDFIVNGEDIKLI
HHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEE
EVNCDTPTGYLEPSVANEVLCRYHDVNHPNHIEEHIVQAWEQIKHDYSIDPRETIYFTSY
EEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
DWHDEDHQTVQFLRSYCLDQSTDYIGIQDIVVADDGIYTPNGERIHYLYRLYPIEYLVSD
CCCCCHHHHHHHHHHHHCCCCCCEECCEEEEEECCCEECCCCCEEEEEEEEHHHHHHHHC
ADKSGKRIGLQFLDHIAQGRVKIINPPAAFLMQNKSVLALIWQLYEEEVFFKEEERGIIQ
CCCCCHHHHHHHHHHHHCCCEEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHH
KYFLPTYFTNKPFIERNKSYVSKPLYGREGGGVSIYENNELLAEDKTEYYFEQRKIYQQY
HHHCCCCCCCCCHHHCCHHHHCCCCCCCCCCCEEEEECCCEECCCHHHHHHHHHHHHHHH
IEMPDYTIDTWDGPYTGKLLIGSHCISGRAAGLFLRVGEKITGNLSMFTGVTIEG
HCCCCCEEECCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: gamma-L-glutamyl-L-cysteinyl-glycine; spermidine; ATP

Specific reaction: gamma-L-glutamyl-L-cysteinyl-glycine + spermidine + ATP = N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 7961409 [H]