| Definition | Bacillus cereus E33L, complete genome. |
|---|---|
| Accession | NC_006274 |
| Length | 5,300,915 |
Click here to switch to the map view.
The map label for this gene is ppsA [H]
Identifier: 52142418
GI number: 52142418
Start: 2939327
End: 2941936
Strand: Reverse
Name: ppsA [H]
Synonym: BCZK2825
Alternate gene names: 52142418
Gene position: 2941936-2939327 (Counterclockwise)
Preceding gene: 52142414
Following gene: 52142420
Centisome position: 55.5
GC content: 38.28
Gene sequence:
>2610_bases ATGAGTTCTTTCGTTCTCGATTTTCAGGAAATAGAAAAGGGGCAGCTTTCACTCGTCGGCGGAAAAGGTTTGAATTTAGG GGAGTTATCTAACATTCAAGGTATAGAGGTGCCAGAAGGATTTTGTGTGACAACGGTAGGATATGAAAAGGCCATCGAGC AAAATGAAAATCTTCAAACTTTGTTGCAGCAGCTGACCAAGTTGAAACTTGAAGACCGAGCTCAAATTGGTGAAATGAGT AAGGAAATTAGAGAAGTCATTATGGCAGTACAAATCCCTTCTGATGTAGTGGAAGCGGTAGCTCATTATCTCTCACGTTT TGGAAATGAACATGCGTATGCCGTGCGTTCTAGTGCTACGGCTGAAGATTTACCGTATGCCTCGTTTGCAGGTCAACAAG ATACGTATTTAAATATTATCGGAAAAGAAGCGATTTTACAGCATGTAAGAAAGTGCTGGGCTTCTTTATTTACAGAGCGA GCAGTCATGTACCGTATGCAAAATGGTTTTAAACATAATCAAGTTTCTATATGTGTTGTCGTTCAAAAAATGGTTTTCCC TGAGGCTTCAGGCATTTTATTTACTGCCGATCCGATTACTTCTAACCGGAAGGTGTTATCAATTGATGCCAGTTTTGGAC TTGGCGAGGCATTAGTATCGGGACTCGTCTCTGCCGATAATTATAAAGTAATAGAGGGGAAAATCGTCGATAAGGTGATT TCAACTAAAAAAGTAGCTATCTATGCCTTAAAAGAGGGCGGAACAGAGACGAAACAGATTAATTCGGCTCAGCAAAAGAT TCAAACATTGTCTGAACAGCAAATATTACAACTCGCACAGATTGGAAGACAGATAGAAGCTTATTTTGGCTATCCGCAAG ATATTGAATGGTGTTTAGTTGATAATGCATTTTATATTGTCCAAAGTCGTCCGATAACGACTTTATATCCAATTCCGGAA GAAAATGATGAGGGAAATCATGTGTATATATCAGTTGGTCATCAACAAATGATGACCGATGCGATGAAACCATTAGGGTT ATCTTTTTTCTTGTTAACGACTAATGCTCCTATGCGTAAAGCGGGAGGAAGGCTGTTTGTGGATGCTACACAAAGATTAG CATCACCTGCTAGCAGAGATTACCTAATAAATACGTTAGGAAAATCAGATCCGCTCATAAGAGATGCATTAACGACTATA ATCGAGCGAGATGATTTTATTACATTATTACCGGATGAAGAAAAAAAAGAAAAGAGTGCTAGTAAAAGTAAGCTACCAGT AAGTTCGCAACCAGAAATCGAAAATGATCCAGCAATCGTTACGGAATTAATAAGGAATAGTGAAGCATCACTTGAAGAGT TAAAACAAAATATGCAACTGAAATCAGGAGTGGATGTATTTGATTTTATTTTGGAGGACATCCAGCAATTAAAGAAAGTG TTATTTAATCCGCAAAGTATAGCTGTCATCATGGCAGGTATGAATGCTTCAACATGGATGAATGAAAAAATGGAGCAGTG GCTAGGTGAAAAGAATGCAGCAGACACGCTTTCTCAATCGGTGCAAAATAATATTACGTCGGAAATGGGTTTAGCATTAA TGGATGTTGCTGATGCAATTCGTCCGTATCCAGAAGTTATTACGTATTTACAACATGTAGAAAATGATAGCTTTTTAGAT GAATTCGTTCAGTTTAAAGGCGGAGAAAAAGCTCGAGAGGCAATCGATGCTTTTCTAAATAAATATGGAATGAGATGTAG CGGAGAAATCGACATTACGAAAACGCGCTGGAGTGAGAAGCCAACAACCATTATCCCGATGATTTTAAATAATATAAGAG ATTTTGAATATGGTGCAAGTAAACGGAAATTTGAAGAAGGACTGCAAGAAGCTTTGAAAAAAGAAGAAGAGTTAGTAGAT CGATTGCAGCAATTGCCGGATGGAAAACAAAAAGTAGAAGAAACGAAGCGAATGATTCGCAATATCCGTAATTTTATCGG CTATCGTGAATATCCGAAATACGGTATGATTAATCGTTATTTCATATATAAGCAGGCGTTACTGAAAGAAGCTGAGCAAC TTGTGCAGAGCGGTGTCATTCATGAAGTTGATGATATATACTATTTAACTTTTGAAGAGCTTCACGAAGTTGTTCGCACG AAGAAATTAGATTACGAAATTATTCATAAACAAAAAAATGATTATAAATTATACGAAAAACTAACGCCTCCTCGTATAAT GACGTCTGATGGAGAAATCATTACAGGGAAATATAAACGAGAAAACCTCCCAGCTGACGCGATTGCAGGTCTGCCTGTTT CTTCTGGAGTAGTTGAGGGGAGAGCTCGCGTTATTTTAAACATGGAAGATGCGAATTTAGAAGAGGGAGATATATTAGTT ACGGCTTTTACTGACCCTGGCTGGACACCATTATTTGTATCTATAAAAGGATTAGTTACTGAAGTCGGCGGACTTATGAC GCATGGAGCAGTTATCGCACGTGAATATGGATTACCAGCAGTCGTTGGGGTAGAGAATGCTACGAAATTAATAAAAGATG GCCAACGAATTCGGGTACATGGAACAGAAGGGTATATTGAAGTGTTGTAA
Upstream 100 bases:
>100_bases AACTTAATTATATCATGTAAATCAAATTCGAAATAGACTGTTTCTTCTCATTTACTCCTGCTACAATCAAGAACACATGC ATAGTAAGGAGAGATGTTAT
Downstream 100 bases:
>100_bases TGAAGTAAAAGATCATCCGCTATAAATAGTGGATGATCTTTTTATTTTGCAACAACTCACTTTTTTAACCCAGCTGCATA AAAAGGTCCATGAACTGCGA
Product: phosphoenolpyruvate synthase
Products: AMP; phosphoenolpyruvate; phosphate
Alternate protein names: Putative PEP synthase; Pyruvate, water dikinase [H]
Number of amino acids: Translated: 869; Mature: 868
Protein sequence:
>869_residues MSSFVLDFQEIEKGQLSLVGGKGLNLGELSNIQGIEVPEGFCVTTVGYEKAIEQNENLQTLLQQLTKLKLEDRAQIGEMS KEIREVIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGKEAILQHVRKCWASLFTER AVMYRMQNGFKHNQVSICVVVQKMVFPEASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSADNYKVIEGKIVDKVI STKKVAIYALKEGGTETKQINSAQQKIQTLSEQQILQLAQIGRQIEAYFGYPQDIEWCLVDNAFYIVQSRPITTLYPIPE ENDEGNHVYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRKAGGRLFVDATQRLASPASRDYLINTLGKSDPLIRDALTTI IERDDFITLLPDEEKKEKSASKSKLPVSSQPEIENDPAIVTELIRNSEASLEELKQNMQLKSGVDVFDFILEDIQQLKKV LFNPQSIAVIMAGMNASTWMNEKMEQWLGEKNAADTLSQSVQNNITSEMGLALMDVADAIRPYPEVITYLQHVENDSFLD EFVQFKGGEKAREAIDAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEYGASKRKFEEGLQEALKKEEELVD RLQQLPDGKQKVEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVIHEVDDIYYLTFEELHEVVRT KKLDYEIIHKQKNDYKLYEKLTPPRIMTSDGEIITGKYKRENLPADAIAGLPVSSGVVEGRARVILNMEDANLEEGDILV TAFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKLIKDGQRIRVHGTEGYIEVL
Sequences:
>Translated_869_residues MSSFVLDFQEIEKGQLSLVGGKGLNLGELSNIQGIEVPEGFCVTTVGYEKAIEQNENLQTLLQQLTKLKLEDRAQIGEMS KEIREVIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGKEAILQHVRKCWASLFTER AVMYRMQNGFKHNQVSICVVVQKMVFPEASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSADNYKVIEGKIVDKVI STKKVAIYALKEGGTETKQINSAQQKIQTLSEQQILQLAQIGRQIEAYFGYPQDIEWCLVDNAFYIVQSRPITTLYPIPE ENDEGNHVYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRKAGGRLFVDATQRLASPASRDYLINTLGKSDPLIRDALTTI IERDDFITLLPDEEKKEKSASKSKLPVSSQPEIENDPAIVTELIRNSEASLEELKQNMQLKSGVDVFDFILEDIQQLKKV LFNPQSIAVIMAGMNASTWMNEKMEQWLGEKNAADTLSQSVQNNITSEMGLALMDVADAIRPYPEVITYLQHVENDSFLD EFVQFKGGEKAREAIDAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEYGASKRKFEEGLQEALKKEEELVD RLQQLPDGKQKVEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVIHEVDDIYYLTFEELHEVVRT KKLDYEIIHKQKNDYKLYEKLTPPRIMTSDGEIITGKYKRENLPADAIAGLPVSSGVVEGRARVILNMEDANLEEGDILV TAFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKLIKDGQRIRVHGTEGYIEVL >Mature_868_residues SSFVLDFQEIEKGQLSLVGGKGLNLGELSNIQGIEVPEGFCVTTVGYEKAIEQNENLQTLLQQLTKLKLEDRAQIGEMSK EIREVIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGKEAILQHVRKCWASLFTERA VMYRMQNGFKHNQVSICVVVQKMVFPEASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSADNYKVIEGKIVDKVIS TKKVAIYALKEGGTETKQINSAQQKIQTLSEQQILQLAQIGRQIEAYFGYPQDIEWCLVDNAFYIVQSRPITTLYPIPEE NDEGNHVYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRKAGGRLFVDATQRLASPASRDYLINTLGKSDPLIRDALTTII ERDDFITLLPDEEKKEKSASKSKLPVSSQPEIENDPAIVTELIRNSEASLEELKQNMQLKSGVDVFDFILEDIQQLKKVL FNPQSIAVIMAGMNASTWMNEKMEQWLGEKNAADTLSQSVQNNITSEMGLALMDVADAIRPYPEVITYLQHVENDSFLDE FVQFKGGEKAREAIDAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEYGASKRKFEEGLQEALKKEEELVDR LQQLPDGKQKVEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVIHEVDDIYYLTFEELHEVVRTK KLDYEIIHKQKNDYKLYEKLTPPRIMTSDGEIITGKYKRENLPADAIAGLPVSSGVVEGRARVILNMEDANLEEGDILVT AFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKLIKDGQRIRVHGTEGYIEVL
Specific function: Might catalyze the phosphorylation of pyruvate to phosphoenolpyruvate (Potential) [H]
COG id: COG0574
COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the PEP-utilizing enzyme family [H]
Homologues:
Organism=Escherichia coli, GI1787994, Length=334, Percent_Identity=34.4311377245509, Blast_Score=177, Evalue=2e-45, Organism=Caenorhabditis elegans, GI17564524, Length=301, Percent_Identity=32.2259136212625, Blast_Score=127, Evalue=3e-29,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013815 - InterPro: IPR013816 - InterPro: IPR008279 - InterPro: IPR002192 [H]
Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]
EC number: 2.7.9.2
Molecular weight: Translated: 97660; Mature: 97529
Theoretical pI: Translated: 4.89; Mature: 4.89
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.6 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 3.3 %Cys+Met (Translated Protein) 0.6 %Cys (Mature Protein) 2.6 %Met (Mature Protein) 3.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSSFVLDFQEIEKGQLSLVGGKGLNLGELSNIQGIEVPEGFCVTTVGYEKAIEQNENLQT CCCCEECHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHCCCCHHH LLQQLTKLKLEDRAQIGEMSKEIREVIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSAT HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCC AEDLPYASFAGQQDTYLNIIGKEAILQHVRKCWASLFTERAVMYRMQNGFKHNQVSICVV CCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHH VQKMVFPEASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSADNYKVIEGKIVDKVI HHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEHHHHHHHHH STKKVAIYALKEGGTETKQINSAQQKIQTLSEQQILQLAQIGRQIEAYFGYPQDIEWCLV CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE DNAFYIVQSRPITTLYPIPEENDEGNHVYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRK CCEEEEEECCCCEEEECCCCCCCCCCEEEEEECHHHHHHHHHHHCCCEEEEEECCCCHHH AGGRLFVDATQRLASPASRDYLINTLGKSDPLIRDALTTIIERDDFITLLPDEEKKEKSA CCCEEEEEHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHH SKSKLPVSSQPEIENDPAIVTELIRNSEASLEELKQNMQLKSGVDVFDFILEDIQQLKKV HHCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH LFNPQSIAVIMAGMNASTWMNEKMEQWLGEKNAADTLSQSVQNNITSEMGLALMDVADAI HCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH RPYPEVITYLQHVENDSFLDEFVQFKGGEKAREAIDAFLNKYGMRCSGEIDITKTRWSEK CCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC PTTIIPMILNNIRDFEYGASKRKFEEGLQEALKKEEELVDRLQQLPDGKQKVEETKRMIR CCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH NIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVIHEVDDIYYLTFEELHEVVRT HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHH KKLDYEIIHKQKNDYKLYEKLTPPRIMTSDGEIITGKYKRENLPADAIAGLPVSSGVVEG HCCCHHHHHCCCCCHHHHHHCCCCCEEECCCCEEECCCCCCCCCHHHHCCCCCCCCCCCC RARVILNMEDANLEEGDILVTAFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPA CEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCE VVGVENATKLIKDGQRIRVHGTEGYIEVL EEECHHHHHHHHCCCEEEEECCCCEEEEC >Mature Secondary Structure SSFVLDFQEIEKGQLSLVGGKGLNLGELSNIQGIEVPEGFCVTTVGYEKAIEQNENLQT CCCEECHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHCCCCHHH LLQQLTKLKLEDRAQIGEMSKEIREVIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSAT HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCC AEDLPYASFAGQQDTYLNIIGKEAILQHVRKCWASLFTERAVMYRMQNGFKHNQVSICVV CCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHH VQKMVFPEASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSADNYKVIEGKIVDKVI HHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEHHHHHHHHH STKKVAIYALKEGGTETKQINSAQQKIQTLSEQQILQLAQIGRQIEAYFGYPQDIEWCLV CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE DNAFYIVQSRPITTLYPIPEENDEGNHVYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRK CCEEEEEECCCCEEEECCCCCCCCCCEEEEEECHHHHHHHHHHHCCCEEEEEECCCCHHH AGGRLFVDATQRLASPASRDYLINTLGKSDPLIRDALTTIIERDDFITLLPDEEKKEKSA CCCEEEEEHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHH SKSKLPVSSQPEIENDPAIVTELIRNSEASLEELKQNMQLKSGVDVFDFILEDIQQLKKV HHCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH LFNPQSIAVIMAGMNASTWMNEKMEQWLGEKNAADTLSQSVQNNITSEMGLALMDVADAI HCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH RPYPEVITYLQHVENDSFLDEFVQFKGGEKAREAIDAFLNKYGMRCSGEIDITKTRWSEK CCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC PTTIIPMILNNIRDFEYGASKRKFEEGLQEALKKEEELVDRLQQLPDGKQKVEETKRMIR CCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH NIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVIHEVDDIYYLTFEELHEVVRT HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHH KKLDYEIIHKQKNDYKLYEKLTPPRIMTSDGEIITGKYKRENLPADAIAGLPVSSGVVEG HCCCHHHHHCCCCCHHHHHHCCCCCEEECCCCEEECCCCCCCCCHHHHCCCCCCCCCCCC RARVILNMEDANLEEGDILVTAFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPA CEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCE VVGVENATKLIKDGQRIRVHGTEGYIEVL EEECHHHHHHHHCCCEEEEECCCCEEEEC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: Mg2+; Mn2+ [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.083 {pyruvate}} 10.5 {phosphate}} 0.028 {ATP}} [C]
Substrates: ATP; pyruvate; H2O
Specific reaction: ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate
General reaction: Phospho group transfer [C]
Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9384377; 7704256 [H]