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The map label for this gene is adaA [H]

Identifier: 52142328

GI number: 52142328

Start: 3042187

End: 3042783

Strand: Reverse

Name: adaA [H]

Synonym: BCZK2914

Alternate gene names: 52142328

Gene position: 3042783-3042187 (Counterclockwise)

Preceding gene: 52142329

Following gene: 52142327

Centisome position: 57.4

GC content: 35.68

Gene sequence:

>597_bases
ATGAAGGATCAAAATAACCTTTCCAGAGATACCATGTGGAAAGCAACAGTAACATGTGATTCAGCGTATGACGGGATATT
CTTTTACGCAGTCAAGACAACTAGAATTTTCTGTCGGCCTTCTTGCAAGTCTAAGGTTCCTAATTATGAAAATGTATCCT
TTTTTTCAAATGCGCAAGAAGCTCAAAGGGCAGGCTATCGCCCATGCAAAAGATGCCGATCAGATCTAAATATAAAAATA
TACGATCCGTTCGAATGCGTTTTGGAAGATACTATGCGATTGATAAAAAATAATTATGTTCAAAACATGTCTTTAAATGA
AATTGCATCAAGGGTAGGAGTTAGTCGTTTTCATTTAAATCGGATTTTCAAAGAAAGAACAGGGTATACTCCTCGAATAT
ATTTAGAAAGGATCAGAGTTAAGAAAGCAAAGGAACTTCTCTTGACAACGGTCTTTAATAGTACAGAGATTGGTTATCAA
ACAGGCTATCAAAGTGTATCTAGCTTTTATAATGCATTTAAAAGAAATACAGGATTTTCACCGAGTCAATTCCGTGCTTT
CCATAGTGGAAATACCGTAAACCAAGAACTTGACTGA

Upstream 100 bases:

>100_bases
CAATTGTATATATTTGTTCCGTTAATTGTCGTGGCTATATATACGATTTATATGATTTTGAGTAATAAGATTTTCGCCTA
CAAGGGAGGATTAAAATCTA

Downstream 100 bases:

>100_bases
ATTCACGTTCTTTAACAAAAGATAGCATGAATTAACAAGAGTGCTTTGTACTCATCGTATTTTTCGATAAATCATTACTG
ATTGATTAAAAGTAAACAAT

Product: methylphosphotriester-DNA alkyltransferase

Products: DNA; protein S-methyl-L-cysteine

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MKDQNNLSRDTMWKATVTCDSAYDGIFFYAVKTTRIFCRPSCKSKVPNYENVSFFSNAQEAQRAGYRPCKRCRSDLNIKI
YDPFECVLEDTMRLIKNNYVQNMSLNEIASRVGVSRFHLNRIFKERTGYTPRIYLERIRVKKAKELLLTTVFNSTEIGYQ
TGYQSVSSFYNAFKRNTGFSPSQFRAFHSGNTVNQELD

Sequences:

>Translated_198_residues
MKDQNNLSRDTMWKATVTCDSAYDGIFFYAVKTTRIFCRPSCKSKVPNYENVSFFSNAQEAQRAGYRPCKRCRSDLNIKI
YDPFECVLEDTMRLIKNNYVQNMSLNEIASRVGVSRFHLNRIFKERTGYTPRIYLERIRVKKAKELLLTTVFNSTEIGYQ
TGYQSVSSFYNAFKRNTGFSPSQFRAFHSGNTVNQELD
>Mature_198_residues
MKDQNNLSRDTMWKATVTCDSAYDGIFFYAVKTTRIFCRPSCKSKVPNYENVSFFSNAQEAQRAGYRPCKRCRSDLNIKI
YDPFECVLEDTMRLIKNNYVQNMSLNEIASRVGVSRFHLNRIFKERTGYTPRIYLERIRVKKAKELLLTTVFNSTEIGYQ
TGYQSVSSFYNAFKRNTGFSPSQFRAFHSGNTVNQELD

Specific function: Methylphosphotriester-DNA alkyltransferase is one of two genes required for the adaptive response of bacteria to alkylating agents. It accepts a methyl group from methylphospho-triesters and then acts as a transcriptional activator of the ada operon [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=193, Percent_Identity=35.2331606217617, Blast_Score=121, Evalue=3e-29,
Organism=Escherichia coli, GI1789993, Length=159, Percent_Identity=27.0440251572327, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR016220 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF00165 HTH_AraC [H]

EC number: 2.1.1.63

Molecular weight: Translated: 23073; Mature: 23073

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDQNNLSRDTMWKATVTCDSAYDGIFFYAVKTTRIFCRPSCKSKVPNYENVSFFSNAQE
CCCCCCCCHHHEEEEEEEECCCCCCEEEEEEEEEEEEECCCHHHCCCCCCCCHHHHCHHH
AQRAGYRPCKRCRSDLNIKIYDPFECVLEDTMRLIKNNYVQNMSLNEIASRVGVSRFHLN
HHHCCCHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RIFKERTGYTPRIYLERIRVKKAKELLLTTVFNSTEIGYQTGYQSVSSFYNAFKRNTGFS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PSQFRAFHSGNTVNQELD
HHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKDQNNLSRDTMWKATVTCDSAYDGIFFYAVKTTRIFCRPSCKSKVPNYENVSFFSNAQE
CCCCCCCCHHHEEEEEEEECCCCCCEEEEEEEEEEEEECCCHHHCCCCCCCCHHHHCHHH
AQRAGYRPCKRCRSDLNIKIYDPFECVLEDTMRLIKNNYVQNMSLNEIASRVGVSRFHLN
HHHCCCHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RIFKERTGYTPRIYLERIRVKKAKELLLTTVFNSTEIGYQTGYQSVSSFYNAFKRNTGFS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PSQFRAFHSGNTVNQELD
HHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA containing 6-O-methylguanine; protein L-cysteine

Specific reaction: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]