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The map label for this gene is yhcA [H]

Identifier: 52142320

GI number: 52142320

Start: 3053520

End: 3054998

Strand: Reverse

Name: yhcA [H]

Synonym: BCZK2922

Alternate gene names: 52142320

Gene position: 3054998-3053520 (Counterclockwise)

Preceding gene: 52142318

Following gene: 52142321

Centisome position: 57.63

GC content: 38.61

Gene sequence:

>1479_bases
ATGAGTGAAACGTTACATAAAAGAAAACCACCATATGTAATGCTTGTAATCCTTTTTATTGGGGCATTTGTTTCATTTCT
AAATAACTCGCTTTTAAATGTGGCCTTGCCATCAATAATGAAGGATTTAGATATTAAAGATTATTCAACGATCCAGTGGC
TTTCTACAGGGTATATGCTTGTAAGTGGTATATTAATTCCGGCATCAGCATTTTTAATAACGCGCTTCTCCAATAGGAGC
TTATTTATTACATCTATGGTGATTTTTATTCTTGGTACGGCCTTAGCGGCTGTAGCACCGAACTTCGGTTTATTACTTAC
TGGTCGGATGGTTCAAGCAGCAGGTTCTTCAGTGATGGGACCTTTGTTAATGAATATTATGCTAGTAAGCTTTCCGAGAG
AAAAAAGGGGAACAGCAATGGGGATTTTTGGGTTAGTTATGATTACAGCTCCAGCAATTGGACCGACACTATCAGGTTAT
ATTGTAGAGTATTATGACTGGCGTTTGCTTTTTGAAATGATTTTACCGCTAGCAATCATTAGCTTACTGTTAGGGATTTG
GAAATCGGAAAATGTCATGAGACAAAACAAAAATGCAAAACTTGATTATCTTTCATTACTATTATCTTCTATCGGTTTTG
GCGGTTTATTATATGGGTTCAGTTCTGCAAGCTCCGATGGTTGGACGAATAAAGTTGTCGTAACAACCTTAACCATAGGC
GCTATTGCCCTAATCGCTTTCATTATCCGTCAATTAAAAATGAATGAACCGTTATTGGATTTACGTGTTTATAAGTACCC
AATGTTTGCGCTTGCGTCTGTAATTGCAATTGTCAATGCGGTAGCTATGTTTTCGGGTATGATTTTAACACCAGCTTATG
TACAAAACGTTCGCGGTATTTCACCGCTGAGCTCTGGGCTGATGATGCTTCCAGGGGCGGTAATAATGGGGATTATGTCA
CCAATTACAGGTAAACTATTTGATAAATATGGCCCTCGTATTCTTGGTATAGTAGGACTTTCCATTACAGCCGTTTCAAC
TTATATGCTAGCAAACCTACAACTCGACTCTAGTCACACACATATTATTCTTATTTACACACTGCGGATGTTTGGGATGG
CGATGGTGATGATGCCACTTATGACAAATGGCCTTAATCAATTGCCAACGCGCTTAAATCCGCACGGTACAGCTGTTAAT
AATACAGCTCAACAAGTATCCGGTTCAATTGGTACTGCTATTCTTGTTACCATTATGAATTCTGTAACAAAAACAAAAGC
CGAAAGTTTAATGGCTCATGTAGACCCAACAAACTTCACAGAAGCTACGAAGATAATGTTAACGCAGAAGGCTTTGTTAT
CCGGAATTCAGCATTCATTCTATGTAGCACTTGGCATGAATGTCGTTGCGCTACTATTAGTTTTTTTTGTTAAACGTGTA
GATACAAGCCCGGAAGCCGTTGAGAGGTTAAATCGATAG

Upstream 100 bases:

>100_bases
AGAGTTTGGCAAAATACTGCAAGAGTAATAAATTCTCGTGCAAATATGCAAACGAGTTAAATTAATCAATGGAATTGATA
ATGAAGGGGCAAATCAATCT

Downstream 100 bases:

>100_bases
AGCAAAATAAAAATCAAGATGATGATTTCTACTAAAAGGGAAAAGAGCTGAAAGAAAAAGTGAAATAATTACACAGAATG
AAAAGGATCTGATATTCATA

Product: multidrug resistance protein B

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLDIKDYSTIQWLSTGYMLVSGILIPASAFLITRFSNRS
LFITSMVIFILGTALAAVAPNFGLLLTGRMVQAAGSSVMGPLLMNIMLVSFPREKRGTAMGIFGLVMITAPAIGPTLSGY
IVEYYDWRLLFEMILPLAIISLLLGIWKSENVMRQNKNAKLDYLSLLLSSIGFGGLLYGFSSASSDGWTNKVVVTTLTIG
AIALIAFIIRQLKMNEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRGISPLSSGLMMLPGAVIMGIMS
PITGKLFDKYGPRILGIVGLSITAVSTYMLANLQLDSSHTHIILIYTLRMFGMAMVMMPLMTNGLNQLPTRLNPHGTAVN
NTAQQVSGSIGTAILVTIMNSVTKTKAESLMAHVDPTNFTEATKIMLTQKALLSGIQHSFYVALGMNVVALLLVFFVKRV
DTSPEAVERLNR

Sequences:

>Translated_492_residues
MSETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLDIKDYSTIQWLSTGYMLVSGILIPASAFLITRFSNRS
LFITSMVIFILGTALAAVAPNFGLLLTGRMVQAAGSSVMGPLLMNIMLVSFPREKRGTAMGIFGLVMITAPAIGPTLSGY
IVEYYDWRLLFEMILPLAIISLLLGIWKSENVMRQNKNAKLDYLSLLLSSIGFGGLLYGFSSASSDGWTNKVVVTTLTIG
AIALIAFIIRQLKMNEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRGISPLSSGLMMLPGAVIMGIMS
PITGKLFDKYGPRILGIVGLSITAVSTYMLANLQLDSSHTHIILIYTLRMFGMAMVMMPLMTNGLNQLPTRLNPHGTAVN
NTAQQVSGSIGTAILVTIMNSVTKTKAESLMAHVDPTNFTEATKIMLTQKALLSGIQHSFYVALGMNVVALLLVFFVKRV
DTSPEAVERLNR
>Mature_491_residues
SETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLDIKDYSTIQWLSTGYMLVSGILIPASAFLITRFSNRSL
FITSMVIFILGTALAAVAPNFGLLLTGRMVQAAGSSVMGPLLMNIMLVSFPREKRGTAMGIFGLVMITAPAIGPTLSGYI
VEYYDWRLLFEMILPLAIISLLLGIWKSENVMRQNKNAKLDYLSLLLSSIGFGGLLYGFSSASSDGWTNKVVVTTLTIGA
IALIAFIIRQLKMNEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRGISPLSSGLMMLPGAVIMGIMSP
ITGKLFDKYGPRILGIVGLSITAVSTYMLANLQLDSSHTHIILIYTLRMFGMAMVMMPLMTNGLNQLPTRLNPHGTAVNN
TAQQVSGSIGTAILVTIMNSVTKTKAESLMAHVDPTNFTEATKIMLTQKALLSGIQHSFYVALGMNVVALLLVFFVKRVD
TSPEAVERLNR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=451, Percent_Identity=26.8292682926829, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789042, Length=487, Percent_Identity=24.8459958932238, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI87081983, Length=411, Percent_Identity=26.0340632603406, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1790195, Length=402, Percent_Identity=26.1194029850746, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788392, Length=405, Percent_Identity=25.9259259259259, Blast_Score=93, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322958, Length=522, Percent_Identity=21.455938697318, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323735, Length=328, Percent_Identity=23.1707317073171, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53560; Mature: 53429

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLDIKDYSTIQWLSTGYML
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VSGILIPASAFLITRFSNRSLFITSMVIFILGTALAAVAPNFGLLLTGRMVQAAGSSVMG
HHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHCCCHHHH
PLLMNIMLVSFPREKRGTAMGIFGLVMITAPAIGPTLSGYIVEYYDWRLLFEMILPLAII
HHHHHHHHHHCCCHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLLLGIWKSENVMRQNKNAKLDYLSLLLSSIGFGGLLYGFSSASSDGWTNKVVVTTLTIG
HHHHHHHCCCHHHHCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEEEHHHHH
AIALIAFIIRQLKMNEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRGI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHCCCC
SPLSSGLMMLPGAVIMGIMSPITGKLFDKYGPRILGIVGLSITAVSTYMLANLQLDSSHT
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHEEEECCCCC
HIILIYTLRMFGMAMVMMPLMTNGLNQLPTRLNPHGTAVNNTAQQVSGSIGTAILVTIMN
EEEHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHH
SVTKTKAESLMAHVDPTNFTEATKIMLTQKALLSGIQHSFYVALGMNVVALLLVFFVKRV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DTSPEAVERLNR
CCCHHHHHHHCC
>Mature Secondary Structure 
SETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLDIKDYSTIQWLSTGYML
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VSGILIPASAFLITRFSNRSLFITSMVIFILGTALAAVAPNFGLLLTGRMVQAAGSSVMG
HHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHCCCHHHH
PLLMNIMLVSFPREKRGTAMGIFGLVMITAPAIGPTLSGYIVEYYDWRLLFEMILPLAII
HHHHHHHHHHCCCHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLLLGIWKSENVMRQNKNAKLDYLSLLLSSIGFGGLLYGFSSASSDGWTNKVVVTTLTIG
HHHHHHHCCCHHHHCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEEEHHHHH
AIALIAFIIRQLKMNEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRGI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHCCCC
SPLSSGLMMLPGAVIMGIMSPITGKLFDKYGPRILGIVGLSITAVSTYMLANLQLDSSHT
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHEEEECCCCC
HIILIYTLRMFGMAMVMMPLMTNGLNQLPTRLNPHGTAVNNTAQQVSGSIGTAILVTIMN
EEEHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHH
SVTKTKAESLMAHVDPTNFTEATKIMLTQKALLSGIQHSFYVALGMNVVALLLVFFVKRV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DTSPEAVERLNR
CCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]