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The map label for this gene is 52142243

Identifier: 52142243

GI number: 52142243

Start: 3129225

End: 3130763

Strand: Reverse

Name: 52142243

Synonym: BCZK2999

Alternate gene names: NA

Gene position: 3130763-3129225 (Counterclockwise)

Preceding gene: 52142240

Following gene: 52142247

Centisome position: 59.06

GC content: 33.85

Gene sequence:

>1539_bases
GTGATTTTTCTGAACATTAAGAAAAATTCCAAGAGAAGAAAGTTCCTTACATGTGTATTAGTTAGTCTATGCACTCTTAA
TTATTCATCTATTTCTTTCGCAGAAACACAAGCCGGTCATGCAACTGATATAACTAAAAATGCTAGTAGCATTGATACTG
GTATAGGAAATCTTACATATAATAATCAAGAGGTTTTAGCTGTAAATGGTGATAAAGTAGAGAGTTTTGTTCCGAAAGAA
AGTATCAATTCAAATGGTAAATTTGTAGTAGTGGAACGTGAGAAAAAATCACTTACAACGTCACCAGTTGATATTTCGAT
TATTGATTCTGTAGCGAATCGTACGTATCCAGGAGCTGTACAACTTGCCAATAAAGCTTTTGCAGACAATCAACCGAGTT
TATTAGTGGCTAAGAGAAAGCCTTTGAATATTAGTATAGACTTACCTGGTATGAGAAAAGAAAATACAATCACTGTCCAA
AATCCGACATATGGTAATGTGGCTGGAGCAGTAGATGATTTAGTATCTACTTGGAATGAAAAGTATTCCACAACACATAC
GTTACCTGCAAGAATGCAGTATACAGAATCTATGGTTTATAGTAAATCACAAATCGCAAGTGCTCTTAATGTTAATGCTA
AATATCTTGATAACAGTCTAAATATTGACTTTAATGCAGTTGCAAATGGAGAAAAGAAAGTGATGGTGGCGGCATATAAA
CAAATATTTTATACTGTAAGTGCTGAACTACCTAATAATCCATCTGATCTCTTTGATAATAGTGTTACTTTTGATGAGCT
AACTCGTAAAGGAGTAAGTAATTCGGCTCCACCTGTTATGGTTTCAAATGTAGCTTATGGTAGAACGGTTTATGTAAAAT
TAGAAACAACATCTAAGAGTAAAGATGTACAAGCTGCTTTTAAAGCTTTACTTAAGAATAACAGCGTCGAAACGAGTGGA
CAGTACAAAGATATTTTTGAAGAAAGTACCTTTACGGCTGTCGTATTAGGCGGAGATGCGAAAGAGCATAACAAGGTTGT
TACAAAAGATTTCAATGAAATCCGAAATATTATTAAAGATAATGCTGAATTAAGTTTTAAAAATCCAGCATACCCAATTT
CATATACAAGCACGTTCTTAAAAGATAATGCAACTGCTGCTGTTCATAACAATACAGATTATATCGAGACGACAACTACA
GAATATTCAAGTGCTAAAATGACACTCGATCATTACGGTGCTTACGTTGCTCAATTTGATGTATCTTGGGATGAATTCAC
ATTTGATCAAAATGGGAAAGAAGTACTAACACATAAAACATGGGAAGGTAGTGGCAAAGACAAAACAGCCCATTACTCTA
CAGTTATTCCTCTTCCACCAAATTCAAAAAATATAAAAATCGTTGCAAGAGAATGTACAGGTCTTGCATGGGAATGGTGG
AGAACAATTATTAATGAACAAAATGTTCCATTAACAAATGAAATAAAGGTTTCAATTGGAGGAACTACATTATACCCAAC
TGCTACTATTAGTCATTAG

Upstream 100 bases:

>100_bases
TAATTCCTTTAGTTCAAGAACTTTTAAAAGCGTTTATGTAAGTTTATTCGTTAGCTATCATGTATCCATCATTATTATAT
CTTACTAAAAAAAGAATGGG

Downstream 100 bases:

>100_bases
GTTTGAGTGAAGCGCCTTGTTTATAGGGCGCTTTTTTTACTTAAGTGAAAGTAAATTGGCGTTTCTTAAATATATCATAA
TCAATTCGCAAATCTTGTCA

Product: perfringolysin O, thiol-activated cytolysin

Products: NA

Alternate protein names: Fragment [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MIFLNIKKNSKRRKFLTCVLVSLCTLNYSSISFAETQAGHATDITKNASSIDTGIGNLTYNNQEVLAVNGDKVESFVPKE
SINSNGKFVVVEREKKSLTTSPVDISIIDSVANRTYPGAVQLANKAFADNQPSLLVAKRKPLNISIDLPGMRKENTITVQ
NPTYGNVAGAVDDLVSTWNEKYSTTHTLPARMQYTESMVYSKSQIASALNVNAKYLDNSLNIDFNAVANGEKKVMVAAYK
QIFYTVSAELPNNPSDLFDNSVTFDELTRKGVSNSAPPVMVSNVAYGRTVYVKLETTSKSKDVQAAFKALLKNNSVETSG
QYKDIFEESTFTAVVLGGDAKEHNKVVTKDFNEIRNIIKDNAELSFKNPAYPISYTSTFLKDNATAAVHNNTDYIETTTT
EYSSAKMTLDHYGAYVAQFDVSWDEFTFDQNGKEVLTHKTWEGSGKDKTAHYSTVIPLPPNSKNIKIVARECTGLAWEWW
RTIINEQNVPLTNEIKVSIGGTTLYPTATISH

Sequences:

>Translated_512_residues
MIFLNIKKNSKRRKFLTCVLVSLCTLNYSSISFAETQAGHATDITKNASSIDTGIGNLTYNNQEVLAVNGDKVESFVPKE
SINSNGKFVVVEREKKSLTTSPVDISIIDSVANRTYPGAVQLANKAFADNQPSLLVAKRKPLNISIDLPGMRKENTITVQ
NPTYGNVAGAVDDLVSTWNEKYSTTHTLPARMQYTESMVYSKSQIASALNVNAKYLDNSLNIDFNAVANGEKKVMVAAYK
QIFYTVSAELPNNPSDLFDNSVTFDELTRKGVSNSAPPVMVSNVAYGRTVYVKLETTSKSKDVQAAFKALLKNNSVETSG
QYKDIFEESTFTAVVLGGDAKEHNKVVTKDFNEIRNIIKDNAELSFKNPAYPISYTSTFLKDNATAAVHNNTDYIETTTT
EYSSAKMTLDHYGAYVAQFDVSWDEFTFDQNGKEVLTHKTWEGSGKDKTAHYSTVIPLPPNSKNIKIVARECTGLAWEWW
RTIINEQNVPLTNEIKVSIGGTTLYPTATISH
>Mature_512_residues
MIFLNIKKNSKRRKFLTCVLVSLCTLNYSSISFAETQAGHATDITKNASSIDTGIGNLTYNNQEVLAVNGDKVESFVPKE
SINSNGKFVVVEREKKSLTTSPVDISIIDSVANRTYPGAVQLANKAFADNQPSLLVAKRKPLNISIDLPGMRKENTITVQ
NPTYGNVAGAVDDLVSTWNEKYSTTHTLPARMQYTESMVYSKSQIASALNVNAKYLDNSLNIDFNAVANGEKKVMVAAYK
QIFYTVSAELPNNPSDLFDNSVTFDELTRKGVSNSAPPVMVSNVAYGRTVYVKLETTSKSKDVQAAFKALLKNNSVETSG
QYKDIFEESTFTAVVLGGDAKEHNKVVTKDFNEIRNIIKDNAELSFKNPAYPISYTSTFLKDNATAAVHNNTDYIETTTT
EYSSAKMTLDHYGAYVAQFDVSWDEFTFDQNGKEVLTHKTWEGSGKDKTAHYSTVIPLPPNSKNIKIVARECTGLAWEWW
RTIINEQNVPLTNEIKVSIGGTTLYPTATISH

Specific function: Sulfhydryl-activated toxin with hemolytic activity against host red blood cells. Causes cytolysis by forming pores in cholesterol containing host membranes. binding to target membranes, the protein undergoes a major conformation change, leading to its ins

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Host cell membrane; Multi-pass membrane protein (By similarity). Note=Secreted as soluble protein that then inserts into the host cell membrane and forms pores formed by transmembrane beta-strands (By similarity) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiol-activated cytolysin family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001869 [H]

Pfam domain/function: PF01289 Thiol_cytolysin [H]

EC number: NA

Molecular weight: Translated: 56599; Mature: 56599

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00481 THIOL_CYTOLYSINS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFLNIKKNSKRRKFLTCVLVSLCTLNYSSISFAETQAGHATDITKNASSIDTGIGNLTY
CEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEHHCCCCCCCCCCCCCCHHCCCCCCEEE
NNQEVLAVNGDKVESFVPKESINSNGKFVVVEREKKSLTTSPVDISIIDSVANRTYPGAV
CCCEEEEECCCHHHHHCCHHHCCCCCCEEEEECCCCCCCCCCCEEEEEHHHHCCCCCCHH
QLANKAFADNQPSLLVAKRKPLNISIDLPGMRKENTITVQNPTYGNVAGAVDDLVSTWNE
HHHHHHCCCCCCCEEEEECCCCEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
KYSTTHTLPARMQYTESMVYSKSQIASALNVNAKYLDNSLNIDFNAVANGEKKVMVAAYK
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEEECCCCCEEEHHEEE
QIFYTVSAELPNNPSDLFDNSVTFDELTRKGVSNSAPPVMVSNVAYGRTVYVKLETTSKS
EEEEEEEECCCCCHHHHCCCCCCHHHHHHCCCCCCCCCEEEECCCCCEEEEEEEECCCCC
KDVQAAFKALLKNNSVETSGQYKDIFEESTFTAVVLGGDAKEHNKVVTKDFNEIRNIIKD
CHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEEECCCCHHCCCCHHHHHHHHHHHHHC
NAELSFKNPAYPISYTSTFLKDNATAAVHNNTDYIETTTTEYSSAKMTLDHYGAYVAQFD
CCCEEECCCCCCCCHHHHHHCCCCEEEEECCCCEEEEECCCCCCEEEEHHHCCCEEEEEE
VSWDEFTFDQNGKEVLTHKTWEGSGKDKTAHYSTVIPLPPNSKNIKIVARECTGLAWEWW
CCHHHEEECCCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCCHHHHH
RTIINEQNVPLTNEIKVSIGGTTLYPTATISH
HHHHCCCCCCCCCEEEEEECCEEEECEEECCC
>Mature Secondary Structure
MIFLNIKKNSKRRKFLTCVLVSLCTLNYSSISFAETQAGHATDITKNASSIDTGIGNLTY
CEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEHHCCCCCCCCCCCCCCHHCCCCCCEEE
NNQEVLAVNGDKVESFVPKESINSNGKFVVVEREKKSLTTSPVDISIIDSVANRTYPGAV
CCCEEEEECCCHHHHHCCHHHCCCCCCEEEEECCCCCCCCCCCEEEEEHHHHCCCCCCHH
QLANKAFADNQPSLLVAKRKPLNISIDLPGMRKENTITVQNPTYGNVAGAVDDLVSTWNE
HHHHHHCCCCCCCEEEEECCCCEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
KYSTTHTLPARMQYTESMVYSKSQIASALNVNAKYLDNSLNIDFNAVANGEKKVMVAAYK
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEEECCCCCEEEHHEEE
QIFYTVSAELPNNPSDLFDNSVTFDELTRKGVSNSAPPVMVSNVAYGRTVYVKLETTSKS
EEEEEEEECCCCCHHHHCCCCCCHHHHHHCCCCCCCCCEEEECCCCCEEEEEEEECCCCC
KDVQAAFKALLKNNSVETSGQYKDIFEESTFTAVVLGGDAKEHNKVVTKDFNEIRNIIKD
CHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEEECCCCHHCCCCHHHHHHHHHHHHHC
NAELSFKNPAYPISYTSTFLKDNATAAVHNNTDYIETTTTEYSSAKMTLDHYGAYVAQFD
CCCEEECCCCCCCCHHHHHHCCCCEEEEECCCCEEEEECCCCCCEEEEHHHCCCEEEEEE
VSWDEFTFDQNGKEVLTHKTWEGSGKDKTAHYSTVIPLPPNSKNIKIVARECTGLAWEWW
CCHHHEEECCCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCCHHHHH
RTIINEQNVPLTNEIKVSIGGTTLYPTATISH
HHHHCCCCCCCCCEEEEEECCEEEECEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA