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The map label for this gene is pykA [H]

Identifier: 52142214

GI number: 52142214

Start: 3161482

End: 3162540

Strand: Reverse

Name: pykA [H]

Synonym: BCZK3030

Alternate gene names: 52142214

Gene position: 3162540-3161482 (Counterclockwise)

Preceding gene: 52142211

Following gene: 52142215

Centisome position: 59.66

GC content: 36.07

Gene sequence:

>1059_bases
ATGACAATCGATCGAGTTTGTACAATTGGGCCAGCAAGTAATAACAAAGAAACGTTAGCGCAGTTAATAAACAATGGTAT
GAAAATTGTTCGTCTAAATTTATCACATGGTACGCATGAAAGTCATAAAGATATCATTCGTTTAGTCAAATCGTTAGACG
CTTCTATTAAAATTTTAGGCGATGTACAAGGTCCTAAAATAAGGTTAGGTGAAATAAAAGGAGAACAAATTACGCTTCAG
GCGGGAGATTCTTTTATGTTACGTACGCAACCAGGTACAGGGAGTAATAGGGAAGCAAGTATTGATTATGAAGGAATCGC
GAATGATGTGAAAGTTGGAAGTAGAATTTTAATGAATGATGGCGAAGTTGAGTTAATTGTTGAGAAGGTAAGTACGGATA
AAATAGAAACAAAGGTCAAAACAGGTGGTAATATCTCATCACATAAAGGAGTGAACTTACCAGGGGCAATCGTTAGTTTA
CCAGCTATTACAGAGAAAGATAAAAAAGATATTCAGTTTCTTTTAGAAGAGGATGTTGATTTTATTGCGTGTTCTTTCGT
AAGAAAACCTAGTCATATAAAAGAAATACGAGATTTTATACAACAGTATAAAGAAACGTCACCTAATTTAATTGCAAAAA
TAGAAACGATGGAAGCAATCGAGAATTTTCAAGATATATGTAAAGAAGCAGACGGAATTATGATTGCAAGGGGCGATTTA
GGAGTTGAGTTGCCATTTCAATTTATACCGCTCTTACAGAAAATGATGATTCAGGAATGTAATCGAACGAATACATATGT
TATTACAGCGACTCAAATGCTTCAATCTATGGTAGATCATTCTATTCCAACAAGGGCAGAGGTAACTGATGTGTTTCAAG
CTGTACTGGACGGAACGAATGCAGTTATGCTTTCAGCTGAAAGTGCTTCAGGAGAGCATCCGGTTGAAAGTGTGAGTACA
CTACGTCTCGTTTCTGAATTTGCTGTGCATGTGAAGAAAGATGGTCCGTTTGTGATGAAAGATGTACTGGAATTGTTGCA
TAAATCTTTGGATGAGTGA

Upstream 100 bases:

>100_bases
TTTCTTAGTTTCAAAAGCCTACTTGTGTGAGTAGGCTTTTTTTACGTTGAGAGTGTAATGAAAGTTTATGGGAATCCTAA
TGAAAAAGGATGGTGGAAAT

Downstream 100 bases:

>100_bases
AAAATAAAAAGAGGGCGGATATGTATCCGTCCTCGTGTGTTTCATTCTATTCTTGCTCTTGCTCATCCAAAATCTTTTCA
AACGCAGCAGATACTTTTTC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 2 [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MTIDRVCTIGPASNNKETLAQLINNGMKIVRLNLSHGTHESHKDIIRLVKSLDASIKILGDVQGPKIRLGEIKGEQITLQ
AGDSFMLRTQPGTGSNREASIDYEGIANDVKVGSRILMNDGEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVSL
PAITEKDKKDIQFLLEEDVDFIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDICKEADGIMIARGDL
GVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTNAVMLSAESASGEHPVESVST
LRLVSEFAVHVKKDGPFVMKDVLELLHKSLDE

Sequences:

>Translated_352_residues
MTIDRVCTIGPASNNKETLAQLINNGMKIVRLNLSHGTHESHKDIIRLVKSLDASIKILGDVQGPKIRLGEIKGEQITLQ
AGDSFMLRTQPGTGSNREASIDYEGIANDVKVGSRILMNDGEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVSL
PAITEKDKKDIQFLLEEDVDFIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDICKEADGIMIARGDL
GVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTNAVMLSAESASGEHPVESVST
LRLVSEFAVHVKKDGPFVMKDVLELLHKSLDE
>Mature_351_residues
TIDRVCTIGPASNNKETLAQLINNGMKIVRLNLSHGTHESHKDIIRLVKSLDASIKILGDVQGPKIRLGEIKGEQITLQA
GDSFMLRTQPGTGSNREASIDYEGIANDVKVGSRILMNDGEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVSLP
AITEKDKKDIQFLLEEDVDFIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDICKEADGIMIARGDLG
VELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTNAVMLSAESASGEHPVESVSTL
RLVSEFAVHVKKDGPFVMKDVLELLHKSLDE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=339, Percent_Identity=42.7728613569322, Blast_Score=272, Evalue=3e-73,
Organism=Homo sapiens, GI33286420, Length=339, Percent_Identity=42.7728613569322, Blast_Score=272, Evalue=3e-73,
Organism=Homo sapiens, GI33286418, Length=339, Percent_Identity=42.7728613569322, Blast_Score=272, Evalue=3e-73,
Organism=Homo sapiens, GI10835121, Length=329, Percent_Identity=41.6413373860182, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI32967597, Length=329, Percent_Identity=41.6413373860182, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI310128732, Length=283, Percent_Identity=41.3427561837456, Blast_Score=211, Evalue=7e-55,
Organism=Homo sapiens, GI310128730, Length=283, Percent_Identity=41.3427561837456, Blast_Score=211, Evalue=7e-55,
Organism=Homo sapiens, GI310128736, Length=231, Percent_Identity=38.961038961039, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI310128734, Length=231, Percent_Identity=38.961038961039, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=37.2093023255814, Blast_Score=138, Evalue=7e-33,
Organism=Escherichia coli, GI1787965, Length=327, Percent_Identity=43.1192660550459, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1788160, Length=328, Percent_Identity=41.1585365853659, Blast_Score=231, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI71984413, Length=343, Percent_Identity=44.8979591836735, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI17506829, Length=343, Percent_Identity=44.8979591836735, Blast_Score=270, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI71984406, Length=343, Percent_Identity=44.8979591836735, Blast_Score=270, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI17506831, Length=343, Percent_Identity=44.8979591836735, Blast_Score=270, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI17544584, Length=350, Percent_Identity=40.5714285714286, Blast_Score=258, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6319279, Length=327, Percent_Identity=38.5321100917431, Blast_Score=217, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6324923, Length=326, Percent_Identity=36.5030674846626, Blast_Score=199, Evalue=5e-52,
Organism=Drosophila melanogaster, GI24648964, Length=332, Percent_Identity=44.2771084337349, Blast_Score=269, Evalue=2e-72,
Organism=Drosophila melanogaster, GI28571814, Length=332, Percent_Identity=44.2771084337349, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24648966, Length=312, Percent_Identity=40.7051282051282, Blast_Score=245, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24581235, Length=328, Percent_Identity=34.7560975609756, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24646914, Length=172, Percent_Identity=40.6976744186046, Blast_Score=138, Evalue=5e-33,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 38824; Mature: 38693

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDRVCTIGPASNNKETLAQLINNGMKIVRLNLSHGTHESHKDIIRLVKSLDASIKILG
CCCCCEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEE
DVQGPKIRLGEIKGEQITLQAGDSFMLRTQPGTGSNREASIDYEGIANDVKVGSRILMND
CCCCCEEEEEECCCCEEEEEECCEEEEEECCCCCCCCCCCCCHHHCCCHHHHCCEEEECC
GEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVSLPAITEKDKKDIQFLLEEDVD
CCEEEEEEHHCCHHHHHHCCCCCCCCCCCCCCCCHHHEECCCCCCCCHHHHHHHHHHCCH
FIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDICKEADGIMIARGDL
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEEECCC
GVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTN
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
AVMLSAESASGEHPVESVSTLRLVSEFAVHVKKDGPFVMKDVLELLHKSLDE
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIDRVCTIGPASNNKETLAQLINNGMKIVRLNLSHGTHESHKDIIRLVKSLDASIKILG
CCCCEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEE
DVQGPKIRLGEIKGEQITLQAGDSFMLRTQPGTGSNREASIDYEGIANDVKVGSRILMND
CCCCCEEEEEECCCCEEEEEECCEEEEEECCCCCCCCCCCCCHHHCCCHHHHCCEEEECC
GEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVSLPAITEKDKKDIQFLLEEDVD
CCEEEEEEHHCCHHHHHHCCCCCCCCCCCCCCCCHHHEECCCCCCCCHHHHHHHHHHCCH
FIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDICKEADGIMIARGDL
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEEECCC
GVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTN
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
AVMLSAESASGEHPVESVSTLRLVSEFAVHVKKDGPFVMKDVLELLHKSLDE
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]