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The map label for this gene is galE [H]

Identifier: 52142170

GI number: 52142170

Start: 3199644

End: 3200567

Strand: Direct

Name: galE [H]

Synonym: BCZK3072

Alternate gene names: 52142170

Gene position: 3199644-3200567 (Clockwise)

Preceding gene: 52142171

Following gene: 52142166

Centisome position: 60.36

GC content: 38.64

Gene sequence:

>924_bases
ATGAAAAAAATCCTTGTAACAGGCGGTAGCGGATGGATTGGTAAATATGTTGTACATTTTCTTATACAAAAGGGCTATGA
AGTGCACGCCACTTACAATACAAATCAGCCTTCTCACCTTTCTTGCTATTGGCATAAAGTTAACTTACTTTGCGATGAAG
AAATAGAAAAACTCATTTACGACATAAAACCTAGTCATCTCATTCATTTAGCTTGGGAAGCAGTGCCTCCAGCATGCTAC
GTATCCATTAATAATTACTATTGGCTAACCTCCAGCATCTCATTGATTCAACACTTCACAACATGCGGCGGAAAGCGAGT
CGTCGTTGCCGGTACTGGCGCAGAGTATGAATGGTTTAACGGTGTATTATTTGAAGATTCACAGCTTCTTTCTTATAAAA
CCCCGTATTCATTATGTAAAAATGCACTACACTCCTGGCTCCAAACGTACGCCAAGCAAACCGGTCTCAGCATGTGTTGG
GGCCGCATTTTTCATATGTATGGCCCTCACGAACAAGGGAATCGGCTCGTTTCTAACATTATCACTTCTTTATTAAAAAA
TGAGGAAGCACTATGTACACATGGAAAACAATCAAGAGATTTCCTCCATGTGAGCGACGTCGCTGATGCTCTCGTAACAG
TATTAGAACATGGCGTTACAGACACAATCAATATCGCTTCTGGTCAATCTGTACAAATTAAAGAGTTAGCTTCTATCATC
GCTAAAAAGATAGGAAAAGAACATCTAATTAAACTAGGTGCAATACCTTTCTCTAAAGATGAACCTTTATTTGTCGGGGT
TAATGTAGAACGTTTAAAAACTGAAGTAAACTGGAAACCAACATACGATCTAAATACAGGAATCGAAGAAACAATTTTAT
GGTGGGAATCATTTATAAAAAAGCATAACGGTACGCATCACTAA

Upstream 100 bases:

>100_bases
CTTGGAATTTGAAAGATGAAATTATGAAAGAATGCTCTTTTATTCGTGAATGGGGCGGCAAATTTTTAGTAACAATACCA
GAAGTTGAGGTAATTGAGCC

Downstream 100 bases:

>100_bases
AACGTAAATCCTTCGTTCCTCTCGCTAATTCCTTATACCATTCTTCTAATCTTTCCGGCGTAATAATATGATGTGTACCC
ATTCCCCCATGCTTACTAGC

Product: UDP-glucose 4-epimerase

Products: UDPgalactose

Alternate protein names: O4 antigen [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKKILVTGGSGWIGKYVVHFLIQKGYEVHATYNTNQPSHLSCYWHKVNLLCDEEIEKLIYDIKPSHLIHLAWEAVPPACY
VSINNYYWLTSSISLIQHFTTCGGKRVVVAGTGAEYEWFNGVLFEDSQLLSYKTPYSLCKNALHSWLQTYAKQTGLSMCW
GRIFHMYGPHEQGNRLVSNIITSLLKNEEALCTHGKQSRDFLHVSDVADALVTVLEHGVTDTINIASGQSVQIKELASII
AKKIGKEHLIKLGAIPFSKDEPLFVGVNVERLKTEVNWKPTYDLNTGIEETILWWESFIKKHNGTHH

Sequences:

>Translated_307_residues
MKKILVTGGSGWIGKYVVHFLIQKGYEVHATYNTNQPSHLSCYWHKVNLLCDEEIEKLIYDIKPSHLIHLAWEAVPPACY
VSINNYYWLTSSISLIQHFTTCGGKRVVVAGTGAEYEWFNGVLFEDSQLLSYKTPYSLCKNALHSWLQTYAKQTGLSMCW
GRIFHMYGPHEQGNRLVSNIITSLLKNEEALCTHGKQSRDFLHVSDVADALVTVLEHGVTDTINIASGQSVQIKELASII
AKKIGKEHLIKLGAIPFSKDEPLFVGVNVERLKTEVNWKPTYDLNTGIEETILWWESFIKKHNGTHH
>Mature_307_residues
MKKILVTGGSGWIGKYVVHFLIQKGYEVHATYNTNQPSHLSCYWHKVNLLCDEEIEKLIYDIKPSHLIHLAWEAVPPACY
VSINNYYWLTSSISLIQHFTTCGGKRVVVAGTGAEYEWFNGVLFEDSQLLSYKTPYSLCKNALHSWLQTYAKQTGLSMCW
GRIFHMYGPHEQGNRLVSNIITSLLKNEEALCTHGKQSRDFLHVSDVADALVTVLEHGVTDTINIASGQSVQIKELASII
AKKIGKEHLIKLGAIPFSKDEPLFVGVNVERLKTEVNWKPTYDLNTGIEETILWWESFIKKHNGTHH

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI42516563, Length=312, Percent_Identity=25.6410256410256, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI7657641, Length=323, Percent_Identity=24.4582043343653, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI48994969, Length=337, Percent_Identity=24.9258160237389, Blast_Score=71, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21356223, Length=320, Percent_Identity=26.5625, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 34814; Mature: 34814

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILVTGGSGWIGKYVVHFLIQKGYEVHATYNTNQPSHLSCYWHKVNLLCDEEIEKLIY
CCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEEEEEEEHHHHHHHHH
DIKPSHLIHLAWEAVPPACYVSINNYYWLTSSISLIQHFTTCGGKRVVVAGTGAEYEWFN
HCCCCCEEEEEECCCCCEEEEEECCEEEEEHHHHHHHHHHHCCCCEEEEEECCCCCHHHC
GVLFEDSQLLSYKTPYSLCKNALHSWLQTYAKQTGLSMCWGRIFHMYGPHEQGNRLVSNI
CEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHH
ITSLLKNEEALCTHGKQSRDFLHVSDVADALVTVLEHGVTDTINIASGQSVQIKELASII
HHHHHCCCHHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHHH
AKKIGKEHLIKLGAIPFSKDEPLFVGVNVERLKTEVNWKPTYDLNTGIEETILWWESFIK
HHHHCHHHHHEECCCCCCCCCCEEEECCHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHH
KHNGTHH
HCCCCCC
>Mature Secondary Structure
MKKILVTGGSGWIGKYVVHFLIQKGYEVHATYNTNQPSHLSCYWHKVNLLCDEEIEKLIY
CCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEEEEEEEHHHHHHHHH
DIKPSHLIHLAWEAVPPACYVSINNYYWLTSSISLIQHFTTCGGKRVVVAGTGAEYEWFN
HCCCCCEEEEEECCCCCEEEEEECCEEEEEHHHHHHHHHHHCCCCEEEEEECCCCCHHHC
GVLFEDSQLLSYKTPYSLCKNALHSWLQTYAKQTGLSMCWGRIFHMYGPHEQGNRLVSNI
CEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHH
ITSLLKNEEALCTHGKQSRDFLHVSDVADALVTVLEHGVTDTINIASGQSVQIKELASII
HHHHHCCCHHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHHH
AKKIGKEHLIKLGAIPFSKDEPLFVGVNVERLKTEVNWKPTYDLNTGIEETILWWESFIK
HHHHCHHHHHEECCCCCCCCCCEEEECCHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHH
KHNGTHH
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2793832; 1710759; 11677609 [H]