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The map label for this gene is adh [C]

Identifier: 52142158

GI number: 52142158

Start: 3214979

End: 3215932

Strand: Reverse

Name: adh [C]

Synonym: BCZK3085

Alternate gene names: 52142158

Gene position: 3215932-3214979 (Counterclockwise)

Preceding gene: 52142156

Following gene: 52142157

Centisome position: 60.67

GC content: 39.73

Gene sequence:

>954_bases
ATGAAAGCGATAGGACTTATGCAATATGGAGATAAAAGTGTACTACAAGAGATTGAAATGCAGACACCGCTATTAGGAGA
CACCGATGTACTAATTGAAGTATATGCAGCTGGTATAAATCCCGTGGATTGGAAAATACGTGAAGGTTTACTGCAAGACG
TAATTTCTTATGACTTCCCGCTCGTTTTAGGATGGGATGTTGCAGGTGTAGTTGCTGCTATAGGGCCAAACGTAACAGCA
TGTAAAGTGGGGGATGAAGTATATAGTCGTCCGGATATTGAACGAAACGGTGCTTATGCAGAGTACGTGGCTGTAAATGA
GAAATATGTGGCGAAAAAACCGAGGAATTTATCTTTTGAAGAGGCGGCATCAATCCCTCTTGTAGGTTTGACAAGTTGGC
AAAGCTTAGTAAAGTTCGCAAATGTTCAAAAAGGCAATAAGGTTTTAATTCATGCTGGATCTGGCGGGATTGGTACGTTT
GCTATTCAGCTAGCCAAAAGCTTCGGTGCACATGTTGCAACTACGACTAGTACGAAAAACATGCAGTTTGTAAAGGATCT
AGGCGCTGATACTGTCGTCGATTATAAAACGGAAGACTTTTCTTCGCTTTTGCATAATTACAATATTGTATTTGATGTGT
TAGGCGGAGATGTACTAAAGAATAGTTATAAAGTGCTTGCACCAAATGGAAAGTTAGCATCTATTTATGGTCCAAAAGGT
ATGGAAATTCCGCAAACCGAGATATCAAGAGAGAAAAATATTGAGAGCGACCATATATTTACTGAACCTAACGGATATGA
ACTTTCTCTTATTACAGAATTAATTGAGGGTGGGAAGATTAAACCCGTTGTCACGCATGTGTTGCCTTTACATGTAGAAG
GCGTAAAAAAAGCACACCACATAAGTGAGTCAGAGCGTGCTCGTGGCAAAATAGTATTGAAGAAACATTGGTAA

Upstream 100 bases:

>100_bases
CTTGCTCTATTTGATGGTCAACGTCCAGCTGACGAAGTATTGGATGAGCTAGGAGAAGAAGTACTACCTCATTTCCCAGC
ATTGTAAAGGAGTGAAAAGA

Downstream 100 bases:

>100_bases
AGTATGAGGTTTTAGAGACAGTATTGTGCTAAGTAGCGAATGAGTTTATATATTCTATTCTAGCAATTGTTTTAAAAGGG
AGAGAACAAATGGATTCAAT

Product: bifunctional protein: zinc-containing alcohol dehydrogenase; quinone oxidoreductase ( NADPH:quinone reductase)

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKAIGLMQYGDKSVLQEIEMQTPLLGDTDVLIEVYAAGINPVDWKIREGLLQDVISYDFPLVLGWDVAGVVAAIGPNVTA
CKVGDEVYSRPDIERNGAYAEYVAVNEKYVAKKPRNLSFEEAASIPLVGLTSWQSLVKFANVQKGNKVLIHAGSGGIGTF
AIQLAKSFGAHVATTTSTKNMQFVKDLGADTVVDYKTEDFSSLLHNYNIVFDVLGGDVLKNSYKVLAPNGKLASIYGPKG
MEIPQTEISREKNIESDHIFTEPNGYELSLITELIEGGKIKPVVTHVLPLHVEGVKKAHHISESERARGKIVLKKHW

Sequences:

>Translated_317_residues
MKAIGLMQYGDKSVLQEIEMQTPLLGDTDVLIEVYAAGINPVDWKIREGLLQDVISYDFPLVLGWDVAGVVAAIGPNVTA
CKVGDEVYSRPDIERNGAYAEYVAVNEKYVAKKPRNLSFEEAASIPLVGLTSWQSLVKFANVQKGNKVLIHAGSGGIGTF
AIQLAKSFGAHVATTTSTKNMQFVKDLGADTVVDYKTEDFSSLLHNYNIVFDVLGGDVLKNSYKVLAPNGKLASIYGPKG
MEIPQTEISREKNIESDHIFTEPNGYELSLITELIEGGKIKPVVTHVLPLHVEGVKKAHHISESERARGKIVLKKHW
>Mature_317_residues
MKAIGLMQYGDKSVLQEIEMQTPLLGDTDVLIEVYAAGINPVDWKIREGLLQDVISYDFPLVLGWDVAGVVAAIGPNVTA
CKVGDEVYSRPDIERNGAYAEYVAVNEKYVAKKPRNLSFEEAASIPLVGLTSWQSLVKFANVQKGNKVLIHAGSGGIGTF
AIQLAKSFGAHVATTTSTKNMQFVKDLGADTVVDYKTEDFSSLLHNYNIVFDVLGGDVLKNSYKVLAPNGKLASIYGPKG
MEIPQTEISREKNIESDHIFTEPNGYELSLITELIEGGKIKPVVTHVLPLHVEGVKKAHHISESERARGKIVLKKHW

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=358, Percent_Identity=30.7262569832402, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI194239674, Length=331, Percent_Identity=30.8157099697885, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI13236495, Length=331, Percent_Identity=30.8157099697885, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI22538446, Length=322, Percent_Identity=30.4347826086957, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI22538444, Length=322, Percent_Identity=30.4347826086957, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI194239676, Length=321, Percent_Identity=30.5295950155763, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI24308257, Length=251, Percent_Identity=30.2788844621514, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI18379349, Length=348, Percent_Identity=27.5862068965517, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI28557745, Length=234, Percent_Identity=30.3418803418803, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI90669511, Length=167, Percent_Identity=31.1377245508982, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1790485, Length=331, Percent_Identity=28.3987915407855, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17507255, Length=242, Percent_Identity=34.7107438016529, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI71987554, Length=339, Percent_Identity=30.6784660766962, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17536829, Length=197, Percent_Identity=27.4111675126904, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6319520, Length=267, Percent_Identity=28.0898876404494, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324195, Length=269, Percent_Identity=27.8810408921933, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319945, Length=270, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581341, Length=242, Percent_Identity=30.1652892561983, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24581339, Length=242, Percent_Identity=30.1652892561983, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24581345, Length=262, Percent_Identity=25.5725190839695, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19920632, Length=238, Percent_Identity=26.4705882352941, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI221330659, Length=238, Percent_Identity=26.4705882352941, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR014182
- InterPro:   IPR011032
- InterPro:   IPR016040
- InterPro:   IPR002364 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 34677; Mature: 34677

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIGLMQYGDKSVLQEIEMQTPLLGDTDVLIEVYAAGINPVDWKIREGLLQDVISYDFP
CCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
LVLGWDVAGVVAAIGPNVTACKVGDEVYSRPDIERNGAYAEYVAVNEKYVAKKPRNLSFE
EEECCCHHHHHHHHCCCCEEEECCHHHHCCCCCCCCCCEEEEEEECCHHHHCCCCCCCHH
EAASIPLVGLTSWQSLVKFANVQKGNKVLIHAGSGGIGTFAIQLAKSFGAHVATTTSTKN
HHCCCCEEEHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCC
MQFVKDLGADTVVDYKTEDFSSLLHNYNIVFDVLGGDVLKNSYKVLAPNGKLASIYGPKG
HHHHHHCCCCCEEECCCHHHHHHHHCCCEEEEECCCHHHHCCCEEECCCCCEEEEECCCC
MEIPQTEISREKNIESDHIFTEPNGYELSLITELIEGGKIKPVVTHVLPLHVEGVKKAHH
CCCCHHHHHHHHCCCCCEEEECCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ISESERARGKIVLKKHW
CCHHHHCCCEEEEEECC
>Mature Secondary Structure
MKAIGLMQYGDKSVLQEIEMQTPLLGDTDVLIEVYAAGINPVDWKIREGLLQDVISYDFP
CCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
LVLGWDVAGVVAAIGPNVTACKVGDEVYSRPDIERNGAYAEYVAVNEKYVAKKPRNLSFE
EEECCCHHHHHHHHCCCCEEEECCHHHHCCCCCCCCCCEEEEEEECCHHHHCCCCCCCHH
EAASIPLVGLTSWQSLVKFANVQKGNKVLIHAGSGGIGTFAIQLAKSFGAHVATTTSTKN
HHCCCCEEEHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCC
MQFVKDLGADTVVDYKTEDFSSLLHNYNIVFDVLGGDVLKNSYKVLAPNGKLASIYGPKG
HHHHHHCCCCCEEECCCHHHHHHHHCCCEEEEECCCHHHHCCCEEECCCCCEEEEECCCC
MEIPQTEISREKNIESDHIFTEPNGYELSLITELIEGGKIKPVVTHVLPLHVEGVKKAHH
CCCCHHHHHHHHCCCCCEEEECCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ISESERARGKIVLKKHW
CCHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA