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The map label for this gene is yhjG [H]

Identifier: 52142112

GI number: 52142112

Start: 3260393

End: 3261844

Strand: Direct

Name: yhjG [H]

Synonym: BCZK3131

Alternate gene names: 52142112

Gene position: 3260393-3261844 (Clockwise)

Preceding gene: 52142111

Following gene: 52142097

Centisome position: 61.51

GC content: 33.4

Gene sequence:

>1452_bases
ATGGAAAATTATTATGATGTAGCGGTAGTTGGTGGTGGCCCAGTTGGACTTATGACAGCATGTGAATTAGCTATTCAAGG
TATAAACGTTGTCGTTTTGGAGAGAAGAGTGGATCGCATACGAAATTCTAGAGCACTAACACTACACCCCCGCTCACTAG
AATTAATGGCTATGAGAGGCATTGAAGATCGATTTTTATCTAGAGGAAAGAAATTAGATACAAGTCATTTTGCTGCCTTA
GATACAAGATTAAACTTTAAAAATTTAGATACTGACTTTCCGTATACACTTTTTATCCCCCAATCTATTACAGAAAAAAT
CTTAGAAGAACGTGCATTGGAATTAGGTGTAGACATTAAACGTGGGCATACTCTGAAAGACTTAAAACAGTATGAAACAT
TCGTACAACTAGAAATAGAACAAAGAGAAACAATATATAAAATAGAAGTTCCATATGTAGTAGGAGCTGATGGCGCAAGA
AGCATGGTTCGTGAATTATCAAATATACCGTTTAATGGAACAAATACAACAACTACTGCTATATTAGGTGATGTTATATT
AGAAGATATACCAGATCCTCCATTTTTATCTACTTCTAATATAAATGGGATATTAATGATGGTGCCTATTGCACCTAATT
TATTTCGTTTTGCAATTGTTGATCCTAAGAATCAGACAATACCTGTTCATGTTGCTGTTACAGAAAAGGAGTTTATAGAT
AGTATTTATAGGATTACAGGTAAACATATTCAAATTAAGAGCACAGTATGGCTTTCTCGCTTTGGGAACGCAACCAAACT
AGCAAATACTTATCAACATAAACGTTTATTCTTAGCAGGGGATGCAGCACATATTCATTTCCCTGCTGGAGGACAAGGTT
TGAATGTAGGTCTACAAGATGCTTTTAATATAGGATGGAAATTAGGACTCGCAATAAAACAAAAAAAATATGAAAGTCTA
TTAGAAAGTTATCATATTGAACGACATGCTGTGGGAAAACAATTTTTCAAGGAAGTACAAGCGCAAGAACAATTAATGAT
AAACTTTTCAAACGCAGGAATGGAATTAAGGGAGTTATTTTCCCACTTACTTACATACCCCGAGATAAATAACGATTTAT
CTGAAAGAGTAAGTGGTTTAGGGGTTAGGTATTCGTCGATGCACGATACAGAAAAGCATCTATTAATAGGAAAAAGATGT
ACAAATCTCACTATTTCTTCTCAAAATAAGAAAACTTATAAACTCTTTGAATTAATGCAGGATGGTAAATTTATTTTATT
AATACACCCTTCTTATATAGATAAGATAAATAAAATGAATATTGATTCGCACATAAAGGTAATTGTAGGAGAAATACAAG
ATTCCAAGTTAAAAAATATAGATACCATTTTGATTCGACCAGATGGTCACATAGCCGACATTGAGTATAACAAGGTAGAT
AACTGCGAATAG

Upstream 100 bases:

>100_bases
AATGAAGTATAATTATATACGTACAATAGATAGCATTTATAAATCTATTTACATTTTTTCGCATTAGCTCTTAATCACTT
AAATAAATGGAGGGCATGAT

Downstream 100 bases:

>100_bases
TCCAGAAAAATGATTATCAATTGTACTAGTGATACAAGATAAATTCACTTTTAAAAACGAAAAGAATCCTATTTATTTAG
GATTCTTTTCGTTTTGCCCA

Product: PheA/TfdB family FAD-binding monooxygenase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MENYYDVAVVGGGPVGLMTACELAIQGINVVVLERRVDRIRNSRALTLHPRSLELMAMRGIEDRFLSRGKKLDTSHFAAL
DTRLNFKNLDTDFPYTLFIPQSITEKILEERALELGVDIKRGHTLKDLKQYETFVQLEIEQRETIYKIEVPYVVGADGAR
SMVRELSNIPFNGTNTTTTAILGDVILEDIPDPPFLSTSNINGILMMVPIAPNLFRFAIVDPKNQTIPVHVAVTEKEFID
SIYRITGKHIQIKSTVWLSRFGNATKLANTYQHKRLFLAGDAAHIHFPAGGQGLNVGLQDAFNIGWKLGLAIKQKKYESL
LESYHIERHAVGKQFFKEVQAQEQLMINFSNAGMELRELFSHLLTYPEINNDLSERVSGLGVRYSSMHDTEKHLLIGKRC
TNLTISSQNKKTYKLFELMQDGKFILLIHPSYIDKINKMNIDSHIKVIVGEIQDSKLKNIDTILIRPDGHIADIEYNKVD
NCE

Sequences:

>Translated_483_residues
MENYYDVAVVGGGPVGLMTACELAIQGINVVVLERRVDRIRNSRALTLHPRSLELMAMRGIEDRFLSRGKKLDTSHFAAL
DTRLNFKNLDTDFPYTLFIPQSITEKILEERALELGVDIKRGHTLKDLKQYETFVQLEIEQRETIYKIEVPYVVGADGAR
SMVRELSNIPFNGTNTTTTAILGDVILEDIPDPPFLSTSNINGILMMVPIAPNLFRFAIVDPKNQTIPVHVAVTEKEFID
SIYRITGKHIQIKSTVWLSRFGNATKLANTYQHKRLFLAGDAAHIHFPAGGQGLNVGLQDAFNIGWKLGLAIKQKKYESL
LESYHIERHAVGKQFFKEVQAQEQLMINFSNAGMELRELFSHLLTYPEINNDLSERVSGLGVRYSSMHDTEKHLLIGKRC
TNLTISSQNKKTYKLFELMQDGKFILLIHPSYIDKINKMNIDSHIKVIVGEIQDSKLKNIDTILIRPDGHIADIEYNKVD
NCE
>Mature_483_residues
MENYYDVAVVGGGPVGLMTACELAIQGINVVVLERRVDRIRNSRALTLHPRSLELMAMRGIEDRFLSRGKKLDTSHFAAL
DTRLNFKNLDTDFPYTLFIPQSITEKILEERALELGVDIKRGHTLKDLKQYETFVQLEIEQRETIYKIEVPYVVGADGAR
SMVRELSNIPFNGTNTTTTAILGDVILEDIPDPPFLSTSNINGILMMVPIAPNLFRFAIVDPKNQTIPVHVAVTEKEFID
SIYRITGKHIQIKSTVWLSRFGNATKLANTYQHKRLFLAGDAAHIHFPAGGQGLNVGLQDAFNIGWKLGLAIKQKKYESL
LESYHIERHAVGKQFFKEVQAQEQLMINFSNAGMELRELFSHLLTYPEINNDLSERVSGLGVRYSSMHDTEKHLLIGKRC
TNLTISSQNKKTYKLFELMQDGKFILLIHPSYIDKINKMNIDSHIKVIVGEIQDSKLKNIDTILIRPDGHIADIEYNKVD
NCE

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=373, Percent_Identity=26.8096514745308, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 54767; Mature: 54767

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENYYDVAVVGGGPVGLMTACELAIQGINVVVLERRVDRIRNSRALTLHPRSLELMAMRG
CCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEHHHHHHHCCCCEEEECCCCEEEEECCC
IEDRFLSRGKKLDTSHFAALDTRLNFKNLDTDFPYTLFIPQSITEKILEERALELGVDIK
CHHHHHHCCCCCCCHHHEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCC
RGHTLKDLKQYETFVQLEIEQRETIYKIEVPYVVGADGARSMVRELSNIPFNGTNTTTTA
CCCCHHHHHHHHHEEEEEEECCCEEEEEEECEEECCCCHHHHHHHHHCCCCCCCCCHHHH
ILGDVILEDIPDPPFLSTSNINGILMMVPIAPNLFRFAIVDPKNQTIPVHVAVTEKEFID
HHHHHHHHCCCCCCCEECCCCCEEEEEEECCCCEEEEEEECCCCCEEEEEEEECHHHHHH
SIYRITGKHIQIKSTVWLSRFGNATKLANTYQHKRLFLAGDAAHIHFPAGGQGLNVGLQD
HHHHHCCCEEEEEEEEEHHHCCCHHHHHHHHHHCEEEEECCCEEEEECCCCCCCCCCHHH
AFNIGWKLGLAIKQKKYESLLESYHIERHAVGKQFFKEVQAQEQLMINFSNAGMELRELF
HHCCCEEEEHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHH
SHLLTYPEINNDLSERVSGLGVRYSSMHDTEKHLLIGKRCTNLTISSQNKKTYKLFELMQ
HHHHCCCCCCCCHHHHHHHCCEEECCCCCCCHHEEEECCCCEEEEECCCCHHHHHHHHHH
DGKFILLIHPSYIDKINKMNIDSHIKVIVGEIQDSKLKNIDTILIRPDGHIADIEYNKVD
CCCEEEEECCHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEEECCCCCEEEEECCCCC
NCE
CCC
>Mature Secondary Structure
MENYYDVAVVGGGPVGLMTACELAIQGINVVVLERRVDRIRNSRALTLHPRSLELMAMRG
CCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEHHHHHHHCCCCEEEECCCCEEEEECCC
IEDRFLSRGKKLDTSHFAALDTRLNFKNLDTDFPYTLFIPQSITEKILEERALELGVDIK
CHHHHHHCCCCCCCHHHEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCC
RGHTLKDLKQYETFVQLEIEQRETIYKIEVPYVVGADGARSMVRELSNIPFNGTNTTTTA
CCCCHHHHHHHHHEEEEEEECCCEEEEEEECEEECCCCHHHHHHHHHCCCCCCCCCHHHH
ILGDVILEDIPDPPFLSTSNINGILMMVPIAPNLFRFAIVDPKNQTIPVHVAVTEKEFID
HHHHHHHHCCCCCCCEECCCCCEEEEEEECCCCEEEEEEECCCCCEEEEEEEECHHHHHH
SIYRITGKHIQIKSTVWLSRFGNATKLANTYQHKRLFLAGDAAHIHFPAGGQGLNVGLQD
HHHHHCCCEEEEEEEEEHHHCCCHHHHHHHHHHCEEEEECCCEEEEECCCCCCCCCCHHH
AFNIGWKLGLAIKQKKYESLLESYHIERHAVGKQFFKEVQAQEQLMINFSNAGMELRELF
HHCCCEEEEHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHH
SHLLTYPEINNDLSERVSGLGVRYSSMHDTEKHLLIGKRCTNLTISSQNKKTYKLFELMQ
HHHHCCCCCCCCHHHHHHHCCEEECCCCCCCHHEEEECCCCEEEEECCCCHHHHHHHHHH
DGKFILLIHPSYIDKINKMNIDSHIKVIVGEIQDSKLKNIDTILIRPDGHIADIEYNKVD
CCCEEEEECCHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEEECCCCCEEEEECCCCC
NCE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]