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The map label for this gene is glcD [H]

Identifier: 52142013

GI number: 52142013

Start: 3349194

End: 3350585

Strand: Reverse

Name: glcD [H]

Synonym: BCZK3230

Alternate gene names: 52142013

Gene position: 3350585-3349194 (Counterclockwise)

Preceding gene: 52142012

Following gene: 52142014

Centisome position: 63.21

GC content: 38.07

Gene sequence:

>1392_bases
ATGGAAATAACAGTAGAGCGTTTAGTAAATGAATTAAAGGGTGTACTTCCCGAAGATCGAGTAGTAATTAATACGACAGT
AAGAGAGTTACATAGTAAAGATGAATCTTATCATGCCAGTAGTTTACCTGATGTAGTAGTTTTTCCGAGAACGACAGAAG
AGGTTAGCACGATAATGAAAATAGCGAGTGAGCACGGGACAGCTGTCGTTCCGTTTGGGGTAGGTTCTAGTTTAGAAGGA
CATGTAATCCCTTATGAAAAGGGAATTACAGTTGATTTTTCATTAATGAACAAAATTCTTGAAATAAGAGAGAAAGATTT
TCTGGTGAAAGTACAGCCAGGAGTGACTCGTTCTCAGCTCAATAAAGAATTGAAGAAATACGGTTTGTTTTTTAGTGTAG
ATCCAGGGGCAGATGCAACGTTAGGTGGAATGGCTGCTACAAATGCGAGTGGAACGACAGCCGTGAAGTATGGTGTTATG
CGTGATCAAGTTCGTGATTTAGAAGTCGTTCTTGCTAATGGAGAAGTAATACATACTGGGAATTTAGCAGCGAAGTCATC
ATCAGGTTATCATTTAAATGGTATTTTTGTAGGTTCAGAAGGGACACTTGGATGTTTTACCGAATTAACATTAAAAGTAT
ACGGCATACCAGAGCATGTTATGGCTGCAAGAGCATCGTTTCCAGCTATAAATGATGCAGTAGAAGCTGTTATTAACATA
TTGCAAGCTGGTATTCCGATTGCGAGAATTGAACTCGTTGATGAACTATCTATGAAACAAGTCAATCATTATAGTGAAAC
AAGTTACAGAGAAGAACCTACATTGTTTTTAGAGTTTCACGGTAATGAAGCAGGGTTAAAGCAAGATGTTGAATTTACGA
AGGAAATTGTTTTTGATCATAAATGTAAAGAAGTTGCTTTTGAAACGGAAACTGCAGCGAGAAATAAATTATGGGATGCA
CGGCATAATCTAGCATATGCTTACGTTCATAGCTATCCTGGTAAAAAGCTTATGAGTACAGACGTATGTGTACCTATTTC
AGAATTAGCTGGAGCTATCCAACAAGCGAAAGAGACATTAGAAAAGAATGGTCTTGTTGGTGGTATTCTTGGTCATGTAG
GAGACGGGAATTTCCACGTGCTCTTAATGGTTGATCCAAACGATAAAGAAGAAGTGGAAAAAGCGGATGAAATAAATGAA
AGTATCGTTCTATATGCTCTTAAACGAGGAGGAACATGTACAGGGGAACATGGCGTTGGTATTGGAAAACGGAAGTATCA
GGAAGAAGAACATGGGGCGGCATTATTCGTAATGGAAAAAATAAAGAAAGCTCTTGATCCGCAAAATATTTTGAATCCGA
ATAAGGTGTTTCAATTAAGGGATAGGAGCTGA

Upstream 100 bases:

>100_bases
GTATATGTAAAACTTGAATTATTTAATTTTTCGGATTATTATGTATAAAACGCTTAACATATTTTGTAATTCGAATTTAT
AGAGAGTAGGAGAGTGTACG

Downstream 100 bases:

>100_bases
TCGGGTGAAAGTACTAGAAGCGATAAGTAATATAGCTGGAAAGTATTTTGCATTTTGGGTTATTTGTATTGCAACTATTG
CGTATTTTATTCCCGCTCCA

Product: glycolate oxidase, subunit D

Products: pyruvate; ferrocytochrome c

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MEITVERLVNELKGVLPEDRVVINTTVRELHSKDESYHASSLPDVVVFPRTTEEVSTIMKIASEHGTAVVPFGVGSSLEG
HVIPYEKGITVDFSLMNKILEIREKDFLVKVQPGVTRSQLNKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVM
RDQVRDLEVVLANGEVIHTGNLAAKSSSGYHLNGIFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPAINDAVEAVINI
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDHKCKEVAFETETAARNKLWDA
RHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQQAKETLEKNGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADEINE
SIVLYALKRGGTCTGEHGVGIGKRKYQEEEHGAALFVMEKIKKALDPQNILNPNKVFQLRDRS

Sequences:

>Translated_463_residues
MEITVERLVNELKGVLPEDRVVINTTVRELHSKDESYHASSLPDVVVFPRTTEEVSTIMKIASEHGTAVVPFGVGSSLEG
HVIPYEKGITVDFSLMNKILEIREKDFLVKVQPGVTRSQLNKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVM
RDQVRDLEVVLANGEVIHTGNLAAKSSSGYHLNGIFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPAINDAVEAVINI
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDHKCKEVAFETETAARNKLWDA
RHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQQAKETLEKNGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADEINE
SIVLYALKRGGTCTGEHGVGIGKRKYQEEEHGAALFVMEKIKKALDPQNILNPNKVFQLRDRS
>Mature_463_residues
MEITVERLVNELKGVLPEDRVVINTTVRELHSKDESYHASSLPDVVVFPRTTEEVSTIMKIASEHGTAVVPFGVGSSLEG
HVIPYEKGITVDFSLMNKILEIREKDFLVKVQPGVTRSQLNKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVM
RDQVRDLEVVLANGEVIHTGNLAAKSSSGYHLNGIFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPAINDAVEAVINI
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDHKCKEVAFETETAARNKLWDA
RHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQQAKETLEKNGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADEINE
SIVLYALKRGGTCTGEHGVGIGKRKYQEEEHGAALFVMEKIKKALDPQNILNPNKVFQLRDRS

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=454, Percent_Identity=47.3568281938326, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI37595754, Length=477, Percent_Identity=44.8637316561845, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI119964728, Length=433, Percent_Identity=27.0207852193995, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI4501993, Length=219, Percent_Identity=30.1369863013699, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1789351, Length=465, Percent_Identity=29.247311827957, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1787977, Length=508, Percent_Identity=27.1653543307087, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI48994907, Length=491, Percent_Identity=24.6435845213849, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71992373, Length=462, Percent_Identity=42.6406926406926, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71992381, Length=462, Percent_Identity=41.7748917748918, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71992392, Length=361, Percent_Identity=46.814404432133, Blast_Score=334, Evalue=7e-92,
Organism=Caenorhabditis elegans, GI17534361, Length=453, Percent_Identity=26.4900662251656, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17556096, Length=452, Percent_Identity=25.8849557522124, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320027, Length=474, Percent_Identity=37.5527426160338, Blast_Score=310, Evalue=3e-85,
Organism=Saccharomyces cerevisiae, GI6320764, Length=470, Percent_Identity=25.7446808510638, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320023, Length=473, Percent_Identity=25.5813953488372, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI18921117, Length=431, Percent_Identity=26.4501160092807, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24639277, Length=431, Percent_Identity=26.4501160092807, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24639275, Length=431, Percent_Identity=26.4501160092807, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24653753, Length=219, Percent_Identity=31.0502283105023, Blast_Score=105, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.2.4

Molecular weight: Translated: 50850; Mature: 50850

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITVERLVNELKGVLPEDRVVINTTVRELHSKDESYHASSLPDVVVFPRTTEEVSTIMK
CCEEHHHHHHHHHCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHH
IASEHGTAVVPFGVGSSLEGHVIPYEKGITVDFSLMNKILEIREKDFLVKVQPGVTRSQL
HHHCCCCEEEEECCCCCCCCCEEECCCCCEEEHHHHHHHHHHHCCCEEEEECCCCCHHHH
NKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVMRDQVRDLEVVLANGEVIHTG
HHHHHHHCCEEEECCCCCCCCCCEEECCCCCCHHEEHHHHHHHHCCEEEEEECCCEEECC
NLAAKSSSGYHLNGIFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPAINDAVEAVINI
CCEECCCCCEEEEEEEECCCCCEEEEEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHH
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDH
HHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
KCKEVAFETETAARNKLWDARHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQQAKETL
HHHHHHHCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHH
EKNGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADEINESIVLYALKRGGTCTGEHGVG
HHCCCEEEEHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCC
IGKRKYQEEEHGAALFVMEKIKKALDPQNILNPNKVFQLRDRS
CCHHHCCHHHCCCCHHHHHHHHHHCCHHHCCCCCCEEEEECCC
>Mature Secondary Structure
MEITVERLVNELKGVLPEDRVVINTTVRELHSKDESYHASSLPDVVVFPRTTEEVSTIMK
CCEEHHHHHHHHHCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHH
IASEHGTAVVPFGVGSSLEGHVIPYEKGITVDFSLMNKILEIREKDFLVKVQPGVTRSQL
HHHCCCCEEEEECCCCCCCCCEEECCCCCEEEHHHHHHHHHHHCCCEEEEECCCCCHHHH
NKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVMRDQVRDLEVVLANGEVIHTG
HHHHHHHCCEEEECCCCCCCCCCEEECCCCCCHHEEHHHHHHHHCCEEEEEECCCEEECC
NLAAKSSSGYHLNGIFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPAINDAVEAVINI
CCEECCCCCEEEEEEEECCCCCEEEEEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHH
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDH
HHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
KCKEVAFETETAARNKLWDARHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQQAKETL
HHHHHHHCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHH
EKNGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADEINESIVLYALKRGGTCTGEHGVG
HHCCCEEEEHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCC
IGKRKYQEEEHGAALFVMEKIKKALDPQNILNPNKVFQLRDRS
CCHHHCCHHHCCCCHHHHHHHHHHCCHHHCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; ferricytochrome c

Specific reaction: (R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]