BacMap

Definition	Bacillus cereus E33L, complete genome.
Accession	NC_006274
Length	5,300,915

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The map label for this gene is yngF [H]

Identifier: 52142006

GI number: 52142006

Start: 3358744

End: 3359535

Strand: Reverse

Name: yngF [H]

Synonym: BCZK3237

Alternate gene names: 52142006

Gene position: 3359535-3358744 (Counterclockwise)

Preceding gene: 52142005

Following gene: 52142007

Centisome position: 63.38

GC content: 36.36

Gene sequence:

>792_bases
ATGAAAAATGAACGATTAGTAATATGCAGTAAAAAAGGGAGTAGTGCAGTCATTACAATTCAAAACCCACCAGTCAACAC
TTTAAGTTTAGAAGTAGTGCAACAATTAATAAATGTATTAGAGGAAATAGAGATGGACGATGATGTTGCAGTCGTAATTA
TAACTGGAATTGGGGAGAAAGCCTTTGTAGCTGGAGGGGATATAAAAGAATTTCCAGGTTGGATAGGAAAAGGTGAAAAA
TATGCTGAGATGAAATCAATAGAATTACAAAAGCCTCTTAATCAACTAGAGAATTTGTCTAAACCAACTATAGCTGCTAT
TAATGGGTTAGCACTAGGCGGAGGTTGTGAATTAGCACTTGCTTGTGATTTAAGAGTAATAGAAGAACAAGCTTTAATTG
GATTACCAGAAATTACGTTAGGTCTATTTCCTGGGGCTGGAGGAACTCAGCGCTTACCTAGATTAATTGGAGAAGGAAAA
GCAAAAGAGATGATGTTCACAGGAAAACCAATTACAGCGAAAGAAGCAAAAGAAATAAATTTAGTTAACTACATTACCTC
TCGAGGAGAGGCGTTAAATAAAGCAAAAGAGATTGCTAAAGATATAAGTGAATTTTCTCTTCCAGCACTTTCCTATATGA
AGTTAGCAATTCGTGAAGGTTTGGCAGTACCTTTACAAGAAGGGCTTCAGATCGAAGCGCGTTATTTTGGGAAAGTGTTT
CAAACAGAAGATGTGAAGGAGGGGGTAAAGGCATTTATTGAAAAAAGAGTACCGCGTTTCACCAATAAATAA

Upstream 100 bases:

>100_bases
TTTCTAAAAAGGGAAATATATTCACTAATTCAGGCTAATAGTGAATAAATCGAAGAAACTTGAACTTGAGAAAAATTAGG
GATTTAAAAGGGGACTTTTT

Downstream 100 bases:

>100_bases
AAAGTATTGGGGTGTATTTTACAATTGCCCACACAGATGCTACTTTTAAAGCTATTTATTTGATTCTTAAAATATCTTTC
AGAGCTGTTTCATCTAAGAA

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKNERLVICSKKGSSAVITIQNPPVNTLSLEVVQQLINVLEEIEMDDDVAVVIITGIGEKAFVAGGDIKEFPGWIGKGEK
YAEMKSIELQKPLNQLENLSKPTIAAINGLALGGGCELALACDLRVIEEQALIGLPEITLGLFPGAGGTQRLPRLIGEGK
AKEMMFTGKPITAKEAKEINLVNYITSRGEALNKAKEIAKDISEFSLPALSYMKLAIREGLAVPLQEGLQIEARYFGKVF
QTEDVKEGVKAFIEKRVPRFTNK

Sequences:

>Translated_263_residues
MKNERLVICSKKGSSAVITIQNPPVNTLSLEVVQQLINVLEEIEMDDDVAVVIITGIGEKAFVAGGDIKEFPGWIGKGEK
YAEMKSIELQKPLNQLENLSKPTIAAINGLALGGGCELALACDLRVIEEQALIGLPEITLGLFPGAGGTQRLPRLIGEGK
AKEMMFTGKPITAKEAKEINLVNYITSRGEALNKAKEIAKDISEFSLPALSYMKLAIREGLAVPLQEGLQIEARYFGKVF
QTEDVKEGVKAFIEKRVPRFTNK
>Mature_263_residues
MKNERLVICSKKGSSAVITIQNPPVNTLSLEVVQQLINVLEEIEMDDDVAVVIITGIGEKAFVAGGDIKEFPGWIGKGEK
YAEMKSIELQKPLNQLENLSKPTIAAINGLALGGGCELALACDLRVIEEQALIGLPEITLGLFPGAGGTQRLPRLIGEGK
AKEMMFTGKPITAKEAKEINLVNYITSRGEALNKAKEIAKDISEFSLPALSYMKLAIREGLAVPLQEGLQIEARYFGKVF
QTEDVKEGVKAFIEKRVPRFTNK

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=265, Percent_Identity=36.2264150943396, Blast_Score=160, Evalue=9e-40,
Organism=Homo sapiens, GI4502327, Length=246, Percent_Identity=39.4308943089431, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=38.7640449438202, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI68989263, Length=189, Percent_Identity=34.3915343915344, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=30, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI150378541, Length=242, Percent_Identity=30.9917355371901, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI37594469, Length=153, Percent_Identity=37.2549019607843, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI37594471, Length=153, Percent_Identity=37.2549019607843, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI70995211, Length=241, Percent_Identity=29.4605809128631, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI4757968, Length=245, Percent_Identity=22.8571428571429, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI49355787, Length=245, Percent_Identity=22.8571428571429, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI25453481, Length=245, Percent_Identity=22.8571428571429, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI51538573, Length=245, Percent_Identity=22.8571428571429, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI4757966, Length=227, Percent_Identity=23.3480176211454, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI51538581, Length=227, Percent_Identity=23.3480176211454, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI261878539, Length=95, Percent_Identity=41.0526315789474, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI62530384, Length=256, Percent_Identity=24.609375, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI221136756, Length=236, Percent_Identity=23.728813559322, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI221136753, Length=236, Percent_Identity=23.728813559322, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI221307494, Length=236, Percent_Identity=23.728813559322, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI157694516, Length=229, Percent_Identity=25.764192139738, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI213417737, Length=229, Percent_Identity=25.764192139738, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI116235487, Length=188, Percent_Identity=26.063829787234, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI157694520, Length=155, Percent_Identity=27.741935483871, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI45643119, Length=205, Percent_Identity=26.3414634146341, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI260275230, Length=205, Percent_Identity=26.3414634146341, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI260274832, Length=205, Percent_Identity=26.3414634146341, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI295842266, Length=128, Percent_Identity=32.03125, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787659, Length=266, Percent_Identity=38.3458646616541, Blast_Score=168, Evalue=3e-43,
Organism=Escherichia coli, GI221142681, Length=258, Percent_Identity=33.3333333333333, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1788682, Length=221, Percent_Identity=39.3665158371041, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1788597, Length=251, Percent_Identity=30.6772908366534, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1790281, Length=176, Percent_Identity=35.7954545454545, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI87082183, Length=243, Percent_Identity=27.1604938271605, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787660, Length=249, Percent_Identity=29.718875502008, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=38.1889763779528, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI25145438, Length=261, Percent_Identity=37.1647509578544, Blast_Score=161, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=34.5454545454545, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17558304, Length=182, Percent_Identity=37.9120879120879, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI25144157, Length=209, Percent_Identity=31.1004784688995, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25144276, Length=165, Percent_Identity=36.3636363636364, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25144160, Length=209, Percent_Identity=31.1004784688995, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17508953, Length=165, Percent_Identity=36.3636363636364, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17508951, Length=165, Percent_Identity=36.3636363636364, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=232, Percent_Identity=31.4655172413793, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=253, Percent_Identity=29.2490118577075, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=255, Percent_Identity=25.0980392156863, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=251, Percent_Identity=26.6932270916335, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=200, Percent_Identity=27.5, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=186, Percent_Identity=27.9569892473118, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=198, Percent_Identity=26.7676767676768, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=39.5161290322581, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=39.5161290322581, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24653139, Length=250, Percent_Identity=38.8, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24650670, Length=232, Percent_Identity=31.0344827586207, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI21357171, Length=264, Percent_Identity=30.6818181818182, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI19922422, Length=247, Percent_Identity=27.1255060728745, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24654903, Length=251, Percent_Identity=23.5059760956175, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19921000, Length=173, Percent_Identity=36.9942196531792, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24583077, Length=173, Percent_Identity=36.9942196531792, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24583079, Length=173, Percent_Identity=36.9942196531792, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI28571730, Length=154, Percent_Identity=32.4675324675325, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI28571729, Length=154, Percent_Identity=32.4675324675325, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921018, Length=208, Percent_Identity=32.2115384615385, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19920382, Length=244, Percent_Identity=26.6393442622951, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583165, Length=240, Percent_Identity=27.9166666666667, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI45550169, Length=185, Percent_Identity=30.2702702702703, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 28610; Mature: 28610

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNERLVICSKKGSSAVITIQNPPVNTLSLEVVQQLINVLEEIEMDDDVAVVIITGIGEK
CCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCE
AFVAGGDIKEFPGWIGKGEKYAEMKSIELQKPLNQLENLSKPTIAAINGLALGGGCELAL
EEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCEEECCCCCEEE
ACDLRVIEEQALIGLPEITLGLFPGAGGTQRLPRLIGEGKAKEMMFTGKPITAKEAKEIN
EECHHHHHHHHHCCCCCHHEEECCCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHH
LVNYITSRGEALNKAKEIAKDISEFSLPALSYMKLAIREGLAVPLQEGLQIEARYFGKVF
HHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHCCCEEEHHHHHHHH
QTEDVKEGVKAFIEKRVPRFTNK
CCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKNERLVICSKKGSSAVITIQNPPVNTLSLEVVQQLINVLEEIEMDDDVAVVIITGIGEK
CCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCE
AFVAGGDIKEFPGWIGKGEKYAEMKSIELQKPLNQLENLSKPTIAAINGLALGGGCELAL
EEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCEEECCCCCEEE
ACDLRVIEEQALIGLPEITLGLFPGAGGTQRLPRLIGEGKAKEMMFTGKPITAKEAKEIN
EECHHHHHHHHHCCCCCHHEEECCCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHH
LVNYITSRGEALNKAKEIAKDISEFSLPALSYMKLAIREGLAVPLQEGLQIEARYFGKVF
HHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHCCCEEEHHHHHHHH
QTEDVKEGVKAFIEKRVPRFTNK
CCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]