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The map label for this gene is ykuN [H]

Identifier: 52141993

GI number: 52141993

Start: 3372688

End: 3373152

Strand: Reverse

Name: ykuN [H]

Synonym: BCZK3250

Alternate gene names: 52141993

Gene position: 3373152-3372688 (Counterclockwise)

Preceding gene: 52141992

Following gene: 52141995

Centisome position: 63.63

GC content: 37.42

Gene sequence:

>465_bases
TTGTTAGAGGGGGATGCCGAAGTGGCGAAAATTTTAATAGCTTATGCAAGTATGTCAGGAAATACAGAGAGTATTGCTGA
TTTAATTAAAGTTAGTTTAGATGCTTTTGATCATGAAGTAGTATTGCAAGAAATGGAAGGTATGGATGCTGAAGAATTAT
TAGTTTATGATGGAATCATTTTAGGATCTTATACGTGGGGCGATGGTGAATTGCCATTTGAAGCGGAAGATTTCCATGAG
GACTTAGAAAATATAGATTTATCAGGGAAGAAAGTAGCGGTATTTGGATCAGGTGATACGGCGTATGAACTGTTTTGTGA
AGCGGTAACGATATTTGAAGAAAGGCTTGTAGAACGCGGTGCAGAGCTTGTACAAGAAGGATTGAAAATCGAACTAGCTC
CAGAAGATGAAGAGGATGTTGAAAAATGCAGTAATTTTGCAATTGCATTTGCTGAGAAGTTCTAA

Upstream 100 bases:

>100_bases
TGTATAGTCATCTGTGCTTTTTTGTATGGAATAAATAAAAATGAAAAATATAATTGAAATTGATTATCATTATTAATATA
ATGAGAATGAGAATCATTTA

Downstream 100 bases:

>100_bases
GAATGGGAGTGTCAACGTGGAGACAATGTTAAGTGCGACCGCTATTACAAAGTTAAGAGATGGGAAGCTTATGTTAGCTA
CTACATATAATGGTTTATTT

Product: flavodoxin

Products: oxidized flavodoxin; NH3; ADP; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MLEGDAEVAKILIAYASMSGNTESIADLIKVSLDAFDHEVVLQEMEGMDAEELLVYDGIILGSYTWGDGELPFEAEDFHE
DLENIDLSGKKVAVFGSGDTAYELFCEAVTIFEERLVERGAELVQEGLKIELAPEDEEDVEKCSNFAIAFAEKF

Sequences:

>Translated_154_residues
MLEGDAEVAKILIAYASMSGNTESIADLIKVSLDAFDHEVVLQEMEGMDAEELLVYDGIILGSYTWGDGELPFEAEDFHE
DLENIDLSGKKVAVFGSGDTAYELFCEAVTIFEERLVERGAELVQEGLKIELAPEDEEDVEKCSNFAIAFAEKF
>Mature_154_residues
MLEGDAEVAKILIAYASMSGNTESIADLIKVSLDAFDHEVVLQEMEGMDAEELLVYDGIILGSYTWGDGELPFEAEDFHE
DLENIDLSGKKVAVFGSGDTAYELFCEAVTIFEERLVERGAELVQEGLKIELAPEDEEDVEKCSNFAIAFAEKF

Specific function: Low-potential electron donor to a number of redox enzymes (Potential) [H]

COG id: COG0716

COG function: function code C; Flavodoxins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Escherichia coli, GI1786900, Length=116, Percent_Identity=37.9310344827586, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: 242 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010087
- InterPro:   IPR001094
- InterPro:   IPR008254 [H]

Pfam domain/function: PF00258 Flavodoxin_1 [H]

EC number: 1.19.6.1

Molecular weight: Translated: 16998; Mature: 16998

Theoretical pI: Translated: 3.65; Mature: 3.65

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEGDAEVAKILIAYASMSGNTESIADLIKVSLDAFDHEVVLQEMEGMDAEELLVYDGII
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCEE
LGSYTWGDGELPFEAEDFHEDLENIDLSGKKVAVFGSGDTAYELFCEAVTIFEERLVERG
EECEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
AELVQEGLKIELAPEDEEDVEKCSNFAIAFAEKF
HHHHHCCCEEEECCCCHHHHHHHHHHHHHEEECC
>Mature Secondary Structure
MLEGDAEVAKILIAYASMSGNTESIADLIKVSLDAFDHEVVLQEMEGMDAEELLVYDGII
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCEE
LGSYTWGDGELPFEAEDFHEDLENIDLSGKKVAVFGSGDTAYELFCEAVTIFEERLVERG
EECEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
AELVQEGLKIELAPEDEEDVEKCSNFAIAFAEKF
HHHHHCCCEEEECCCCHHHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced flavodoxin; H+; N2; ATP

Specific reaction: 6 reduced flavodoxin + 6 H+ + N2 + n ATP = 6 oxidized flavodoxin + 2 NH3 + n ADP + n phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]