Click here to switch to the map view.

The map label for this gene is rihA [H]

Identifier: 52141983

GI number: 52141983

Start: 3387537

End: 3388841

Strand: Reverse

Name: rihA [H]

Synonym: BCZK3260

Alternate gene names: 52141983

Gene position: 3388841-3387537 (Counterclockwise)

Preceding gene: 52141982

Following gene: 52141984

Centisome position: 63.93

GC content: 36.4

Gene sequence:

>1305_bases
ATGGTTAAATTTATTTTCTTTCGGTGGATTAGAAGCAGCATTCATTTGGCCGATTGTATTAGGGTTATATTGGAGGAAAG
GAAATGCAACAGGAGCGCTTGCCTCCATTTTAGTTGGAGTAAGCTCATATATGTGCATTCATCTTTTCTATCCAAATCCG
TTCGGTATACATACAGTTGTCTTCCCGATTTTTTTTGCGTTTATCGCTTATATCTTAGGAAGCATGGTTGCTGTGAAGAA
AAACAGTATAGGTTAGATGAAAAACGTAGTCCTTACAGGACTGCGTTTTTTCGTATAAATTTGTTCTTTTTATTCTGTAA
TCCTGTGAATATTCATAAATTAATAACAATTGAGGGGATGGAGTTGAGGGCGATGAAAAAAGTATTATTTTTAGGAGACC
CAGGAATTGATGACTCTTTAGCAATTATGTATGGATTGTTGCATCCTGATATTGATATTGTTGGTGTAGTAACTGGATAT
GGAAATGTAACGCAAGAAAAGGCGACAAGTAATGCGGCATATTTATTGCAACTGGCAGGACGGGAAGATATACCTATTAT
TAATGGTGCGAAAATCCCTTTATCTGGAGATATTACAACGTATTATCCAGAAATTCATGGGGCGGAAGGCTTAGGACCAA
TTCGACCGCCGAAAAATCTTTCTCCAAATATAAGGCCTTTTTGTGAGTTTTTTGACATTCTTGAAAAATATAAAGGAGAA
TTAATTATAGTTGATGCTGGGAGGTCAACGACACTTGCAACAGCATTTATTTTAGAAAAACCATTGATGAAGTATGTGAA
AGAATATTATATAATGGGCGGTGCTTTTTTAATGCCTGGAAATGTTACACCAGTCGCAGAAGCGAATTTTCATGGTGACC
CTATTGCATCACAATTAGTCATGCAAAATGCCAAGAATGTGACGTTGGTGCCGCTGAATGTTACATCTGAAGCTATAATC
ACGCCAGAGATGGTAAAGTACATTACGAAACATTCTAAAACGAGTTTCAATAAATTAATTGAACCGATTTTTACGTATTA
TTATAAAGCTTATAGAAAGTTAAATCCGAAAATAACAGGAAGTCCGGTACATGACGTTGTTACAATGATGGTCGCGGCGA
ATCCTTCAATACTGGATTATGTGTATCGTCGTGTAGATGTAGATACAGTGGGGATTGCAAAAGGAGAAAGTATTGCAGAT
TTCCGTCCTCAACCTGATGCAAAAGCCTTAAAAAATTGGGTACGAATTGGTTGGTCATTACATTATAAAAAATTCCTTGA
GGATTTTGTGAAAATCATGACGTAG

Upstream 100 bases:

>100_bases
ATGCGGCAGACAGTACGATTAGAAAAGGAAGTTTATGGATTACTGCTATCGTAGGATTGCTTGTTTATGCAGCAGCAATT
AAACCACCAGATTTTTTAAT

Downstream 100 bases:

>100_bases
ACATAATGGGAGTATACAAGTTAAAATAATGGAGATGGTTATTCAAATAACCATCTCCATTATTTTGTTTTTTTAAGATA
GAGAGAAAGAGTTACACAAT

Product: inosine-uridine preferring nucleoside hydrolase

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MVKFIFFRWIRSSIHLADCIRVILEERKCNRSACLHFSWSKLIYVHSSFLSKSVRYTYSCLPDFFCVYRLYLRKHGCCEE
KQYRLDEKRSPYRTAFFRINLFFLFCNPVNIHKLITIEGMELRAMKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGY
GNVTQEKATSNAAYLLQLAGREDIPIINGAKIPLSGDITTYYPEIHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKGE
LIIVDAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMPGNVTPVAEANFHGDPIASQLVMQNAKNVTLVPLNVTSEAII
TPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKITGSPVHDVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIAD
FRPQPDAKALKNWVRIGWSLHYKKFLEDFVKIMT

Sequences:

>Translated_434_residues
MVKFIFFRWIRSSIHLADCIRVILEERKCNRSACLHFSWSKLIYVHSSFLSKSVRYTYSCLPDFFCVYRLYLRKHGCCEE
KQYRLDEKRSPYRTAFFRINLFFLFCNPVNIHKLITIEGMELRAMKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGY
GNVTQEKATSNAAYLLQLAGREDIPIINGAKIPLSGDITTYYPEIHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKGE
LIIVDAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMPGNVTPVAEANFHGDPIASQLVMQNAKNVTLVPLNVTSEAII
TPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKITGSPVHDVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIAD
FRPQPDAKALKNWVRIGWSLHYKKFLEDFVKIMT
>Mature_434_residues
MVKFIFFRWIRSSIHLADCIRVILEERKCNRSACLHFSWSKLIYVHSSFLSKSVRYTYSCLPDFFCVYRLYLRKHGCCEE
KQYRLDEKRSPYRTAFFRINLFFLFCNPVNIHKLITIEGMELRAMKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGY
GNVTQEKATSNAAYLLQLAGREDIPIINGAKIPLSGDITTYYPEIHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKGE
LIIVDAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMPGNVTPVAEANFHGDPIASQLVMQNAKNVTLVPLNVTSEAII
TPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKITGSPVHDVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIAD
FRPQPDAKALKNWVRIGWSLHYKKFLEDFVKIMT

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=280, Percent_Identity=29.2857142857143, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788486, Length=279, Percent_Identity=27.2401433691756, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1786213, Length=306, Percent_Identity=28.4313725490196, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32565474, Length=200, Percent_Identity=29, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17565698, Length=200, Percent_Identity=29, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320608, Length=275, Percent_Identity=30.5454545454545, Blast_Score=112, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 49557; Mature: 49557

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKFIFFRWIRSSIHLADCIRVILEERKCNRSACLHFSWSKLIYVHSSFLSKSVRYTYSC
CCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHH
LPDFFCVYRLYLRKHGCCEEKQYRLDEKRSPYRTAFFRINLFFLFCNPVNIHKLITIEGM
HHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHEEEEEEEEEEEEECCCCEEEEEEECCC
ELRAMKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQLAG
HHHHHHHHHEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCCEEEEEECC
REDIPIINGAKIPLSGDITTYYPEIHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKGE
CCCCCEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
LIIVDAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMPGNVTPVAEANFHGDPIASQLV
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHH
MQNAKNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKITG
HHCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
SPVHDVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSL
CCHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HYKKFLEDFVKIMT
HHHHHHHHHHHHCC
>Mature Secondary Structure
MVKFIFFRWIRSSIHLADCIRVILEERKCNRSACLHFSWSKLIYVHSSFLSKSVRYTYSC
CCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHH
LPDFFCVYRLYLRKHGCCEEKQYRLDEKRSPYRTAFFRINLFFLFCNPVNIHKLITIEGM
HHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHEEEEEEEEEEEEECCCCEEEEEEECCC
ELRAMKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQLAG
HHHHHHHHHEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCCEEEEEECC
REDIPIINGAKIPLSGDITTYYPEIHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKGE
CCCCCEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
LIIVDAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMPGNVTPVAEANFHGDPIASQLV
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHH
MQNAKNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKITG
HHCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
SPVHDVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSL
CCHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HYKKFLEDFVKIMT
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA