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The map label for this gene is sigW [H]

Identifier: 52141944

GI number: 52141944

Start: 3431017

End: 3431550

Strand: Reverse

Name: sigW [H]

Synonym: BCZK3297

Alternate gene names: 52141944

Gene position: 3431550-3431017 (Counterclockwise)

Preceding gene: 52141945

Following gene: 52141947

Centisome position: 64.74

GC content: 32.02

Gene sequence:

>534_bases
GTGAAAGTAATCCCTTTATATAAAATGTTGGTAAAAGAAGAAACAGATGTTTCCTTAGCGAAAAAAGGTGATCATGAAGC
TTTTATGACTCTTATACATACAGAAAAAGTGAAGATGTATCGTATTGCAAAGGCTATGTTACGTGATGAGACAAATATAG
AGGATGCGATACAAACAACAATTTTAAAAGCCTATGAAAATATAAAAAAAATAAAGAAGGAAGAGTTTTTTCAAACTTGG
TTAATTCGAATTTTAATGAATGAATGTAACAACATTATTAGAACGTATAAAAAGGTAATTGTGACAGAAGAGAATGATTA
CAATATGAGTGCGTGCGATCAGTATGAAGATATAGATTTGTGTAATGCGATTCAATCGTTACATGAAGAGTTAAGAGCTG
TTACGGTGCTTTATTATTATGAAGATATGAATCAAGAGAGTATAGCGAAGCTTTTAGAAGTACCAAAAGGAACAGTGAAA
TCGAGATTGGCACGTGCGAGGGAACAACTACAGAAACGATTAAAGATGGAATAG

Upstream 100 bases:

>100_bases
TTTTATGAAAATATTCAAAAAAATGAACCTTTTTCTAGGCTCATACGTCTAATACATGTAAAGTTAAATTTGAAGCGAGG
ATGAAACAGGAGGAGCAATT

Downstream 100 bases:

>100_bases
GGGGTGCAAAGGATGGATAATGAAAAAAAATTTGATGAGAAATTAAAAAAGAGAATAAACGAAGAGAATGTTATAGTACC
GCTAGAGTTAAATGAAAAGA

Product: RNA polymerase ECF-type sigma factor

Products: diphosphate; RNAn+1

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MKVIPLYKMLVKEETDVSLAKKGDHEAFMTLIHTEKVKMYRIAKAMLRDETNIEDAIQTTILKAYENIKKIKKEEFFQTW
LIRILMNECNNIIRTYKKVIVTEENDYNMSACDQYEDIDLCNAIQSLHEELRAVTVLYYYEDMNQESIAKLLEVPKGTVK
SRLARAREQLQKRLKME

Sequences:

>Translated_177_residues
MKVIPLYKMLVKEETDVSLAKKGDHEAFMTLIHTEKVKMYRIAKAMLRDETNIEDAIQTTILKAYENIKKIKKEEFFQTW
LIRILMNECNNIIRTYKKVIVTEENDYNMSACDQYEDIDLCNAIQSLHEELRAVTVLYYYEDMNQESIAKLLEVPKGTVK
SRLARAREQLQKRLKME
>Mature_177_residues
MKVIPLYKMLVKEETDVSLAKKGDHEAFMTLIHTEKVKMYRIAKAMLRDETNIEDAIQTTILKAYENIKKIKKEEFFQTW
LIRILMNECNNIIRTYKKVIVTEENDYNMSACDQYEDIDLCNAIQSLHEELRAVTVLYYYEDMNQESIAKLLEVPKGTVK
SRLARAREQLQKRLKME

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=173, Percent_Identity=24.8554913294798, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: 2.7.7.6

Molecular weight: Translated: 20962; Mature: 20962

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIPLYKMLVKEETDVSLAKKGDHEAFMTLIHTEKVKMYRIAKAMLRDETNIEDAIQTT
CCCHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ILKAYENIKKIKKEEFFQTWLIRILMNECNNIIRTYKKVIVTEENDYNMSACDQYEDIDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHH
CNAIQSLHEELRAVTVLYYYEDMNQESIAKLLEVPKGTVKSRLARAREQLQKRLKME
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVIPLYKMLVKEETDVSLAKKGDHEAFMTLIHTEKVKMYRIAKAMLRDETNIEDAIQTT
CCCHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ILKAYENIKKIKKEEFFQTWLIRILMNECNNIIRTYKKVIVTEENDYNMSACDQYEDIDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHH
CNAIQSLHEELRAVTVLYYYEDMNQESIAKLLEVPKGTVKSRLARAREQLQKRLKME
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nucleoside triphosphate; RNAn

Specific reaction: nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]