| Definition | Bacillus cereus E33L, complete genome. |
|---|---|
| Accession | NC_006274 |
| Length | 5,300,915 |
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The map label for this gene is dnaE [H]
Identifier: 52141929
GI number: 52141929
Start: 3449059
End: 3450006
Strand: Reverse
Name: dnaE [H]
Synonym: BCZK3316
Alternate gene names: 52141929
Gene position: 3450006-3449059 (Counterclockwise)
Preceding gene: 52141927
Following gene: 52141928
Centisome position: 65.08
GC content: 33.02
Gene sequence:
>948_bases GTGGGGAATATATCCTTACCGTTAAATTATGTTGTAATTGATTTTGAAACAACAGGATTTAACCCTTATAATGATAAAAT CATTCAAGTGGCAGCAGTGAAATATCGTAATCATGAACTAGTAGATCAATTTGTTTCCTATGTGAATCCAGAGCGTCCAA TTCCGGACCGAATAACGAGTTTAACGGGTATTACAAATTATCGTGTTTCAGATGCACCGACAATTGAAGAAGTATTACCT GTATTTTTAGCATTTTTACATACAAATGTAATTGTCGCTCATAATGCATCTTTTGATATGCGCTTTTTGAAAAGTAATGT GAATATGCTGGGGTTACCGGAACCGAAAAATAAAGTGATTGATACTGTATTTTTAGCGAAGAAATATATGAAGCACGCGC CTAATCATAAACTTGAAACGTTAAAGAGAATGCTCGGAATTCGTTTAAGTTCTCATAATGCATTTGATGATTGTATTACG TGTGCAGCTGTTTATCAAAAATGTGCATCTATTGAAGAAGAGGCAAAAAGGAAATTGAATAAGGAAGCACTTGATGAAAC AGTAGTATATGAAGCGGTAAAAGAGATACTTGTACGAAATAAACGTGATATCGAATGGATTCGTTGCATGAATGTAGGGA GCTATTTAGATATAAAAGCTTTTTATCCAGTTATGAGATTAAAGGTAAAAGGGCGCAAGAAATACGTATTAACAGACATA TTAGAAGATGATGTGAAAGAAATATGTACGAGTTTAGCATGTGAACCGGCATTAAAAAGTGAAGTTGGTAATACGAGAAT TATGCTGAATAGCTTAGAGGATGTCTTGAAATTAGAAAGTTATATTTTAGAACAGTATGATTTCGTACTGCAAGCGCTGC GTGATTATAAACAAAGTGAAATGAATGCAGATGAAAAGTTAAAAGAGTATTTAAATATTATGGTATAA
Upstream 100 bases:
>100_bases TGGAATATAATCATTTCTGCCTTTTCTTTTTCACGTTGTGAAAGCTTCATGATATGATAAAAGAAATATAAACGTTGAGA GATAAAGGAGTGTATTTGGA
Downstream 100 bases:
>100_bases AAAACTCCGTGTACGATTTATTGTACACGGAGTTTTTTTAGGAGTACTAAAACATAAAATTTTCTTACAAATCTGATTAA AGAGTTTTTCCTTTGCTCAT
Product: DNA polymerase III subunit epsilon
Products: NA
Alternate protein names: PolIII [H]
Number of amino acids: Translated: 315; Mature: 314
Protein sequence:
>315_residues MGNISLPLNYVVIDFETTGFNPYNDKIIQVAAVKYRNHELVDQFVSYVNPERPIPDRITSLTGITNYRVSDAPTIEEVLP VFLAFLHTNVIVAHNASFDMRFLKSNVNMLGLPEPKNKVIDTVFLAKKYMKHAPNHKLETLKRMLGIRLSSHNAFDDCIT CAAVYQKCASIEEEAKRKLNKEALDETVVYEAVKEILVRNKRDIEWIRCMNVGSYLDIKAFYPVMRLKVKGRKKYVLTDI LEDDVKEICTSLACEPALKSEVGNTRIMLNSLEDVLKLESYILEQYDFVLQALRDYKQSEMNADEKLKEYLNIMV
Sequences:
>Translated_315_residues MGNISLPLNYVVIDFETTGFNPYNDKIIQVAAVKYRNHELVDQFVSYVNPERPIPDRITSLTGITNYRVSDAPTIEEVLP VFLAFLHTNVIVAHNASFDMRFLKSNVNMLGLPEPKNKVIDTVFLAKKYMKHAPNHKLETLKRMLGIRLSSHNAFDDCIT CAAVYQKCASIEEEAKRKLNKEALDETVVYEAVKEILVRNKRDIEWIRCMNVGSYLDIKAFYPVMRLKVKGRKKYVLTDI LEDDVKEICTSLACEPALKSEVGNTRIMLNSLEDVLKLESYILEQYDFVLQALRDYKQSEMNADEKLKEYLNIMV >Mature_314_residues GNISLPLNYVVIDFETTGFNPYNDKIIQVAAVKYRNHELVDQFVSYVNPERPIPDRITSLTGITNYRVSDAPTIEEVLPV FLAFLHTNVIVAHNASFDMRFLKSNVNMLGLPEPKNKVIDTVFLAKKYMKHAPNHKLETLKRMLGIRLSSHNAFDDCITC AAVYQKCASIEEEAKRKLNKEALDETVVYEAVKEILVRNKRDIEWIRCMNVGSYLDIKAFYPVMRLKVKGRKKYVLTDIL EDDVKEICTSLACEPALKSEVGNTRIMLNSLEDVLKLESYILEQYDFVLQALRDYKQSEMNADEKLKEYLNIMV
Specific function: Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]
COG id: COG2176
COG function: function code L; DNA polymerase III, alpha subunit (gram-positive type)
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 exonuclease domain [H]
Homologues:
Organism=Escherichia coli, GI1786409, Length=173, Percent_Identity=29.4797687861272, Blast_Score=64, Evalue=1e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR011708 - InterPro: IPR006054 - InterPro: IPR006055 - InterPro: IPR013520 - InterPro: IPR012340 - InterPro: IPR016027 - InterPro: IPR004365 - InterPro: IPR004013 - InterPro: IPR003141 - InterPro: IPR006308 - InterPro: IPR012337 [H]
Pfam domain/function: PF07733 DNA_pol3_alpha; PF00929 Exonuc_X-T; PF02811 PHP; PF01336 tRNA_anti [H]
EC number: =2.7.7.7 [H]
Molecular weight: Translated: 36272; Mature: 36140
Theoretical pI: Translated: 6.78; Mature: 6.78
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.9 %Cys (Translated Protein) 3.2 %Met (Translated Protein) 5.1 %Cys+Met (Translated Protein) 1.9 %Cys (Mature Protein) 2.9 %Met (Mature Protein) 4.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MGNISLPLNYVVIDFETTGFNPYNDKIIQVAAVKYRNHELVDQFVSYVNPERPIPDRITS CCCCCCEEEEEEEEEEECCCCCCCCCEEEEHHHHHCCHHHHHHHHHHCCCCCCCHHHHHH LTGITNYRVSDAPTIEEVLPVFLAFLHTNVIVAHNASFDMRFLKSNVNMLGLPEPKNKVI HHCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCCHHHHHHCCCCEECCCCCHHHHH DTVFLAKKYMKHAPNHKLETLKRMLGIRLSSHNAFDDCITCAAVYQKCASIEEEAKRKLN HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH KEALDETVVYEAVKEILVRNKRDIEWIRCMNVGSYLDIKAFYPVMRLKVKGRKKYVLTDI HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEHHHHHHHHHHEECCCCHHHHHHH LEDDVKEICTSLACEPALKSEVGNTRIMLNSLEDVLKLESYILEQYDFVLQALRDYKQSE HHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH MNADEKLKEYLNIMV CCHHHHHHHHHHHCC >Mature Secondary Structure GNISLPLNYVVIDFETTGFNPYNDKIIQVAAVKYRNHELVDQFVSYVNPERPIPDRITS CCCCCEEEEEEEEEEECCCCCCCCCEEEEHHHHHCCHHHHHHHHHHCCCCCCCHHHHHH LTGITNYRVSDAPTIEEVLPVFLAFLHTNVIVAHNASFDMRFLKSNVNMLGLPEPKNKVI HHCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCCHHHHHHCCCCEECCCCCHHHHH DTVFLAKKYMKHAPNHKLETLKRMLGIRLSSHNAFDDCITCAAVYQKCASIEEEAKRKLN HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH KEALDETVVYEAVKEILVRNKRDIEWIRCMNVGSYLDIKAFYPVMRLKVKGRKKYVLTDI HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEHHHHHHHHHHEECCCCHHHHHHH LEDDVKEICTSLACEPALKSEVGNTRIMLNSLEDVLKLESYILEQYDFVLQALRDYKQSE HHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH MNADEKLKEYLNIMV CCHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 12552129 [H]