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The map label for this gene is alkK [H]

Identifier: 52141913

GI number: 52141913

Start: 3465582

End: 3467195

Strand: Reverse

Name: alkK [H]

Synonym: BCZK3332

Alternate gene names: 52141913

Gene position: 3467195-3465582 (Counterclockwise)

Preceding gene: 52141910

Following gene: 52141912

Centisome position: 65.41

GC content: 38.97

Gene sequence:

>1614_bases
ATGATGATGAATGTACCGCTAACAATTAGTTCTATGATGGAAAGAGCAGAAAAACTGTTTTCGAAGAAAGAAATTGTTTC
ACGGACACATGACACAGTTACGACATTAACGTATAAGCAGTTAGGTGAAAGAACGAGAAGGCTTTCCAGTGCGTTAAAAA
AATTAGGAATTAAAGAAGGCGAGCGTATAGGAACGTTAGCGTGGAATCATCATCGACATGTGGAAGCGTATTTTGCTATT
CCTGGTATTGCTTCCGTTTTGCACACAATTAATATTCGTTTATCTCCTCAACATATTTCATACATTATTCAGCATGCAGA
AGATCGAATTCTACTTATTGATGAAGATCTCGTACCACTCATTGAAAATATTCAATCACAATTATCAACTGTACAAGCTT
ACATTATTATGACTGATAAAGATGAAATTCCAAATACTACGCTAGAACCTGTATATCATTATGAAAAACTATTAGAAGAA
GGCGATCCAAATTTCCAATTCGTAAAAGATATTGATGAAAATACACCTGCTGGTATGTGTTATACGTCAGCGACTACAGG
AAATCCAAAAGGTGTTGTATATACGCATCGTAGTACTGTACTGCATTGTATGGCACTCGGTTTAGCAGATACAGCTGCTT
TATCGGAAAGTGATGCGGCAATGGCTATTGTACCGATGTTCCATGTGAATGCTTGGGGACTACCTTTCGCAGCTACTTGG
TTTGGATCAAAACAAGTGCTTCCAGGACCGATGTTTACGCCGAAAATTTTATTAGAAATGATTCAAGCTGAAAAAGTGAC
GTTAGCTGCTGGTGTGCCGACAATTTGGCTTGGTGTATTACAAGAGTTAGAAAATAATAGTTACGATTTATCTAGTATGA
CGAGAATACTATGCGGTGGTGCAGCTGCACCGAAGAGTGTTATTAAAGCATTTGAACAGAAACATAATGTTCCGTTCATC
CATGCATATGGTATGACCGAAACAAGCCCACTCGTAACACTTGCACGTTTAAAAAGTTATGAAACAGAATTATCGTATGA
AGAGCAATTAGAAATTCGATCAAAACAAGGGTATCTTGTTCCTGGTGTAGAGATGAAAGTAGTCGGTACAAATGGTGAAG
TGAAGTGGGATAGTACGGAGATGGGAGAACTATGTTTACGAGCGCCATGGATCGCTGAAAGCTATTATAACGATGATCGT
ACTGTTGAAGGCTTTCGCGATGGATGGTTATATACAGGAGATGTTGTCACAGTCGATGAGGAAGGTTGCGTAAAAATTGT
CGACCGCACGAAAGATGTGATTAAGAGCGGAGGCGAGTGGATTTCCTCTGTTGACCTTGAAAATGCGTTAATGGCACATG
ATGCTATATTTGAAGCGGCTGTCGTTGCCGTTCCTCATCCGCAGTGGCAAGAGCGACCAGTTGCATGTGTCGTTCAAAAG
AAAAATAGTACAGTAACAAAAGAAGAAATATATGAGTTTTTAAAACCACAGTTTGCAAAGTGGTGGTTACCAGACGATAT
TGTATTTATGGAAGAAATACCGAAAACATCTGTTGGGAAGTTTTTAAAGCAGGCGCTTCGGAAAGAACTCGAGCATTTGC
ATAAAGAAAAATAA

Upstream 100 bases:

>100_bases
AGGAATAATTCAGAAAATACAGAAAAATGTCGTAATTATTTGTAGAAGTCTGTTATAATAAACGAAAAATGAAAACGTTT
TAAAGGGGGATATAACCAAT

Downstream 100 bases:

>100_bases
TTGAATTTATATTTGTAAACTAATAAAGAAAACGAGTTGACAAAAGCTAAATAGAAAGATAATATAACTCCTATGATATG
TTACCTAAAAAACATTTTAA

Product: medium-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEGERIGTLAWNHHRHVEAYFAI
PGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPLIENIQSQLSTVQAYIIMTDKDEIPNTTLEPVYHYEKLLEE
GDPNFQFVKDIDENTPAGMCYTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
FGSKQVLPGPMFTPKILLEMIQAEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGGAAAPKSVIKAFEQKHNVPFI
HAYGMTETSPLVTLARLKSYETELSYEEQLEIRSKQGYLVPGVEMKVVGTNGEVKWDSTEMGELCLRAPWIAESYYNDDR
TVEGFRDGWLYTGDVVTVDEEGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPVACVVQK
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHKEK

Sequences:

>Translated_537_residues
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEGERIGTLAWNHHRHVEAYFAI
PGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPLIENIQSQLSTVQAYIIMTDKDEIPNTTLEPVYHYEKLLEE
GDPNFQFVKDIDENTPAGMCYTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
FGSKQVLPGPMFTPKILLEMIQAEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGGAAAPKSVIKAFEQKHNVPFI
HAYGMTETSPLVTLARLKSYETELSYEEQLEIRSKQGYLVPGVEMKVVGTNGEVKWDSTEMGELCLRAPWIAESYYNDDR
TVEGFRDGWLYTGDVVTVDEEGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPVACVVQK
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHKEK
>Mature_537_residues
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEGERIGTLAWNHHRHVEAYFAI
PGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPLIENIQSQLSTVQAYIIMTDKDEIPNTTLEPVYHYEKLLEE
GDPNFQFVKDIDENTPAGMCYTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
FGSKQVLPGPMFTPKILLEMIQAEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGGAAAPKSVIKAFEQKHNVPFI
HAYGMTETSPLVTLARLKSYETELSYEEQLEIRSKQGYLVPGVEMKVVGTNGEVKWDSTEMGELCLRAPWIAESYYNDDR
TVEGFRDGWLYTGDVVTVDEEGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPVACVVQK
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHKEK

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=556, Percent_Identity=27.8776978417266, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI187761345, Length=548, Percent_Identity=25.7299270072993, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI187761343, Length=548, Percent_Identity=25.7299270072993, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI38505220, Length=509, Percent_Identity=24.950884086444, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI42544132, Length=512, Percent_Identity=23.6328125, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI28416953, Length=539, Percent_Identity=23.5621521335807, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI157311624, Length=514, Percent_Identity=22.568093385214, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI157311622, Length=514, Percent_Identity=22.568093385214, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI58082049, Length=520, Percent_Identity=22.1153846153846, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI115511026, Length=514, Percent_Identity=21.7898832684825, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI122937307, Length=516, Percent_Identity=22.093023255814, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI83745141, Length=512, Percent_Identity=22.8515625, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1788107, Length=521, Percent_Identity=25.5278310940499, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI145693145, Length=363, Percent_Identity=31.9559228650138, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI221142682, Length=513, Percent_Identity=23.1968810916179, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1786810, Length=526, Percent_Identity=23.9543726235741, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1789201, Length=507, Percent_Identity=23.2741617357002, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1790505, Length=539, Percent_Identity=22.6345083487941, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788595, Length=499, Percent_Identity=23.0460921843687, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=518, Percent_Identity=25.0965250965251, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17559526, Length=360, Percent_Identity=31.9444444444444, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17558820, Length=515, Percent_Identity=25.8252427184466, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI71994690, Length=533, Percent_Identity=25.891181988743, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI71994703, Length=533, Percent_Identity=25.891181988743, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI71994694, Length=533, Percent_Identity=25.891181988743, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17560308, Length=536, Percent_Identity=25.5597014925373, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=510, Percent_Identity=24.1176470588235, Blast_Score=139, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17538037, Length=515, Percent_Identity=24.4660194174757, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI32563687, Length=505, Percent_Identity=24.5544554455446, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17560140, Length=514, Percent_Identity=22.568093385214, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=519, Percent_Identity=24.6628131021195, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71985884, Length=372, Percent_Identity=22.8494623655914, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6319699, Length=378, Percent_Identity=27.2486772486773, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6319264, Length=401, Percent_Identity=24.4389027431421, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21355181, Length=500, Percent_Identity=26.8, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24581924, Length=551, Percent_Identity=25.9528130671506, Blast_Score=152, Evalue=6e-37,
Organism=Drosophila melanogaster, GI18859661, Length=359, Percent_Identity=27.2980501392758, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21356441, Length=531, Percent_Identity=23.3521657250471, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24648255, Length=516, Percent_Identity=22.6744186046512, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648253, Length=510, Percent_Identity=21.9607843137255, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24653035, Length=516, Percent_Identity=22.6744186046512, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648260, Length=496, Percent_Identity=21.7741935483871, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI19922652, Length=521, Percent_Identity=22.0729366602687, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24656500, Length=530, Percent_Identity=21.5094339622642, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI161076582, Length=528, Percent_Identity=22.3484848484848, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281365686, Length=519, Percent_Identity=19.6531791907514, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648257, Length=543, Percent_Identity=22.6519337016575, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI20130357, Length=521, Percent_Identity=20.9213051823417, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356947, Length=521, Percent_Identity=20.9213051823417, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21358303, Length=498, Percent_Identity=19.8795180722892, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 60442; Mature: 60442

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEG
CEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ERIGTLAWNHHRHVEAYFAIPGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPL
CEEEEEEECCCCCEEEEEECCHHHHHHHHHEEEECHHHHHHHHHHCCCCEEEECCCHHHH
IENIQSQLSTVQAYIIMTDKDEIPNTTLEPVYHYEKLLEEGDPNFQFVKDIDENTPAGMC
HHHHHHHHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCEE
YTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
EECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEEEEE
FGSKQVLPGPMFTPKILLEMIQAEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGG
CCCCCCCCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHCC
AAAPKSVIKAFEQKHNVPFIHAYGMTETSPLVTLARLKSYETELSYEEQLEIRSKQGYLV
CCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEE
PGVEMKVVGTNGEVKWDSTEMGELCLRAPWIAESYYNDDRTVEGFRDGWLYTGDVVTVDE
CCCEEEEEECCCEEEECCCHHHHHHHCCCHHHHHHCCCCCCCCHHCCCCEEECCEEEECC
EGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPVACVVQK
CCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEC
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHKEK
CCCCCCHHHHHHHHCCHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEG
CEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ERIGTLAWNHHRHVEAYFAIPGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPL
CEEEEEEECCCCCEEEEEECCHHHHHHHHHEEEECHHHHHHHHHHCCCCEEEECCCHHHH
IENIQSQLSTVQAYIIMTDKDEIPNTTLEPVYHYEKLLEEGDPNFQFVKDIDENTPAGMC
HHHHHHHHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCEE
YTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
EECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEEEEE
FGSKQVLPGPMFTPKILLEMIQAEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGG
CCCCCCCCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHCC
AAAPKSVIKAFEQKHNVPFIHAYGMTETSPLVTLARLKSYETELSYEEQLEIRSKQGYLV
CCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEE
PGVEMKVVGTNGEVKWDSTEMGELCLRAPWIAESYYNDDRTVEGFRDGWLYTGDVVTVDE
CCCEEEEEECCCEEEECCCHHHHHHHCCCHHHHHHCCCCCCCCHHCCCCEEECCEEEECC
EGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPVACVVQK
CCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEC
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHKEK
CCCCCCHHHHHHHHCCHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]