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The map label for this gene is phnD [H]

Identifier: 52141855

GI number: 52141855

Start: 3527883

End: 3528935

Strand: Reverse

Name: phnD [H]

Synonym: BCZK3392

Alternate gene names: 52141855

Gene position: 3528935-3527883 (Counterclockwise)

Preceding gene: 52141851

Following gene: 52141854

Centisome position: 66.57

GC content: 32.76

Gene sequence:

>1053_bases
GTGTATAGAATGTGTAAAGATTCTGTAAATAATCAAATTTTTGTAAAAATGTGTGATATATTTCTTCATGTTTATGAAAG
GTATTTTATAACAATGAAAAATGGAGGCACTAATATGTTGAAAAAAGTTTTTGCAATGAGCACAACAGTTGTACTAGCAG
CAGGGTTATTAAGTGGCTGTGGAACAAAGGAATCAAGTGCAAATAAAAATGCAGATACGAAAAAAGGGTATGTACCGAAG
ACTTTAAATGTTCAATTTGTTCCTTCTCAAAATGCAGATACGTTAGAGGCGAAGGCAAAACCACTTGAGAAGCTATTAAG
TGATAAGTTAAATATTCCAGTAAAAGTTAGTATTTCAACGAATTACAATACAATTGTAGAAGCGATAGCATCTAAGCAAG
TAGATGTAGGATTTTTACCCCCAACAGCGTATGTGTTAGCACATGAGAAAAAGGCAGCAAACGTAATTTTACAAGCACAA
CGTTTTGGAGTAGATGATGAAACGGGAGCTCCTACAAAAGATTTAGTAGATTTCTATAAATCTGAGTTTGTTGTTAAGAA
AGATTCAAATATTAATAGTGTAAAAGATTTAAAAGGTAAAAAAATTGGTTATCAAGATGTAACGTCATCAGCGGGATATG
TGTGGCCTGCGGCTGTATTATTAAAAGAGGGCGTTGATCCGTTAAAAGATGTGAAACCTGTTACATTAAAAGGTCACGAT
CAGTCGCTTATTGCTCTTTTAAATGGTGACGTAGATGCAGCTGTTGTATTCCAAGATGCTCGTAATATTGTAAAAAAAGA
TTATCCAAATATATTTGATCAAACGAAAATAGTGAAATTTACGGAGAAAATTCCAAACGATACGATTTCGGTACGTTCGG
ATTTAGATGAAGAGTGGAGCAAGAAATTACAGGATGCTTTCATTGAAATTGGAAAGAATGAGGAAGGTCATAAAATCATT
AAAGAAGTATATTCACATGAAGGATATGTTAAATCTGATGATAGTAAATTTGATATCGTTCGTGAATATGGAAAGAAAGT
AAAAACGCAATAA

Upstream 100 bases:

>100_bases
GTTTAAAGTCACAATTTGATTTGGATTCAATATTATTCACCTTTTCTATTGTTATCTACTAATTAAGCATATCAAATATA
TGAAGGGAATTTTAATGTGA

Downstream 100 bases:

>100_bases
TACATATGGAAATGGCAGCTAGCTGGAAAGGTATGTTTTCAGTTAGTTTGCCGTTTTGAATTCATAGAAGGTAGGTAAGA
TGTGTGATAGAGTTTCGAAA

Product: phosphonate ABC transporter, phosphate-binding protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MYRMCKDSVNNQIFVKMCDIFLHVYERYFITMKNGGTNMLKKVFAMSTTVVLAAGLLSGCGTKESSANKNADTKKGYVPK
TLNVQFVPSQNADTLEAKAKPLEKLLSDKLNIPVKVSISTNYNTIVEAIASKQVDVGFLPPTAYVLAHEKKAANVILQAQ
RFGVDDETGAPTKDLVDFYKSEFVVKKDSNINSVKDLKGKKIGYQDVTSSAGYVWPAAVLLKEGVDPLKDVKPVTLKGHD
QSLIALLNGDVDAAVVFQDARNIVKKDYPNIFDQTKIVKFTEKIPNDTISVRSDLDEEWSKKLQDAFIEIGKNEEGHKII
KEVYSHEGYVKSDDSKFDIVREYGKKVKTQ

Sequences:

>Translated_350_residues
MYRMCKDSVNNQIFVKMCDIFLHVYERYFITMKNGGTNMLKKVFAMSTTVVLAAGLLSGCGTKESSANKNADTKKGYVPK
TLNVQFVPSQNADTLEAKAKPLEKLLSDKLNIPVKVSISTNYNTIVEAIASKQVDVGFLPPTAYVLAHEKKAANVILQAQ
RFGVDDETGAPTKDLVDFYKSEFVVKKDSNINSVKDLKGKKIGYQDVTSSAGYVWPAAVLLKEGVDPLKDVKPVTLKGHD
QSLIALLNGDVDAAVVFQDARNIVKKDYPNIFDQTKIVKFTEKIPNDTISVRSDLDEEWSKKLQDAFIEIGKNEEGHKII
KEVYSHEGYVKSDDSKFDIVREYGKKVKTQ
>Mature_350_residues
MYRMCKDSVNNQIFVKMCDIFLHVYERYFITMKNGGTNMLKKVFAMSTTVVLAAGLLSGCGTKESSANKNADTKKGYVPK
TLNVQFVPSQNADTLEAKAKPLEKLLSDKLNIPVKVSISTNYNTIVEAIASKQVDVGFLPPTAYVLAHEKKAANVILQAQ
RFGVDDETGAPTKDLVDFYKSEFVVKKDSNINSVKDLKGKKIGYQDVTSSAGYVWPAAVLLKEGVDPLKDVKPVTLKGHD
QSLIALLNGDVDAAVVFQDARNIVKKDYPNIFDQTKIVKFTEKIPNDTISVRSDLDEEWSKKLQDAFIEIGKNEEGHKII
KEVYSHEGYVKSDDSKFDIVREYGKKVKTQ

Specific function: Part of the ABC transporter complex phnCDE involved in phosphate import. Responsible for phosphate binding [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790543, Length=267, Percent_Identity=24.3445692883895, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 38977; Mature: 38977

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRMCKDSVNNQIFVKMCDIFLHVYERYFITMKNGGTNMLKKVFAMSTTVVLAAGLLSGC
CCCCCHHHCCCEEHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
GTKESSANKNADTKKGYVPKTLNVQFVPSQNADTLEAKAKPLEKLLSDKLNIPVKVSIST
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECC
NYNTIVEAIASKQVDVGFLPPTAYVLAHEKKAANVILQAQRFGVDDETGAPTKDLVDFYK
CHHHHHHHHHHCCCCCCCCCCHHHEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
SEFVVKKDSNINSVKDLKGKKIGYQDVTSSAGYVWPAAVLLKEGVDPLKDVKPVTLKGHD
HCEEEECCCCCCHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCHHHHCCCEEEECCC
QSLIALLNGDVDAAVVFQDARNIVKKDYPNIFDQTKIVKFTEKIPNDTISVRSDLDEEWS
CEEEEEECCCCCCEEEEHHHHHHHHHCCCCHHCCHHHHHHHHHCCCCCCHHHHCCHHHHH
KKLQDAFIEIGKNEEGHKIIKEVYSHEGYVKSDDSKFDIVREYGKKVKTQ
HHHHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYRMCKDSVNNQIFVKMCDIFLHVYERYFITMKNGGTNMLKKVFAMSTTVVLAAGLLSGC
CCCCCHHHCCCEEHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
GTKESSANKNADTKKGYVPKTLNVQFVPSQNADTLEAKAKPLEKLLSDKLNIPVKVSIST
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECC
NYNTIVEAIASKQVDVGFLPPTAYVLAHEKKAANVILQAQRFGVDDETGAPTKDLVDFYK
CHHHHHHHHHHCCCCCCCCCCHHHEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
SEFVVKKDSNINSVKDLKGKKIGYQDVTSSAGYVWPAAVLLKEGVDPLKDVKPVTLKGHD
HCEEEECCCCCCHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCHHHHCCCEEEECCC
QSLIALLNGDVDAAVVFQDARNIVKKDYPNIFDQTKIVKFTEKIPNDTISVRSDLDEEWS
CEEEEEECCCCCCEEEEHHHHHHHHHCCCCHHCCHHHHHHHHHCCCCCCHHHHCCHHHHH
KKLQDAFIEIGKNEEGHKIIKEVYSHEGYVKSDDSKFDIVREYGKKVKTQ
HHHHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA