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The map label for this gene is yfkN [H]

Identifier: 52141852

GI number: 52141852

Start: 3529006

End: 3530589

Strand: Direct

Name: yfkN [H]

Synonym: BCZK3393

Alternate gene names: 52141852

Gene position: 3529006-3530589 (Clockwise)

Preceding gene: 52141865

Following gene: 52141849

Centisome position: 66.57

GC content: 33.46

Gene sequence:

>1584_bases
TTGAATCCAAATCAAATTGTGACTTTAAACATCTTATTAACAAGTGATATACATGGTACTGTTTATCCAATTCACTACCA
AGATAACTCCAAAGCTGAAATTGGTTTAGCTAAAATTTCTACTCTTATAAAAAAAGAACGTTCCAAAAATAGAAACGTTC
TTTTAATTGATAACGGTGACTTCATACAAGGAACACCATTTACTTATCATTACGCTACTTATGAAAAAGATAAGAAAAAT
CCAATGTCTTTATTGGCGAACTATTTACGTTATGATGCTGCAGTCATTGGGAATCATGAATTTAATTATGGAATGGACGT
ATTAAACAATGTTATTTCTACTGCAAACTTCCCGTATTTATCAGCAAATATTTTAGATAAAAACAGTAAAAAACCTTATT
TCGGGAAACCATATACAATAAAACATATTGAATCAAATATAAAAATTGCTATTTTAGGGGTGACAACGCATTATATTCCA
AATTGGGAACAACCCGATCACATTAAAGACTTACAATTTGAAGATGCATTAGAAACAACACAAAATTGGGTTTCTTACAT
TCGTGAACACGAGAAACCAGATTTACTAGTCGTAGCGTATCATGGGGGATTTGAACGGGATTTACAAACCGGAGTACCGA
CTGAAGTTTTAACAGGAGAAAACCAAGGATATGCAATGTGCCATGAAATTGAAGGCATAGATATTTTGCTAACAGGCCAC
CAACACCGGGAAATCGCTCACACCGTAATAAATGGTGTAACAGTATTACAAACCGGATGCAATGGGCACTTCGTTGGAAA
AGTAACAATAACATTCGAAAAAACAGAACAAAAATGGATAAAAAGAGAGAGCTATTCACAATTATTGCCCGTACAAGGAA
TTGCTACGGACCAAGATACTCTTTCTTTGGTTACTGATTATGAAGAGAAAACACAGAATTGGCTCGATCAACCTATTGGA
CTAATCTATGGGGACATGCAAATTTCAGATGCTATGCAAACTCGTTTGCAAGACCATCCTTTTATTGAATTCATAAATAA
AATACAGATGGATATAGCTAATGTTTCCATTTCTTGTACAAGCTTATTCCATAATACATCTCCAGGTTTTCCAAAATATG
TAACAATGCGCGACATCGTTTCTAATTATATTTATCCAAATACATTAAAAGTAATTAGAATAACTGGAGCGGATATAAAA
GATGCTCTCGAACTCTCTGCTTCTTATTTCACATTAAAAGCAGATGGAACCATCATCGTAAACCCTAGCTTTATAGATCC
AAAACCTCAACACTATAATTACGATATGTGGGAAGGGATTTCATACGTATTAAATATTTCCAAACCAATTGGCGAAAGAG
TAGAATCTTTACAGTATAAAAGCACTCCTCTTAATATGAATGAAGAATATGAAGTCGTTATGAATAATTATCGCGCAAGC
GGCGGAGGGAACTTCTTTATGTTTCAAAATAAGCCTGTAATAAAAGACATACCAACAGATATGTCTGAACTTATCGCTAA
CTATATATTGAAGCACAAAAAGATAGAAGCAACGGTTAATAACAACTGGAAAGTTATTAACTAA

Upstream 100 bases:

>100_bases
ATTTACAGAATCTTTACACATTCTATACACTCACATTAAAATTCCCTTCATATATTTGATATGCTTAATTAGTAGATAAC
AATAGAAAAGGTGAATAATA

Downstream 100 bases:

>100_bases
CGATTATAATAAATAAAAAAGATGGGCTTCGCCCATCTTTTAGTTTAATTTTGCATTATCTTGCTTTTTTAAAGCTTTTA
ATTTCGCCGGTGTAACATCA

Product: 5'-nucleotidase; 2',3'-cyclic phosphodiesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MNPNQIVTLNILLTSDIHGTVYPIHYQDNSKAEIGLAKISTLIKKERSKNRNVLLIDNGDFIQGTPFTYHYATYEKDKKN
PMSLLANYLRYDAAVIGNHEFNYGMDVLNNVISTANFPYLSANILDKNSKKPYFGKPYTIKHIESNIKIAILGVTTHYIP
NWEQPDHIKDLQFEDALETTQNWVSYIREHEKPDLLVVAYHGGFERDLQTGVPTEVLTGENQGYAMCHEIEGIDILLTGH
QHREIAHTVINGVTVLQTGCNGHFVGKVTITFEKTEQKWIKRESYSQLLPVQGIATDQDTLSLVTDYEEKTQNWLDQPIG
LIYGDMQISDAMQTRLQDHPFIEFINKIQMDIANVSISCTSLFHNTSPGFPKYVTMRDIVSNYIYPNTLKVIRITGADIK
DALELSASYFTLKADGTIIVNPSFIDPKPQHYNYDMWEGISYVLNISKPIGERVESLQYKSTPLNMNEEYEVVMNNYRAS
GGGNFFMFQNKPVIKDIPTDMSELIANYILKHKKIEATVNNNWKVIN

Sequences:

>Translated_527_residues
MNPNQIVTLNILLTSDIHGTVYPIHYQDNSKAEIGLAKISTLIKKERSKNRNVLLIDNGDFIQGTPFTYHYATYEKDKKN
PMSLLANYLRYDAAVIGNHEFNYGMDVLNNVISTANFPYLSANILDKNSKKPYFGKPYTIKHIESNIKIAILGVTTHYIP
NWEQPDHIKDLQFEDALETTQNWVSYIREHEKPDLLVVAYHGGFERDLQTGVPTEVLTGENQGYAMCHEIEGIDILLTGH
QHREIAHTVINGVTVLQTGCNGHFVGKVTITFEKTEQKWIKRESYSQLLPVQGIATDQDTLSLVTDYEEKTQNWLDQPIG
LIYGDMQISDAMQTRLQDHPFIEFINKIQMDIANVSISCTSLFHNTSPGFPKYVTMRDIVSNYIYPNTLKVIRITGADIK
DALELSASYFTLKADGTIIVNPSFIDPKPQHYNYDMWEGISYVLNISKPIGERVESLQYKSTPLNMNEEYEVVMNNYRAS
GGGNFFMFQNKPVIKDIPTDMSELIANYILKHKKIEATVNNNWKVIN
>Mature_527_residues
MNPNQIVTLNILLTSDIHGTVYPIHYQDNSKAEIGLAKISTLIKKERSKNRNVLLIDNGDFIQGTPFTYHYATYEKDKKN
PMSLLANYLRYDAAVIGNHEFNYGMDVLNNVISTANFPYLSANILDKNSKKPYFGKPYTIKHIESNIKIAILGVTTHYIP
NWEQPDHIKDLQFEDALETTQNWVSYIREHEKPDLLVVAYHGGFERDLQTGVPTEVLTGENQGYAMCHEIEGIDILLTGH
QHREIAHTVINGVTVLQTGCNGHFVGKVTITFEKTEQKWIKRESYSQLLPVQGIATDQDTLSLVTDYEEKTQNWLDQPIG
LIYGDMQISDAMQTRLQDHPFIEFINKIQMDIANVSISCTSLFHNTSPGFPKYVTMRDIVSNYIYPNTLKVIRITGADIK
DALELSASYFTLKADGTIIVNPSFIDPKPQHYNYDMWEGISYVLNISKPIGERVESLQYKSTPLNMNEEYEVVMNNYRAS
GGGNFFMFQNKPVIKDIPTDMSELIANYILKHKKIEATVNNNWKVIN

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=559, Percent_Identity=24.3291592128801, Blast_Score=97, Evalue=5e-20,
Organism=Escherichia coli, GI1790658, Length=596, Percent_Identity=27.0134228187919, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1786687, Length=565, Percent_Identity=24.2477876106195, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19921980, Length=486, Percent_Identity=21.6049382716049, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24652512, Length=486, Percent_Identity=21.6049382716049, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI161076508, Length=486, Percent_Identity=21.6049382716049, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 60110; Mature: 60110

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPNQIVTLNILLTSDIHGTVYPIHYQDNSKAEIGLAKISTLIKKERSKNRNVLLIDNGD
CCCCEEEEEEEEEEECCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHCCCCCEEEEECCC
FIQGTPFTYHYATYEKDKKNPMSLLANYLRYDAAVIGNHEFNYGMDVLNNVISTANFPYL
EEECCCEEEEEEECCCCCCCHHHHHHHHHHHCEEEECCCCCCCCHHHHHHHHHHCCCCEE
SANILDKNSKKPYFGKPYTIKHIESNIKIAILGVTTHYIPNWEQPDHIKDLQFEDALETT
EEEEECCCCCCCCCCCCEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
QNWVSYIREHEKPDLLVVAYHGGFERDLQTGVPTEVLTGENQGYAMCHEIEGIDILLTGH
HHHHHHHHHCCCCCEEEEEECCCCCHHHHCCCCCCEEECCCCCEEEEEECCCEEEEEECC
QHREIAHTVINGVTVLQTGCNGHFVGKVTITFEKTEQKWIKRESYSQLLPVQGIATDQDT
CHHHHHHHHHCCEEEEEECCCCEEEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCHH
LSLVTDYEEKTQNWLDQPIGLIYGDMQISDAMQTRLQDHPFIEFINKIQMDIANVSISCT
HHHHHCHHHHHHHHHCCCCEEEEECEEHHHHHHHHCCCCHHHHHHHHHHHHHHCEEEEEE
SLFHNTSPGFPKYVTMRDIVSNYIYPNTLKVIRITGADIKDALELSASYFTLKADGTIIV
EEECCCCCCCCCEEEHHHHHHHCCCCCCEEEEEEECCCHHHHHHCCCEEEEEEECCEEEE
NPSFIDPKPQHYNYDMWEGISYVLNISKPIGERVESLQYKSTPLNMNEEYEVVMNNYRAS
CCCCCCCCCCCCCCHHHHCCEEEEECCHHHHHHHHHCEECCCCCCCCCCHHHEEECEECC
GGGNFFMFQNKPVIKDIPTDMSELIANYILKHKKIEATVNNNWKVIN
CCCCEEEECCCCCHHCCCCCHHHHHHHHHHHCCEEEEEECCCEEEEC
>Mature Secondary Structure
MNPNQIVTLNILLTSDIHGTVYPIHYQDNSKAEIGLAKISTLIKKERSKNRNVLLIDNGD
CCCCEEEEEEEEEEECCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHCCCCCEEEEECCC
FIQGTPFTYHYATYEKDKKNPMSLLANYLRYDAAVIGNHEFNYGMDVLNNVISTANFPYL
EEECCCEEEEEEECCCCCCCHHHHHHHHHHHCEEEECCCCCCCCHHHHHHHHHHCCCCEE
SANILDKNSKKPYFGKPYTIKHIESNIKIAILGVTTHYIPNWEQPDHIKDLQFEDALETT
EEEEECCCCCCCCCCCCEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
QNWVSYIREHEKPDLLVVAYHGGFERDLQTGVPTEVLTGENQGYAMCHEIEGIDILLTGH
HHHHHHHHHCCCCCEEEEEECCCCCHHHHCCCCCCEEECCCCCEEEEEECCCEEEEEECC
QHREIAHTVINGVTVLQTGCNGHFVGKVTITFEKTEQKWIKRESYSQLLPVQGIATDQDT
CHHHHHHHHHCCEEEEEECCCCEEEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCHH
LSLVTDYEEKTQNWLDQPIGLIYGDMQISDAMQTRLQDHPFIEFINKIQMDIANVSISCT
HHHHHCHHHHHHHHHCCCCEEEEECEEHHHHHHHHCCCCHHHHHHHHHHHHHHCEEEEEE
SLFHNTSPGFPKYVTMRDIVSNYIYPNTLKVIRITGADIKDALELSASYFTLKADGTIIV
EEECCCCCCCCCEEEHHHHHHHCCCCCCEEEEEEECCCHHHHHHCCCEEEEEEECCEEEE
NPSFIDPKPQHYNYDMWEGISYVLNISKPIGERVESLQYKSTPLNMNEEYEVVMNNYRAS
CCCCCCCCCCCCCCHHHHCCEEEEECCHHHHHHHHHCEECCCCCCCCCCHHHEEECEECC
GGGNFFMFQNKPVIKDIPTDMSELIANYILKHKKIEATVNNNWKVIN
CCCCEEEECCCCCHHCCCCCHHHHHHHHHHHCCEEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]