Click here to switch to the map view.

The map label for this gene is ogt [H]

Identifier: 52141749

GI number: 52141749

Start: 3622937

End: 3623467

Strand: Direct

Name: ogt [H]

Synonym: BCZK3498

Alternate gene names: 52141749

Gene position: 3622937-3623467 (Clockwise)

Preceding gene: 52141750

Following gene: 52141748

Centisome position: 68.35

GC content: 34.27

Gene sequence:

>531_bases
ATGAATTCTTACAAAAATAATTATATATATTGGACGCTACTTACACATGCAAATTGGAAGTTTCATATTGCTGCAACTGA
AACTGGACTATGTTTCATTGGATCACAAAACGAAACCTTTGAAGAAGTAAATATATGGGCTAGAAAAAAACTGCCTCAAC
ATATATTGATCCATAGTCCAGATTACTTACAAGTATATACAAAAGAAATTATCGAGTATTTAGAAAACAAGAGAGAAACC
TTTACATCCCCTATCGATGCTTACGGAACTGCATTTCAATTATCTGTTTGGAGTACGGTACGTGAAATTCCTTATGGAAA
AACATATTCTTATACAGAAATTGCAGATAGAATTCAAAAACCTACAGCGGTACGTGCCGTTGCTTCTGCTATTGCTGCCA
ACCCAATTCTCATTACGATTCCTTGCCACCGTGTTATTGGAAAGAATGGTAAACTAACTGGTTTTAGAGGTGGATTAGAA
ATGAAGAAAGAATTGCTTGCATTAGAAAAATTACAGGTGGAATTCATATGA

Upstream 100 bases:

>100_bases
ATAGAAAACCCAGAATATTTCGCAACTTTATTTAAAAAGAAAACTGGGTTTACTCCTACTGAATATCGAAAAAAGAACGA
AATGAAAGAGGGATACAATA

Downstream 100 bases:

>100_bases
CCGCCGAATATAGTAACGCATTAACTTTAACAGTTCCAACAGAATTTAGTTTTCAAGAAAATTTACGCTATCTATCCCGT
TCTAGCAATGAATGTATGTT

Product: methylated-DNA--protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MNSYKNNYIYWTLLTHANWKFHIAATETGLCFIGSQNETFEEVNIWARKKLPQHILIHSPDYLQVYTKEIIEYLENKRET
FTSPIDAYGTAFQLSVWSTVREIPYGKTYSYTEIADRIQKPTAVRAVASAIAANPILITIPCHRVIGKNGKLTGFRGGLE
MKKELLALEKLQVEFI

Sequences:

>Translated_176_residues
MNSYKNNYIYWTLLTHANWKFHIAATETGLCFIGSQNETFEEVNIWARKKLPQHILIHSPDYLQVYTKEIIEYLENKRET
FTSPIDAYGTAFQLSVWSTVREIPYGKTYSYTEIADRIQKPTAVRAVASAIAANPILITIPCHRVIGKNGKLTGFRGGLE
MKKELLALEKLQVEFI
>Mature_176_residues
MNSYKNNYIYWTLLTHANWKFHIAATETGLCFIGSQNETFEEVNIWARKKLPQHILIHSPDYLQVYTKEIIEYLENKRET
FTSPIDAYGTAFQLSVWSTVREIPYGKTYSYTEIADRIQKPTAVRAVASAIAANPILITIPCHRVIGKNGKLTGFRGGLE
MKKELLALEKLQVEFI

Specific function: Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction:the enzyme is irreversibly inactivated [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=112, Percent_Identity=30.3571428571429, Blast_Score=68, Evalue=5e-12,
Organism=Escherichia coli, GI1788542, Length=152, Percent_Identity=40.1315789473684, Blast_Score=110, Evalue=5e-26,
Organism=Escherichia coli, GI1787596, Length=88, Percent_Identity=47.7272727272727, Blast_Score=94, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI115533068, Length=86, Percent_Identity=48.8372093023256, Blast_Score=91, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI115533070, Length=86, Percent_Identity=48.8372093023256, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320001, Length=113, Percent_Identity=38.9380530973451, Blast_Score=85, Evalue=6e-18,
Organism=Drosophila melanogaster, GI17137554, Length=142, Percent_Identity=38.7323943661972, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20217; Mature: 20217

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSYKNNYIYWTLLTHANWKFHIAATETGLCFIGSQNETFEEVNIWARKKLPQHILIHSP
CCCCCCCEEEEEEEECCCEEEEEEEECCCEEEECCCCCCHHHHHHHHHHCCCCEEEEECC
DYLQVYTKEIIEYLENKRETFTSPIDAYGTAFQLSVWSTVREIPYGKTYSYTEIADRIQK
CHHHHHHHHHHHHHHCCHHHHCCCHHHCCCEEEEHHHHHHHHCCCCCCCCHHHHHHHHCC
PTAVRAVASAIAANPILITIPCHRVIGKNGKLTGFRGGLEMKKELLALEKLQVEFI
CHHHHHHHHHHHCCCEEEEECCHHHHCCCCCEEEECCCHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MNSYKNNYIYWTLLTHANWKFHIAATETGLCFIGSQNETFEEVNIWARKKLPQHILIHSP
CCCCCCCEEEEEEEECCCEEEEEEEECCCEEEECCCCCCHHHHHHHHHHCCCCEEEEECC
DYLQVYTKEIIEYLENKRETFTSPIDAYGTAFQLSVWSTVREIPYGKTYSYTEIADRIQK
CHHHHHHHHHHHHHHCCHHHHCCCHHHCCCEEEEHHHHHHHHCCCCCCCCHHHHHHHHCC
PTAVRAVASAIAANPILITIPCHRVIGKNGKLTGFRGGLEMKKELLALEKLQVEFI
CHHHHHHHHHHHCCCEEEEECCHHHHCCCCCEEEECCCHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]