| Definition | Bacillus cereus E33L, complete genome. |
|---|---|
| Accession | NC_006274 |
| Length | 5,300,915 |
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The map label for this gene is priA [H]
Identifier: 52141622
GI number: 52141622
Start: 3764576
End: 3766981
Strand: Reverse
Name: priA [H]
Synonym: BCZK3627
Alternate gene names: 52141622
Gene position: 3766981-3764576 (Counterclockwise)
Preceding gene: 52141619
Following gene: 52141621
Centisome position: 71.06
GC content: 37.28
Gene sequence:
>2406_bases ATGAAATTTGCAAGTGTAATTGTTGATGTACCTGCACGTCAGACAGATCGACCATTTGATTATATTATCCCTAAAAAATG GGAAGATATTGTCCAGACGGGCATGCGTGTAGTAGTTCCGTTTGGCCCAAGGAAATTGCAAGGTTTTATTATTGGGATTA AAAATTCGGTTGAAGTAGAAAGTAAAAAGTTAAAGACAATCCACGAAATTTTAGATGTAACACCAGTTTTAAATGAAGAG TTGTTAAAGCTTGGGTATTGGCTTACAAGTGAAACGTTATGTTATATGATTTCAGCTTTTCAAGTTATGCTTCCGACGGC GATAAAAGCAACATATAAAAAACGTCTACAGCTTCGTAAACAAGAAGAAGTAGCACCTGAACTACTATTTTTATTTCAGG ACAAAGAAGCGATAGATTGGGATGCGATTGAGACGCAACCACATCTATACCGCACGATTCAACAAGAAATTAAACATGGC ACGATTGAAGTCGTTTATCAAGTAAAAGATAAAGTACAAAAGAAGAAACAAAGAGTCGTTCAACCGGAGTTGCCAGAAGA TAAATTAGAATTGGCAGCATTTGAACTGAAAAGTAAAAAACAACAAGATGTCCTCTATTATTTTGTGGAAAATTATAAAA GTGTGCCGTTGAAAGTAATAACAGAAGAGTTGCAAATAACAGATGCGCCGATTAAAGCACTTGTTAAAAAGGGGCTAATC TCAGAAAAGTATGTGGAAGTATATCGGAATCCGTATGACGATGACGATTTTGAACAAACGAAACCATTCCCACTTACTGA GGAACAAAAGCAAGTTATTACACCGATTTTATCATCAATTACAAATGAAACTTACAATCCATTTTTACTATATGGTGTTA CAGGAAGCGGAAAAACAGAAGTATATTTACAATCTATAGCAGCGGTGCTCGAGAAAGGAAAAGAAGCTATTGTACTTGTT CCTGAAATTGCACTAACGCCTCAGATGGTAGATCGTTTTAAAGGTAGATTTGGCTCACAAGTTGCGGTTCTTCATAGTGC GCTGTCTGTTGGAGAAAAATATGATGAATGGCGTAAGATTTTAAGAAAAGAAGTGAAAGTTGTAGTTGGTGCACGTTCAG CTGTATTTGCTCCTTTTGAAAATTTAGGGATTATTATTATTGATGAGGAACATGAATCGAGTTATAAGCAAGAAGATAAT CCGAGGTATCATGCAAGGGATGTAGCTGTGTGGAGGGGACAATATCATAAATGTCCTATCGTTCTTGGTAGTGCAACACC GACGCTTGAATCATTTGCAAGAGCGAAAAAAGGTGTGTATGAATTACTGACGATGGAAAAACGTATGAACGAACAAGCTT TACCGACAGTAGAGATTGTTGATATGCGTGAAGAACTTCGTGACGGGAATCGCTCCATGTTTTCAAAGGCACTGCATGAA AAAATAGCAGATCGATTAGAAAAGAAAGAACAAATGGTGCTCTTTTTAAATAGAAGGGGGCATTCTACATTTGTTATGTG CCGTGATTGCGGTTACGTTGTACAATGTCCGCATTGTGATATCTCACTAACATATCATAAAATGAACCATCGTTTAAAAT GCCATTATTGCAGTTACGAAGAGAATATGCCGACTGCGTGTCCTGCTTGTCAAAGTACATATATTCGTTTCTTTGGTACG GGTACGCAAAAGGTAGAAGAAGAAATTACAAAACTATTTCCAGAGGCACGAGTCATTCGAATGGATGTAGATACGACGAG TCGTAAAGGGATGCATGAGAAATTATTAAAGGCGTTCGGGGAAGAAAAAGCAGATATATTACTTGGAACACAAATGATTG CGAAAGGTTTAGATTTTCCGAAAGTAACGCTTGTTGGGGTTTTAACTGCTGATACAATGCTTCATTTACCTGATTTTAGG GCGAGTGAAAAGACTTATCAGTTATTGACGCAAGTAAGTGGACGAGCAGGCCGACATGAATTGCCAGGGGAAGTCATAAT TCAAACGTATACACCAGAACATTACAGTATAGAGTTAGCAAAGAACCAACAATACGATGTGTTTTTTGACCAAGAAATGC AAATGAGACGAACGAGACAATATCCACCTTATTACTATGTTGTACTTGTGACGGTATCTCATCCGGAATTATTAAAGGCA GTACAAGTGACGGAAAAAATTGTCGGTCATTTACGATCGCACTGTTCGCAGCAAACAATGGTGTTAGGACCGGTTGCTTC AGCAATTCCAAGGATAAAAGATAGATATCGTTATCAATGCATGATAAAATACAAACGGGAACCAAACTTAAAGAACGTGC TCAAAATGGTAAATGAACATTATCAAGCAGAAATGCAAAAAGAGCTACAAATCTCAATTGATTTTAATCCAACAATGTTA ATGTAG
Upstream 100 bases:
>100_bases GAAAAGATGGAGAAGTTATAGAGTTGCCACTTTTAACGAAGAAAGAGGTCGCTCGTGAAATATTAAAGCAAATTGAAATA ATGTTAGAAGATGATCGTCT
Downstream 100 bases:
>100_bases CGGAGGGAAATATGGCAGTTTTAGAAATAGTAAAGCATCCAAATGAAGTGTTAGAAACACCTTGTGAAAGAGTAATTAAC TTTGATAAAAAGTTAGTGAA
Product: primosome assembly protein PriA
Products: NA
Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]
Number of amino acids: Translated: 801; Mature: 801
Protein sequence:
>801_residues MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVESKKLKTIHEILDVTPVLNEE LLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRKQEEVAPELLFLFQDKEAIDWDAIETQPHLYRTIQQEIKHG TIEVVYQVKDKVQKKKQRVVQPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTEVYLQSIAAVLEKGKEAIVLV PEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKILRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDN PRYHARDVAVWRGQYHKCPIVLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYEENMPTACPACQSTYIRFFGT GTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFGEEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFR ASEKTYQLLTQVSGRAGRHELPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEHYQAEMQKELQISIDFNPTML M
Sequences:
>Translated_801_residues MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVESKKLKTIHEILDVTPVLNEE LLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRKQEEVAPELLFLFQDKEAIDWDAIETQPHLYRTIQQEIKHG TIEVVYQVKDKVQKKKQRVVQPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTEVYLQSIAAVLEKGKEAIVLV PEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKILRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDN PRYHARDVAVWRGQYHKCPIVLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYEENMPTACPACQSTYIRFFGT GTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFGEEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFR ASEKTYQLLTQVSGRAGRHELPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEHYQAEMQKELQISIDFNPTML M >Mature_801_residues MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVESKKLKTIHEILDVTPVLNEE LLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRKQEEVAPELLFLFQDKEAIDWDAIETQPHLYRTIQQEIKHG TIEVVYQVKDKVQKKKQRVVQPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTEVYLQSIAAVLEKGKEAIVLV PEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKILRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDN PRYHARDVAVWRGQYHKCPIVLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYEENMPTACPACQSTYIRFFGT GTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFGEEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFR ASEKTYQLLTQVSGRAGRHELPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEHYQAEMQKELQISIDFNPTML M
Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase
COG id: COG1198
COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 helicase C-terminal domain [H]
Homologues:
Organism=Escherichia coli, GI1790370, Length=556, Percent_Identity=39.568345323741, Blast_Score=421, Evalue=1e-119, Organism=Escherichia coli, GI1787357, Length=191, Percent_Identity=25.130890052356, Blast_Score=65, Evalue=2e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014001 - InterPro: IPR011545 - InterPro: IPR001650 - InterPro: IPR014021 - InterPro: IPR005259 [H]
Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]
EC number: NA
Molecular weight: Translated: 92598; Mature: 92598
Theoretical pI: Translated: 8.25; Mature: 8.25
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.5 %Cys (Translated Protein) 3.1 %Met (Translated Protein) 4.6 %Cys+Met (Translated Protein) 1.5 %Cys (Mature Protein) 3.1 %Met (Mature Protein) 4.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVE CCHHHEEEECCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCEEEEEEEEECCCCCHH SKKLKTIHEILDVTPVLNEELLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRK HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH QEEVAPELLFLFQDKEAIDWDAIETQPHLYRTIQQEIKHGTIEVVYQVKDKVQKKKQRVV HHHHCHHHEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHCC QPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCC SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTE HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH VYLQSIAAVLEKGKEAIVLVPEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKI HHHHHHHHHHHCCCCEEEEECCHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH LRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDNPRYHARDVAVWRGQYHKCPI HHHHHHHHCCCCHHEECCHHHCCEEEEECCCCCCCCCCCCCCEEHHHHHEECCCCCCCCE VLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE EECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCCHHHHHHHHHH KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYE HHHHHHHHHCCEEEEEECCCCCEEEEEECCCCEEECCCCCEEEEEECCCCCEEEEEECCC ENMPTACPACQSTYIRFFGTGTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFG CCCCCCCCHHHHCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHC EEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFRASEKTYQLLTQVSGRAGRHE CCCHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCC LPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA CCCCEEEEEECCCCEEEEEECCCCEEEEECHHHHHHHHCCCCCEEEEEEEEECCHHHHHH VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEH HHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHH YQAEMQKELQISIDFNPTMLM HHHHHCCCEEEEEECCCCCCC >Mature Secondary Structure MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVE CCHHHEEEECCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCEEEEEEEEECCCCCHH SKKLKTIHEILDVTPVLNEELLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRK HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH QEEVAPELLFLFQDKEAIDWDAIETQPHLYRTIQQEIKHGTIEVVYQVKDKVQKKKQRVV HHHHCHHHEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHCC QPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCC SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTE HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH VYLQSIAAVLEKGKEAIVLVPEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKI HHHHHHHHHHHCCCCEEEEECCHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH LRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDNPRYHARDVAVWRGQYHKCPI HHHHHHHHCCCCHHEECCHHHCCEEEEECCCCCCCCCCCCCCEEHHHHHEECCCCCCCCE VLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE EECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCCHHHHHHHHHH KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYE HHHHHHHHHCCEEEEEECCCCCEEEEEECCCCEEECCCCCEEEEEECCCCCEEEEEECCC ENMPTACPACQSTYIRFFGTGTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFG CCCCCCCCHHHHCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHC EEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFRASEKTYQLLTQVSGRAGRHE CCCHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCC LPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA CCCCEEEEEECCCCEEEEEECCCCEEEEECHHHHHHHHCCCCCEEEEEEEEECCHHHHHH VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEH HHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHH YQAEMQKELQISIDFNPTMLM HHHHHCCCEEEEEECCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9534248; 9384377; 9086272 [H]