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The map label for this gene is carA [H]

Identifier: 52141601

GI number: 52141601

Start: 3786138

End: 3787235

Strand: Reverse

Name: carA [H]

Synonym: BCZK3646

Alternate gene names: 52141601

Gene position: 3787235-3786138 (Counterclockwise)

Preceding gene: 52141600

Following gene: 52141604

Centisome position: 71.44

GC content: 37.34

Gene sequence:

>1098_bases
ATGAAAAGACAACTTATCTTAGAAGATGGAACAGTATTAATTGGAACAGGTTTCGGAGGAGAAATCGAAAAGTCAGGTGA
GGTTGTATTTACAACAGGAATGACTGGATATCAAGAAACATTATCTGATCCATCATATTGCGGTCAAATTGTAACATTCA
CGTACCCATTAATCGGAAACTACGGCATCAACCGTGACGATTTTGAATCGATTCACCCATCTGTAAATGGTTTAATCGTA
AATGAAATTTGTGATCACCCATCAAACTTCCGTAACGAAATTTCGTTAAATGACTACTTAAAAGAAAGAAAGATCCCAGG
ATTAGCAGGGATTGATACGAGAAAATTAACGAGAAAAATTCGTCAATACGGTACATTACGCGGACGCCTATGTAACATGG
ATGCAGATGTAGAGTACATCGTGAGTCAATTAAAAGCGACAGTATTTACAGATCATGTAAAACGCGTATCAACGAAAGAT
CCATATCCAAGCCCAGGCCGCGGACATCGTGTTGTATTAGTAGACTTCGGTATGAAACATGGTATTTTAAGAGAATTAAA
TAAACGTGATTGTGATGTAATTGTAGTTCCTTACAACACGACAGCTGAAGAAATTTTACGCCTTAGCCCAGATGGAATTA
TGTTAAGTAACGGACCTGGGGATCCAAAAGATGTACCAGAAGCAATTGAAATGTTAAAAGACATTATCGGTAAAGTTCCT
TTATTCGGAATTTGCCTAGGACACCAACTATTCGCTTTAGCATCTGGTGCGAATACAAGTAAGTTAAAATTCGGTCACCG
TGGTTTAAACCATCCAGTAAAAAATATTGCAACTGGAAAAGTAGCAATTACATCTCAAAACCATGGTTACGCAGTAGAAG
AAGAATCAGTTGAAAATACAGAACTTGAAATTACACATGTTGCTTTAAATGATGGAACAGTAGAAGGTCTTCGTCATAAG
AAGTTCCCAGCATTTACAGTTCAATACCATCCAGAAGCTTCAGCAGGACCAGAAGATGCAAATGATTTATTCGAAGATTT
CTTAACAATGATTGAAAACTTCAAGAAAGAAGGGGAAGAGTTATGCCAAAACGCCTAG

Upstream 100 bases:

>100_bases
AAGGAAAAAACACACCATTCGCAGGTTGGAAATGCCAAGGATGGCCGGTAATGACAATCGTTGGTGGTAAGATCGCATGG
CAAAAGGAGAGTGCATTAGT

Downstream 100 bases:

>100_bases
ACATTAACACAATTTTAGTAATCGGATCAGGACCAATTGTAATTGGGCAAGCAGCGGAGTTTGACTACTCTGGAACACAA
GCTTGTCAATCTCTTAAAGA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKRQLILEDGTVLIGTGFGGEIEKSGEVVFTTGMTGYQETLSDPSYCGQIVTFTYPLIGNYGINRDDFESIHPSVNGLIV
NEICDHPSNFRNEISLNDYLKERKIPGLAGIDTRKLTRKIRQYGTLRGRLCNMDADVEYIVSQLKATVFTDHVKRVSTKD
PYPSPGRGHRVVLVDFGMKHGILRELNKRDCDVIVVPYNTTAEEILRLSPDGIMLSNGPGDPKDVPEAIEMLKDIIGKVP
LFGICLGHQLFALASGANTSKLKFGHRGLNHPVKNIATGKVAITSQNHGYAVEEESVENTELEITHVALNDGTVEGLRHK
KFPAFTVQYHPEASAGPEDANDLFEDFLTMIENFKKEGEELCQNA

Sequences:

>Translated_365_residues
MKRQLILEDGTVLIGTGFGGEIEKSGEVVFTTGMTGYQETLSDPSYCGQIVTFTYPLIGNYGINRDDFESIHPSVNGLIV
NEICDHPSNFRNEISLNDYLKERKIPGLAGIDTRKLTRKIRQYGTLRGRLCNMDADVEYIVSQLKATVFTDHVKRVSTKD
PYPSPGRGHRVVLVDFGMKHGILRELNKRDCDVIVVPYNTTAEEILRLSPDGIMLSNGPGDPKDVPEAIEMLKDIIGKVP
LFGICLGHQLFALASGANTSKLKFGHRGLNHPVKNIATGKVAITSQNHGYAVEEESVENTELEITHVALNDGTVEGLRHK
KFPAFTVQYHPEASAGPEDANDLFEDFLTMIENFKKEGEELCQNA
>Mature_365_residues
MKRQLILEDGTVLIGTGFGGEIEKSGEVVFTTGMTGYQETLSDPSYCGQIVTFTYPLIGNYGINRDDFESIHPSVNGLIV
NEICDHPSNFRNEISLNDYLKERKIPGLAGIDTRKLTRKIRQYGTLRGRLCNMDADVEYIVSQLKATVFTDHVKRVSTKD
PYPSPGRGHRVVLVDFGMKHGILRELNKRDCDVIVVPYNTTAEEILRLSPDGIMLSNGPGDPKDVPEAIEMLKDIIGKVP
LFGICLGHQLFALASGANTSKLKFGHRGLNHPVKNIATGKVAITSQNHGYAVEEESVENTELEITHVALNDGTVEGLRHK
KFPAFTVQYHPEASAGPEDANDLFEDFLTMIENFKKEGEELCQNA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=366, Percent_Identity=41.5300546448087, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI21361331, Length=367, Percent_Identity=36.5122615803815, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI169790915, Length=367, Percent_Identity=36.5122615803815, Blast_Score=217, Evalue=2e-56,
Organism=Escherichia coli, GI1786215, Length=379, Percent_Identity=45.3825857519789, Blast_Score=304, Evalue=7e-84,
Organism=Caenorhabditis elegans, GI193204318, Length=371, Percent_Identity=40.1617250673854, Blast_Score=253, Evalue=9e-68,
Organism=Saccharomyces cerevisiae, GI6324878, Length=377, Percent_Identity=39.2572944297082, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6322331, Length=392, Percent_Identity=37.7551020408163, Blast_Score=254, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6322638, Length=152, Percent_Identity=34.2105263157895, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45555749, Length=377, Percent_Identity=40.5835543766578, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24642586, Length=377, Percent_Identity=40.5835543766578, Blast_Score=259, Evalue=3e-69,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40391; Mature: 40391

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRQLILEDGTVLIGTGFGGEIEKSGEVVFTTGMTGYQETLSDPSYCGQIVTFTYPLIGN
CCCEEEEECCCEEEECCCCCCCCCCCCEEEEECCCCHHHHCCCHHHCCEEEEEEEHHHCC
YGINRDDFESIHPSVNGLIVNEICDHPSNFRNEISLNDYLKERKIPGLAGIDTRKLTRKI
CCCCHHHHHHHCCCCCCEEHHHHHCCCCCHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHH
RQYGTLRGRLCNMDADVEYIVSQLKATVFTDHVKRVSTKDPYPSPGRGHRVVLVDFGMKH
HHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHH
GILRELNKRDCDVIVVPYNTTAEEILRLSPDGIMLSNGPGDPKDVPEAIEMLKDIIGKVP
HHHHHHCCCCCCEEEECCCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCC
LFGICLGHQLFALASGANTSKLKFGHRGLNHPVKNIATGKVAITSQNHGYAVEEESVENT
HHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHCCCCEEEEEECCCCCEECCCCCCCC
ELEITHVALNDGTVEGLRHKKFPAFTVQYHPEASAGPEDANDLFEDFLTMIENFKKEGEE
EEEEEEEEECCCCHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LCQNA
HHCCC
>Mature Secondary Structure
MKRQLILEDGTVLIGTGFGGEIEKSGEVVFTTGMTGYQETLSDPSYCGQIVTFTYPLIGN
CCCEEEEECCCEEEECCCCCCCCCCCCEEEEECCCCHHHHCCCHHHCCEEEEEEEHHHCC
YGINRDDFESIHPSVNGLIVNEICDHPSNFRNEISLNDYLKERKIPGLAGIDTRKLTRKI
CCCCHHHHHHHCCCCCCEEHHHHHCCCCCHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHH
RQYGTLRGRLCNMDADVEYIVSQLKATVFTDHVKRVSTKDPYPSPGRGHRVVLVDFGMKH
HHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHH
GILRELNKRDCDVIVVPYNTTAEEILRLSPDGIMLSNGPGDPKDVPEAIEMLKDIIGKVP
HHHHHHCCCCCCEEEECCCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCC
LFGICLGHQLFALASGANTSKLKFGHRGLNHPVKNIATGKVAITSQNHGYAVEEESVENT
HHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHCCCCEEEEEECCCCCEECCCCCCCC
ELEITHVALNDGTVEGLRHKKFPAFTVQYHPEASAGPEDANDLFEDFLTMIENFKKEGEE
EEEEEEEEECCCCHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LCQNA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]