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The map label for this gene is def [H]

Identifier: 52141515

GI number: 52141515

Start: 3869286

End: 3869840

Strand: Direct

Name: def [H]

Synonym: BCZK3732

Alternate gene names: 52141515

Gene position: 3869286-3869840 (Clockwise)

Preceding gene: 52141525

Following gene: 52141513

Centisome position: 72.99

GC content: 36.58

Gene sequence:

>555_bases
ATGCTTACAATGAAAGATGTAATTCGCGAAGGAGATCCTATTTTGCGAAAAGTTGCAGAAGAGGTAGTAATACCAGCGAG
CAAAGAAGATACAAATACCCTTAAAGAAATGATTGAATTTGTAATAAATAGCCAAGATCCTGAAATGGCTGAAAAATATA
GTTTACGCCCTGGAATCGGATTAGCGGCTCCGCAAATCGGTATTTCAAAGAAAATGATTGCAGTTCACGTAACAGATACA
GACGGTACGTTATATAGTCATGCATTATTCAATCCAAAAATCATTAGCCATTCTGTTGAACGTACATATTTACAAAGTGG
TGAAGGCTGTCTATCAGTAGACCGTGAAGTACCTGGTTATGTACCTCGTTACACAAGAATTACAGTGAAAGCAACTTCTA
TCAACGGCGAAGAAGTAAAATTACGTTTAAAAGGTTTACCAGCAATTGTATTCCAACATGAAATTGACCATTTAAATGGT
GTTATGTTCTATGACCATATTAATAAAGAAAATCCATTTGCTGCTCCTGACGGTTCAAAACCTCTGGAGCGATAA

Upstream 100 bases:

>100_bases
ACCGACCTGTAGTAAAATGCTTTCCAAAGCGCTTTCCCTTACTTGTCGATGACTTTTTCCATCTCCTTTGGTAGACTAAG
GAGGAAAGGAGAGATTACAT

Downstream 100 bases:

>100_bases
TAAAAAAAGGCGGAAGATAATCCTCCGCCTTTTTACTATTATGAAATGGGTTCCATATCCACTTATAACAATCCAGCCCA
TTTTAATCCATGATATATGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MLTMKDVIREGDPILRKVAEEVVIPASKEDTNTLKEMIEFVINSQDPEMAEKYSLRPGIGLAAPQIGISKKMIAVHVTDT
DGTLYSHALFNPKIISHSVERTYLQSGEGCLSVDREVPGYVPRYTRITVKATSINGEEVKLRLKGLPAIVFQHEIDHLNG
VMFYDHINKENPFAAPDGSKPLER

Sequences:

>Translated_184_residues
MLTMKDVIREGDPILRKVAEEVVIPASKEDTNTLKEMIEFVINSQDPEMAEKYSLRPGIGLAAPQIGISKKMIAVHVTDT
DGTLYSHALFNPKIISHSVERTYLQSGEGCLSVDREVPGYVPRYTRITVKATSINGEEVKLRLKGLPAIVFQHEIDHLNG
VMFYDHINKENPFAAPDGSKPLER
>Mature_184_residues
MLTMKDVIREGDPILRKVAEEVVIPASKEDTNTLKEMIEFVINSQDPEMAEKYSLRPGIGLAAPQIGISKKMIAVHVTDT
DGTLYSHALFNPKIISHSVERTYLQSGEGCLSVDREVPGYVPRYTRITVKATSINGEEVKLRLKGLPAIVFQHEIDHLNG
VMFYDHINKENPFAAPDGSKPLER

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=122, Percent_Identity=36.8852459016393, Blast_Score=71, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24645728, Length=174, Percent_Identity=28.1609195402299, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20529; Mature: 20529

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTMKDVIREGDPILRKVAEEVVIPASKEDTNTLKEMIEFVINSQDPEMAEKYSLRPGIG
CCCHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
LAAPQIGISKKMIAVHVTDTDGTLYSHALFNPKIISHSVERTYLQSGEGCLSVDREVPGY
CCCCCCCCCCEEEEEEEECCCCCEEEHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCC
VPRYTRITVKATSINGEEVKLRLKGLPAIVFQHEIDHLNGVMFYDHINKENPFAAPDGSK
CCCEEEEEEEEEECCCCEEEEEECCCCEEEEEHHHHHCCCEEEEEECCCCCCCCCCCCCC
PLER
CCCC
>Mature Secondary Structure
MLTMKDVIREGDPILRKVAEEVVIPASKEDTNTLKEMIEFVINSQDPEMAEKYSLRPGIG
CCCHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
LAAPQIGISKKMIAVHVTDTDGTLYSHALFNPKIISHSVERTYLQSGEGCLSVDREVPGY
CCCCCCCCCCEEEEEEEECCCCCEEEHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCC
VPRYTRITVKATSINGEEVKLRLKGLPAIVFQHEIDHLNGVMFYDHINKENPFAAPDGSK
CCCEEEEEEEEEECCCCEEEEEECCCCEEEEEHHHHHCCCEEEEEECCCCCCCCCCCCCC
PLER
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]