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The map label for this gene is ppx [H]

Identifier: 52141496

GI number: 52141496

Start: 3887420

End: 3888958

Strand: Reverse

Name: ppx [H]

Synonym: BCZK3752

Alternate gene names: 52141496

Gene position: 3888958-3887420 (Counterclockwise)

Preceding gene: 52141493

Following gene: 52141497

Centisome position: 73.36

GC content: 33.14

Gene sequence:

>1539_bases
TTGAAAGAAATATTAAAACAGCAATATGCCATTATAGATATTGGATCTAATACAATGCGTTTAGTTATTTATGAAAAACA
AAACGGGGGTTTTTATAAAGAGATTGAAAATACGAAAGTGGTTGCAAGGTTAAGGAATTACTTAGTCGATGGCGTGTTGA
TTGAAGAAGGAATAGAGGTATTGTTACAAACATTATTTCAATTTCAAGAAAGTACACGATTCCATCAGTTGCACCATGTA
CTTTGTGTTGCAACAGCAACAATTAGACAGGCTAAGAATCAAGAGGAAATTAAAAAGCTTGTTGAAGGACAAACAGACTT
TACTCTTAGAGTATTATCAGAATATGAAGAAGCACGTTACGGCTATTTAGCAGTTATGAATTCAACTTCGTTCTCTGAAG
GGATTACAGTAGATATTGGTGGGGGGAGTACTGAGGTTACATACTTTCGAAATAGAGAGATTCTAGAGTATCATAGCTTT
CCTTTTGGAGCACTTTCTTTAAAGCAACAATTTATTAAAGACGATATACCTACTGAAGAAGAGTTAGAAAAGCTTCAAAC
GTACCTAGAATATCAATTTCGAACATTACCATGGTTAATTGATAAGAAATTGTCTCTTATTGCAATTGGTGGTAGTGCGA
GGAACTTAGTGAAAATCCATCAAAATTTAATTTGTTATCCTATAGCAGGAGTACATTTATATAAGATGAAAGAAGAAGAT
ATTAAAAATGTGAAAGAAGAATTGGAAGCATTGTCGTTCATAGAACTTCAAAAATTGGAAGGATTGGCAAAAGATCGAGC
AGATACAATTGTTCCAGCGGTCGAAGTATTTCATACGCTTGTTAATGTTATAGAGGCGCCAGCATTTGTATTAAGTAGAA
AAGGATTACGAGAAGGTGTTTTCTATGAAGAGTTGACAAAAGATTTGGGGATTTCATATTATCCGAACGTGGTCGAAGAA
AGTTTGTATTTATTATCACATGAATATGAAATGGATATGGAATTTGTAATACAGCTTATTAAGCATGGAAGATTGATTTG
TCAACAGCTTGAAGAAACAGGACTGATTTCTATGTCAGTAGAAGACTGGGAAGTATTCCATCAAGCAGCGAAAGTATTTA
ATATAGGCAAATATATAGACGAAGAAGCGAGCCGCTTACATACGTTTTATTTATTAGCGAATAAAACAATTGATGGTATG
ATGCACAAAGAGCGAGTAAGATTAGCGCTTATTGCGTCTTATAAATCAAAAATGTTATTCAAACAACATTTGTCTCCATT
TGAAGGCTGGTTTGATAAAAACGAACAAAAAAAAATCCGTCTATTAGGGGCTGTTTTACAATTTTCAGCAGCTCTAAATA
TAAGACAAAGATCGCTTGTTGAATCAATATCTATAACAGAAAGTAAAGAAGGATTAACATTTGAAATTGTATGTGAACAA
TCGGCGTTAACGGAAAAAGTGCAAGCTGAAAAGCAGAAGAAACAGCTAGAAAGAGTATTGAAAACGAATATTACCTTGTT
ATTTAAATTAAAGAATTGA

Upstream 100 bases:

>100_bases
ATATTTTCAATATTAAAGCAATATTGTTACAAAAAACAACTATTTTGTATAATAGTAATAGAATGTGTTTTAGAAATTTG
ATAATGAAAGAGTGAGAAGT

Downstream 100 bases:

>100_bases
GTTGTACGGATGTCTTTACGAAAAATTTACACTTTATAATATTGTAATTTGCTAAAATGAAAGTAACTAAATTATAGTAA
AAAAGTTTCAAGGGGAAGTG

Product: exopolyphosphatase, Ppx/GppA phosphatase

Products: NA

Alternate protein names: ExopolyPase; Metaphosphatase [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MKEILKQQYAIIDIGSNTMRLVIYEKQNGGFYKEIENTKVVARLRNYLVDGVLIEEGIEVLLQTLFQFQESTRFHQLHHV
LCVATATIRQAKNQEEIKKLVEGQTDFTLRVLSEYEEARYGYLAVMNSTSFSEGITVDIGGGSTEVTYFRNREILEYHSF
PFGALSLKQQFIKDDIPTEEELEKLQTYLEYQFRTLPWLIDKKLSLIAIGGSARNLVKIHQNLICYPIAGVHLYKMKEED
IKNVKEELEALSFIELQKLEGLAKDRADTIVPAVEVFHTLVNVIEAPAFVLSRKGLREGVFYEELTKDLGISYYPNVVEE
SLYLLSHEYEMDMEFVIQLIKHGRLICQQLEETGLISMSVEDWEVFHQAAKVFNIGKYIDEEASRLHTFYLLANKTIDGM
MHKERVRLALIASYKSKMLFKQHLSPFEGWFDKNEQKKIRLLGAVLQFSAALNIRQRSLVESISITESKEGLTFEIVCEQ
SALTEKVQAEKQKKQLERVLKTNITLLFKLKN

Sequences:

>Translated_512_residues
MKEILKQQYAIIDIGSNTMRLVIYEKQNGGFYKEIENTKVVARLRNYLVDGVLIEEGIEVLLQTLFQFQESTRFHQLHHV
LCVATATIRQAKNQEEIKKLVEGQTDFTLRVLSEYEEARYGYLAVMNSTSFSEGITVDIGGGSTEVTYFRNREILEYHSF
PFGALSLKQQFIKDDIPTEEELEKLQTYLEYQFRTLPWLIDKKLSLIAIGGSARNLVKIHQNLICYPIAGVHLYKMKEED
IKNVKEELEALSFIELQKLEGLAKDRADTIVPAVEVFHTLVNVIEAPAFVLSRKGLREGVFYEELTKDLGISYYPNVVEE
SLYLLSHEYEMDMEFVIQLIKHGRLICQQLEETGLISMSVEDWEVFHQAAKVFNIGKYIDEEASRLHTFYLLANKTIDGM
MHKERVRLALIASYKSKMLFKQHLSPFEGWFDKNEQKKIRLLGAVLQFSAALNIRQRSLVESISITESKEGLTFEIVCEQ
SALTEKVQAEKQKKQLERVLKTNITLLFKLKN
>Mature_512_residues
MKEILKQQYAIIDIGSNTMRLVIYEKQNGGFYKEIENTKVVARLRNYLVDGVLIEEGIEVLLQTLFQFQESTRFHQLHHV
LCVATATIRQAKNQEEIKKLVEGQTDFTLRVLSEYEEARYGYLAVMNSTSFSEGITVDIGGGSTEVTYFRNREILEYHSF
PFGALSLKQQFIKDDIPTEEELEKLQTYLEYQFRTLPWLIDKKLSLIAIGGSARNLVKIHQNLICYPIAGVHLYKMKEED
IKNVKEELEALSFIELQKLEGLAKDRADTIVPAVEVFHTLVNVIEAPAFVLSRKGLREGVFYEELTKDLGISYYPNVVEE
SLYLLSHEYEMDMEFVIQLIKHGRLICQQLEETGLISMSVEDWEVFHQAAKVFNIGKYIDEEASRLHTFYLLANKTIDGM
MHKERVRLALIASYKSKMLFKQHLSPFEGWFDKNEQKKIRLLGAVLQFSAALNIRQRSLVESISITESKEGLTFEIVCEQ
SALTEKVQAEKQKKQLERVLKTNITLLFKLKN

Specific function: Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate [H]

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family [H]

Homologues:

Organism=Escherichia coli, GI1788848, Length=437, Percent_Identity=25.1716247139588, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022371
- InterPro:   IPR003695 [H]

Pfam domain/function: PF02541 Ppx-GppA [H]

EC number: =3.6.1.11 [H]

Molecular weight: Translated: 59167; Mature: 59167

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEILKQQYAIIDIGSNTMRLVIYEKQNGGFYKEIENTKVVARLRNYLVDGVLIEEGIEV
CHHHHHCCEEEEEECCCEEEEEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LLQTLFQFQESTRFHQLHHVLCVATATIRQAKNQEEIKKLVEGQTDFTLRVLSEYEEARY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCC
GYLAVMNSTSFSEGITVDIGGGSTEVTYFRNREILEYHSFPFGALSLKQQFIKDDIPTEE
CEEEEEECCCCCCCEEEEECCCCEEEEEECCCCEEHHCCCCCHHHHHHHHHHHCCCCCHH
ELEKLQTYLEYQFRTLPWLIDKKLSLIAIGGSARNLVKIHQNLICYPIAGVHLYKMKEED
HHHHHHHHHHHHHHHCCHHHCCCEEEEEECCCHHHHHHHHHCEEEEEECCEEEEECCHHH
IKNVKEELEALSFIELQKLEGLAKDRADTIVPAVEVFHTLVNVIEAPAFVLSRKGLREGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHCCC
FYEELTKDLGISYYPNVVEESLYLLSHEYEMDMEFVIQLIKHGRLICQQLEETGLISMSV
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEH
EDWEVFHQAAKVFNIGKYIDEEASRLHTFYLLANKTIDGMMHKERVRLALIASYKSKMLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
KQHLSPFEGWFDKNEQKKIRLLGAVLQFSAALNIRQRSLVESISITESKEGLTFEIVCEQ
HHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
SALTEKVQAEKQKKQLERVLKTNITLLFKLKN
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure
MKEILKQQYAIIDIGSNTMRLVIYEKQNGGFYKEIENTKVVARLRNYLVDGVLIEEGIEV
CHHHHHCCEEEEEECCCEEEEEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LLQTLFQFQESTRFHQLHHVLCVATATIRQAKNQEEIKKLVEGQTDFTLRVLSEYEEARY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCC
GYLAVMNSTSFSEGITVDIGGGSTEVTYFRNREILEYHSFPFGALSLKQQFIKDDIPTEE
CEEEEEECCCCCCCEEEEECCCCEEEEEECCCCEEHHCCCCCHHHHHHHHHHHCCCCCHH
ELEKLQTYLEYQFRTLPWLIDKKLSLIAIGGSARNLVKIHQNLICYPIAGVHLYKMKEED
HHHHHHHHHHHHHHHCCHHHCCCEEEEEECCCHHHHHHHHHCEEEEEECCEEEEECCHHH
IKNVKEELEALSFIELQKLEGLAKDRADTIVPAVEVFHTLVNVIEAPAFVLSRKGLREGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHCCC
FYEELTKDLGISYYPNVVEESLYLLSHEYEMDMEFVIQLIKHGRLICQQLEETGLISMSV
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEH
EDWEVFHQAAKVFNIGKYIDEEASRLHTFYLLANKTIDGMMHKERVRLALIASYKSKMLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
KQHLSPFEGWFDKNEQKKIRLLGAVLQFSAALNIRQRSLVESISITESKEGLTFEIVCEQ
HHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
SALTEKVQAEKQKKQLERVLKTNITLLFKLKN
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]