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The map label for this gene is recN [H]

Identifier: 52141322

GI number: 52141322

Start: 4052354

End: 4054105

Strand: Reverse

Name: recN [H]

Synonym: BCZK3927

Alternate gene names: 52141322

Gene position: 4054105-4052354 (Counterclockwise)

Preceding gene: 52141321

Following gene: 52141323

Centisome position: 76.48

GC content: 36.02

Gene sequence:

>1752_bases
GTGAATGGGGCATTGTTATCGGAATTATCGATTAGAAACTTTGCTATTATTGAGGCATTAAATATTTCTTTTCAAAAAGG
ATTAACGGTTTTAAGTGGTGAAACAGGGGCCGGAAAATCGATTATTATTGATGCGATTAGTTTACTTGTAGGTGGCCGCG
GTTCGGCAGAATTTGTTCGATACGGAACAGAAAAAGCTGAGATAGAGGGGCTATTTTATGTAGAAGATGATAAGCATCCA
TGTATTGAAAAGGCAGAAGAGTTGGATATAGAAATAGAAGACGGCATGATTATTTTGAAGCGTGATATCGCTGCAAACGG
AAAAAGCGTATGTCGTGTAAATGGAAAACTAGTTACCCTTAGTGTATTAAAAGAAATTGGAAAAACACTTGTTGATATTC
ATGGACAGCACGAAACGCAAGACTTAATGAATGAAGAACGTCATCTATTTATGCTCGATCATTTTGATGGAGAGCGTATC
GTTAAACAATTGGATATATACCAAAACGTATACGCTGACTATGAGAAGTTAAAAAAACAATTGAAATCGTTAAGTGAAAA
TGAACAACAAATGGCGCATCGCTTAGATTTAATTCAATTCCAGCATGAAGAAATCCGTAAGGCGGATTTAAAGATGGATG
AAGAAAATAACTTAACTGAAGAACGATTGCAAATTTCTAATTTTGAAAAGATTTATAAAGCATTAGGTGACGCATATCGC
TCGTTAAGTGCTGATGGACAAGGGTTAGATAATGTAAGAAGTGCAATGGGACAAATGGAGAGTATTACACATTTAGACGA
AGTGTACCAAGAAAATCATGATTCCATTGCAAATAGCTATTACCTATTAGAAGAAGTTGCATATCAACTAAGAGAAAAAC
TCGATATGATGGAATATGACCCAAATCGTTTAGACGAAATTGAAACGCGTTTAAATGAAATTCGTATGCTAAAGAGAAAA
TATGGAAATACTGTAGAAGAGATATTAGCGTATGCTGATAAAATTGAACAAGAGATTTTTACGATTGAAAATAAAGACGT
TCATATTGAAACGACGAAGAAACAGTTGAAGGAATTAGAAAGTGTTATTCTGAAAGAAGCAACGTTGTTAAGTAATATGC
GTCATGAACTTGCAGAGCATCTTACAAATGCCATTCATCAGGAATTAAAAGAATTATATATGGAAAAAACAAAATTTGAA
GTGAGAATCATAAAGCGAGAAGGAAATGCGGAAGAGCCTCTTGTGGAGGGAGCACCGGTAAGGCTTACAGCGGACGGTTA
TGATCATGTGGAATTTTATATTTCAACCAATCCAGGTGAGCCGCTAAAACCACTTTCAAAGGTCGCTTCTGGCGGAGAGT
TGTCCCGTATTATTTTAGCTTTAAAAAGTATTTTTTCTAAGCATCAAGGTGTTGCATCTGTTATTTTTGATGAAGTGGAT
ACTGGTGTAAGTGGTCGGGTCGCACAGGCTATTGCGGAAAAAATTTATCGAGTATCAGTAAACTCACAAGTACTTTGTAT
TACGCACTTACCTCAAGTAGCTTCAATGGCGGATTCGCATTTATTTATCCGAAAACAAGTAGCGAATGATCGAACAATTA
CATCCGTTACCGTTTTAACTATGGAGGATAAAGTAACAGAAATAGCTCGAATGATTTCTGGTGTGGAAATTACAGATTTA
ACGACAGAACATGCGAAAGAATTACTTACGCAAGCGCATCATTTTAAACAGACAGCAGAGGCTATCCAGTAA

Upstream 100 bases:

>100_bases
GGAGCGGTTAGAAAAGTAATTTATACATACAAACATATAGTATGACTATTTGAATAAAGAACTGAGAAAAGCCCTTAATA
GGGGATTAGTTTATAACGAG

Downstream 100 bases:

>100_bases
TGGATAGCTTTTTTTCTCAAATGATGATTTCTTAATTCTTCCGGTTATAAATAAAGCATTGCAAGGAGAGATTAAAAAGT
GAAGCCAAGAGGCTGAATGT

Product: DNA repair protein

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MNGALLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTEKAEIEGLFYVEDDKHP
CIEKAEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERI
VKQLDIYQNVYADYEKLKKQLKSLSENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERLQISNFEKIYKALGDAYR
SLSADGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRLDEIETRLNEIRMLKRK
YGNTVEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVILKEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFE
VRIIKREGNAEEPLVEGAPVRLTADGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKSIFSKHQGVASVIFDEVD
TGVSGRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDKVTEIARMISGVEITDL
TTEHAKELLTQAHHFKQTAEAIQ

Sequences:

>Translated_583_residues
MNGALLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTEKAEIEGLFYVEDDKHP
CIEKAEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERI
VKQLDIYQNVYADYEKLKKQLKSLSENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERLQISNFEKIYKALGDAYR
SLSADGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRLDEIETRLNEIRMLKRK
YGNTVEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVILKEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFE
VRIIKREGNAEEPLVEGAPVRLTADGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKSIFSKHQGVASVIFDEVD
TGVSGRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDKVTEIARMISGVEITDL
TTEHAKELLTQAHHFKQTAEAIQ
>Mature_583_residues
MNGALLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTEKAEIEGLFYVEDDKHP
CIEKAEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERI
VKQLDIYQNVYADYEKLKKQLKSLSENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERLQISNFEKIYKALGDAYR
SLSADGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRLDEIETRLNEIRMLKRK
YGNTVEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVILKEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFE
VRIIKREGNAEEPLVEGAPVRLTADGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKSIFSKHQGVASVIFDEVD
TGVSGRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDKVTEIARMISGVEITDL
TTEHAKELLTQAHHFKQTAEAIQ

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=573, Percent_Identity=32.6352530541012, Blast_Score=293, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 66131; Mature: 66131

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGALLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVR
CCCCHHHHHHHCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHH
YGTEKAEIEGLFYVEDDKHPCIEKAEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTL
CCCCHHHCCEEEEEECCCCCHHHHHHHCCEEEECCEEEEEECCCCCCCCEEEECCEEEHH
SVLKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQLDIYQNVYADYEKLKKQ
HHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LKSLSENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERLQISNFEKIYKALGDAYR
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SLSADGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYD
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PNRLDEIETRLNEIRMLKRKYGNTVEEILAYADKIEQEIFTIENKDVHIETTKKQLKELE
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCEEEEHHHHHHHHHH
SVILKEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRIIKREGNAEEPLVEGAPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCE
RLTADGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKSIFSKHQGVASVIFDEVD
EEEECCCCEEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHC
TGVSGRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLT
CCCCHHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
MEDKVTEIARMISGVEITDLTTEHAKELLTQAHHFKQTAEAIQ
EHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNGALLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVR
CCCCHHHHHHHCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHH
YGTEKAEIEGLFYVEDDKHPCIEKAEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTL
CCCCHHHCCEEEEEECCCCCHHHHHHHCCEEEECCEEEEEECCCCCCCCEEEECCEEEHH
SVLKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQLDIYQNVYADYEKLKKQ
HHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LKSLSENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERLQISNFEKIYKALGDAYR
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SLSADGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYD
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PNRLDEIETRLNEIRMLKRKYGNTVEEILAYADKIEQEIFTIENKDVHIETTKKQLKELE
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCEEEEHHHHHHHHHH
SVILKEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRIIKREGNAEEPLVEGAPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCE
RLTADGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKSIFSKHQGVASVIFDEVD
EEEECCCCEEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHC
TGVSGRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLT
CCCCHHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
MEDKVTEIARMISGVEITDLTTEHAKELLTQAHHFKQTAEAIQ
EHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]