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The map label for this gene is rsmE [H]

Identifier: 52141192

GI number: 52141192

Start: 4166499

End: 4167248

Strand: Reverse

Name: rsmE [H]

Synonym: BCZK4058

Alternate gene names: 52141192

Gene position: 4167248-4166499 (Counterclockwise)

Preceding gene: 52141191

Following gene: 52141194

Centisome position: 78.61

GC content: 39.07

Gene sequence:

>750_bases
ATGCAACGTTATTTTGTAGAAGAGAAACATGTAAATGAGACAAGTATTCGCATTGTAGGTGATGACGTACATCATATCGC
AAGAGTGATGCGTATGTCAGTTGGTGATCATATTTATTGCTGTGTAAATGGAAGAACGGCAGTATGTTCAATTGATGAAA
TTACCAGTGAATTTATTAATACGGCTATTGTAGAATGGGTAGAGGCGTCAAGTGAACTTCCTGTTTTCGTGACGATTGCA
AGTGGACTACCGAAAGGAGACAAGCTAGAGTTAATTTTCCAAAAAGGAACTGAGCTTGGGGCAGCTGCATTCTTACCATT
TCAAGCATCTCGCTCTATTGTAAAATGGGATGCGAAAAAAGCTGATAAAAAAGTTGAGCGTTTAAGAAAAATTGTGAAAG
AAGCTGCGGAACAATCACATAGAAGTGAAATTCCAGAAGTACATGCTCCGGCATCATTTAAGCAATTGCTTTCAATGAGT
GGTGAGTATGATGTTTGTCTTGTTGCTTACGAAGAGGAAGCAAAACAAGGCGAGAAATCTAATTTTGCGAAAGCTTTAAC
GATGATGAAACCAGGTCAAAAGTTATTGATTGTATTTGGCCCAGAGGGCGGTTTAGCAGAGGAAGAGATTACAGTGCTTC
GTGAACATAATTTTGTACCATGTAGTTTAGGACCAAGAATTTTAAGAACAGAAACGGCGCCGTTATACGCGTTAAGTGCT
GCTTCGTATCATTTTGAATTGATGGGGTGA

Upstream 100 bases:

>100_bases
GTGAGGGACTTGCCGTTTGTTATATGAAAAAATGATAACGTTTCGTTCTTTTCGTAAGTATTGGCTTACGAAAGGAACGA
AACTCTACTAAGGTGATTAT

Downstream 100 bases:

>100_bases
TGATCAATGTCAACTGTTGCGTTCCATACGTTAGGTTGTAAAGTAAACCACTATGAAACAGAAGCCATTTGGCAATTGTT
TAAACAAGGTGGATATGAAA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MQRYFVEEKHVNETSIRIVGDDVHHIARVMRMSVGDHIYCCVNGRTAVCSIDEITSEFINTAIVEWVEASSELPVFVTIA
SGLPKGDKLELIFQKGTELGAAAFLPFQASRSIVKWDAKKADKKVERLRKIVKEAAEQSHRSEIPEVHAPASFKQLLSMS
GEYDVCLVAYEEEAKQGEKSNFAKALTMMKPGQKLLIVFGPEGGLAEEEITVLREHNFVPCSLGPRILRTETAPLYALSA
ASYHFELMG

Sequences:

>Translated_249_residues
MQRYFVEEKHVNETSIRIVGDDVHHIARVMRMSVGDHIYCCVNGRTAVCSIDEITSEFINTAIVEWVEASSELPVFVTIA
SGLPKGDKLELIFQKGTELGAAAFLPFQASRSIVKWDAKKADKKVERLRKIVKEAAEQSHRSEIPEVHAPASFKQLLSMS
GEYDVCLVAYEEEAKQGEKSNFAKALTMMKPGQKLLIVFGPEGGLAEEEITVLREHNFVPCSLGPRILRTETAPLYALSA
ASYHFELMG
>Mature_249_residues
MQRYFVEEKHVNETSIRIVGDDVHHIARVMRMSVGDHIYCCVNGRTAVCSIDEITSEFINTAIVEWVEASSELPVFVTIA
SGLPKGDKLELIFQKGTELGAAAFLPFQASRSIVKWDAKKADKKVERLRKIVKEAAEQSHRSEIPEVHAPASFKQLLSMS
GEYDVCLVAYEEEAKQGEKSNFAKALTMMKPGQKLLIVFGPEGGLAEEEITVLREHNFVPCSLGPRILRTETAPLYALSA
ASYHFELMG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=255, Percent_Identity=30.1960784313725, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27714; Mature: 27714

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRYFVEEKHVNETSIRIVGDDVHHIARVMRMSVGDHIYCCVNGRTAVCSIDEITSEFIN
CCCCCCCHHCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCEEEEEHHHHHHHHHH
TAIVEWVEASSELPVFVTIASGLPKGDKLELIFQKGTELGAAAFLPFQASRSIVKWDAKK
HHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCEEECCHHHCCCCEEHHHHH
ADKKVERLRKIVKEAAEQSHRSEIPEVHAPASFKQLLSMSGEYDVCLVAYEEEAKQGEKS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEECCHHHCCCHH
NFAKALTMMKPGQKLLIVFGPEGGLAEEEITVLREHNFVPCSLGPRILRTETAPLYALSA
HHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCEEECCCCCEEEEECCCCEEEEEE
ASYHFELMG
CCEEEEEEC
>Mature Secondary Structure
MQRYFVEEKHVNETSIRIVGDDVHHIARVMRMSVGDHIYCCVNGRTAVCSIDEITSEFIN
CCCCCCCHHCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCEEEEEHHHHHHHHHH
TAIVEWVEASSELPVFVTIASGLPKGDKLELIFQKGTELGAAAFLPFQASRSIVKWDAKK
HHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCEEECCHHHCCCCEEHHHHH
ADKKVERLRKIVKEAAEQSHRSEIPEVHAPASFKQLLSMSGEYDVCLVAYEEEAKQGEKS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEECCHHHCCCHH
NFAKALTMMKPGQKLLIVFGPEGGLAEEEITVLREHNFVPCSLGPRILRTETAPLYALSA
HHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCEEECCCCCEEEEECCCCEEEEEE
ASYHFELMG
CCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]