Click here to switch to the map view.

The map label for this gene is phoB [H]

Identifier: 52141157

GI number: 52141157

Start: 4199163

End: 4200548

Strand: Reverse

Name: phoB [H]

Synonym: BCZK4093

Alternate gene names: 52141157

Gene position: 4200548-4199163 (Counterclockwise)

Preceding gene: 52141154

Following gene: 52141159

Centisome position: 79.24

GC content: 36.72

Gene sequence:

>1386_bases
GTGAAAAAGTTTGTGAAAAAAGCATGGCCCTTTGCAGTTGTGGCATCATTAGCAGTTACTTCGGTAGCAACATGGAATTT
TACACGTTCTAGTGAAGTGAGCGCAGATGAGAACGGAAGCAATGCAAAGATTAAAAATGTTATTGTGTTAATTGGAGATG
GTATGGGCCCTTCATATATGACAGCTCACCGTTATATGAAAGATAATCCAAAAACATTCGAGATGGAATCTACAGAGTTT
GATAAACATCTTGTAGGAACACAAAAAACATATCCGGAAGATGAACATGAAAATATCACAGACTCAGCATCAGCAGCAAC
AGCTATGTCAGCAGGAATTAAAACATATAATGCGGCAATTGCAGTTGATAATGATAAAGCAGAAGTGAAAACTGTTCTTG
AGCAAGCGAAAGAGAAAGGGAAATCAACAGGGTTAGTTGCTACTTCTGAAATTACACATGCAACACCAGCTGCTTTCGGT
GCGCATGACATTAGTCGTAAAAATATGGACGCAATTGCAAATGATTATTTTGATGAGAAAATTAAAGGGAAGCATAAAGT
AGATGTTATGCTTGGCGGCGGCGTAAAAAACTTTGTTAGAAAAGATCGCAATCTTACAGAAGAATTTAAGAAATCTGGTT
ATAGTTACGTAACAGATCGAGATCAATTGTTAAATGATAAAAATGATCAAATTCTTGGTTTATTTGGACCAGGCGGTTTA
GATAAAATGATTGATCGCAATGAGAAAACACCTTCATTAGAAGAAATGACAAATGCAGCAATTGAACGTTTGAACAAAAA
TAAAAAAGGTTTCTTCTTAATGGTTGAAGGAAGTCAAATTGACTGGGCAGGACACGATAATGATATCGTTGGTGCAATGA
GTGAGATGGAAGACTTTGAAAAAGCATTTAAAGCTGCGATTGAGTTTGCGAAAAAAGATAAAAATACGTTAGTCATTGCA
ACGGCAGATCACGCTACTGGCGGATTATCTTTAGGTGCGAATGGTGAGTACAACTTTAAAGTAGATCCAATTAAAGCTGC
AAAACGCACACCTGACTTCATGGCAAATGAGATTGCAAAAGGTGCAAATGTAGAAGAAACATTGAAAAAATATATTGATT
TACAATTAACACCTGAAGAAATTAAAGCGGTAAATGATATTGCTCCTTCAAAAGATGTAACAAAGATTGATAATGCGATT
GAAGATATTTTCAATAAGCGTTCGGTTACAGGATGGACAACAGGTGGACATACAGGAGAAGATGTAAACGTATATGCATT
TGGCCCTGGTAAATACTTGTTCTCTGGCGTTCAAGAGAATACAAATCTCGCTAAACGTGTCTTTGATATTGTTGGCGGTG
GTGATCCGAATAAAGGAAGACGCTAA

Upstream 100 bases:

>100_bases
ATAAGTTAACAAAAAATTTACATATAACTAATTTTCCCTTAACAGTCTTCTATTAAATTTGATAGTGGGAAATGAATAAC
CTAATTAGGGGGACAAGACA

Downstream 100 bases:

>100_bases
TTATAAATGAGAGTGAGGCGAAATGCCTCACTTTTCTTTTTAGGAGATGGGAAGATGAGAGCTTTTTTCAGGGGGATAGG
ACCCTTTCTAATAGTTGTAT

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKKFVKKAWPFAVVASLAVTSVATWNFTRSSEVSADENGSNAKIKNVIVLIGDGMGPSYMTAHRYMKDNPKTFEMESTEF
DKHLVGTQKTYPEDEHENITDSASAATAMSAGIKTYNAAIAVDNDKAEVKTVLEQAKEKGKSTGLVATSEITHATPAAFG
AHDISRKNMDAIANDYFDEKIKGKHKVDVMLGGGVKNFVRKDRNLTEEFKKSGYSYVTDRDQLLNDKNDQILGLFGPGGL
DKMIDRNEKTPSLEEMTNAAIERLNKNKKGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFEKAFKAAIEFAKKDKNTLVIA
TADHATGGLSLGANGEYNFKVDPIKAAKRTPDFMANEIAKGANVEETLKKYIDLQLTPEEIKAVNDIAPSKDVTKIDNAI
EDIFNKRSVTGWTTGGHTGEDVNVYAFGPGKYLFSGVQENTNLAKRVFDIVGGGDPNKGRR

Sequences:

>Translated_461_residues
MKKFVKKAWPFAVVASLAVTSVATWNFTRSSEVSADENGSNAKIKNVIVLIGDGMGPSYMTAHRYMKDNPKTFEMESTEF
DKHLVGTQKTYPEDEHENITDSASAATAMSAGIKTYNAAIAVDNDKAEVKTVLEQAKEKGKSTGLVATSEITHATPAAFG
AHDISRKNMDAIANDYFDEKIKGKHKVDVMLGGGVKNFVRKDRNLTEEFKKSGYSYVTDRDQLLNDKNDQILGLFGPGGL
DKMIDRNEKTPSLEEMTNAAIERLNKNKKGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFEKAFKAAIEFAKKDKNTLVIA
TADHATGGLSLGANGEYNFKVDPIKAAKRTPDFMANEIAKGANVEETLKKYIDLQLTPEEIKAVNDIAPSKDVTKIDNAI
EDIFNKRSVTGWTTGGHTGEDVNVYAFGPGKYLFSGVQENTNLAKRVFDIVGGGDPNKGRR
>Mature_461_residues
MKKFVKKAWPFAVVASLAVTSVATWNFTRSSEVSADENGSNAKIKNVIVLIGDGMGPSYMTAHRYMKDNPKTFEMESTEF
DKHLVGTQKTYPEDEHENITDSASAATAMSAGIKTYNAAIAVDNDKAEVKTVLEQAKEKGKSTGLVATSEITHATPAAFG
AHDISRKNMDAIANDYFDEKIKGKHKVDVMLGGGVKNFVRKDRNLTEEFKKSGYSYVTDRDQLLNDKNDQILGLFGPGGL
DKMIDRNEKTPSLEEMTNAAIERLNKNKKGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFEKAFKAAIEFAKKDKNTLVIA
TADHATGGLSLGANGEYNFKVDPIKAAKRTPDFMANEIAKGANVEETLKKYIDLQLTPEEIKAVNDIAPSKDVTKIDNAI
EDIFNKRSVTGWTTGGHTGEDVNVYAFGPGKYLFSGVQENTNLAKRVFDIVGGGDPNKGRR

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=485, Percent_Identity=30.7216494845361, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI94721246, Length=485, Percent_Identity=29.8969072164948, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI157266292, Length=452, Percent_Identity=30.5309734513274, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI116734717, Length=453, Percent_Identity=30.242825607064, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI294660770, Length=448, Percent_Identity=29.6875, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI294660772, Length=393, Percent_Identity=28.7531806615776, Blast_Score=117, Evalue=3e-26,
Organism=Escherichia coli, GI48994877, Length=340, Percent_Identity=38.5294117647059, Blast_Score=179, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6320689, Length=449, Percent_Identity=34.7438752783964, Blast_Score=200, Evalue=3e-52,
Organism=Drosophila melanogaster, GI21355981, Length=453, Percent_Identity=30.9050772626932, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI18859923, Length=415, Percent_Identity=28.9156626506024, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21355151, Length=451, Percent_Identity=29.0465631929047, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19921912, Length=469, Percent_Identity=29.2110874200426, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI85725200, Length=444, Percent_Identity=29.7297297297297, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI85815833, Length=444, Percent_Identity=29.7297297297297, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24651554, Length=491, Percent_Identity=29.5315682281059, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21358067, Length=451, Percent_Identity=29.2682926829268, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24657835, Length=453, Percent_Identity=28.9183222958057, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21355149, Length=464, Percent_Identity=29.3103448275862, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24657827, Length=456, Percent_Identity=30.4824561403509, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24585213, Length=466, Percent_Identity=27.8969957081545, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24657842, Length=455, Percent_Identity=28.1318681318681, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24651556, Length=451, Percent_Identity=29.0465631929047, Blast_Score=118, Evalue=8e-27,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 50496; Mature: 50496

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFVKKAWPFAVVASLAVTSVATWNFTRSSEVSADENGSNAKIKNVIVLIGDGMGPSYM
CCHHHHHHCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHH
TAHRYMKDNPKTFEMESTEFDKHLVGTQKTYPEDEHENITDSASAATAMSAGIKTYNAAI
HHHHHHCCCCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEE
AVDNDKAEVKTVLEQAKEKGKSTGLVATSEITHATPAAFGAHDISRKNMDAIANDYFDEK
EECCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHH
IKGKHKVDVMLGGGVKNFVRKDRNLTEEFKKSGYSYVTDRDQLLNDKNDQILGLFGPGGL
HCCCEEEEEEECCCHHHHHHHCCCHHHHHHHCCCCEECCHHHHHCCCCCCEEEEECCCCH
DKMIDRNEKTPSLEEMTNAAIERLNKNKKGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFE
HHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCCHHHHHHHHHHHH
KAFKAAIEFAKKDKNTLVIATADHATGGLSLGANGEYNFKVDPIKAAKRTPDFMANEIAK
HHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCEEEEECCHHHHHCCHHHHHHHHHC
GANVEETLKKYIDLQLTPEEIKAVNDIAPSKDVTKIDNAIEDIFNKRSVTGWTTGGHTGE
CCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCC
DVNVYAFGPGKYLFSGVQENTNLAKRVFDIVGGGDPNKGRR
CEEEEEECCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKFVKKAWPFAVVASLAVTSVATWNFTRSSEVSADENGSNAKIKNVIVLIGDGMGPSYM
CCHHHHHHCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHH
TAHRYMKDNPKTFEMESTEFDKHLVGTQKTYPEDEHENITDSASAATAMSAGIKTYNAAI
HHHHHHCCCCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEE
AVDNDKAEVKTVLEQAKEKGKSTGLVATSEITHATPAAFGAHDISRKNMDAIANDYFDEK
EECCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHH
IKGKHKVDVMLGGGVKNFVRKDRNLTEEFKKSGYSYVTDRDQLLNDKNDQILGLFGPGGL
HCCCEEEEEEECCCHHHHHHHCCCHHHHHHHCCCCEECCHHHHHCCCCCCEEEEECCCCH
DKMIDRNEKTPSLEEMTNAAIERLNKNKKGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFE
HHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCCHHHHHHHHHHHH
KAFKAAIEFAKKDKNTLVIATADHATGGLSLGANGEYNFKVDPIKAAKRTPDFMANEIAK
HHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCEEEEECCHHHHHCCHHHHHHHHHC
GANVEETLKKYIDLQLTPEEIKAVNDIAPSKDVTKIDNAIEDIFNKRSVTGWTTGGHTGE
CCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCC
DVNVYAFGPGKYLFSGVQENTNLAKRVFDIVGGGDPNKGRR
CEEEEEECCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]