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The map label for this gene is aspS [H]

Identifier: 52141106

GI number: 52141106

Start: 4251151

End: 4252926

Strand: Reverse

Name: aspS [H]

Synonym: BCZK4145

Alternate gene names: 52141106

Gene position: 4252926-4251151 (Counterclockwise)

Preceding gene: 52141104

Following gene: 52141105

Centisome position: 80.23

GC content: 40.99

Gene sequence:

>1776_bases
GTGGCTGAAAGAACACATGCATGTGGAAAAGTAACAGTAGAAGCAGTTGGACAAACAGTTCAATTAAAAGGTTGGGTACA
AAAACGCCGTGATTTAGGTGGATTAATCTTTATCGACTTACGTGATCGTACAGGTATCGTACAAGTTGTATTTAACCCAG
AAACATCAAAAGAAGCGCTAGAAGTAGCAGAAACAATTCGTAGCGAATACGTATTACACGTAGAAGGTACAGTTGTTGAG
CGTGGTGAAGGCGCAATTAATGACAATATGGCAACAGGTCGTATTGAAGTACAAGCAACGAAAGTAAACGTATTAAATGC
AGCGAAAACAACGCCAATCATTATTGCTGATGATACAGATGCATCAGAAGATGTACGTTTGAAATATCGTTATTTAGACT
TACGTCGTCCTGTAATGTTTAACACATTCAAAATGCGTCACGACGTAACGAAAACAATTCGTAACTTCTTAGATACAGAA
GAGTTCTTAGAAGTGGAAACACCAATTTTAACGAAGAGCACACCAGAAGGAGCTCGTGACTATTTAGTACCAAGTCGTGT
ACATGACGGTGAATTCTACGCATTACCACAGTCTCCACAGCTATTTAAACAGCTTCTTATGGTCGGTGGATTTGAGCGTT
ACTATCAAGTAGCACGTTGTTTCCGTGACGAAGATTTACGTGCGGATCGTCAACCAGAATTCACGCAAATCGATATCGAG
GCTTCATTCTTAACACAAGATGAAATTTTAGATATGATGGAGCGCATGATGACGAAAGTTATGAAGGATGCAAAAGGTGT
AGAAGTTAGCGCACCATTCCCTCGTATGAAATATGCTGATGCAATGGCTCGATACGGTTCTGATAAGCCAGATACACGCT
TTGAGATGGAACTAACAGACTTATCTGAGTTTGCAGCAGGTTGTGGGTTTAAAGTATTTACAAGTGCTGTAGAAAGCGGC
GGACAAGTAAAAGCAATTAATGCAAAAGGTGCTGCGAGCAAATACTCTCGTAAAGATATCGATGCATTAACTGAATTCGT
AAAAGTATACGGTGCAAAAGGCTTAGCTTGGCTTAAAGTGGAAGAAGACGGCTTAAAAGGACCAATCGCGAAATTCTTCG
GTGAAGAAGATGCGAACGTGTTAATGAATACATTAGAAGCTACTGCTGGTGACTTATTACTATTCGTAGCAGATAAGAAG
AGCGTTGTTGCAGATAGCTTAGGCGCACTTCGTTTACGTCTAGGTAAAGAGCTTGAGTTAATTGACGAAAGTAAATTTAA
CTTCCTATGGGTAACGGATTGGCCACTTCTTGAGTACGATGAAGATGCAGATCGTTACTTCGCAGCTCACCACCCATTCA
CAATGCCATTCCGTGAAGATGTTGAGCTATTAGAAACAGCACCAGAAAAAGCACGTGCACAAGCATATGACCTTGTATTA
AACGGTTATGAGCTTGGTGGTGGATCACTGCGTATTTACGAGCGTGACGTACAAGAAAAAATGTTCAAAGCACTTGGATT
CTCACAAGAAGACGCACAAGAGCAATTCGGATTCTTATTAGAAGCATTCGAATACGGTACGCCACCACACGGTGGTATCG
CATTAGGGTTAGACCGTCTTGTTATGTTACTTGCAGGTCGTACGAACCTTCGTGATACAATTGCATTCCCGAAAACAGCA
AGCGCAAGCTGCTTATTAACAGAAGCTCCAAGCCCAGTTGCAGAAGCACAGCTTGAAGAGTTAAACTTAAAATTAAGCTT
AAAAGAAGAGAAGTAA

Upstream 100 bases:

>100_bases
CATTAACTTAAAAGATATGGCAACAGGCGAACAAGAAGAAGTAGCATTAGATGTGTTTGCTTCATACGTAGCAGAGAAAT
TAATATAGGGGGAACTTACA

Downstream 100 bases:

>100_bases
GAATAAAGTCTAGATGGTTATACTAATACCATCTAGACTTTTTTTGAGAGGAAGTTTAAAAAGTCCGGTTCAGATGGCTG
TCGCATCTCGTCGTTACGTA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MAERTHACGKVTVEAVGQTVQLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFNPETSKEALEVAETIRSEYVLHVEGTVVE
RGEGAINDNMATGRIEVQATKVNVLNAAKTTPIIIADDTDASEDVRLKYRYLDLRRPVMFNTFKMRHDVTKTIRNFLDTE
EFLEVETPILTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMVGGFERYYQVARCFRDEDLRADRQPEFTQIDIE
ASFLTQDEILDMMERMMTKVMKDAKGVEVSAPFPRMKYADAMARYGSDKPDTRFEMELTDLSEFAAGCGFKVFTSAVESG
GQVKAINAKGAASKYSRKDIDALTEFVKVYGAKGLAWLKVEEDGLKGPIAKFFGEEDANVLMNTLEATAGDLLLFVADKK
SVVADSLGALRLRLGKELELIDESKFNFLWVTDWPLLEYDEDADRYFAAHHPFTMPFREDVELLETAPEKARAQAYDLVL
NGYELGGGSLRIYERDVQEKMFKALGFSQEDAQEQFGFLLEAFEYGTPPHGGIALGLDRLVMLLAGRTNLRDTIAFPKTA
SASCLLTEAPSPVAEAQLEELNLKLSLKEEK

Sequences:

>Translated_591_residues
MAERTHACGKVTVEAVGQTVQLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFNPETSKEALEVAETIRSEYVLHVEGTVVE
RGEGAINDNMATGRIEVQATKVNVLNAAKTTPIIIADDTDASEDVRLKYRYLDLRRPVMFNTFKMRHDVTKTIRNFLDTE
EFLEVETPILTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMVGGFERYYQVARCFRDEDLRADRQPEFTQIDIE
ASFLTQDEILDMMERMMTKVMKDAKGVEVSAPFPRMKYADAMARYGSDKPDTRFEMELTDLSEFAAGCGFKVFTSAVESG
GQVKAINAKGAASKYSRKDIDALTEFVKVYGAKGLAWLKVEEDGLKGPIAKFFGEEDANVLMNTLEATAGDLLLFVADKK
SVVADSLGALRLRLGKELELIDESKFNFLWVTDWPLLEYDEDADRYFAAHHPFTMPFREDVELLETAPEKARAQAYDLVL
NGYELGGGSLRIYERDVQEKMFKALGFSQEDAQEQFGFLLEAFEYGTPPHGGIALGLDRLVMLLAGRTNLRDTIAFPKTA
SASCLLTEAPSPVAEAQLEELNLKLSLKEEK
>Mature_590_residues
AERTHACGKVTVEAVGQTVQLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFNPETSKEALEVAETIRSEYVLHVEGTVVER
GEGAINDNMATGRIEVQATKVNVLNAAKTTPIIIADDTDASEDVRLKYRYLDLRRPVMFNTFKMRHDVTKTIRNFLDTEE
FLEVETPILTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMVGGFERYYQVARCFRDEDLRADRQPEFTQIDIEA
SFLTQDEILDMMERMMTKVMKDAKGVEVSAPFPRMKYADAMARYGSDKPDTRFEMELTDLSEFAAGCGFKVFTSAVESGG
QVKAINAKGAASKYSRKDIDALTEFVKVYGAKGLAWLKVEEDGLKGPIAKFFGEEDANVLMNTLEATAGDLLLFVADKKS
VVADSLGALRLRLGKELELIDESKFNFLWVTDWPLLEYDEDADRYFAAHHPFTMPFREDVELLETAPEKARAQAYDLVLN
GYELGGGSLRIYERDVQEKMFKALGFSQEDAQEQFGFLLEAFEYGTPPHGGIALGLDRLVMLLAGRTNLRDTIAFPKTAS
ASCLLTEAPSPVAEAQLEELNLKLSLKEEK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=593, Percent_Identity=41.1467116357504, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI45439306, Length=310, Percent_Identity=25.1612903225806, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI5031815, Length=308, Percent_Identity=25.3246753246753, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI194272210, Length=308, Percent_Identity=25.3246753246753, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI4758762, Length=288, Percent_Identity=26.3888888888889, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788173, Length=594, Percent_Identity=52.3569023569024, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI1789256, Length=307, Percent_Identity=25.0814332247557, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790571, Length=310, Percent_Identity=24.8387096774194, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=613, Percent_Identity=34.094616639478, Blast_Score=335, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI17551876, Length=309, Percent_Identity=26.537216828479, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71994340, Length=190, Percent_Identity=27.3684210526316, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=190, Percent_Identity=27.3684210526316, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=190, Percent_Identity=27.3684210526316, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=634, Percent_Identity=35.0157728706625, Blast_Score=310, Evalue=4e-85,
Organism=Saccharomyces cerevisiae, GI6321807, Length=287, Percent_Identity=27.8745644599303, Blast_Score=90, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6323011, Length=259, Percent_Identity=29.3436293436293, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320242, Length=216, Percent_Identity=27.7777777777778, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324256, Length=136, Percent_Identity=27.2058823529412, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24584738, Length=614, Percent_Identity=39.7394136807818, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17136276, Length=261, Percent_Identity=27.2030651340996, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640851, Length=268, Percent_Identity=26.4925373134328, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640849, Length=268, Percent_Identity=26.4925373134328, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921528, Length=283, Percent_Identity=27.5618374558304, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66321; Mature: 66190

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERTHACGKVTVEAVGQTVQLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFNPETSKEAL
CCCCCCCCCCEEHHHCCCEEEEHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCHHHH
EVAETIRSEYVLHVEGTVVERGEGAINDNMATGRIEVQATKVNVLNAAKTTPIIIADDTD
HHHHHHHHCEEEEEECEEEECCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCEEEECCCC
ASEDVRLKYRYLDLRRPVMFNTFKMRHDVTKTIRNFLDTEEFLEVETPILTKSTPEGARD
CCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCEEECCCCCCCHH
YLVPSRVHDGEFYALPQSPQLFKQLLMVGGFERYYQVARCFRDEDLRADRQPEFTQIDIE
CCCCCEECCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
ASFLTQDEILDMMERMMTKVMKDAKGVEVSAPFPRMKYADAMARYGSDKPDTRFEMELTD
EECCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHCCCCCCCCEEEEEHHH
LSEFAAGCGFKVFTSAVESGGQVKAINAKGAASKYSRKDIDALTEFVKVYGAKGLAWLKV
HHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
EEDGLKGPIAKFFGEEDANVLMNTLEATAGDLLLFVADKKSVVADSLGALRLRLGKELEL
CCCCCCCCHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEE
IDESKFNFLWVTDWPLLEYDEDADRYFAAHHPFTMPFREDVELLETAPEKARAQAYDLVL
ECCCCCCEEEEECCCCCCCCCCCHHEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHEEE
NGYELGGGSLRIYERDVQEKMFKALGFSQEDAQEQFGFLLEAFEYGTPPHGGIALGLDRL
ECEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
VMLLAGRTNLRDTIAFPKTASASCLLTEAPSPVAEAQLEELNLKLSLKEEK
HHHHHCCCCCCHHCCCCCCCCCCEEEECCCCCHHHHHHHHCCEEEEEECCC
>Mature Secondary Structure 
AERTHACGKVTVEAVGQTVQLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFNPETSKEAL
CCCCCCCCCEEHHHCCCEEEEHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCHHHH
EVAETIRSEYVLHVEGTVVERGEGAINDNMATGRIEVQATKVNVLNAAKTTPIIIADDTD
HHHHHHHHCEEEEEECEEEECCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCEEEECCCC
ASEDVRLKYRYLDLRRPVMFNTFKMRHDVTKTIRNFLDTEEFLEVETPILTKSTPEGARD
CCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCEEECCCCCCCHH
YLVPSRVHDGEFYALPQSPQLFKQLLMVGGFERYYQVARCFRDEDLRADRQPEFTQIDIE
CCCCCEECCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
ASFLTQDEILDMMERMMTKVMKDAKGVEVSAPFPRMKYADAMARYGSDKPDTRFEMELTD
EECCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHCCCCCCCCEEEEEHHH
LSEFAAGCGFKVFTSAVESGGQVKAINAKGAASKYSRKDIDALTEFVKVYGAKGLAWLKV
HHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
EEDGLKGPIAKFFGEEDANVLMNTLEATAGDLLLFVADKKSVVADSLGALRLRLGKELEL
CCCCCCCCHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEE
IDESKFNFLWVTDWPLLEYDEDADRYFAAHHPFTMPFREDVELLETAPEKARAQAYDLVL
ECCCCCCEEEEECCCCCCCCCCCHHEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHEEE
NGYELGGGSLRIYERDVQEKMFKALGFSQEDAQEQFGFLLEAFEYGTPPHGGIALGLDRL
ECEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
VMLLAGRTNLRDTIAFPKTASASCLLTEAPSPVAEAQLEELNLKLSLKEEK
HHHHHCCCCCCHHCCCCCCCCCCEEEECCCCCHHHHHHHHCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA