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The map label for this gene is queA [H]

Identifier: 52141088

GI number: 52141088

Start: 4269929

End: 4270981

Strand: Reverse

Name: queA [H]

Synonym: BCZK4162

Alternate gene names: 52141088

Gene position: 4270981-4269929 (Counterclockwise)

Preceding gene: 52141087

Following gene: 52141090

Centisome position: 80.57

GC content: 38.75

Gene sequence:

>1053_bases
ATGGATATTAATCTGTTTGATTTTCATTTACCAGAAGAACTTATTGCACAAGTCCCATTAGAAGAGCGTGAAACATCAAG
ATTAATGGTGTTAGACCGCGAAACAGGGGATATTGAGCATAAACATTTTACGGACATTCTTTCTTACTTACATGAGGGGG
ATTGCTTAGTTTTAAATGAAACGAAAGTAATGCCAGCTCGTTTGCAGGGTGTGAAAGAAGATACAGGCGCACACATTGAA
GTACTTCTTTTAAAACAAGAGGAAGGCGATAAGTGGGAGACGCTTGTTAAGCCAGCGAAACGTGTAAAAGAAGGAACTGT
TATCTCTTTTGGCGAAGGGAAATTAAAAGCAACTTGCACAGGAACTGCAGATCAAGGTGGACGTCAGCTTGAATTTTCAT
ATGACGGCATCTTCTATGAAATTTTAGATGAACTTGGAGAAATGCCACTTCCTCCATATATTAAAGAGACGCTAGAAGAT
CGCGATCGCTATCAAACAGTATATGCGAAAGAAATCGGTTCAGCAGCAGCGCCTACTGCAGGACTTCACTTTACAGAAGA
GTTACTGGAGAAGTTAAAACAAAAAGGCGTTGAGTTAGCATTTATTACACTTCACGTAGGGCTTGGAACATTTAGACCAG
TTTCTGCTGATACGATTGAAGAGCACCATATGCATGCAGAATATTACCATATGTCTGAAGAGACAGCGGCATTATTAAAC
CGTGTAAAAGAGAATGGTGGACGTATTATTACGGTAGGTACGACTTCAACTCGTACGTTAGAAACAATTGCGACAGATCA
TGACGGCAAGCTTTGTGCTGCTTCTGGCTGGACAGACATTTTTATGTACCCAGGTTATGAATTTAAAGCAATTGATGGTT
TAATTACAAACTTCCATTTACCGAAATCAACATTAATTATGCTTGTAAGTGCATTTGCAAACAGAGATAATGTACTTCAT
GCTTATAATGAAGCAGTAAAAGAAAAATATCGTTTCTTTAGCTTCGGTGATGCAATGTTCGTTGCATCTCACGCTAAAAT
GGGAAACAAATAA

Upstream 100 bases:

>100_bases
TTTTATTTTCCTATCATTACATGTATTGTGTTAAGTATTGTATTATCTTTTATTATGTATATAATAAATCGATTCAAATA
AGAAATAGGTGGATATAGTT

Downstream 100 bases:

>100_bases
AAGGAGTAAATTATGACAGCAATTCGTTATGAATTTATTAAAACTTGTAAACAAACAGGTGCTCGTTTAGGACGTGTACA
TACGCCGCACGGTTCATTTG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MDINLFDFHLPEELIAQVPLEERETSRLMVLDRETGDIEHKHFTDILSYLHEGDCLVLNETKVMPARLQGVKEDTGAHIE
VLLLKQEEGDKWETLVKPAKRVKEGTVISFGEGKLKATCTGTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKETLED
RDRYQTVYAKEIGSAAAPTAGLHFTEELLEKLKQKGVELAFITLHVGLGTFRPVSADTIEEHHMHAEYYHMSEETAALLN
RVKENGGRIITVGTTSTRTLETIATDHDGKLCAASGWTDIFMYPGYEFKAIDGLITNFHLPKSTLIMLVSAFANRDNVLH
AYNEAVKEKYRFFSFGDAMFVASHAKMGNK

Sequences:

>Translated_350_residues
MDINLFDFHLPEELIAQVPLEERETSRLMVLDRETGDIEHKHFTDILSYLHEGDCLVLNETKVMPARLQGVKEDTGAHIE
VLLLKQEEGDKWETLVKPAKRVKEGTVISFGEGKLKATCTGTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKETLED
RDRYQTVYAKEIGSAAAPTAGLHFTEELLEKLKQKGVELAFITLHVGLGTFRPVSADTIEEHHMHAEYYHMSEETAALLN
RVKENGGRIITVGTTSTRTLETIATDHDGKLCAASGWTDIFMYPGYEFKAIDGLITNFHLPKSTLIMLVSAFANRDNVLH
AYNEAVKEKYRFFSFGDAMFVASHAKMGNK
>Mature_350_residues
MDINLFDFHLPEELIAQVPLEERETSRLMVLDRETGDIEHKHFTDILSYLHEGDCLVLNETKVMPARLQGVKEDTGAHIE
VLLLKQEEGDKWETLVKPAKRVKEGTVISFGEGKLKATCTGTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKETLED
RDRYQTVYAKEIGSAAAPTAGLHFTEELLEKLKQKGVELAFITLHVGLGTFRPVSADTIEEHHMHAEYYHMSEETAALLN
RVKENGGRIITVGTTSTRTLETIATDHDGKLCAASGWTDIFMYPGYEFKAIDGLITNFHLPKSTLIMLVSAFANRDNVLH
AYNEAVKEKYRFFSFGDAMFVASHAKMGNK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=354, Percent_Identity=47.7401129943503, Blast_Score=317, Evalue=7e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39378; Mature: 39378

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDINLFDFHLPEELIAQVPLEERETSRLMVLDRETGDIEHKHFTDILSYLHEGDCLVLNE
CCCCEEECCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEC
TKVMPARLQGVKEDTGAHIEVLLLKQEEGDKWETLVKPAKRVKEGTVISFGEGKLKATCT
CCCCHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEEEE
GTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKETLEDRDRYQTVYAKEIGSAAAPTA
CCCCCCCCEEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GLHFTEELLEKLKQKGVELAFITLHVGLGTFRPVSADTIEEHHMHAEYYHMSEETAALLN
CCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHCHHHHHCCCHHHHHHHH
RVKENGGRIITVGTTSTRTLETIATDHDGKLCAASGWTDIFMYPGYEFKAIDGLITNFHL
HHHHCCCEEEEEECCCCCEEHEEECCCCCCEEEECCCCEEEEECCCCEEHHHHHHHCCCC
PKSTLIMLVSAFANRDNVLHAYNEAVKEKYRFFSFGDAMFVASHAKMGNK
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHEEEHHHHCCCC
>Mature Secondary Structure
MDINLFDFHLPEELIAQVPLEERETSRLMVLDRETGDIEHKHFTDILSYLHEGDCLVLNE
CCCCEEECCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEC
TKVMPARLQGVKEDTGAHIEVLLLKQEEGDKWETLVKPAKRVKEGTVISFGEGKLKATCT
CCCCHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEEEE
GTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKETLEDRDRYQTVYAKEIGSAAAPTA
CCCCCCCCEEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GLHFTEELLEKLKQKGVELAFITLHVGLGTFRPVSADTIEEHHMHAEYYHMSEETAALLN
CCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHCHHHHHCCCHHHHHHHH
RVKENGGRIITVGTTSTRTLETIATDHDGKLCAASGWTDIFMYPGYEFKAIDGLITNFHL
HHHHCCCEEEEEECCCCCEEHEEECCCCCCEEEECCCCEEEEECCCCEEHHHHHHHCCCC
PKSTLIMLVSAFANRDNVLHAYNEAVKEKYRFFSFGDAMFVASHAKMGNK
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA