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The map label for this gene is folC [H]

Identifier: 52141049

GI number: 52141049

Start: 4306553

End: 4307854

Strand: Reverse

Name: folC [H]

Synonym: BCZK4201

Alternate gene names: 52141049

Gene position: 4307854-4306553 (Counterclockwise)

Preceding gene: 52141048

Following gene: 52141050

Centisome position: 81.27

GC content: 37.02

Gene sequence:

>1302_bases
GTGATACATACATACGAAGAAGCGATAGGGTGGATTCATAGCCGATTGAAATTTGGTATTAAACCGGGATTAGAAAGAAT
GAGATGGATGCTAGAAGAGCTCGGAAATCCAGAGCGCCATATAAAATGTGTTCATCTTGCTGGTACAAATGGAAAAGGTT
CAACGTTAACATATATGCGCTACATGTTAGAAGGTGCAAAATATAAGGTAGGAACATTTACCTCCCCTTATATTGAAACA
TTTAACGAGCGTATTAGTGTGAACGGAACACCAATTGCAGATGAAGAGATTACTGAACTTGTAAAGATGGTAAAGCCAGT
CGTTGAAAAACTAGATGAGACGGATTTAGGGGAAGCGACTGAGTTTGAAATTATTACTGTTATGGCAATTTGTTATTTCG
GTAAAGTTAATTTCTGTGATGTTGTTTTATTTGAAACAGGGCTTGGAGGGCGCTTTGATTCTACGAATGTGATTCATCCA
GTTCTCACGATTATTACGAATATCGGTCACGATCATATGCATATTTTAGGTAATACACTAGAAGAAATTGCATATGAAAA
GGCGGGGATTATTAAATCTGGTGTTCCGGTTATTACTGGTGTGCAAGATGAGGAAGCGTTGCAAGTCATTCAAAAAATTG
CAAAGGAAAATCAGGCAAACTTATACGAGATGGGCAACCATTTTACATCGTTACATAAACAGTCTAGTGAAGATGGAGAA
CAGTTCGATTTCACTTGTCCTTTCGCCTCTTTTGAAGATGTGCGAATCACAATGAAAGGCAGCCATCAAGTAGGAAACGC
AGCGCTTGCGCTTATGGCAGTCATGTATGTAAAAACATACTTATCATTTTTAATTGAGGAAGAAGAAATAAGAACTGGAT
TACAGGAAGCGTATTGGGTGGGGCGCTTTGAAAAACTGCAAAGTAATCCGGATATTATTATAGATGGTGCTCATAACCCA
GAAGGTATTGAGAGTCTTGTGAAAACGGTAGAATCACATTACAAAGATAAAAATGTAATAGTTTTATTTACTGCTCTTGG
TGATAAACAATTGCACAATATGGTAGATCAATTAGAAACCATCGCCGATGAAATTATTTTTACAACATTCGCCTTTGATC
GTGCTATTTCAGCAGGCGAGCTTGCGTCGTATGCAAAGAAAGAGTCAAAATTAGTTTTTGAAAATTGGAAAGAGGCAATT
GATACGAAGGTTGAAATGATTGGAGAAAATGATGTTTTTATCATAACAGGTTCTCTTTATTTCATTTCGGAAGTTCGAAA
ATATATTCGCGAGAAAAACTAG

Upstream 100 bases:

>100_bases
GAAATAAAAATGTTTTTTAGAGACTCACTATATTGGTGAGTCTCTTTTCTTTGCGTATAATAGAAAGATGAAGGTTGTCT
TTTGAAAGGGGAAAGCATAC

Downstream 100 bases:

>100_bases
ATAGTCTTTGCTATCTAGTTTTTTTGTTCTACAAATGAAGTCAAATGCATACATATAAGATAAATATATAGGGTTATTTG
GCGAATTCCTCTGACAAATA

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MIHTYEEAIGWIHSRLKFGIKPGLERMRWMLEELGNPERHIKCVHLAGTNGKGSTLTYMRYMLEGAKYKVGTFTSPYIET
FNERISVNGTPIADEEITELVKMVKPVVEKLDETDLGEATEFEIITVMAICYFGKVNFCDVVLFETGLGGRFDSTNVIHP
VLTIITNIGHDHMHILGNTLEEIAYEKAGIIKSGVPVITGVQDEEALQVIQKIAKENQANLYEMGNHFTSLHKQSSEDGE
QFDFTCPFASFEDVRITMKGSHQVGNAALALMAVMYVKTYLSFLIEEEEIRTGLQEAYWVGRFEKLQSNPDIIIDGAHNP
EGIESLVKTVESHYKDKNVIVLFTALGDKQLHNMVDQLETIADEIIFTTFAFDRAISAGELASYAKKESKLVFENWKEAI
DTKVEMIGENDVFIITGSLYFISEVRKYIREKN

Sequences:

>Translated_433_residues
MIHTYEEAIGWIHSRLKFGIKPGLERMRWMLEELGNPERHIKCVHLAGTNGKGSTLTYMRYMLEGAKYKVGTFTSPYIET
FNERISVNGTPIADEEITELVKMVKPVVEKLDETDLGEATEFEIITVMAICYFGKVNFCDVVLFETGLGGRFDSTNVIHP
VLTIITNIGHDHMHILGNTLEEIAYEKAGIIKSGVPVITGVQDEEALQVIQKIAKENQANLYEMGNHFTSLHKQSSEDGE
QFDFTCPFASFEDVRITMKGSHQVGNAALALMAVMYVKTYLSFLIEEEEIRTGLQEAYWVGRFEKLQSNPDIIIDGAHNP
EGIESLVKTVESHYKDKNVIVLFTALGDKQLHNMVDQLETIADEIIFTTFAFDRAISAGELASYAKKESKLVFENWKEAI
DTKVEMIGENDVFIITGSLYFISEVRKYIREKN
>Mature_433_residues
MIHTYEEAIGWIHSRLKFGIKPGLERMRWMLEELGNPERHIKCVHLAGTNGKGSTLTYMRYMLEGAKYKVGTFTSPYIET
FNERISVNGTPIADEEITELVKMVKPVVEKLDETDLGEATEFEIITVMAICYFGKVNFCDVVLFETGLGGRFDSTNVIHP
VLTIITNIGHDHMHILGNTLEEIAYEKAGIIKSGVPVITGVQDEEALQVIQKIAKENQANLYEMGNHFTSLHKQSSEDGE
QFDFTCPFASFEDVRITMKGSHQVGNAALALMAVMYVKTYLSFLIEEEEIRTGLQEAYWVGRFEKLQSNPDIIIDGAHNP
EGIESLVKTVESHYKDKNVIVLFTALGDKQLHNMVDQLETIADEIIFTTFAFDRAISAGELASYAKKESKLVFENWKEAI
DTKVEMIGENDVFIITGSLYFISEVRKYIREKN

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=362, Percent_Identity=27.6243093922652, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI66932990, Length=362, Percent_Identity=27.6243093922652, Blast_Score=139, Evalue=7e-33,
Organism=Escherichia coli, GI1788654, Length=432, Percent_Identity=27.5462962962963, Blast_Score=144, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17553150, Length=412, Percent_Identity=25.2427184466019, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71984923, Length=412, Percent_Identity=25.2427184466019, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17553148, Length=412, Percent_Identity=25.2427184466019, Blast_Score=135, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6323760, Length=434, Percent_Identity=29.2626728110599, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324815, Length=369, Percent_Identity=28.7262872628726, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322718, Length=365, Percent_Identity=21.9178082191781, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24641571, Length=335, Percent_Identity=30.1492537313433, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24581568, Length=184, Percent_Identity=31.5217391304348, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 48952; Mature: 48952

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHTYEEAIGWIHSRLKFGIKPGLERMRWMLEELGNPERHIKCVHLAGTNGKGSTLTYMR
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHH
YMLEGAKYKVGTFTSPYIETFNERISVNGTPIADEEITELVKMVKPVVEKLDETDLGEAT
HHHCCCCEEECCCCCHHHHHHHCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
EFEIITVMAICYFGKVNFCDVVLFETGLGGRFDSTNVIHPVLTIITNIGHDHMHILGNTL
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
EEIAYEKAGIIKSGVPVITGVQDEEALQVIQKIAKENQANLYEMGNHFTSLHKQSSEDGE
HHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
QFDFTCPFASFEDVRITMKGSHQVGNAALALMAVMYVKTYLSFLIEEEEIRTGLQEAYWV
CEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRFEKLQSNPDIIIDGAHNPEGIESLVKTVESHYKDKNVIVLFTALGDKQLHNMVDQLET
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHH
IADEIIFTTFAFDRAISAGELASYAKKESKLVFENWKEAIDTKVEMIGENDVFIITGSLY
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHH
FISEVRKYIREKN
HHHHHHHHHHCCC
>Mature Secondary Structure
MIHTYEEAIGWIHSRLKFGIKPGLERMRWMLEELGNPERHIKCVHLAGTNGKGSTLTYMR
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHH
YMLEGAKYKVGTFTSPYIETFNERISVNGTPIADEEITELVKMVKPVVEKLDETDLGEAT
HHHCCCCEEECCCCCHHHHHHHCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
EFEIITVMAICYFGKVNFCDVVLFETGLGGRFDSTNVIHPVLTIITNIGHDHMHILGNTL
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
EEIAYEKAGIIKSGVPVITGVQDEEALQVIQKIAKENQANLYEMGNHFTSLHKQSSEDGE
HHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
QFDFTCPFASFEDVRITMKGSHQVGNAALALMAVMYVKTYLSFLIEEEEIRTGLQEAYWV
CEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRFEKLQSNPDIIIDGAHNPEGIESLVKTVESHYKDKNVIVLFTALGDKQLHNMVDQLET
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHH
IADEIIFTTFAFDRAISAGELASYAKKESKLVFENWKEAIDTKVEMIGENDVFIITGSLY
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHH
FISEVRKYIREKN
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]