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The map label for this gene is tnpR [H]

Identifier: 52141027

GI number: 52141027

Start: 4338165

End: 4338728

Strand: Direct

Name: tnpR [H]

Synonym: BCZK4228

Alternate gene names: 52141027

Gene position: 4338165-4338728 (Clockwise)

Preceding gene: 52141030

Following gene: 52141006

Centisome position: 81.84

GC content: 41.67

Gene sequence:

>564_bases
ATGACAGTATTCGGATATGCTCGTGTATCTTCTAAGGGACAAGACCTGTCAGTACAGATTGAAGAGCTTACAAACAAAGG
AGCAACTGAGATTTACTCAGAGAAATTTACAGGAGCTACCTCTGACCGCCCTGAGCTTCAGAGAGTACTAAATGTATTGA
AAGAAGGAGATACACTTATAGTAACCAAGCTTGACCGTCTGGCAAGGAATACAAAAGAGGGAATTCAAATTGTAGAGGAG
CTATTCTCGAAAGGTGTCAGCGTTCATGTACTGAATGTTGGGCTTCTTGAAAATACTACGATGGGAAGATTTTTCTTACA
GACAATGCTTGCGGTTGCGGAGATGGAGCGATCTATGATTTATGAGAGAACTCAAGAAGGCAAGGAACATGCTAAGGCAA
ACAATCCAAACTATAAAGAAGGACGTCCGAAAGCTCTTACTACTAGCCGTTACGCTGAGTGCATGAAGCTTTTAGAGACT
AATTCCGTTAAGCAAGTAGCAAACATTATGAATATATCCGAGAGAACAATCTATAGGTACAAGCGTGAGGGTCAGGCGGA
ATAG

Upstream 100 bases:

>100_bases
ACTATGACAAAACTCAACAGATTTGACAGTATGATTTCAGAATGATAGAATCAACCTTATTAACATGACAAAACTTACTG
ACAAAAGGAGAGGTGACAAA

Downstream 100 bases:

>100_bases
TCGGACTCTCTTTGTGCATTTTTTTGCTTATAAGAGCCTTAATATAAGAGTATTTATGTCTCAGACACTGGTAGCCACTA
AAGGGGAATTTCATCAGATT

Product: resolvase family site-specific recombinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MTVFGYARVSSKGQDLSVQIEELTNKGATEIYSEKFTGATSDRPELQRVLNVLKEGDTLIVTKLDRLARNTKEGIQIVEE
LFSKGVSVHVLNVGLLENTTMGRFFLQTMLAVAEMERSMIYERTQEGKEHAKANNPNYKEGRPKALTTSRYAECMKLLET
NSVKQVANIMNISERTIYRYKREGQAE

Sequences:

>Translated_187_residues
MTVFGYARVSSKGQDLSVQIEELTNKGATEIYSEKFTGATSDRPELQRVLNVLKEGDTLIVTKLDRLARNTKEGIQIVEE
LFSKGVSVHVLNVGLLENTTMGRFFLQTMLAVAEMERSMIYERTQEGKEHAKANNPNYKEGRPKALTTSRYAECMKLLET
NSVKQVANIMNISERTIYRYKREGQAE
>Mature_186_residues
TVFGYARVSSKGQDLSVQIEELTNKGATEIYSEKFTGATSDRPELQRVLNVLKEGDTLIVTKLDRLARNTKEGIQIVEEL
FSKGVSVHVLNVGLLENTTMGRFFLQTMLAVAEMERSMIYERTQEGKEHAKANNPNYKEGRPKALTTSRYAECMKLLETN
SVKQVANIMNISERTIYRYKREGQAE

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=178, Percent_Identity=38.2022471910112, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787827, Length=187, Percent_Identity=34.7593582887701, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787638, Length=187, Percent_Identity=34.7593582887701, Blast_Score=95, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 21204; Mature: 21072

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVFGYARVSSKGQDLSVQIEELTNKGATEIYSEKFTGATSDRPELQRVLNVLKEGDTLI
CCEECCCCCCCCCCCCEEEHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEE
VTKLDRLARNTKEGIQIVEELFSKGVSVHVLNVGLLENTTMGRFFLQTMLAVAEMERSMI
EHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHH
YERTQEGKEHAKANNPNYKEGRPKALTTSRYAECMKLLETNSVKQVANIMNISERTIYRY
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KREGQAE
HHCCCCC
>Mature Secondary Structure 
TVFGYARVSSKGQDLSVQIEELTNKGATEIYSEKFTGATSDRPELQRVLNVLKEGDTLI
CEECCCCCCCCCCCCEEEHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEE
VTKLDRLARNTKEGIQIVEELFSKGVSVHVLNVGLLENTTMGRFFLQTMLAVAEMERSMI
EHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHH
YERTQEGKEHAKANNPNYKEGRPKALTTSRYAECMKLLETNSVKQVANIMNISERTIYRY
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KREGQAE
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2997130 [H]