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The map label for this gene is murI [H]

Identifier: 52141014

GI number: 52141014

Start: 4346850

End: 4347659

Strand: Reverse

Name: murI [H]

Synonym: BCZK4236

Alternate gene names: 52141014

Gene position: 4347659-4346850 (Counterclockwise)

Preceding gene: 52141011

Following gene: 52141015

Centisome position: 82.02

GC content: 38.02

Gene sequence:

>810_bases
ATGAAGTTGAATAGAGCAATCGGTGTTATCGATTCAGGAGTTGGCGGCTTAACAGTAGCGAAGGAATTAATTCGTCAGTT
GCCGAAAGAGCGCATTATATATTTAGGGGATACAGAACGTTGCCCTTATGGTCCACGTTCTCGAGAAGAAGTGCGCCAAT
TCACGTGGGAAATGACGGAGCATTTACTAGATTTAAATATCAAAATGTTAGTTATTGCATGCAATACAGCAACTGCAGTT
GTATTAGAAGAGATGCAGAAACAATTACCAATTCCAGTGGTAGGAGTTATTCACCCAGGATCACGTACAGCTTTAAAAAT
GACAAACACGTATCATGTTGGGATTATTGGAACGATCGGAACGGTGAAAAGTGGTGCATATGAAGAGGCGTTAAAGTCTA
TTAATAACCGTGTTGTGGTAGAAAGTTTAGCGTGTCCGCCTTTCGTTGAGCTTGTAGAGAGTGGCAATTTTGAAAGTGAA
ATGGCATATGAAGTTGTAAGAGAAACGTTGCAACCTCTGAAAAACACTGATATTGATACGCTTATTTTAGGTTGTACACA
TTATCCGATTTTAGGCCCTGTTATTAAACAAGTAATGGGAGATAAAGTGCAACTCATTAGTTCTGGTGATGAAACAGCGC
GTGAAGTAAGTACAATTTTATACCATAGTAAAATGTTGAATGAGGGAGAGGAACAAAGCGACCATCTCTTCTTAACAACA
GGGAAAATAGGCTTATTTAAAGAAATTGCATCAAAATGGTTCGGTCAACCAATTGAAAATGTGAAACATATTCATTTAGA
AAAAGAATAA

Upstream 100 bases:

>100_bases
GGTACAAATGAAAATTTTCATACAGAAGCAGTTTAAGTGCGCGAACTTAAAGTGTGTAAAATATAATGATAAAATAAGAT
GATGAATGAGAAAACGGGTG

Downstream 100 bases:

>100_bases
TAGTAGAATCCATATAAGTGAACTCCTGAGAAATCAGGGGTTTTTTGTTTATAGGGAGCACAGCTTGTTCTAAAAAGAGA
AACAGCTCGTATACATAATA

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKLNRAIGVIDSGVGGLTVAKELIRQLPKERIIYLGDTERCPYGPRSREEVRQFTWEMTEHLLDLNIKMLVIACNTATAV
VLEEMQKQLPIPVVGVIHPGSRTALKMTNTYHVGIIGTIGTVKSGAYEEALKSINNRVVVESLACPPFVELVESGNFESE
MAYEVVRETLQPLKNTDIDTLILGCTHYPILGPVIKQVMGDKVQLISSGDETAREVSTILYHSKMLNEGEEQSDHLFLTT
GKIGLFKEIASKWFGQPIENVKHIHLEKE

Sequences:

>Translated_269_residues
MKLNRAIGVIDSGVGGLTVAKELIRQLPKERIIYLGDTERCPYGPRSREEVRQFTWEMTEHLLDLNIKMLVIACNTATAV
VLEEMQKQLPIPVVGVIHPGSRTALKMTNTYHVGIIGTIGTVKSGAYEEALKSINNRVVVESLACPPFVELVESGNFESE
MAYEVVRETLQPLKNTDIDTLILGCTHYPILGPVIKQVMGDKVQLISSGDETAREVSTILYHSKMLNEGEEQSDHLFLTT
GKIGLFKEIASKWFGQPIENVKHIHLEKE
>Mature_269_residues
MKLNRAIGVIDSGVGGLTVAKELIRQLPKERIIYLGDTERCPYGPRSREEVRQFTWEMTEHLLDLNIKMLVIACNTATAV
VLEEMQKQLPIPVVGVIHPGSRTALKMTNTYHVGIIGTIGTVKSGAYEEALKSINNRVVVESLACPPFVELVESGNFESE
MAYEVVRETLQPLKNTDIDTLILGCTHYPILGPVIKQVMGDKVQLISSGDETAREVSTILYHSKMLNEGEEQSDHLFLTT
GKIGLFKEIASKWFGQPIENVKHIHLEKE

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=220, Percent_Identity=33.1818181818182, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 29967; Mature: 29967

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00923 ASP_GLU_RACEMASE_1 ; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNRAIGVIDSGVGGLTVAKELIRQLPKERIIYLGDTERCPYGPRSREEVRQFTWEMTE
CCCCHHHHHHCCCCCHHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHH
HLLDLNIKMLVIACNTATAVVLEEMQKQLPIPVVGVIHPGSRTALKMTNTYHVGIIGTIG
HHHCCCEEEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCEEEEEEEECCC
TVKSGAYEEALKSINNRVVVESLACPPFVELVESGNFESEMAYEVVRETLQPLKNTDIDT
HHCCCHHHHHHHHHCCEEEEHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHH
LILGCTHYPILGPVIKQVMGDKVQLISSGDETAREVSTILYHSKMLNEGEEQSDHLFLTT
EEECCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
GKIGLFKEIASKWFGQPIENVKHIHLEKE
CCHHHHHHHHHHHCCCCHHHHHHEEECCC
>Mature Secondary Structure
MKLNRAIGVIDSGVGGLTVAKELIRQLPKERIIYLGDTERCPYGPRSREEVRQFTWEMTE
CCCCHHHHHHCCCCCHHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHH
HLLDLNIKMLVIACNTATAVVLEEMQKQLPIPVVGVIHPGSRTALKMTNTYHVGIIGTIG
HHHCCCEEEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCEEEEEEEECCC
TVKSGAYEEALKSINNRVVVESLACPPFVELVESGNFESEMAYEVVRETLQPLKNTDIDT
HHCCCHHHHHHHHHCCEEEEHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHH
LILGCTHYPILGPVIKQVMGDKVQLISSGDETAREVSTILYHSKMLNEGEEQSDHLFLTT
EEECCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
GKIGLFKEIASKWFGQPIENVKHIHLEKE
CCHHHHHHHHHHHCCCCHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9311114 [H]