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The map label for this gene is dnaB [H]

Identifier: 52140929

GI number: 52140929

Start: 4434166

End: 4435572

Strand: Reverse

Name: dnaB [H]

Synonym: BCZK4321

Alternate gene names: 52140929

Gene position: 4435572-4434166 (Counterclockwise)

Preceding gene: 52140927

Following gene: 52140930

Centisome position: 83.68

GC content: 38.66

Gene sequence:

>1407_bases
ATGGAAAAACAGTCATGGATGGAGCTATTGCCGATTGATCGTTATAAAGTAAGTGCGAAAGGGTTATTGCATAATTACGA
CCGAAAAGTGTTAACGATGTTATATCAGCCGTTAATAGGTAGTAGAGCTTTTAGCTTGTACATGACGCTATGGGGAGAGT
TAGAGCAAGATCGTGTATTTGGAAAAGAGAATACGCATCATTCCCTTATGGTGACCATGCAAATGCAGCTTCCCGAAGTA
TATGAGGAACGAGTAAAGTTAGAGGCAATTGGACTTTTGAAAGTATATATTAAGAAAGAAAAAGATATTCGAATGTTTAT
ATATGAGCTGCAACCACCTTTATCTCCAAAGCAGTTTTTTGATGATATTGTTTTAAGTATCTTTTTATACAATCGATTGA
GTCGGACAAAATATAATCAAGTAAAGCATTATTTCTTAGAAGAAGAATTTGATTTTGCATCTTATGAAAATGTTACGCGT
TCTTTTAACGATGTGTTTGGTTCGTTTAATCCAGGGCAGCTTGAGCATGCGCAAGAAGACCTTCGTATTCCAAAAACGAC
AGCTATGCCAAGTAATGAAAAGGGAAACGCTCCAAAAGTTTGGAATGATTTCTTTGATTTCTCCTTATTTGTAGACGGCT
TGTCCGCTCTTGTCCCTAAAAAGGCGATTACAGATCAAGTGCGAGAATGTGTCATTACACTTGCTTACGTGTATGATGTA
GATGTGTTATCGATGCAAAATATCGTTCTTGGTGCGGTGACAGAGATGCAGACAATTGATATGGAAAGGCTTAGAAAAGG
AGCGCGTGATTGGTATCAGTTCGAAAACGGTCAAGCATTACCTGTATTAAGTGAAAGAGTACAGCCTCACGCTGCACGTA
TGATGAAAGAAAAAGAGCCTTCTACGCAAGAAGAGATGTTAATCAAACAGCTAGAGGAAATCTCACCAAAGCAACTATTG
AAAGAAATCTCTGGTGGTGCAGAGGCGACGAAGGCTGACTTGCAAATTGTTGAAGATGTAATGATCAATCAAAAATTAAC
GCCAGGTGTTGTGAATGTACTTATTTATTACGTTATGCTACGCTCAGATATGAAACTTGCGAAAACGTATGTGGAGAAGA
TTGCTGGACATTGGGCACGTAAAAAGGTCGGTACAGTAGCTGAAGCAATGGCGTTAGCGAAAGAAGAAAACCGTCAATAT
CAAGAATGGGCTGAGACGAAGAAGAAAGGCCGTACATCGAAGAAAACGGTACGAAAAGAAATGGTACCAGATTGGCTGAA
AGAAGAGCCGAAAGAAAAAGAGAAAGAAACTGTGAAAAAAGATGCAAGTGCTGAAAAAGGTGCAAGTACGTTAGAGGATG
AACGGAAACGACTAGAAGAAGTGTTAAAAAAATATAAGCGTGATTAA

Upstream 100 bases:

>100_bases
CGTAAGTAATGAGATAAGAGAGCTAGAAGCGAAAGCTAATAGCTCTTTTTCGCTAAGAAATTATATAGTGAAGATTGGAT
GAGAGAAAGGAAAAGCAAGA

Downstream 100 bases:

>100_bases
TAGAAGAGAATGAACGCTCTTTGAGGTGAGGAAATGGAGCATATTCAAAATTCATTTGCAAAATTAATGGAAAATGAAAA
TTTTAAAAATAGATATGAAG

Product: DNA replication protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MEKQSWMELLPIDRYKVSAKGLLHNYDRKVLTMLYQPLIGSRAFSLYMTLWGELEQDRVFGKENTHHSLMVTMQMQLPEV
YEERVKLEAIGLLKVYIKKEKDIRMFIYELQPPLSPKQFFDDIVLSIFLYNRLSRTKYNQVKHYFLEEEFDFASYENVTR
SFNDVFGSFNPGQLEHAQEDLRIPKTTAMPSNEKGNAPKVWNDFFDFSLFVDGLSALVPKKAITDQVRECVITLAYVYDV
DVLSMQNIVLGAVTEMQTIDMERLRKGARDWYQFENGQALPVLSERVQPHAARMMKEKEPSTQEEMLIKQLEEISPKQLL
KEISGGAEATKADLQIVEDVMINQKLTPGVVNVLIYYVMLRSDMKLAKTYVEKIAGHWARKKVGTVAEAMALAKEENRQY
QEWAETKKKGRTSKKTVRKEMVPDWLKEEPKEKEKETVKKDASAEKGASTLEDERKRLEEVLKKYKRD

Sequences:

>Translated_468_residues
MEKQSWMELLPIDRYKVSAKGLLHNYDRKVLTMLYQPLIGSRAFSLYMTLWGELEQDRVFGKENTHHSLMVTMQMQLPEV
YEERVKLEAIGLLKVYIKKEKDIRMFIYELQPPLSPKQFFDDIVLSIFLYNRLSRTKYNQVKHYFLEEEFDFASYENVTR
SFNDVFGSFNPGQLEHAQEDLRIPKTTAMPSNEKGNAPKVWNDFFDFSLFVDGLSALVPKKAITDQVRECVITLAYVYDV
DVLSMQNIVLGAVTEMQTIDMERLRKGARDWYQFENGQALPVLSERVQPHAARMMKEKEPSTQEEMLIKQLEEISPKQLL
KEISGGAEATKADLQIVEDVMINQKLTPGVVNVLIYYVMLRSDMKLAKTYVEKIAGHWARKKVGTVAEAMALAKEENRQY
QEWAETKKKGRTSKKTVRKEMVPDWLKEEPKEKEKETVKKDASAEKGASTLEDERKRLEEVLKKYKRD
>Mature_468_residues
MEKQSWMELLPIDRYKVSAKGLLHNYDRKVLTMLYQPLIGSRAFSLYMTLWGELEQDRVFGKENTHHSLMVTMQMQLPEV
YEERVKLEAIGLLKVYIKKEKDIRMFIYELQPPLSPKQFFDDIVLSIFLYNRLSRTKYNQVKHYFLEEEFDFASYENVTR
SFNDVFGSFNPGQLEHAQEDLRIPKTTAMPSNEKGNAPKVWNDFFDFSLFVDGLSALVPKKAITDQVRECVITLAYVYDV
DVLSMQNIVLGAVTEMQTIDMERLRKGARDWYQFENGQALPVLSERVQPHAARMMKEKEPSTQEEMLIKQLEEISPKQLL
KEISGGAEATKADLQIVEDVMINQKLTPGVVNVLIYYVMLRSDMKLAKTYVEKIAGHWARKKVGTVAEAMALAKEENRQY
QEWAETKKKGRTSKKTVRKEMVPDWLKEEPKEKEKETVKKDASAEKGASTLEDERKRLEEVLKKYKRD

Specific function: Probable component of primosome involved in the initiation of DNA replication. It is essential for both replication initiation and membrane attachment of the origin region of the chromosome and plasmid pUB110 [H]

COG id: COG3611

COG function: function code L; Replication initiation/membrane attachment protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006343 [H]

Pfam domain/function: PF07261 DnaB_2 [H]

EC number: NA

Molecular weight: Translated: 54546; Mature: 54546

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKQSWMELLPIDRYKVSAKGLLHNYDRKVLTMLYQPLIGSRAFSLYMTLWGELEQDRVF
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GKENTHHSLMVTMQMQLPEVYEERVKLEAIGLLKVYIKKEKDIRMFIYELQPPLSPKQFF
CCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHH
DDIVLSIFLYNRLSRTKYNQVKHYFLEEEFDFASYENVTRSFNDVFGSFNPGQLEHAQED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LRIPKTTAMPSNEKGNAPKVWNDFFDFSLFVDGLSALVPKKAITDQVRECVITLAYVYDV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
DVLSMQNIVLGAVTEMQTIDMERLRKGARDWYQFENGQALPVLSERVQPHAARMMKEKEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHCCC
STQEEMLIKQLEEISPKQLLKEISGGAEATKADLQIVEDVMINQKLTPGVVNVLIYYVML
CHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RSDMKLAKTYVEKIAGHWARKKVGTVAEAMALAKEENRQYQEWAETKKKGRTSKKTVRKE
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
MVPDWLKEEPKEKEKETVKKDASAEKGASTLEDERKRLEEVLKKYKRD
HCHHHHHCCCHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKQSWMELLPIDRYKVSAKGLLHNYDRKVLTMLYQPLIGSRAFSLYMTLWGELEQDRVF
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GKENTHHSLMVTMQMQLPEVYEERVKLEAIGLLKVYIKKEKDIRMFIYELQPPLSPKQFF
CCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHH
DDIVLSIFLYNRLSRTKYNQVKHYFLEEEFDFASYENVTRSFNDVFGSFNPGQLEHAQED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LRIPKTTAMPSNEKGNAPKVWNDFFDFSLFVDGLSALVPKKAITDQVRECVITLAYVYDV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
DVLSMQNIVLGAVTEMQTIDMERLRKGARDWYQFENGQALPVLSERVQPHAARMMKEKEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHCCC
STQEEMLIKQLEEISPKQLLKEISGGAEATKADLQIVEDVMINQKLTPGVVNVLIYYVML
CHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RSDMKLAKTYVEKIAGHWARKKVGTVAEAMALAKEENRQYQEWAETKKKGRTSKKTVRKE
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
MVPDWLKEEPKEKEKETVKKDASAEKGASTLEDERKRLEEVLKKYKRD
HCHHHHHCCCHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3027697; 3027671; 8969504; 9387221; 9384377 [H]