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The map label for this gene is ackA [H]

Identifier: 52140870

GI number: 52140870

Start: 4489139

End: 4490332

Strand: Reverse

Name: ackA [H]

Synonym: BCZK4381

Alternate gene names: 52140870

Gene position: 4490332-4489139 (Counterclockwise)

Preceding gene: 52140869

Following gene: 52140871

Centisome position: 84.71

GC content: 40.62

Gene sequence:

>1194_bases
ATGTCAAAAATCATCGCGATTAACGCAGGAAGCTCTTCCTTAAAATTCCAATTATTTGAAATGCCAAGTGAAACAGTATT
AACAAAAGGTTTAGTAGAACGTATCGGTTTAGAAGATAGTATCTTCACTATTACTGTAGATGGCGAAAAACAAAAAGAAA
TTACAAACATTCCAGATCACGCAGTAGCAGTTAATATGCTTCTTAAAAAATTAACTGAAAACGGAATCGTTAAATCTCTA
GATGAGATTGGCGGTATCGGTCACCGTGTTGTTCACGGCGGCGAAAAATTTGCTGACTCTGTTCTAATTACTGATGAAGT
ATTAGCTGATATCGAAGAATTAAGCGATTTAGCACCACTTCACAACCCAGCAAACGTTGTTGGTATTAAAGCATTCCAAG
AAGTATTACCAAACGTACCAGCAGTAGCAGTATTCGATACAGCATTCCACCAAACAATGCCGGAATCTGCATTCCTATAC
AGCTTACCGTATGAGTACTACGAAAAGTTCGGTATCCGTAAGTACGGTTTCCACGGAACTTCTCATAAATATGTAACTGA
GCGTGCGGCTGAACTATTAGGCCGTCCACTTGAGAGCTTAAGCTTACTTTCTTGTCACTTAGGTAACGGTGCAAGTATCG
CAGCAGTAGAAGGCGGTAAATCTATCGATACTTCTATGGGCTTCACTCCACTTGCTGGTGTAACAATGGGTACACGTTCT
GGTAACATTGACCCTGCGTTAATTCCATACATCATGGAAAAAACAGGCCAAACAGTAGAAGAAGTAGTTAACGTGCTAAA
CAAGAAAAGTGGTATGTTAGGTCTTACTGGTTACTCTAGTGACCTACGTGACATCATTGCGAAAGAAGAAGAAGGCGATC
ACCGTGCGAAAGTAGCACTTGATGTATTCGTAAGCCGTATCCACAAATACATCGGTTCTTACACTGCTCGTATGAAAGGT
GTAGACGCAATCATCTTCACAGCTGGTGTAGGTGAAAACAGTGCGATTATTCGTGAACGCGTATTAGAAGGCCTTGAATA
CATGGGCGTATACTTCGATGCAAAACGCAATAACGTATTCGGTGAGGAAGCATTCATCAACTTCCCACATTCTCCAGTAA
AAATTATTGTAATTCCAACTGACGAAGAAGTTATGATCGCTCGTGACGTACTACGTCTTGGAAACATCGGTTAA

Upstream 100 bases:

>100_bases
GATGAAACGTTTTCATATTTAGTAAATTTGATCAGATAAAATCGAGCGGTTTGTGGAAACATCCACACAACTACCAAGAG
AAAAAGGGGCGAAAGACTAG

Downstream 100 bases:

>100_bases
TATATGAAAAGAAAGACGAAGTCCTATAGGATTTCGTCTTTTTTATTTTGGATATATGTTAAAATTCCATCTGGATATAG
TACTTTTTTCTCCTGAATGA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSKIIAINAGSSSLKFQLFEMPSETVLTKGLVERIGLEDSIFTITVDGEKQKEITNIPDHAVAVNMLLKKLTENGIVKSL
DEIGGIGHRVVHGGEKFADSVLITDEVLADIEELSDLAPLHNPANVVGIKAFQEVLPNVPAVAVFDTAFHQTMPESAFLY
SLPYEYYEKFGIRKYGFHGTSHKYVTERAAELLGRPLESLSLLSCHLGNGASIAAVEGGKSIDTSMGFTPLAGVTMGTRS
GNIDPALIPYIMEKTGQTVEEVVNVLNKKSGMLGLTGYSSDLRDIIAKEEEGDHRAKVALDVFVSRIHKYIGSYTARMKG
VDAIIFTAGVGENSAIIRERVLEGLEYMGVYFDAKRNNVFGEEAFINFPHSPVKIIVIPTDEEVMIARDVLRLGNIG

Sequences:

>Translated_397_residues
MSKIIAINAGSSSLKFQLFEMPSETVLTKGLVERIGLEDSIFTITVDGEKQKEITNIPDHAVAVNMLLKKLTENGIVKSL
DEIGGIGHRVVHGGEKFADSVLITDEVLADIEELSDLAPLHNPANVVGIKAFQEVLPNVPAVAVFDTAFHQTMPESAFLY
SLPYEYYEKFGIRKYGFHGTSHKYVTERAAELLGRPLESLSLLSCHLGNGASIAAVEGGKSIDTSMGFTPLAGVTMGTRS
GNIDPALIPYIMEKTGQTVEEVVNVLNKKSGMLGLTGYSSDLRDIIAKEEEGDHRAKVALDVFVSRIHKYIGSYTARMKG
VDAIIFTAGVGENSAIIRERVLEGLEYMGVYFDAKRNNVFGEEAFINFPHSPVKIIVIPTDEEVMIARDVLRLGNIG
>Mature_396_residues
SKIIAINAGSSSLKFQLFEMPSETVLTKGLVERIGLEDSIFTITVDGEKQKEITNIPDHAVAVNMLLKKLTENGIVKSLD
EIGGIGHRVVHGGEKFADSVLITDEVLADIEELSDLAPLHNPANVVGIKAFQEVLPNVPAVAVFDTAFHQTMPESAFLYS
LPYEYYEKFGIRKYGFHGTSHKYVTERAAELLGRPLESLSLLSCHLGNGASIAAVEGGKSIDTSMGFTPLAGVTMGTRSG
NIDPALIPYIMEKTGQTVEEVVNVLNKKSGMLGLTGYSSDLRDIIAKEEEGDHRAKVALDVFVSRIHKYIGSYTARMKGV
DAIIFTAGVGENSAIIRERVLEGLEYMGVYFDAKRNNVFGEEAFINFPHSPVKIIVIPTDEEVMIARDVLRLGNIG

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=399, Percent_Identity=44.6115288220551, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI145693184, Length=396, Percent_Identity=43.6868686868687, Blast_Score=335, Evalue=4e-93,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43232; Mature: 43101

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2 ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIIAINAGSSSLKFQLFEMPSETVLTKGLVERIGLEDSIFTITVDGEKQKEITNIPDH
CCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHCCCCHH
AVAVNMLLKKLTENGIVKSLDEIGGIGHRVVHGGEKFADSVLITDEVLADIEELSDLAPL
HHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHCCCHHHHCCEEEHHHHHHHHHHHHHHCCC
HNPANVVGIKAFQEVLPNVPAVAVFDTAFHQTMPESAFLYSLPYEYYEKFGIRKYGFHGT
CCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCEEEECCHHHHHHCCCEEECCCCC
SHKYVTERAAELLGRPLESLSLLSCHLGNGASIAAVEGGKSIDTSMGFTPLAGVTMGTRS
CCHHHHHHHHHHHCCCHHHHHHHHEECCCCCEEEEECCCCCCCCCCCCCCCCCEEECCCC
GNIDPALIPYIMEKTGQTVEEVVNVLNKKSGMLGLTGYSSDLRDIIAKEEEGDHRAKVAL
CCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCHHHHHHH
DVFVSRIHKYIGSYTARMKGVDAIIFTAGVGENSAIIRERVLEGLEYMGVYFDAKRNNVF
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCC
GEEAFINFPHSPVKIIVIPTDEEVMIARDVLRLGNIG
CCHHEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKIIAINAGSSSLKFQLFEMPSETVLTKGLVERIGLEDSIFTITVDGEKQKEITNIPDH
CEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHCCCCHH
AVAVNMLLKKLTENGIVKSLDEIGGIGHRVVHGGEKFADSVLITDEVLADIEELSDLAPL
HHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHCCCHHHHCCEEEHHHHHHHHHHHHHHCCC
HNPANVVGIKAFQEVLPNVPAVAVFDTAFHQTMPESAFLYSLPYEYYEKFGIRKYGFHGT
CCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCEEEECCHHHHHHCCCEEECCCCC
SHKYVTERAAELLGRPLESLSLLSCHLGNGASIAAVEGGKSIDTSMGFTPLAGVTMGTRS
CCHHHHHHHHHHHCCCHHHHHHHHEECCCCCEEEEECCCCCCCCCCCCCCCCCEEECCCC
GNIDPALIPYIMEKTGQTVEEVVNVLNKKSGMLGLTGYSSDLRDIIAKEEEGDHRAKVAL
CCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCHHHHHHH
DVFVSRIHKYIGSYTARMKGVDAIIFTAGVGENSAIIRERVLEGLEYMGVYFDAKRNNVF
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCC
GEEAFINFPHSPVKIIVIPTDEEVMIARDVLRLGNIG
CCHHEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]