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The map label for this gene is megL [H]

Identifier: 52140853

GI number: 52140853

Start: 4506676

End: 4507854

Strand: Reverse

Name: megL [H]

Synonym: BCZK4398

Alternate gene names: 52140853

Gene position: 4507854-4506676 (Counterclockwise)

Preceding gene: 52140850

Following gene: 52140855

Centisome position: 85.04

GC content: 38.85

Gene sequence:

>1179_bases
ATGAAAAAGAAGCATATGGAGACAGCGTTAATTCATCACGGTTATACATCAGAGGAACATAAAGGAAGTTTAACACCGCC
TTTATTTCAAACGTCTACATTTACATTTGAGACTGCGCAGCAAGGAGAGGCGAGTTTTGCGGGAGTGGATTCGTCTTATA
TTTACTCAAGACTTGGAAATCCGACTGTGAAACTATTTGAAGAACGTATGGCAGTGTTAGAAGGTGGAGAAGAAGCTCTT
GCTTTCGGATCCGGAATGGCAGCTATTTCAGCGACTTTAATTGGTTTTCTAAAAGCTGGAGATCATATTATTTGTTCAAA
TGGATTATACGGATGCACGTACGGCTTTTTAGAAGTGTTAGAAGAAAAATTTATGATTACGCATTCGTTTTGTGATATGG
AGACAGAGGCTGATATTGAAAATAAAATTCGCCCAAATACGAAGCTTATTTTCGTTGAAACACCGATTAATCCAACGATG
AAATTAATTGATTTAAAACAAGTAATTCGGGTTGCGAAGCGAAATGGGTTACTTGTTATTGTTGATAATACGTTTTGTTC
ACCTTATTTACAAAGGCCGCTTGAACTTGGCTGTGACGCGGTTGTTCATAGTGCGACAAAATATATTGGTGGTCACGGTG
ATGTTGTAGCGGGTGTAACAATTTGTAAAACGAGAGCGTTAGCTGAAAAAATTCGCCCGATGCGAAAAGATATCGGCGGT
ATTATGGCACCGTTTGATGCATGGTTATTGTTACGCGGATTAAAGACACTAGCGGTAAGAATGGATCGCCATTGTGATAA
TGCAGAAAAAATTGTATCGTTCCTAAAAAATCATGATGCGGTAGAAGGTGTTTGGTATCCAGAAGGAGAGTTAGCATCTC
GCCAAATGAAACGGGGCGGTGGCGTTATATCTTTTTCAATTAAAGGTGGGAAAGAAGAGACGCAAGCGTTTATTAATGAT
CTCCACTTTATTACAATTGCAGTAAGTTTAGGAGATACAGAAACGTTAATTCAGCATCCGGCAACGATGACGCACGCTGC
GATTCCAGCTGAGTTAAGACAAGAAATGGGCATTTATGATAATTTAATACGTTTATCTGTCGGTTTAGAATCGTGGGAGG
ATATCGTTTCTGATTTAGAGCAAGCGTTAAAGAAAATATCTACTGTTAGTAATCAATAA

Upstream 100 bases:

>100_bases
TAGAAACACAAATAAAACGCTTACATAAAATGAAGTAAAACGTTCATCAAATTTTTAATGATGAATATGCGGCAATAAAA
ACATAAAAGGGGATGTTTTT

Downstream 100 bases:

>100_bases
AAAAAGAGCTAGCTATCGGTTTTGTACGTAATGTACTTAATCGATAACTAGCTCTTTTTTGTTACATATGTTTTAGGAGT
GTTTCCACAAATTTTTCTAA

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDSSYIYSRLGNPTVKLFEERMAVLEGGEEAL
AFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVLEEKFMITHSFCDMETEADIENKIRPNTKLIFVETPINPTM
KLIDLKQVIRVAKRNGLLVIVDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTRALAEKIRPMRKDIGG
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGGGVISFSIKGGKEETQAFIND
LHFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYDNLIRLSVGLESWEDIVSDLEQALKKISTVSNQ

Sequences:

>Translated_392_residues
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDSSYIYSRLGNPTVKLFEERMAVLEGGEEAL
AFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVLEEKFMITHSFCDMETEADIENKIRPNTKLIFVETPINPTM
KLIDLKQVIRVAKRNGLLVIVDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTRALAEKIRPMRKDIGG
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGGGVISFSIKGGKEETQAFIND
LHFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYDNLIRLSVGLESWEDIVSDLEQALKKISTVSNQ
>Mature_392_residues
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDSSYIYSRLGNPTVKLFEERMAVLEGGEEAL
AFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVLEEKFMITHSFCDMETEADIENKIRPNTKLIFVETPINPTM
KLIDLKQVIRVAKRNGLLVIVDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTRALAEKIRPMRKDIGG
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGGGVISFSIKGGKEETQAFIND
LHFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYDNLIRLSVGLESWEDIVSDLEQALKKISTVSNQ

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=389, Percent_Identity=40.8740359897172, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI299473759, Length=389, Percent_Identity=36.5038560411311, Blast_Score=224, Evalue=8e-59,
Organism=Homo sapiens, GI25453487, Length=388, Percent_Identity=35.8247422680412, Blast_Score=203, Evalue=3e-52,
Organism=Escherichia coli, GI1790375, Length=390, Percent_Identity=36.4102564102564, Blast_Score=261, Evalue=7e-71,
Organism=Escherichia coli, GI1789383, Length=397, Percent_Identity=27.7078085642317, Blast_Score=175, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17560056, Length=390, Percent_Identity=40.2564102564103, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17538055, Length=390, Percent_Identity=41.2820512820513, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=39.4594594594595, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI193202265, Length=88, Percent_Identity=37.5, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6319307, Length=391, Percent_Identity=38.8746803069054, Blast_Score=267, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6323333, Length=446, Percent_Identity=30.0448430493274, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321254, Length=413, Percent_Identity=30.2663438256659, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6321904, Length=398, Percent_Identity=29.6482412060301, Blast_Score=149, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6321170, Length=320, Percent_Identity=30.3125, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI19922554, Length=385, Percent_Identity=38.1818181818182, Blast_Score=263, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 43242; Mature: 43242

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDSSYIYSRLGN
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCC
PTVKLFEERMAVLEGGEEALAFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVL
HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
EEKFMITHSFCDMETEADIENKIRPNTKLIFVETPINPTMKLIDLKQVIRVAKRNGLLVI
HHHHHHHHHHCCCCCCCCHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEE
VDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTRALAEKIRPMRKDIGG
ECCCCCCHHHHCHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHCC
GVISFSIKGGKEETQAFINDLHFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYD
CEEEEEECCCHHHHHHHHHCCEEEEEEEECCCHHHHHHCCCHHHHHCCCHHHHHHCCHHH
NLIRLSVGLESWEDIVSDLEQALKKISTVSNQ
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDSSYIYSRLGN
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCC
PTVKLFEERMAVLEGGEEALAFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVL
HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
EEKFMITHSFCDMETEADIENKIRPNTKLIFVETPINPTMKLIDLKQVIRVAKRNGLLVI
HHHHHHHHHHCCCCCCCCHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEE
VDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTRALAEKIRPMRKDIGG
ECCCCCCHHHHCHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHCC
GVISFSIKGGKEETQAFINDLHFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYD
CEEEEEECCCHHHHHHHHHCCEEEEEEEECCCHHHHHHCCCHHHHHCCCHHHHHHCCHHH
NLIRLSVGLESWEDIVSDLEQALKKISTVSNQ
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]