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The map label for this gene is walK [H]

Identifier: 52140833

GI number: 52140833

Start: 4523654

End: 4525108

Strand: Reverse

Name: walK [H]

Synonym: BCZK4418

Alternate gene names: 52140833

Gene position: 4525108-4523654 (Counterclockwise)

Preceding gene: 52140829

Following gene: 52140835

Centisome position: 85.36

GC content: 33.4

Gene sequence:

>1455_bases
ATGTCTATTAAAACAAGGTTTTTATTTTCTTATATCGCTGTCATTCTCGTTTCCATTACGCTTATATTAGTCGCGGGATT
TTTAATCGTTTTTTCGATAACAGGTGATTTGGAAGCAGTGAAAAATTTCTATAAAAGCTCCTATATTCAAAAGCCGCTTA
CGTCAGAAGAAGAGAATGCGTATATTGAGTTAAAGACGGCAGCAAAGAAACATCAATCGCAACTATTAGATGAATCGTTC
GTTTCATCACTTGAAAAAGAAGGGGTGAAAATCGTTGTAAGAAAGGGAGAAACCATTTCTTATGCTACAAAAGTATTTGA
AAGTGTGGCTTTAAAGGAAGCCCTTCCGAAATTTGAATCAGCAAATATTAATAGTCGTGGTACAACGGAATTAGGCGATA
CATTTTATCGATATGTAAAGTTTGATTTTTATTTTCCTCAGGAAGAAGAGGGGAGTATATTTGTATTAAAGAAGCAAAGT
TCATTTGTAGATCTTACACAAAAGTTATTTCCGATTTTGTTCGTATCACTTTTACTGTTAGCTATTTTACTTATTGGATT
ATTAAGTTACCTCGTCTCAAGAAGTGTTATAAAACCAATCTTTGTATTGAAAGATGCGACTGAAAAAATTAAAGAAGGAA
ATTTAGATTTTCAAATACCAGTTACATCGCACGATGAAATTGGGCAATTGAATCAAGGATTTGAGGAAATGAGGAAGAAA
TTAAAAGAATCGATAGAGATGCAAACGCAGTATGAAGAAAATCGAAAAGAGCTCATTTCAAACATCTCTCACGATTTAAA
AACACCGATTACATCTATTATTGGATATGTAGAAGGAATAAAAGACGGAGTAGCAAATACGCCAGAAAAAATGGATAAGT
ACTTAACGACTATCCATACGAAAGCAAGACATATGGATACACTCATTGATGAATTATTTTTATTCTCAAAGCTCGATTTG
AATCGAGTTCCCTTTCAGTTTGAAACGGTTGAATTAAATATGTTTATGCAAGAATTAATAGAAGAGATGCAGATGGATTT
AAGTAAAGAATGTATAGAAGTTAACTTACAATTACATGCATCACCACTTTATGTAAAGGCTGATTGCGAAAAGATAAATA
GAGTGATATCAAATTTAATTCATAATAGTGTGAAATACATGGATAAAGAAGAAAAGAAAATTACTGTAACAGTATCGAGT
GATAACAATAAAGTGATTGTGAAAGTAATGGACAACGGATCAGGTATAGAATCTGATACACTTCCTTATATTTTTGAACG
TTTCTATCGTGCAGAGAAATCGCGAAATTCTAGTACAGGTGGAAGTGGACTTGGCTTAGCGATAGCGAAGCAAATTATTG
AAGAACATGGCGGGAATATTTGGGCGGAAAGCGAGCTTGGGGAAGGCACAAGCATTTTCTTCTCATTGGAAAAAGTAGAG
AAATGTGGTGAGTAA

Upstream 100 bases:

>100_bases
TATAAACTGACGCTAAAATTACTATAAAATCGCTTGAGTATTTGAACATATATTTTTATGCTTATGAGTAATAAGGTTTG
ATAAAGTGAGGATGTAACAT

Downstream 100 bases:

>100_bases
ATTGAAAAGAATTTTACTAATAGAAGATGAAGTAAGCATTGCAGAATTACAGCGAGATTATTTAGAAATTAATGATTTTC
AAGTTGATGTAGAACATTCA

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSIKTRFLFSYIAVILVSITLILVAGFLIVFSITGDLEAVKNFYKSSYIQKPLTSEEENAYIELKTAAKKHQSQLLDESF
VSSLEKEGVKIVVRKGETISYATKVFESVALKEALPKFESANINSRGTTELGDTFYRYVKFDFYFPQEEEGSIFVLKKQS
SFVDLTQKLFPILFVSLLLLAILLIGLLSYLVSRSVIKPIFVLKDATEKIKEGNLDFQIPVTSHDEIGQLNQGFEEMRKK
LKESIEMQTQYEENRKELISNISHDLKTPITSIIGYVEGIKDGVANTPEKMDKYLTTIHTKARHMDTLIDELFLFSKLDL
NRVPFQFETVELNMFMQELIEEMQMDLSKECIEVNLQLHASPLYVKADCEKINRVISNLIHNSVKYMDKEEKKITVTVSS
DNNKVIVKVMDNGSGIESDTLPYIFERFYRAEKSRNSSTGGSGLGLAIAKQIIEEHGGNIWAESELGEGTSIFFSLEKVE
KCGE

Sequences:

>Translated_484_residues
MSIKTRFLFSYIAVILVSITLILVAGFLIVFSITGDLEAVKNFYKSSYIQKPLTSEEENAYIELKTAAKKHQSQLLDESF
VSSLEKEGVKIVVRKGETISYATKVFESVALKEALPKFESANINSRGTTELGDTFYRYVKFDFYFPQEEEGSIFVLKKQS
SFVDLTQKLFPILFVSLLLLAILLIGLLSYLVSRSVIKPIFVLKDATEKIKEGNLDFQIPVTSHDEIGQLNQGFEEMRKK
LKESIEMQTQYEENRKELISNISHDLKTPITSIIGYVEGIKDGVANTPEKMDKYLTTIHTKARHMDTLIDELFLFSKLDL
NRVPFQFETVELNMFMQELIEEMQMDLSKECIEVNLQLHASPLYVKADCEKINRVISNLIHNSVKYMDKEEKKITVTVSS
DNNKVIVKVMDNGSGIESDTLPYIFERFYRAEKSRNSSTGGSGLGLAIAKQIIEEHGGNIWAESELGEGTSIFFSLEKVE
KCGE
>Mature_483_residues
SIKTRFLFSYIAVILVSITLILVAGFLIVFSITGDLEAVKNFYKSSYIQKPLTSEEENAYIELKTAAKKHQSQLLDESFV
SSLEKEGVKIVVRKGETISYATKVFESVALKEALPKFESANINSRGTTELGDTFYRYVKFDFYFPQEEEGSIFVLKKQSS
FVDLTQKLFPILFVSLLLLAILLIGLLSYLVSRSVIKPIFVLKDATEKIKEGNLDFQIPVTSHDEIGQLNQGFEEMRKKL
KESIEMQTQYEENRKELISNISHDLKTPITSIIGYVEGIKDGVANTPEKMDKYLTTIHTKARHMDTLIDELFLFSKLDLN
RVPFQFETVELNMFMQELIEEMQMDLSKECIEVNLQLHASPLYVKADCEKINRVISNLIHNSVKYMDKEEKKITVTVSSD
NNKVIVKVMDNGSGIESDTLPYIFERFYRAEKSRNSSTGGSGLGLAIAKQIIEEHGGNIWAESELGEGTSIFFSLEKVEK
CGE

Specific function: Member of the two-component regulatory system walK/walR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=280, Percent_Identity=30.3571428571429, Blast_Score=138, Evalue=9e-34,
Organism=Escherichia coli, GI1786600, Length=225, Percent_Identity=31.5555555555556, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1790346, Length=273, Percent_Identity=26.3736263736264, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789149, Length=342, Percent_Identity=24.2690058479532, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994928, Length=242, Percent_Identity=26.4462809917355, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790861, Length=295, Percent_Identity=28.135593220339, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787894, Length=344, Percent_Identity=23.8372093023256, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081816, Length=337, Percent_Identity=24.3323442136499, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1786783, Length=327, Percent_Identity=26.2996941896024, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI145693157, Length=243, Percent_Identity=23.8683127572016, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1786912, Length=232, Percent_Identity=26.7241379310345, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789808, Length=298, Percent_Identity=24.8322147651007, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87082128, Length=311, Percent_Identity=24.7588424437299, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788713, Length=221, Percent_Identity=26.2443438914027, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1788279, Length=292, Percent_Identity=25.6849315068493, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1790436, Length=234, Percent_Identity=24.3589743589744, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788549, Length=216, Percent_Identity=26.3888888888889, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789403, Length=205, Percent_Identity=27.3170731707317, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54958; Mature: 54827

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKTRFLFSYIAVILVSITLILVAGFLIVFSITGDLEAVKNFYKSSYIQKPLTSEEENA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCC
YIELKTAAKKHQSQLLDESFVSSLEKEGVKIVVRKGETISYATKVFESVALKEALPKFES
EEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCC
ANINSRGTTELGDTFYRYVKFDFYFPQEEEGSIFVLKKQSSFVDLTQKLFPILFVSLLLL
CCCCCCCCCHHHHHHHHHHHEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
AILLIGLLSYLVSRSVIKPIFVLKDATEKIKEGNLDFQIPVTSHDEIGQLNQGFEEMRKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHH
LKESIEMQTQYEENRKELISNISHDLKTPITSIIGYVEGIKDGVANTPEKMDKYLTTIHT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KARHMDTLIDELFLFSKLDLNRVPFQFETVELNMFMQELIEEMQMDLSKECIEVNLQLHA
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEC
SPLYVKADCEKINRVISNLIHNSVKYMDKEEKKITVTVSSDNNKVIVKVMDNGSGIESDT
CCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCC
LPYIFERFYRAEKSRNSSTGGSGLGLAIAKQIIEEHGGNIWAESELGEGTSIFFSLEKVE
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEHHHHH
KCGE
HCCC
>Mature Secondary Structure 
SIKTRFLFSYIAVILVSITLILVAGFLIVFSITGDLEAVKNFYKSSYIQKPLTSEEENA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCC
YIELKTAAKKHQSQLLDESFVSSLEKEGVKIVVRKGETISYATKVFESVALKEALPKFES
EEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCC
ANINSRGTTELGDTFYRYVKFDFYFPQEEEGSIFVLKKQSSFVDLTQKLFPILFVSLLLL
CCCCCCCCCHHHHHHHHHHHEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
AILLIGLLSYLVSRSVIKPIFVLKDATEKIKEGNLDFQIPVTSHDEIGQLNQGFEEMRKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHH
LKESIEMQTQYEENRKELISNISHDLKTPITSIIGYVEGIKDGVANTPEKMDKYLTTIHT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KARHMDTLIDELFLFSKLDLNRVPFQFETVELNMFMQELIEEMQMDLSKECIEVNLQLHA
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEC
SPLYVKADCEKINRVISNLIHNSVKYMDKEEKKITVTVSSDNNKVIVKVMDNGSGIESDT
CCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCC
LPYIFERFYRAEKSRNSSTGGSGLGLAIAKQIIEEHGGNIWAESELGEGTSIFFSLEKVE
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEHHHHH
KCGE
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA