BacMap

Definition	Bacillus cereus E33L, complete genome.
Accession	NC_006274
Length	5,300,915

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The map label for this gene is nodI [H]

Identifier: 52140752

GI number: 52140752

Start: 4598341

End: 4599174

Strand: Direct

Name: nodI [H]

Synonym: BCZK4501

Alternate gene names: 52140752

Gene position: 4598341-4599174 (Clockwise)

Preceding gene: 52140754

Following gene: 52140753

Centisome position: 86.75

GC content: 34.53

Gene sequence:

>834_bases
TTGCTAGTAGAACTAAGGGGAATTCAAAAGAAATATGGCAAATCACTTATACTAGACAACATTGATTTATCCATTCCAGA
AGGAGAAGCGCTCGCTATTATTGGCGGGAACGGGACTGGAAAAAGTACTCTACTCAAAATAATTGCAGGCTTTATTTCAC
CTACGGCAGGGACAATTCAAAGAAAAGAACATATACAAATCGGTTATGTACCTGAACATTTTCCTGAAGGGATTCGTTTT
ACATTAGAAGATTATCTATATCATCTCGGCCACATTCACGGTTTATCAACAAAATATGTAAAAGATAAAATTCCGATGCT
TCTGGAATCTTTTCATTTACATCATGCAAGACATTCTGTTGTACGAAACTTTTCAAAAGGTATGAAACAAAAAACAGGCA
TTATGCAAGCGTTACTTACGGACGTACATTTATTAATTTTAGACGAACCGCTTTCTGGGCTCGATCCTAACTCTCAGCAA
GAATTAGAACATATTTTACTCTCATTAAAACAACAAGGTATATCTATACTCTTCACATGTCACGAAAAACAGCTACTAGA
AAACTTCGCCGATAGAATTGTGACGTTAGCAAATCATACAATTGCAGAAAATATCTCTGCGCAAAAAGGAGCAGAACAAG
TCTATATTGAGGCAATCGTTCACGAAACATTTTCAACAATAGAACTACAAAAGCAATCCGGTTTTATACACGTTGCACAC
ACTTCAAATCAAAATCTTATTCAATTACAAATCGAAAAAGAATATACAAACGAAATGCTTCAGTTTTTATTACATAAAAA
AGCATCTATCACACTGCTACAACCTAACTTTTAA

Upstream 100 bases:

>100_bases
ACAAAACATAACAAAAAAAGACGTGCGCTCATTGCACGTCTTTTTGTTATTCTCCTATATAATGGATATATATACTATTT
AAAGAAAGAAGGATAGAACT

Downstream 100 bases:

>100_bases
ATCGAAAGAAGGTTTATTATGTTTGCTCTTATTCGTTATCACTTTCTCGACTATACAAAATCGTATAAGTACGTCCCTCC
TATTGCGATGTACTTTATGA

Product: ABC transporter, ATP-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Nodulation ATP-binding protein I [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MLVELRGIQKKYGKSLILDNIDLSIPEGEALAIIGGNGTGKSTLLKIIAGFISPTAGTIQRKEHIQIGYVPEHFPEGIRF
TLEDYLYHLGHIHGLSTKYVKDKIPMLLESFHLHHARHSVVRNFSKGMKQKTGIMQALLTDVHLLILDEPLSGLDPNSQQ
ELEHILLSLKQQGISILFTCHEKQLLENFADRIVTLANHTIAENISAQKGAEQVYIEAIVHETFSTIELQKQSGFIHVAH
TSNQNLIQLQIEKEYTNEMLQFLLHKKASITLLQPNF

Sequences:

>Translated_277_residues
MLVELRGIQKKYGKSLILDNIDLSIPEGEALAIIGGNGTGKSTLLKIIAGFISPTAGTIQRKEHIQIGYVPEHFPEGIRF
TLEDYLYHLGHIHGLSTKYVKDKIPMLLESFHLHHARHSVVRNFSKGMKQKTGIMQALLTDVHLLILDEPLSGLDPNSQQ
ELEHILLSLKQQGISILFTCHEKQLLENFADRIVTLANHTIAENISAQKGAEQVYIEAIVHETFSTIELQKQSGFIHVAH
TSNQNLIQLQIEKEYTNEMLQFLLHKKASITLLQPNF
>Mature_277_residues
MLVELRGIQKKYGKSLILDNIDLSIPEGEALAIIGGNGTGKSTLLKIIAGFISPTAGTIQRKEHIQIGYVPEHFPEGIRF
TLEDYLYHLGHIHGLSTKYVKDKIPMLLESFHLHHARHSVVRNFSKGMKQKTGIMQALLTDVHLLILDEPLSGLDPNSQQ
ELEHILLSLKQQGISILFTCHEKQLLENFADRIVTLANHTIAENISAQKGAEQVYIEAIVHETFSTIELQKQSGFIHVAH
TSNQNLIQLQIEKEYTNEMLQFLLHKKASITLLQPNF

Specific function: Part of the ABC transporter complex NodIJ involved in the export of the nodulation factors (Nod factors), the bacterial signal molecules that induce symbiosis and subsequent nodulation induction. Nod factors are LCO (lipo-chitin oligosaccharide), a modifi

COG id: COG1131

COG function: function code V; ABC-type multidrug transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27881501, Length=212, Percent_Identity=30.6603773584906, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI30795238, Length=212, Percent_Identity=30.6603773584906, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI47078218, Length=198, Percent_Identity=28.2828282828283, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI45446740, Length=198, Percent_Identity=28.2828282828283, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI153792144, Length=201, Percent_Identity=28.3582089552239, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI27436953, Length=193, Percent_Identity=29.5336787564767, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI105990541, Length=190, Percent_Identity=27.8947368421053, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI21536376, Length=209, Percent_Identity=28.2296650717703, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI31657092, Length=204, Percent_Identity=27.4509803921569, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI6005701, Length=200, Percent_Identity=27, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI27262624, Length=190, Percent_Identity=25.7894736842105, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI27262626, Length=190, Percent_Identity=25.7894736842105, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI27477115, Length=195, Percent_Identity=26.6666666666667, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI116734710, Length=188, Percent_Identity=29.2553191489362, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI150417984, Length=207, Percent_Identity=26.0869565217391, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1788761, Length=231, Percent_Identity=29.4372294372294, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1788165, Length=231, Percent_Identity=26.8398268398268, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI87081782, Length=202, Percent_Identity=27.2277227722772, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787792, Length=209, Percent_Identity=31.1004784688995, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787029, Length=221, Percent_Identity=27.6018099547511, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1789593, Length=189, Percent_Identity=26.984126984127, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI48994943, Length=247, Percent_Identity=29.1497975708502, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081709, Length=202, Percent_Identity=27.2277227722772, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790739, Length=244, Percent_Identity=24.5901639344262, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787164, Length=216, Percent_Identity=28.2407407407407, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786319, Length=244, Percent_Identity=27.0491803278689, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788225, Length=228, Percent_Identity=28.5087719298246, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI87081791, Length=206, Percent_Identity=26.2135922330097, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787089, Length=225, Percent_Identity=26.6666666666667, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789751, Length=191, Percent_Identity=29.3193717277487, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1786253, Length=193, Percent_Identity=30.5699481865285, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790467, Length=211, Percent_Identity=26.5402843601896, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789672, Length=219, Percent_Identity=25.5707762557078, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786398, Length=226, Percent_Identity=26.1061946902655, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787712, Length=214, Percent_Identity=27.1028037383178, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787370, Length=223, Percent_Identity=28.2511210762332, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI48995001, Length=209, Percent_Identity=28.2296650717703, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1789032, Length=212, Percent_Identity=26.4150943396226, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787500, Length=199, Percent_Identity=27.1356783919598, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787105, Length=257, Percent_Identity=26.0700389105058, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790544, Length=236, Percent_Identity=26.271186440678, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786345, Length=225, Percent_Identity=25.7777777777778, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789586, Length=223, Percent_Identity=27.3542600896861, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787041, Length=201, Percent_Identity=25.3731343283582, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790525, Length=220, Percent_Identity=25, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082268, Length=202, Percent_Identity=30.6930693069307, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786803, Length=213, Percent_Identity=26.7605633802817, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786872, Length=226, Percent_Identity=26.1061946902655, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI2367384, Length=203, Percent_Identity=28.0788177339901, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788540, Length=220, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082267, Length=219, Percent_Identity=25.5707762557078, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081834, Length=198, Percent_Identity=25.7575757575758, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1790190, Length=224, Percent_Identity=26.7857142857143, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1789991, Length=214, Percent_Identity=26.6355140186916, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787182, Length=227, Percent_Identity=23.3480176211454, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1788450, Length=216, Percent_Identity=24.0740740740741, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI48994997, Length=221, Percent_Identity=24.4343891402715, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786703, Length=224, Percent_Identity=24.5535714285714, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=193, Percent_Identity=28.4974093264249, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI115533608, Length=206, Percent_Identity=25.7281553398058, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71983523, Length=211, Percent_Identity=27.9620853080569, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71983516, Length=211, Percent_Identity=27.9620853080569, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71983510, Length=211, Percent_Identity=27.9620853080569, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17565586, Length=241, Percent_Identity=24.896265560166, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17559834, Length=196, Percent_Identity=25, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI212646699, Length=223, Percent_Identity=24.2152466367713, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321121, Length=216, Percent_Identity=23.6111111111111, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320874, Length=181, Percent_Identity=23.7569060773481, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24666092, Length=206, Percent_Identity=31.5533980582524, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI116007328, Length=207, Percent_Identity=30.4347826086957, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28574150, Length=207, Percent_Identity=30.4347826086957, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24643674, Length=206, Percent_Identity=24.2718446601942, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI85725262, Length=195, Percent_Identity=29.7435897435897, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24650853, Length=195, Percent_Identity=29.2307692307692, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24650855, Length=195, Percent_Identity=29.2307692307692, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281362751, Length=195, Percent_Identity=29.2307692307692, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI221512771, Length=206, Percent_Identity=27.1844660194175, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28573571, Length=187, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28571209, Length=207, Percent_Identity=27.536231884058, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28573573, Length=187, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24582014, Length=195, Percent_Identity=25.1282051282051, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24582012, Length=195, Percent_Identity=25.1282051282051, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648314, Length=209, Percent_Identity=23.444976076555, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI221500365, Length=194, Percent_Identity=24.7422680412371, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI116007184, Length=194, Percent_Identity=24.7422680412371, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24581191, Length=190, Percent_Identity=31.0526315789474, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24581189, Length=190, Percent_Identity=31.0526315789474, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24581193, Length=190, Percent_Identity=31.0526315789474, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24581195, Length=190, Percent_Identity=31.0526315789474, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24662800, Length=197, Percent_Identity=28.9340101522843, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI85816269, Length=204, Percent_Identity=28.921568627451, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24580553, Length=204, Percent_Identity=28.921568627451, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI85724864, Length=204, Percent_Identity=28.921568627451, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24580555, Length=204, Percent_Identity=28.921568627451, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI19920532, Length=205, Percent_Identity=27.3170731707317, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24580930, Length=205, Percent_Identity=27.3170731707317, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR015851
- InterPro:   IPR005978
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: 3.6.3.25 [C]

Molecular weight: Translated: 31205; Mature: 31205

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVELRGIQKKYGKSLILDNIDLSIPEGEALAIIGGNGTGKSTLLKIIAGFISPTAGTIQ
CEEEECCHHHHHCCEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCC
RKEHIQIGYVPEHFPEGIRFTLEDYLYHLGHIHGLSTKYVKDKIPMLLESFHLHHARHSV
CCCCEEECCCHHHCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VRNFSKGMKQKTGIMQALLTDVHLLILDEPLSGLDPNSQQELEHILLSLKQQGISILFTC
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEE
HEKQLLENFADRIVTLANHTIAENISAQKGAEQVYIEAIVHETFSTIELQKQSGFIHVAH
CHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHEEEECCCCEEEEEE
TSNQNLIQLQIEKEYTNEMLQFLLHKKASITLLQPNF
CCCCCEEEEEECHHHHHHHHHHHHHCCCEEEEECCCC
>Mature Secondary Structure
MLVELRGIQKKYGKSLILDNIDLSIPEGEALAIIGGNGTGKSTLLKIIAGFISPTAGTIQ
CEEEECCHHHHHCCEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCC
RKEHIQIGYVPEHFPEGIRFTLEDYLYHLGHIHGLSTKYVKDKIPMLLESFHLHHARHSV
CCCCEEECCCHHHCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VRNFSKGMKQKTGIMQALLTDVHLLILDEPLSGLDPNSQQELEHILLSLKQQGISILFTC
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEE
HEKQLLENFADRIVTLANHTIAENISAQKGAEQVYIEAIVHETFSTIELQKQSGFIHVAH
CHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHEEEECCCCEEEEEE
TSNQNLIQLQIEKEYTNEMLQFLLHKKASITLLQPNF
CCCCCEEEEEECHHHHHHHHHHHHHCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2134855; 11157954; 12597275 [H]