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The map label for this gene is vanS [H]

Identifier: 52140650

GI number: 52140650

Start: 4691737

End: 4692843

Strand: Reverse

Name: vanS [H]

Synonym: BCZK4604

Alternate gene names: 52140650

Gene position: 4692843-4691737 (Counterclockwise)

Preceding gene: 52140648

Following gene: 52140652

Centisome position: 88.53

GC content: 34.51

Gene sequence:

>1107_bases
ATGGGTAAAAAGATGAGAAGTTTTCGCTCAAAGATGGTTATGCTATTTGCGTTAAGTATGCTATTTGCTGGTGGCGTAAC
GTATATCATTTATGAAGGGTTACGGATCTATTATAAATCAGTCGTTCGTTTTGAAGATCCGTTAGCTCAATTGCGTTTCA
TGATAAGACAAATTGGGGATGTTAATTTCTTTTTAATTATTTTCATACCTTTGTCTATTATGTTTTTCTTCTTCCTTACT
AGACCGTATTTAAAATATTTTGATGAGATTTCAAACGGAATTCATCATTTGGCGAACGGAGATTTTACAAATGAAGTGCG
CGTTTCATCAAATGATGAGTTTGGAGACATCGCGCGTGAAATAAATGTCGCGAGTGAAAAGTTAAAAGAAGCGGTGGAAA
GAGGGGACTTTGCTGAAAGTAGTAAAGACCAGCTTGTTGTGAATTTAGCTCATGATTTAAGGACACCATTAACATCCGTG
TTAGGGTATTTAGATTTAATTCTTAAAGATGAGAATTTAACAAAAGAACAAATTAAACATTTTTCCACGATTGCATATAC
GAAATCGCAAAGGCTTGAAAGCTTAATTGATGAATTATTTGAAATTACACGTATGAATTATGGCATGCTAAAGCTGGATA
AAAAGCAGATTGATATAAGTGAGCTACTTATACAGTTAGATGAGGAATTGTATCCGTTATTAGAAAAACATCATTTAGAA
GCTAGATTGAATATTGATCCGCATTTGCCGATTAACGGTGATGGAAAATTGTTAGCGAGAGTATTTGAAAACTTGTTAAC
AAATGCCGTTCGATACGGATATGATGGGCAATTTGTTGATCTGAATGGGTATATCGATAATGGAGAAGTTGTCGTACAAG
TTATAAATTATGGAGATAGCATTCCAGAAGAAGATTTACCGTATCTTTTTGATATGTTTTATACAGGTGATAAATCGAGA
ACTGAGAACCGTGGCGGGACAGGCCTTGGGCTATTTATTGCAAAAAATATTATCGAGCAGCATAACGGTACGATTTCTGC
TGAAAGTAATGTAGTTAGAACAATGTTTGAAGTGCGATTGCCAAAAGACGAAAACGTAACAATTTAA

Upstream 100 bases:

>100_bases
ACAGTTATGGTACATATTCGTACGTTGCGGAAAAAGCTTGGGGAGGATAAAAGAAAAGATAAGCTAATAAAAACAGTTTG
GGGAGTAGGGTATACTTTCA

Downstream 100 bases:

>100_bases
TAAAAATTTAAACTTTACCCCACTTTTTATTTTAATAGTTTTTTCTATTCTATACGTAAGTTACGTTAAGGAGGAACTAG
AAATGAAAAAGTGGGTATTT

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MGKKMRSFRSKMVMLFALSMLFAGGVTYIIYEGLRIYYKSVVRFEDPLAQLRFMIRQIGDVNFFLIIFIPLSIMFFFFLT
RPYLKYFDEISNGIHHLANGDFTNEVRVSSNDEFGDIAREINVASEKLKEAVERGDFAESSKDQLVVNLAHDLRTPLTSV
LGYLDLILKDENLTKEQIKHFSTIAYTKSQRLESLIDELFEITRMNYGMLKLDKKQIDISELLIQLDEELYPLLEKHHLE
ARLNIDPHLPINGDGKLLARVFENLLTNAVRYGYDGQFVDLNGYIDNGEVVVQVINYGDSIPEEDLPYLFDMFYTGDKSR
TENRGGTGLGLFIAKNIIEQHNGTISAESNVVRTMFEVRLPKDENVTI

Sequences:

>Translated_368_residues
MGKKMRSFRSKMVMLFALSMLFAGGVTYIIYEGLRIYYKSVVRFEDPLAQLRFMIRQIGDVNFFLIIFIPLSIMFFFFLT
RPYLKYFDEISNGIHHLANGDFTNEVRVSSNDEFGDIAREINVASEKLKEAVERGDFAESSKDQLVVNLAHDLRTPLTSV
LGYLDLILKDENLTKEQIKHFSTIAYTKSQRLESLIDELFEITRMNYGMLKLDKKQIDISELLIQLDEELYPLLEKHHLE
ARLNIDPHLPINGDGKLLARVFENLLTNAVRYGYDGQFVDLNGYIDNGEVVVQVINYGDSIPEEDLPYLFDMFYTGDKSR
TENRGGTGLGLFIAKNIIEQHNGTISAESNVVRTMFEVRLPKDENVTI
>Mature_367_residues
GKKMRSFRSKMVMLFALSMLFAGGVTYIIYEGLRIYYKSVVRFEDPLAQLRFMIRQIGDVNFFLIIFIPLSIMFFFFLTR
PYLKYFDEISNGIHHLANGDFTNEVRVSSNDEFGDIAREINVASEKLKEAVERGDFAESSKDQLVVNLAHDLRTPLTSVL
GYLDLILKDENLTKEQIKHFSTIAYTKSQRLESLIDELFEITRMNYGMLKLDKKQIDISELLIQLDEELYPLLEKHHLEA
RLNIDPHLPINGDGKLLARVFENLLTNAVRYGYDGQFVDLNGYIDNGEVVVQVINYGDSIPEEDLPYLFDMFYTGDKSRT
ENRGGTGLGLFIAKNIIEQHNGTISAESNVVRTMFEVRLPKDENVTI

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=287, Percent_Identity=29.2682926829268, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI145693157, Length=239, Percent_Identity=29.7071129707113, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082128, Length=318, Percent_Identity=29.2452830188679, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789149, Length=303, Percent_Identity=28.3828382838284, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786783, Length=233, Percent_Identity=27.8969957081545, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788713, Length=295, Percent_Identity=26.1016949152542, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1786600, Length=227, Percent_Identity=27.7533039647577, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994928, Length=230, Percent_Identity=29.1304347826087, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1786912, Length=232, Percent_Identity=28.448275862069, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1790346, Length=210, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787894, Length=310, Percent_Identity=24.5161290322581, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788279, Length=311, Percent_Identity=26.3665594855305, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87081816, Length=349, Percent_Identity=25.214899713467, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788549, Length=311, Percent_Identity=25.7234726688103, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790861, Length=229, Percent_Identity=22.707423580786, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790436, Length=219, Percent_Identity=24.6575342465753, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42334; Mature: 42203

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKMRSFRSKMVMLFALSMLFAGGVTYIIYEGLRIYYKSVVRFEDPLAQLRFMIRQIGD
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VNFFLIIFIPLSIMFFFFLTRPYLKYFDEISNGIHHLANGDFTNEVRVSSNDEFGDIARE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHH
INVASEKLKEAVERGDFAESSKDQLVVNLAHDLRTPLTSVLGYLDLILKDENLTKEQIKH
HHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
FSTIAYTKSQRLESLIDELFEITRMNYGMLKLDKKQIDISELLIQLDEELYPLLEKHHLE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHCCHHHHHHHHHHHHHHHHHHHCCC
ARLNIDPHLPINGDGKLLARVFENLLTNAVRYGYDGQFVDLNGYIDNGEVVVQVINYGDS
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCEECCCCEEEEEECCCCC
IPEEDLPYLFDMFYTGDKSRTENRGGTGLGLFIAKNIIEQHNGTISAESNVVRTMFEVRL
CCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
PKDENVTI
CCCCCCCC
>Mature Secondary Structure 
GKKMRSFRSKMVMLFALSMLFAGGVTYIIYEGLRIYYKSVVRFEDPLAQLRFMIRQIGD
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VNFFLIIFIPLSIMFFFFLTRPYLKYFDEISNGIHHLANGDFTNEVRVSSNDEFGDIARE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHH
INVASEKLKEAVERGDFAESSKDQLVVNLAHDLRTPLTSVLGYLDLILKDENLTKEQIKH
HHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
FSTIAYTKSQRLESLIDELFEITRMNYGMLKLDKKQIDISELLIQLDEELYPLLEKHHLE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHCCHHHHHHHHHHHHHHHHHHHCCC
ARLNIDPHLPINGDGKLLARVFENLLTNAVRYGYDGQFVDLNGYIDNGEVVVQVINYGDS
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCEECCCCEEEEEECCCCC
IPEEDLPYLFDMFYTGDKSRTENRGGTGLGLFIAKNIIEQHNGTISAESNVVRTMFEVRL
CCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
PKDENVTI
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]