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The map label for this gene is menD [H]

Identifier: 52140644

GI number: 52140644

Start: 4698151

End: 4699905

Strand: Reverse

Name: menD [H]

Synonym: BCZK4610

Alternate gene names: 52140644

Gene position: 4699905-4698151 (Counterclockwise)

Preceding gene: 52140643

Following gene: 52140646

Centisome position: 88.66

GC content: 40.91

Gene sequence:

>1755_bases
ATGAATAATCATATAGAAGCATTATCATATTATTTAGGCGCGTTCGTGGATGAACTGACGCGTCTAAATGTATGTGATGT
TGTCATTAGTCCAGGCTCACGGTCAACGCCGATTGCTCTACTAATGGAACAACACGAAGGAATGAACACATATTTACATG
TAGATGAAAGATCTGCAGGATTTTTTGCGCTCGGTATCGCAAAAGCGAAAAAACGTCCGGTTGCATTATTATGTACGTCA
GGAACGGCAGCAGCGAACTACTATCCAGCTGTATGTGAAGCTTTCCATTCACGAGTGCCGCTTATCGTCTTAACGGCAGA
TAGACCGCATGAATTAAGAGATGTCGGTGCACCACAAGCGATGAATCAATTTAATTTATACGGTACGTTTGTGAAGCAAT
TTACAGAGATGGCACTTCCAGAAGCGAGTGAAGCGATGTACCACTACGCTCGCATGACAACGCAGCGCATGATAGCAAGT
GCTTGTTTAGCGCCGCAAGGACCTGTTCATCTTAATTTTCCAGTTCGTGAACCGCTTATCCCGGACTTCTCATTAGAAAG
TTTATGGGATAAAGGACGCGGGGAATATACAGGAGTAGTTCAGCAAGGGAACGTGGTGATGCCGAGTGAATATGTAGATT
CTCTTGTAGGGCGCCTTTCACATATGGAAAAGGGGCTTATTATTTGTGGAGATGATAGTCATTCAGAAATCGCAGCATTT
GCAACGCAACTAGCTGAAAAAACGGGATATCCAATATTAGCGGATCCACTTTCTAACATTCGTAGCGGACACCACGATAA
AACGATGGTAATCGACTGTTACGATACATTTTTACGAAATGAGCTGTTAAAGGAAACGTGGAAGCCGGACGTTTTAATTC
GCTTCGGTGGTATGCCTGTTTCAAAAGCATTAACGCAGTTCATCAAAAAACAAACGAAAGCTGTTCATATCGTAGTTGAC
GAATCAGGACAATGGAGAGATCCAGCTCTCGTTGCAACAGAAGTTGTTCAAGCAAGTGATATTGCATTTTGCAGTGCACT
AATAGAAAAAATGCCAGTTATGAAAAAGAATGATTGGTTCCGAATGTGGCAACATATAAACGAAAAAACGAAGGAAACGC
TTCGTGAAATGGAAACATATGATACGGCGTTTGAAGGAAGAGTCATTACGGATATTGTCCGCGTATTACCAGAAGGGGCA
ACGTTATTTGCGAGTAATAGTATGCCAATTCGTGATACAGATTCATTCTTTTTCACATCGGATAAAAATATTCAAGTGAT
GGCTAACCGTGGTGTAAATGGGATTGATGGAATCATTTCGACAGCTTTAGGAGCGAGCATTATTTGCGATCCGCTCGTAT
TAGTAATCGGTGATTTATCATTTTATCACGATTTAAATGGATTATTAGCCGCAAAATTACATGAATTAAATATAACAATT
GTCGTTGTAAATAATGACGGTGGTGGTATTTTCTCATTCTTACCGCAATATGAGAAAAAGGAACACTTCGAATCATTATT
CGGAACTCCAATTGGGCTTGATTATGAGCACGTTGTTACAATGTATGGCGGTTCATTTAGCCGTGTAAATGGTTGGGAGC
AATTCCGTGAAGAAGTACAAAAGGGAGCAACGACAGAAGGTTTACACGTTGTGGAAATTTGTACGAACCGTGATGAAAAC
TTAACATTGCACCGTACGTTATGGGCAAAAACACAGGACGTAATTACTACATCGTTGCAAGGTGAATCAAAATGA

Upstream 100 bases:

>100_bases
CCGGTTGCGGGATTGTAATTGATTCAGTACCGCAACTTGAATATGAAGAGACAAGTTTGAAGTTTAGACCGATGCTTGGT
GCTTTGGAGGAATTAATGAA

Downstream 100 bases:

>100_bases
ACGTAACGCTGCAAGGTGTATCGTATGAATATGAAGTAGTCGGAAGCGGAGAGCCACTTCTACTTCTTCATGGTTTTACG
GGAAGCATGGAAACGTGGCG

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MNNHIEALSYYLGAFVDELTRLNVCDVVISPGSRSTPIALLMEQHEGMNTYLHVDERSAGFFALGIAKAKKRPVALLCTS
GTAAANYYPAVCEAFHSRVPLIVLTADRPHELRDVGAPQAMNQFNLYGTFVKQFTEMALPEASEAMYHYARMTTQRMIAS
ACLAPQGPVHLNFPVREPLIPDFSLESLWDKGRGEYTGVVQQGNVVMPSEYVDSLVGRLSHMEKGLIICGDDSHSEIAAF
ATQLAEKTGYPILADPLSNIRSGHHDKTMVIDCYDTFLRNELLKETWKPDVLIRFGGMPVSKALTQFIKKQTKAVHIVVD
ESGQWRDPALVATEVVQASDIAFCSALIEKMPVMKKNDWFRMWQHINEKTKETLREMETYDTAFEGRVITDIVRVLPEGA
TLFASNSMPIRDTDSFFFTSDKNIQVMANRGVNGIDGIISTALGASIICDPLVLVIGDLSFYHDLNGLLAAKLHELNITI
VVVNNDGGGIFSFLPQYEKKEHFESLFGTPIGLDYEHVVTMYGGSFSRVNGWEQFREEVQKGATTEGLHVVEICTNRDEN
LTLHRTLWAKTQDVITTSLQGESK

Sequences:

>Translated_584_residues
MNNHIEALSYYLGAFVDELTRLNVCDVVISPGSRSTPIALLMEQHEGMNTYLHVDERSAGFFALGIAKAKKRPVALLCTS
GTAAANYYPAVCEAFHSRVPLIVLTADRPHELRDVGAPQAMNQFNLYGTFVKQFTEMALPEASEAMYHYARMTTQRMIAS
ACLAPQGPVHLNFPVREPLIPDFSLESLWDKGRGEYTGVVQQGNVVMPSEYVDSLVGRLSHMEKGLIICGDDSHSEIAAF
ATQLAEKTGYPILADPLSNIRSGHHDKTMVIDCYDTFLRNELLKETWKPDVLIRFGGMPVSKALTQFIKKQTKAVHIVVD
ESGQWRDPALVATEVVQASDIAFCSALIEKMPVMKKNDWFRMWQHINEKTKETLREMETYDTAFEGRVITDIVRVLPEGA
TLFASNSMPIRDTDSFFFTSDKNIQVMANRGVNGIDGIISTALGASIICDPLVLVIGDLSFYHDLNGLLAAKLHELNITI
VVVNNDGGGIFSFLPQYEKKEHFESLFGTPIGLDYEHVVTMYGGSFSRVNGWEQFREEVQKGATTEGLHVVEICTNRDEN
LTLHRTLWAKTQDVITTSLQGESK
>Mature_584_residues
MNNHIEALSYYLGAFVDELTRLNVCDVVISPGSRSTPIALLMEQHEGMNTYLHVDERSAGFFALGIAKAKKRPVALLCTS
GTAAANYYPAVCEAFHSRVPLIVLTADRPHELRDVGAPQAMNQFNLYGTFVKQFTEMALPEASEAMYHYARMTTQRMIAS
ACLAPQGPVHLNFPVREPLIPDFSLESLWDKGRGEYTGVVQQGNVVMPSEYVDSLVGRLSHMEKGLIICGDDSHSEIAAF
ATQLAEKTGYPILADPLSNIRSGHHDKTMVIDCYDTFLRNELLKETWKPDVLIRFGGMPVSKALTQFIKKQTKAVHIVVD
ESGQWRDPALVATEVVQASDIAFCSALIEKMPVMKKNDWFRMWQHINEKTKETLREMETYDTAFEGRVITDIVRVLPEGA
TLFASNSMPIRDTDSFFFTSDKNIQVMANRGVNGIDGIISTALGASIICDPLVLVIGDLSFYHDLNGLLAAKLHELNITI
VVVNNDGGGIFSFLPQYEKKEHFESLFGTPIGLDYEHVVTMYGGSFSRVNGWEQFREEVQKGATTEGLHVVEICTNRDEN
LTLHRTLWAKTQDVITTSLQGESK

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=553, Percent_Identity=28.75226039783, Blast_Score=207, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 65056; Mature: 65056

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNHIEALSYYLGAFVDELTRLNVCDVVISPGSRSTPIALLMEQHEGMNTYLHVDERSAG
CCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEECCCCCCEEEEEECCCCC
FFALGIAKAKKRPVALLCTSGTAAANYYPAVCEAFHSRVPLIVLTADRPHELRDVGAPQA
EEEEEEHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHCCCCHH
MNQFNLYGTFVKQFTEMALPEASEAMYHYARMTTQRMIASACLAPQGPVHLNFPVREPLI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
PDFSLESLWDKGRGEYTGVVQQGNVVMPSEYVDSLVGRLSHMEKGLIICGDDSHSEIAAF
CCCCHHHHHHCCCCCEEEEEECCCEECCHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHH
ATQLAEKTGYPILADPLSNIRSGHHDKTMVIDCYDTFLRNELLKETWKPDVLIRFGGMPV
HHHHHHHCCCCEEECCHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCH
SKALTQFIKKQTKAVHIVVDESGQWRDPALVATEVVQASDIAFCSALIEKMPVMKKNDWF
HHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
RMWQHINEKTKETLREMETYDTAFEGRVITDIVRVLPEGATLFASNSMPIRDTDSFFFTS
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEC
DKNIQVMANRGVNGIDGIISTALGASIICDPLVLVIGDLSFYHDLNGLLAAKLHELNITI
CCCEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHHHEEEEEEEEE
VVVNNDGGGIFSFLPQYEKKEHFESLFGTPIGLDYEHVVTMYGGSFSRVNGWEQFREEVQ
EEEECCCCCHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHEEECCCCHHCCCHHHHHHHHH
KGATTEGLHVVEICTNRDENLTLHRTLWAKTQDVITTSLQGESK
CCCCCCCEEEEEEECCCCCCEEEEHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MNNHIEALSYYLGAFVDELTRLNVCDVVISPGSRSTPIALLMEQHEGMNTYLHVDERSAG
CCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEECCCCCCEEEEEECCCCC
FFALGIAKAKKRPVALLCTSGTAAANYYPAVCEAFHSRVPLIVLTADRPHELRDVGAPQA
EEEEEEHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHCCCCHH
MNQFNLYGTFVKQFTEMALPEASEAMYHYARMTTQRMIASACLAPQGPVHLNFPVREPLI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
PDFSLESLWDKGRGEYTGVVQQGNVVMPSEYVDSLVGRLSHMEKGLIICGDDSHSEIAAF
CCCCHHHHHHCCCCCEEEEEECCCEECCHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHH
ATQLAEKTGYPILADPLSNIRSGHHDKTMVIDCYDTFLRNELLKETWKPDVLIRFGGMPV
HHHHHHHCCCCEEECCHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCH
SKALTQFIKKQTKAVHIVVDESGQWRDPALVATEVVQASDIAFCSALIEKMPVMKKNDWF
HHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
RMWQHINEKTKETLREMETYDTAFEGRVITDIVRVLPEGATLFASNSMPIRDTDSFFFTS
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEC
DKNIQVMANRGVNGIDGIISTALGASIICDPLVLVIGDLSFYHDLNGLLAAKLHELNITI
CCCEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHHHEEEEEEEEE
VVVNNDGGGIFSFLPQYEKKEHFESLFGTPIGLDYEHVVTMYGGSFSRVNGWEQFREEVQ
EEEECCCCCHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHEEECCCCHHCCCHHHHHHHHH
KGATTEGLHVVEICTNRDENLTLHRTLWAKTQDVITTSLQGESK
CCCCCCCEEEEEEECCCCCCEEEEHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA