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The map label for this gene is lipA [H]

Identifier: 52140559

GI number: 52140559

Start: 4794418

End: 4795314

Strand: Reverse

Name: lipA [H]

Synonym: BCZK4698

Alternate gene names: 52140559

Gene position: 4795314-4794418 (Counterclockwise)

Preceding gene: 52140557

Following gene: 52140561

Centisome position: 90.46

GC content: 38.68

Gene sequence:

>897_bases
ATGACAAAACAAACAGAATATAAGCGCAAGCCCGAATGGTTGAAAATTAAGTTAAACACGAATGAAAACTATACAGGCTT
AAAGAAAATGATGCGTTCTAAGAATCTTCATACAGTTTGTGAAGAAGCGAAATGTCCGAATATTCATGAATGCTGGGCTG
TAAGAAAAACAGCAACATTTATGATTTTAGGAGCAGTATGTACACGTGCTTGTCGTTTTTGTGCGGTTAAAACAGGCTTA
CCAACAGAGCTTGATTTACAAGAGCCAGAACGCGTAGCAGATTCTGTAGTACAAATGGGCTTAAAGCACGTTGTTATAAC
AGCGGTTGCACGTGATGATTTAAAAGACGGGGGAGCAGCTGTTTTTGCTGAAACAGTACGCGCTGTTCGTCGTAAAAATC
CATTTACGTCAATCGAAGTATTACCATCTGATATGGGTGGAGTAGAAGAAAACTTAAAAATGTTAATGGATGCAAAACCA
GATATTTTAAACCATAACATTGAAACAGTACGTCGATTATCTGACCGAGTTCGCGCTAGAGCAAAATATGATCGTTCATT
AGAGTTTTTACGTCGAGCGAAAGAAATGCAGCCTGATATTCCAACTAAATCGAGCATTATGGTGGGCTTAGGTGAAACGA
GAGAAGATTTAATTGAAGCAATGGATGACTTACGTGCAAACAATGTGGATATTTTAACTCTTGGACAATACCTACAACCA
TCTAAGAAGCATTTACCAGTTCTTAAATATTACCCACCAGCAGAATTTGCAGAACTTAAAGAAATTGCACTTAGCAAAGG
ATTTAGCCACTGTGAAGCTGGCCCACTTGTGCGTTCTTCTTATCATGCGGACGAGCAAGTTCGTTCTGCAAAAGAAAACA
CAGCAGAAGCAAAATAA

Upstream 100 bases:

>100_bases
TCTTCATTTGAGCAAATCGCTTTCATACGTTAGAATAGATATGTTACATTGAAAGAAGCGAACACGGTAAACTATTTCAT
ATTGTGCGGAGTTGATAACC

Downstream 100 bases:

>100_bases
TGAAAAAAGGGAGCTAACGATTAGCTCTCTTTTTGTTTGTACTCCAAAACAGTATTCGAACATGTTAATAAAATCTTTTT
TAATTTCTCTGTGGATAATT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTKQTEYKRKPEWLKIKLNTNENYTGLKKMMRSKNLHTVCEEAKCPNIHECWAVRKTATFMILGAVCTRACRFCAVKTGL
PTELDLQEPERVADSVVQMGLKHVVITAVARDDLKDGGAAVFAETVRAVRRKNPFTSIEVLPSDMGGVEENLKMLMDAKP
DILNHNIETVRRLSDRVRARAKYDRSLEFLRRAKEMQPDIPTKSSIMVGLGETREDLIEAMDDLRANNVDILTLGQYLQP
SKKHLPVLKYYPPAEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVRSAKENTAEAK

Sequences:

>Translated_298_residues
MTKQTEYKRKPEWLKIKLNTNENYTGLKKMMRSKNLHTVCEEAKCPNIHECWAVRKTATFMILGAVCTRACRFCAVKTGL
PTELDLQEPERVADSVVQMGLKHVVITAVARDDLKDGGAAVFAETVRAVRRKNPFTSIEVLPSDMGGVEENLKMLMDAKP
DILNHNIETVRRLSDRVRARAKYDRSLEFLRRAKEMQPDIPTKSSIMVGLGETREDLIEAMDDLRANNVDILTLGQYLQP
SKKHLPVLKYYPPAEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVRSAKENTAEAK
>Mature_297_residues
TKQTEYKRKPEWLKIKLNTNENYTGLKKMMRSKNLHTVCEEAKCPNIHECWAVRKTATFMILGAVCTRACRFCAVKTGLP
TELDLQEPERVADSVVQMGLKHVVITAVARDDLKDGGAAVFAETVRAVRRKNPFTSIEVLPSDMGGVEENLKMLMDAKPD
ILNHNIETVRRLSDRVRARAKYDRSLEFLRRAKEMQPDIPTKSSIMVGLGETREDLIEAMDDLRANNVDILTLGQYLQPS
KKHLPVLKYYPPAEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVRSAKENTAEAK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=289, Percent_Identity=44.9826989619377, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI37577164, Length=251, Percent_Identity=45.8167330677291, Blast_Score=227, Evalue=9e-60,
Organism=Escherichia coli, GI1786846, Length=290, Percent_Identity=52.0689655172414, Blast_Score=302, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI32564533, Length=286, Percent_Identity=45.4545454545455, Blast_Score=238, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6324770, Length=286, Percent_Identity=46.8531468531469, Blast_Score=261, Evalue=7e-71,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=46.2897526501767, Blast_Score=254, Evalue=4e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 33688; Mature: 33557

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQTEYKRKPEWLKIKLNTNENYTGLKKMMRSKNLHTVCEEAKCPNIHECWAVRKTATF
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHH
MILGAVCTRACRFCAVKTGLPTELDLQEPERVADSVVQMGLKHVVITAVARDDLKDGGAA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
VFAETVRAVRRKNPFTSIEVLPSDMGGVEENLKMLMDAKPDILNHNIETVRRLSDRVRAR
HHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHH
AKYDRSLEFLRRAKEMQPDIPTKSSIMVGLGETREDLIEAMDDLRANNVDILTLGQYLQP
HHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECCHHHCC
SKKHLPVLKYYPPAEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVRSAKENTAEAK
HHHCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TKQTEYKRKPEWLKIKLNTNENYTGLKKMMRSKNLHTVCEEAKCPNIHECWAVRKTATF
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHH
MILGAVCTRACRFCAVKTGLPTELDLQEPERVADSVVQMGLKHVVITAVARDDLKDGGAA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
VFAETVRAVRRKNPFTSIEVLPSDMGGVEENLKMLMDAKPDILNHNIETVRRLSDRVRAR
HHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHH
AKYDRSLEFLRRAKEMQPDIPTKSSIMVGLGETREDLIEAMDDLRANNVDILTLGQYLQP
HHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECCHHHCC
SKKHLPVLKYYPPAEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVRSAKENTAEAK
HHHCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA